Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 2,413,362 G→A 7.7% A469T (GCG→ACG)  ctpH → Methyl‑accepting chemotaxis protein CtpH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,413,3620GA 7.7% 163.1 / 3.0 52A469T (GCG→ACG) ctpHMethyl‑accepting chemotaxis protein CtpH
Reads supporting (aligned to +/- strand):  ref base G (30/18);  new base A (2/2);  total (32/20)
Fisher's exact test for biased strand distribution p-value = 6.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACAGTCAGGGGCTGTGGAGGCGGTCAAGGCCATCGAAAGTGGCCAGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAATTACCCGAATTGCCACGGCCAACCAGGAAGCGGTG  >  ACB122/2413249‑2413493
                                                                                                                 |                                                                                                                                   
tACAGTCAGGGGCTGTGGAGGCGGTCAAGGCCATCGAAAGTGGCCAGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCcg                                                                                                                     >  3:287022/1‑130 (MQ=255)
tACAGTCAGGGGCTGTGGAGGCGGTCAAGGCCATCGAAAGTGGCCAGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCcg                                                                                                                     <  4:287022/130‑1 (MQ=255)
        ggggCTGTGGAGGCGGTCAAGGCCATCGAAAGTGGCCAGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCg                                                                                                         >  1:353834/1‑134 (MQ=255)
             tgtgGAGGTGGTCAAGGCCATCGAAAGTGGCCAGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCg                                                                                                         >  5:18653/1‑129 (MQ=255)
             tgtgGAGGCGGTCAAGGCCATCGAAAGTGGCCAGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCg                                                                                                         >  6:128906/1‑129 (MQ=255)
             tgtgGAGGCGGTCAAGGCCATCGAAAGTGGCCAGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCg                                                                                                         >  2:93800/1‑129 (MQ=255)
             tgtgGAGGCGGTCAAGGCCATCGAAAGTGGCCAGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCg                                                                                                         >  5:363569/1‑129 (MQ=255)
                  aGGCGGTCAAGGCCATCGAAAGTGGCCAGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTNTCACGCAGACGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCca                                                                                              >  7:323130/1‑135 (MQ=255)
                  aGGCGGTCAAGGCCATCGAAAGTGGCCAGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGcc                                                                                               >  1:358165/1‑134 (MQ=255)
                  aGGCGGTCAAGGCCATCGAAAGTGGCCAGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCca                                                                                              >  8:58670/1‑135 (MQ=255)
                  aGGCGGTCAAGGCCATCGAAAGTGGCCAGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCca                                                                                              >  3:172638/1‑135 (MQ=255)
                  aGGCGGTCAAGGCCATCGAAAGTGGCCAGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGACGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCca                                                                                              >  7:317585/1‑135 (MQ=255)
                        tCAAGGCCAACGAAAGGGGCCAGCAACGTAGCCAGGACGGGGCTGAGCAAGTCGCGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCgg                                                                                        <  2:353834/135‑1 (MQ=255)
                          aaGGCCATCGAAAGTGGCCAGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGcc                                                                                      >  3:99752/1‑135 (MQ=255)
                             gCCATCGAAAGTGGACAGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCgag                                                                                   <  2:312537/135‑1 (MQ=255)
                                    aaaGTGGCCAGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGa                                                                            >  1:307768/1‑135 (MQ=255)
                                         ggCCAGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGc                                                                       >  5:214080/1‑135 (MQ=255)
                                         ggCCAGCAACGTAGCCAGGAAGGCGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGc                                                                       >  1:290697/1‑135 (MQ=255)
                                           ccAGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGt                                                                     >  5:299322/1‑135 (MQ=255)
                                           ccAGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGGGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGt                                                                     <  6:93732/135‑1 (MQ=255)
                                             aGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGt                                                                     >  6:126144/1‑133 (MQ=255)
                                             aGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGt                                                                     <  5:126144/133‑1 (MQ=255)
                                             aGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTgg                                                                   >  6:222318/1‑135 (MQ=255)
                                             aGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTgg                                                                   >  5:44368/1‑135 (MQ=255)
                                             aGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGc                                                                  >  7:366545/1‑136 (MQ=255)
                                                  cGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCgag                                                                                   <  6:169500/114‑1 (MQ=255)
                                                  cGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCgag                                                                                   >  5:169500/1‑114 (MQ=255)
                                                                   tGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAAcc                                              >  1:72530/1‑134 (MQ=255)
                                                                   tGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGGGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCt                                             <  5:222318/135‑1 (MQ=255)
                                                                         aGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGa                                       <  6:44368/135‑1 (MQ=255)
                                                                                     cGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAATTACCCGAAtt                          <  4:172638/136‑1 (MQ=255)
                                                                                      gggCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAATTACCCGAATTg                         >  3:70186/1‑136 (MQ=255)
                                                                                       ggCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAATTACCCGAATTg                         >  5:201611/1‑135 (MQ=255)
                                                                                           gATGCTGCACCGTATCACGCAGGCGGTGCAAGCGAGCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAATTACCCGAATTGCCAc                     <  1:176660/135‑1 (MQ=255)
                                                                                            aTGCTGCAACGTATCACGCAGGCGGTGGATGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAATTACCCGAATTGCCACg                    <  6:363569/135‑1 (MQ=255)
                                                                                            aTGCTGCAACGTATCACGAAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAATTACCCGAATTGCCACg                    <  2:290697/135‑1 (MQ=255)
                                                                                            aTGCTGAAAGGTATCACGCAGGCGGTGGACGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAATTACCCGAATTGCCACg                    <  6:214080/135‑1 (MQ=255)
                                                                                             tgctgcAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAATTACCCGAATTGCCACGGc                  <  7:225951/136‑1 (MQ=255)
                                                                                                tgcAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAATTACCCGAATTGCCACGGCCaa               >  3:309067/1‑136 (MQ=255)
                                                                                                 gcAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAATTACCCGAATTGCCACGGCCAAc              >  7:343317/1‑136 (MQ=255)
                                                                                                     cGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAATTACCCGAATTGCCACGGCCAACCAg           >  8:84788/1‑135 (MQ=255)
                                                                                                     cGTATCACGCAGACGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAATTACCCGAATTGCCACGGCCAACCAgg          <  8:317585/136‑1 (MQ=255)
                                                                                                     cGTATCACGCAGACGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAATTACCCGAATTGCCACGGCCAACCAgg          <  8:323130/136‑1 (MQ=255)
                                                                                                        aTCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAATTACCCGAATTGCCACGGCCAACCAGGaa        <  1:213784/135‑1 (MQ=255)
                                                                                                          cACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAAtt                                   >  2:394230/1‑106 (MQ=255)
                                                                                                          cACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAAtt                                   <  1:394230/106‑1 (MQ=255)
                                                                                                           actcAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAATTACCCGAATTGCCACGGCCAACCAGGAAGCg     <  4:99752/132‑1 (MQ=255)
                                                                                                           aCGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAATTACCCGAATTGCCACGGCCAACCAGGAAGCg     >  6:334917/1‑135 (MQ=255)
                                                                                                             gCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAATTACCCGAATTGCCACTGCCAACCAGGaa        <  4:182803/130‑1 (MQ=255)
                                                                                                             gCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAATTACCCGAATTGCCACTGCCAACCAGGaa        >  3:182803/1‑130 (MQ=255)
                                                                                                             gCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAATTACCCGAATTGCCACGGCCAACCAGGAAGCGGTg  >  7:164749/1‑136 (MQ=255)
                                                                                                               aGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAATTACCCGAATTGCCACGGCCAACCAGGAAGCGGt   >  2:112571/1‑133 (MQ=255)
                                                                                                                 |                                                                                                                                   
TACAGTCAGGGGCTGTGGAGGCGGTCAAGGCCATCGAAAGTGGCCAGCAACGTAGCCAGGAAGGGGCTGAGCAAGTCGAGCAGGCCGGGCAGATGCTGCAACGTATCACGCAGGCGGTGGAAGCGATCCGCGACATGAACCGGCAGATCGCCACAGCGGCCGAGGAGCAGACCAGCGTGGCCGAAGATATCTCGCGCAACCTGATCGAAATTACCCGAATTGCCACGGCCAACCAGGAAGCGGTG  >  ACB122/2413249‑2413493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑