| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 2,788,018 | T→G | 38.1% | intergenic (+69/+43) | CJ019_02634 → / ← puuB_1 | hypothetical protein/Gamma‑glutamylputrescine oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 2,788,018 | 0 | T | G | 38.1% | 17.0 / 2.6 | 25 | intergenic (+69/+43) | CJ019_02634/puuB_1 | hypothetical protein/Gamma‑glutamylputrescine oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (8/7); new base G (4/6); total (12/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGCCGGGCAAGAGATTGCGGCGAAGCAGGCGGTCAATAGCCTGAACGAATAGCGTTGCCTGTGCCGGCTTCTTCGCCGGCAAGCCCGCGCCCACAGGATTTGTGCTTATTGCACAGTCCGTGTGGGCGCAGGT‑TTGCCGGCGAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATCGCGCAGGCTGTAGTACCAGGCACCGATGGCGCTGAAGGGTACGCGCAGCATCTGGCCACCCGGGAACGGGTAGT > ACB122/2787897‑2788152 | aGGCCGGGCAAGAGATTGCGGCGAACCAGGCGGTCAATAGCCTGAACGAATCGCGTTGCCTGTGCCGGCTTCTTCGCCGGCAAGCCCGCGCCCACAGGATTTGTGCTTATTGCACAGTCCGTGTGGGCGCAGGt‑t < 2:242554/135‑1 (MQ=255) ggCCGGGCAAGAGATTGCGGCGAAGCAGGCGGTCAATAGCCTGAACGAATAGCGTTGCCTGTGCCGGCTTCTTCGCCGGCAAGCCCGCGCCCCCAGGATTTTTGCTTATTGGACAGTCCGGGTGGGGGCAGGG‑TTg > 8:75537/1‑136 (MQ=255) aaGAGATTGCGGCGAAGCAGGCGGTCAATAGCCTGAACGAATAGCGTTGCCTGTGCCGGCTTCTTCGCCGGCCAGCCCGCCCCCCAAGGGTTTTTGCTTATTGGACAGGACGGGTGGGGGCCGGG‑TTGgcggcgc > 6:374598/1‑134 (MQ=255) aaGAGATTGCGGCGAAGCAGGCGGTCAATAGCCTGAACGAATAGCGTTGCCTGTGCCGGCTTCTTCGCCGGCAAGCCCGCGCCCCCAGGATTTGTGCTTATTGCCCAGTCCGGGTGGGCGCAGGT‑TTGgcggcg > 7:327613/1‑134 (MQ=255) aaGAGATTGCGGCGAAGCAGGCGGTCAATAGCCTGAACGAATAGCGTTGCCTGTGCCGGCTTCATCGCCGCCAAACCCGCCCCCCCAAGATATTTGCGTATTTCACCCTCCCTGTTGGCGCAAGTGTTGCCggga > 7:3075/1‑133 (MQ=255) gTCAATAGCCTGAACGAATAGCGTTGCCTGTGCCGGCTTCTTCGCCCGCAAACCCGCCCCCACAGGATTTTTGGGTATTGCAAAGTACGGGTGGGCGCAGGG‑TTGACGGGGAAGAGGCCCGGCCTGCCGGAATccg > 8:305259/1‑134 (MQ=255) ctaaTCGCGTGGCCGGGGCCGGCTGCTCCGCCGGCACGCCCGCGCCCACCGCATTGGGGCTTATTGCACAGCCCGGGGGGGCGCAGGT‑TGGCCGGCGAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATc < 8:14275/134‑1 (MQ=255) tAGCGTTGCCTGTGCCGGCTTCTTCGCCGGCAAGCCCGCGCCCACAGGATTTGTGCTTATTGCACAGTCCGTGTGGGCGCAGGT‑TTGCCGGCGAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATCgcgc < 8:381817/136‑1 (MQ=255) cgcgGGAAAGCCCGCCCCAACCGTTTTTGGGCTTATGCCACTGCCGGGGTGGGCGAAGGT‑TTGCGGGCTAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATCGCGCAGGCTGTAGTACCAGGCACCg < 3:230918/133‑1 (MQ=255) gCCCGCGCCCACCGGATTTGTGGGTATTGCACAGTCCGTGTGGGCGCAGGT‑TTGCCGGCGCAGAGGCCAGGCCTGCCGGAATCAGAAGGCCAGGCGATCGGGCCGGCGGTAGGTCCAGGCCCCGCTGGCGCTGaa > 2:102491/1‑135 (MQ=255) cgcCCACCGGATTTGGGCGCATTGCACAGCCCGGGGGGGCGCTGGT‑TGGCCGGCGAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATCGCGCAGGCTGTAGTACCAGGCACCGATGGCGCTGAAGGGTAc < 7:314802/136‑1 (MQ=255) cgcCCACAGGTTTTGCGCTAATTGCACCGTCGGGGTGGGGGCAGGT‑TGCCCGGCGAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCACGCGATCGCGCAGGCTGTAGTACCAGGCACCGATGGCGCTGAAGGGTAc < 3:67969/136‑1 (MQ=255) gcCCACCGGATTTGGCCTTATTGCACAGGCGGTGTGGGCGCCGGT‑TTGCGGGCGAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATCGCGCAGGCTGTAGTACCAGGCACCGATGGCGCTGAAGGGTAc < 6:82060/135‑1 (MQ=255) ttGTGCTTATTCCACAGTCCGTGTGGGCGCAGGT‑TTGCCGGCGAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATCGCGCAGGCTGTAGTACCAGGCACCGATGGCGCTGAAGGGTACGCGCAGCATCt < 6:202958/135‑1 (MQ=255) tgattATTGCACTGTCGGTGTGGGCGCAGGT‑TTGCCGGCGAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATCGCGCAGGCTGTAGTACCAGGCACCGATGGCGCTGAAGGGTACGCGCAGCATCTGGcc < 7:286282/133‑1 (MQ=255) gCTTATTGCACAGTCCGTGTGGGCGCAGGT‑TTGCCGGCGAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATCGCGCAGGCTGTAGTACCAGGCACCGATGGCGCTGAAGGGTACGCGCAGCATCTGGct > 6:28995/1‑134 (MQ=255) gCTTATTGCACAGTCCGTGTGGGCGCAGGT‑TTGCCGGCGAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATCGCGCAGGCTGTAGTACCAGGCACCGATGGCGCTGAAGGGTACGCGCAGCATCTGGcc > 5:216063/1‑135 (MQ=255) gCTTATTGCACAGTCCGTGTGGGCGCAGGT‑TTGCCGGCGAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATCGCGCAGGCTGTAGTACCAGGCACCGATGGCGCTGAAGGGTACGCGCAGCATCTGGCCa > 7:48937/1‑136 (MQ=255) gttATTCCACTGTCCGGGTGGGCGCAGTT‑TTGCCGGCTAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATCGCGCAGGCTGTAGTACCAGGCACCGATGGCGCTGAAGGGTACGCGCAGCATCTGGCCAc < 3:284814/135‑1 (MQ=255) gtgaccaGTCCGTGGGGGCGCAGGT‑TGGCCGGCGAAGAGGACAGGCCTGCCTGAATCAGAAGCCCAGGCGATCGCGCAGGCTGTAGTACCAGGCACCGATGGCGCTGAAGGGTACGCGCAGCATCTGGCCACCCg < 8:328956/130‑1 (MQ=255) tccacaGGCGGTGTGGGCGCAGGT‑TTGCCGGCTAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATCGCGCAGGCTGTAGTACCAGGCACCGATGGCGCTGAAGGGTACGCGCAGCATCTGGCCACCCg < 2:36150/132‑1 (MQ=255) tGCACAGTCCGTGTGGGCGCAGGT‑TTGCCGGCGAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATCGCGCAGGCTGTAGTACCAGGCACCGATGGCGCTGAAGGGTACGCGCAGCATCTGGCCACCCg > 2:233666/1‑134 (MQ=255) gccacaGTCCGGGTGGGCGCAGGT‑TGGCGGGCTAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATCGCGCAGGCTGTAGTACCAGGCACCGATGGCGCTGAAGGGTACGCGCAGCATCTGGCCACCCg < 5:75372/132‑1 (MQ=255) gCACAGTCCGTGTGGGCGCAGGT‑TTGCCGGCGAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATCGCGCAGGCTGTAGTACCAGGCACCGATGGCGCTGAAGGGTACGCGCAGCATCTGGCCACCCggg > 7:132640/1‑135 (MQ=255) ccGTGTGGGCGCAGGT‑TTGCCGGCGAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATCGCGCAGGCTGTAGTACCAGGCACCGATGGCGCTGAAGGGTACGCGCAGCATCTGGCCACCCGGGAACgg > 2:388640/1‑133 (MQ=255) gtgtGGGCGCAGGT‑TTGCCGGCGAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATCGCGCAGGCTGTAGTACCAGGCACCGATGGCGCTGAAGGGTACGCGCAGCATCTGGCCAc < 1:201411/121‑1 (MQ=255) gtgtGGGCGCAGGT‑TTGCCGGCGAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATCGCGCAGGCTGTAGTACCAGGCACCGATGGCGCTGAAGGGTACGCGCAGCATCTGGCCAc > 2:201411/1‑121 (MQ=255) gtgtGGGCGCAGGT‑TTGCCGGCGAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATCGCGCAGGCTGTAGTACCAGGCACCGATGGCGCTGAAGGGTACGCGCAGCATCTGGCCACCCGGGAACGGGTAg > 1:389927/1‑135 (MQ=255) gtgtGGGCGCAGGT‑TTGCCGGCGAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATCGCGCAGGCTGTAGTACCAGGCACCGATGGCGCTGAAGGGTACGCGCAGCATCTGGCCACCCGGGAACGGGTAg > 1:311136/1‑135 (MQ=255) gtgtGGGCGCAGGT‑TTGCCGGCGAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATCGCGCAGGCTGTAGTACCAGGCACCGATGGCGCTGAAGGGTACGCGCAGCATCTGGCCACCCGGGAACGGGTAGt > 7:189267/1‑136 (MQ=255) | AGGCCGGGCAAGAGATTGCGGCGAAGCAGGCGGTCAATAGCCTGAACGAATAGCGTTGCCTGTGCCGGCTTCTTCGCCGGCAAGCCCGCGCCCACAGGATTTGTGCTTATTGCACAGTCCGTGTGGGCGCAGGT‑TTGCCGGCGAAGAGGCCAGGCCTGCCTGAATCAGAAGCCCAGGCGATCGCGCAGGCTGTAGTACCAGGCACCGATGGCGCTGAAGGGTACGCGCAGCATCTGGCCACCCGGGAACGGGTAGT > ACB122/2787897‑2788152 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |