Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	2,710,414	A→T	5.3%	Y691F (TAC→TTC)	CJ019_02558 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	2,710,414	0	A	T	5.3%	234.1 / 2.7	76	Y691F (TAC→TTC)	CJ019_02558	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (41/30);  new base T (2/2);  total (44/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

GACGGCCAGCGTCAGGTGTACAGCCTGCTGCGCAACCGCGCGCACAGCAACGTGGCGTTCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAGTTCGAGCGCATTGTCATGCGCTGGGGTGTGCGCCGCAGTCACC  >  ACB122/2710281‑2710542                                                                                                                                      |                                                                                                                                 gACGGCCAGCGTCAGGTGTACAGCCTGCTGCGCAACCGCGCGCACAGCAACGTGGCGTTCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTAc                                                                                                                                 >  2:117064/1‑135 (MQ=255)     gCCAGCGTCAGGTGTACAGCCTGCTGCGCAACCGCGCGCACAGCAACGTGGCGTTCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGa                                                                                                                             <  5:207679/135‑1 (MQ=255)                   tACAGCCTGCTGCGCAACCGCGCGCACAGCAACGTGGCGTTCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAAc                                                                                                               <  8:317889/135‑1 (MQ=255)                       gCCTGCTGCGCAACCGCGCGCACAGCAACGTGGCGTTCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATccc                                                                                                          >  3:380693/1‑136 (MQ=255)                       gCCTGCTGCGCAACCGCGCGCACAGCAACGTGGCGTTCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATcc                                                                                                           >  1:156624/1‑135 (MQ=255)                       gCCTGCTGCGCAACCGCGCGCACAGCAACGTGGCGTTCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGCGCTGGATACCCTGACCCTGTACACGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATcc                                                                                                           >  5:194705/1‑135 (MQ=255)                        ccTGCTGCGCAACCGCGCGCACAGCAACGTGGCGTTCCTGCTGGGTGAGGCGTACCGCTACGAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGGTACCCGAACTTCATCTTCAACATCCCg                                                                                                         <  3:156921/136‑1 (MQ=255)                        ccTGCTGCGCAACCGCGCGCACAGCAACGTGGCGTTCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATccc                                                                                                          >  4:141266/1‑135 (MQ=255)                             tgcGCAACCGCGCGCACAGCAACGTGGCGTTCCTGCTGGGTGCGGCGTACCGCTACCAGCCGGGGCTGGATCCCCTGACCCTGTCCCCGGGGGTGCTCTCCAGCGCCCCGAGCTTCAGCGTCACCATCCCgcc                                                                                                       >  5:110453/1‑131 (MQ=255)                             tgcGCAACCGCGCGCACAGCAACGTGGCGTTCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGAc                                                                                                       >  5:381501/1‑133 (MQ=255)                              gcgcAACCGCGCGCACAGCAACGTGGCGTTCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCaa                                                                                                    >  6:286788/1‑135 (MQ=255)                                     cacgcgcACAGCAACGTGGCGTTCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGAtggg                                                                                             >  1:375922/3‑133 (MQ=255)                                          gcACAGCAACGTGGCGTTCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATATGCCGGa                                                                                        >  1:55992/1‑135 (MQ=255)                                                cAACGTGGCGTTCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTtcgtc                                                                                 <  3:96321/136‑1 (MQ=255)                                                   cGTGGCGTTCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGa                                                                               >  2:158257/1‑135 (MQ=255)                                                    gTGGCGTTCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAgg                                                                             <  7:392598/136‑1 (MQ=255)                                                    gTGGCGTTCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAg                                                                              <  2:233702/135‑1 (MQ=255)                                                     tGGCGTTCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAgg                                                                             <  1:120488/135‑1 (MQ=255)                                                     tGGCGTTCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAgg                                                                             <  5:286788/135‑1 (MQ=255)                                                        cGTTCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACa                                                                          >  1:272570/1‑135 (MQ=255)                                                         gTTCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACagg                                                                        >  7:79453/1‑134 (MQ=255)                                                          ttCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATg                                                                        <  1:320120/135‑1 (MQ=255)                                                           tCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAgg                                                                             <  8:206946/129‑1 (MQ=255)                                                           tCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAgg                                                                             >  7:206946/1‑129 (MQ=255)                                                           tCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACAt                                                                         >  6:89072/1‑133 (MQ=255)                                                           tCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTAATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATg                                                                        >  7:276780/1‑134 (MQ=255)                                                           tCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCCCACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACAt                                                                         >  5:54971/1‑133 (MQ=255)                                                           tCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACACGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACAt                                                                         >  6:338800/1‑133 (MQ=255)                                                             ctgctgGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAg                                                                                                   >  2:248689/1‑105 (MQ=255)                                                             ctgctgGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAg                                                                                                   <  1:248689/105‑1 (MQ=255)                                                             ctgctgGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGa                                                                      >  6:201356/1‑134 (MQ=255)                                                             ctgctgGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGa                                                                      >  1:330270/1‑134 (MQ=255)                                                             ctgctgGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAg                                                                     >  4:134545/1‑135 (MQ=255)                                                                        ggCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGa                                                          >  2:79334/1‑135 (MQ=255)                                                                           gTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGAc                                                      >  3:99702/1‑136 (MQ=255)                                                                            tACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCATGGAGGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATgcc                                                      >  6:241908/1‑133 (MQ=255)                                                                             acggCTACCAGCCGGGGCTGGAAACCCTGACCCTGTACCCGGGTGTGCTCTCCACCTACCCGAACTTAATCTTCAACTTCACGACCAAGGATGTGCCGGAGTTCGTCGAGGACTTGGAGTACGCCAAAGATGAcg                                                     <  2:375922/132‑1 (MQ=255)                                                                                gCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATg                                                                        >  7:178283/1‑113 (MQ=255)                                                                                gCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATg                                                                        <  8:178283/113‑1 (MQ=255)                                                                                gCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACgcggc                                                 >  8:207161/1‑136 (MQ=255)                                                                                gCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACgcgg                                                  <  6:172739/135‑1 (MQ=255)                                                                                    ccAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACAt                                                                         >  8:158986/1‑108 (MQ=255)                                                                                    ccAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACAt                                                                         <  7:158986/108‑1 (MQ=255)                                                                                    ccAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAg                                             <  3:112496/136‑1 (MQ=255)                                                                                     cAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTGCGGCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAg                                             <  2:156624/135‑1 (MQ=255)                                                                                      aGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGAGGTGCCGGAGTTCGTCGAGGACATGGCGTACGCCAAAGATGACGCGGCGAAGtt                                           >  3:252411/1‑136 (MQ=255)                                                                                          ggggCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTTCCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAGTTCGAg                                       >  3:170616/1‑136 (MQ=255)                                                                                          ggggCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTTCCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAGTTCGAg                                       >  3:164299/1‑136 (MQ=255)                                                                                          ggggCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAGTTCGAg                                       <  7:261718/136‑1 (MQ=255)                                                                                            ggCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATTGAGTACGCCAAAGATGACGCGGCGAAGTTCGAgc                                      <  6:209390/135‑1 (MQ=255)                                                                                                ggATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTTCCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAGTTCGAGCGCAtt                                 <  4:164299/136‑1 (MQ=255)                                                                                                 gATACCCTGACCCTGTACCGGGGTGTGCTCTCCAGCTACCCGAACTTCATCTTCTACATCCCGACCAAGGATCTGCCGGAGTTCGTCGAGGACATGGATTACGCCAAAGATGACGCGGCGAAGTTCGAGCGCAtt                                 <  8:68441/135‑1 (MQ=255)                                                                                                 gATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTTCCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAGTTCGAGCGCAtt                                 <  4:170616/135‑1 (MQ=255)                                                                                                 gATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAGTTCGAGCGCAtt                                 <  2:272570/135‑1 (MQ=255)                                                                                                 gATACCCTGACCCTGTACCCGGGGGTGCGCTCCAGCTACCCGAACTTCATCTGCAACATCCCGACCAAGGATGTGCCGGAGTGCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAGTTCGAGCGCAtt                                 <  6:291845/135‑1 (MQ=255)                                                                                                  aTACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAGTTCGAGCGCATTGt                               <  4:375494/136‑1 (MQ=255)                                                                                                      cctgaccctgTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAGTTCGAGCGCATTGTCAt                            <  1:158257/135‑1 (MQ=255)                                                                                                        tgaccctgTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCGGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAGTTCGAGCGCATTGTCATgc                          <  6:197470/135‑1 (MQ=255)                                                                                                            cctgTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAGTTCGAGCGCATTGTCATGCGCTgg                     <  4:77166/136‑1 (MQ=255)                                                                                                            cctgTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAGTTCGAGCGCATTGTCATGCGCTg                      >  2:118687/1‑135 (MQ=255)                                                                                                            cctgTACCCGGGGCGGCTCTCCATCTACCCGAACTTCATCTTCAACATCCCGACCAAGGCTGTGCCGGCGTTCGTCGAGGACATGGACTCCGCCAAAGATGACGCGGCGAAGTTCGATCGCATTGTCATGCGCTgg                     <  7:371589/136‑1 (MQ=255)                                                                                                              tgTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAGTTCGAGCGCATTGTCATGCGCTgggg                   >  4:26929/1‑136 (MQ=255)                                                                                                              tgTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAGTTCGAGCGCATTGTCATGCGCTgggg                   >  7:43057/1‑136 (MQ=255)                                                                                                                 aCCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTtcg                                                                                   >  3:107860/1‑69 (MQ=255)                                                                                                                 aCCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTtcg                                                                                   <  4:107860/69‑1 (MQ=255)                                                                                                                  cccGGGGGTGCTCTCCATCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAGTTCGAGCGCATTGTCATGCGCTGGGgtgt                >  6:368386/1‑135 (MQ=255)                                                                                                                  cccGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTAAGCCAAAGATGACGCGGCGCAGTTCGAGCGCATTGTCATGCGCTGGGGtgtg               >  4:298584/1‑136 (MQ=255)                                                                                                                   ccGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTtcg                                                                                   >  1:124085/1‑67 (MQ=255)                                                                                                                   ccGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTtcg                                                                                   <  2:124085/67‑1 (MQ=255)                                                                                                                   ccGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAgg                                                                             >  5:231580/1‑73 (MQ=255)                                                                                                                   ccGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAgg                                                                             <  6:231580/73‑1 (MQ=255)                                                                                                                   ccGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAGTTCGAGCGCATTGTCATGCGCTGGGGTGTgc              >  7:217806/1‑136 (MQ=255)                                                                                                                             tctcCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAGTTCGAGCGCATTGTCATGCGCTGGGGTGTGCGCCGCAGTc     >  5:49574/1‑135 (MQ=255)                                                                                                                             tctcCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAGTTCGAGCGCATTGTCATGCGCTGGGGTGTGCGCCGCAGTCa    >  4:187007/1‑136 (MQ=255)                                                                                                                               tcCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAGTTCGAGCGCATTGTCATGCGCTGGGGTGTGCGCCGCAGTCAc   >  6:12735/1‑135 (MQ=255)                                                                                                                                ccAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAGTTCGAGCGCAGTGTCATGCGCTGGGGTGTGCGCCGCAGTCAcc  >  5:125008/1‑135 (MQ=255)                                                                                                                                      |                                                                                                                                 GACGGCCAGCGTCAGGTGTACAGCCTGCTGCGCAACCGCGCGCACAGCAACGTGGCGTTCCTGCTGGGTGAGGCGTACCGCTACCAGCCGGGGCTGGATACCCTGACCCTGTACCCGGGGGTGCTCTCCAGCTACCCGAACTTCATCTTCAACATCCCGACCAAGGATGTGCCGGAGTTCGTCGAGGACATGGAGTACGCCAAAGATGACGCGGCGAAGTTCGAGCGCATTGTCATGCGCTGGGGTGTGCGCCGCAGTCACC  >  ACB122/2710281‑2710542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑