Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 2,847,839 T→A 22.5% N183K (AAT→AAA)  CJ019_02703 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,847,8390TA22.5% 73.4 / 6.6 43N183K (AAT→AAA) CJ019_02703hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (19/14);  new base A (2/8);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 6.89e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCAGAGTGGGCGCCCGGAGGTAGCGTTCGGCGCCTCAAAGTTCGCCCTCAACGGCATGGCTGATGCGCTGCGCGAAGGTTTCAGGGATGACAACCTGGCCGTGACCGTGCTCCAACTGGGTTATCTGAATACCGACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGCCAGGTACCGGTGCATGATGTGGTCACCATGGTCGACGCGCTGTTGCGCTTGTCCGGGGCTTCGTT  >  ACB122/2847709‑2847961
                                                                                                                                  |                                                                                                                          
gCCAGAGTGGGCGCCCGGAGGTAGCGTTCGGCGCCTCAAAGTTCGCCCTCAACGGCATGTCTGATGCGCTGCGCGAAGGTTTCAGGGATGACAACCTGGCCGTGACCGTGCTCCAACTGGGTTATCTGAATACcg                                                                                                                        >  1:25659/1‑135 (MQ=255)
 ccAGAGTGGGCGCCCGGAGGTAGCGTTCGGCGCCTCAAAGTTCGCCCTCAACGGCATGGCTGATGCGCTGCGCGAAGGTTTCAGGGATGACAACCTGGCCGTGACCGTGCTCCAACTGGGTTATCTGAATACcg                                                                                                                        <  6:170214/134‑1 (MQ=255)
 ccAGAGGGGGCGCCCGGAGTTAGCGTGGGGCGCCTAAATTTTCGCCCTCAACGTCTTGGCTGATGCGCTGCGCGATGGTTTCTGGTATGACACCGTGGCCGTGACCGGGCGCCAAATGGGTTTTCTGAATACcg                                                                                                                        <  2:51769/134‑1 (MQ=255)
  cAGAGTGGGCGCCCGGAGGTAGCGTTCGGCGCCTCAAAGTTCGCCCTCAACGGCATGGCTGATGCGCTGCGCGAAGGTTTCAGGGATGACAACCTGGCCGTGACCGTGCTCCAACTGGGTTATCTGAATACcgcc                                                                                                                      >  6:366854/1‑133 (MQ=255)
        gggCGCCCGGAGGTAGCGTTCGGCGCCTCAAAGTTCGCCCTCAACGGCATGGCTGATGCGCTGCGCGAAGGTTTCAGGGATGACAACCTGGCCGTGACCGTGCTCCAACTGGGTTATCTGAATACCGACGACTccc                                                                                                               >  3:122098/1‑136 (MQ=255)
           cgcCCGGAGGTAGCGTTCGGCGCCTCAAAGTTCGCCCTCAACGGCATGGCTGATGCGCTGCGCGAAGGTTTCAGGGATGACAACCTGGCCGTGACCGTGCTCCAACTGGGTTATCTGAATACCGACGACTCCCTGa                                                                                                            <  3:196530/136‑1 (MQ=255)
           cgcCCGGAGGTAGCGTTCGGCGCCGCAAAGTTCGCCCTCACCGGCATGGCTGATGCGCTGCGCGAAGGTTTCAGGGATGACAACCTGGCCGTGACCGTGCTCCAACGGGGTAATCTTAATACCGACGACTCCCTGa                                                                                                            <  3:372127/136‑1 (MQ=255)
            gcCCGGAGGTAGCGTTCGGCGCCTCAAAGTTCGCCCTCAACGGCATGGCTGATGCGCTGCGCGAAGGTTTCAGGGATGACAACCTGGCCGTGACCGTGCTCCCACTGGCTTATCTGAATACCGCCGACTCCCTGaa                                                                                                           >  7:134449/1‑136 (MQ=255)
            gcCCGGAGGTAGCGTTCGGCGCCTCAAAGTTCGCCCTCAACGGCATGGCTGATGCGCTGCGCGAAGGTTTCAGGGATGACAACCTGGCCGTGACCGTGCTCCAACTGGGTTATCTGAATACCGACGACTCCCTGaa                                                                                                           >  3:375452/1‑136 (MQ=255)
                           tCGGCGCCTCAAAGTTCGCCCTCAACGGCAAGGATGATGCGCTGCGCGAAGGTTTCAGGGATGACAACCTGGACGTGACCGTGCTACAACTGGGTTATCTGAAAACCGACGACTCCCATAACACGCCATTGGca                                                                                              >  2:221404/1‑134 (MQ=255)
                             ggCGCCTCAAAGTTCGCCCTCAACGGCATGGCTGATGCGCTGCGCGAAGGTTTCAGGGATGACAACCTGGCCGTGCCCGTGCTCCAACTGGGTTATCTGAATACCGACGACTCCCTGAACACGCCATTGGCACAAg                                                                                          >  3:19888/1‑136 (MQ=255)
                               cgcCTCAAAGTTCGCCCTCAACGGCATGGCTGATGCGCTGCGCGAAGGTTTCAGGGATGACAACCTGGCCGTGACCGTGCTCCAACTGGGTTATCTGAATACCGACGACTCCCTGAACACGCCATTGGCCCAAGc                                                                                         >  6:177724/1‑135 (MQ=255)
                               cgcCTCAAAGTTCGCCCTCAACGGCATGGCTGATGCGCTGCGCGAAGGTTTCAGGGATGACAACCTGGCCGTGACCGTGCTCCAACTGGGTTATCTGAATACCGACGACTCCCTGAACACGCCATTGGCACCAGc                                                                                         >  2:46106/1‑135 (MQ=255)
                               cgcCTCAAAGTTCGCCCTCAACGGCATGGCTGATGCGCTGCGCGAAGCTGTCAGGGATGACAACCTGGCCGTGACCGTGCTCCAACTGGGTTATCTGAATACCGACGACTGCCTGAACCCGCCCTTGGCACAAGcc                                                                                        >  4:281317/1‑136 (MQ=255)
                                      aagtcggCCCTCACCGGCTGGGTTGTTGCGGGGGGCGAAGGTTTCTGGCATGACACCGTGGCCGTGACCGTGCTCCATCTGGGTTATCTCAATCCCGACGACTCCCTGAACACGCCATTGGCACAAGCCgcag                                                                                    <  2:25659/127‑1 (MQ=255)
                                             cccTCACCGGCATGGCTGAGGCGCGGCGCGAAGGTTTCAGGGATCACACCCGGGCCGGGACCGGGCTCCAACTGGGTTATCTCAATCCCCACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGa                                                                          <  3:255803/136‑1 (MQ=255)
                                                  accGGCATGGCTGCTGCGCGGCGCGATGGTTTCAGGGATGACAACCTGGCCGGGACCGTGCTCCAACTGGGTTATCTGAATACCGACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAgcggc                                                                     <  7:387104/134‑1 (MQ=255)
                                                      gCATGGCTGAGGCGGTGCGCGACGGTTCCCGGCTTGACAACCTGGCGGGCCCCGGGCTCCAACTGGTTTAGCTCAATACCCACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGcc                                                                  <  1:43667/135‑1 (MQ=255)
                                                      gCAGGGCTGATGCGCGGCGCGAAGGTGGCTGGGAGGACAACGTGCCGGTGCCGGTGCCCCAACTGGTTTATCTGAATACCCACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGcc                                                                  <  6:300311/135‑1 (MQ=255)
                                                        aTGGCTGATGCGCTGCGCGAAGGTTTCAGGGATGACAACCTGGCCGTGACCGTGCTCCAACTGGGTTATCTGAATACCGACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGCCAgg                                                               <  4:375452/136‑1 (MQ=255)
                                                         tGGCTGATGCGCTGGGCTTAGGTTTAAGGGTTGACAACCTGGCCGTGACCGGGCTCCAACTGGGTTATCTGAAAACCGACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGCCAGGt                                                              <  4:385078/136‑1 (MQ=255)
                                                         gggCTGAGGCGCTGCGCGATGGTTTCTGGGATGACACCCTGGCCGTGACCGTGCTCCAAATGGGTTATCTTATAACCGACGACTCCCTGAACACGCCATTGGCACAATCAGCAGCAAGTGGCGAGCGGCGCCAGGt                                                              <  8:104529/135‑1 (MQ=255)
                                                          ggCTGATGCGCTGCGCGAAGGTTTCAGGGATGACAACCTGGCCGTGACCGTGCGCCAACTGGGTAATCTGAAAACCGACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGCCAGGTa                                                             <  8:20722/136‑1 (MQ=255)
                                                          ggCTGATGCGCGGCGCGACGGTTGCCGGGAGCACAACCGGGCGGTGACCGGGCGCCAAGTGGGTTATCTTAAACCCGACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGCCAGGt                                                              <  3:321416/135‑1 (MQ=255)
                                                          ggCTCAGGCGCTGCTCTATGTTTTCTGGCAACACAAACTGGCCGACACCGTGAACAACATGGTTTACAACAAAACCCACCACTCCCTGAACACGCCATTGGCACAAGACGCAGCAAGTGGCGAGCGGCGCCGGGt                                                              <  2:373467/135‑1 (MQ=255)
                                                           gCTGTTGCGCGGCGCGACGTTTTCAGGGATGACAACCTGGCCGTGACCGTGCTCCAACTGGGTTATCTTAATCCCGACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGCCAGGt                                                              <  1:42888/134‑1 (MQ=255)
                                                                 gcgctgcgcGAAGGTTGCAGGGATGACAACCTGGCCGTGACCGTGCTCCAACTGGGTTATCTGAAACCCGACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGCCAGGTACCGGTGc                                                      <  3:240141/136‑1 (MQ=255)
                                                                  cgctgcgcGAAGGTTTCAGGGATGACAACCTGGCCGTGCCCGTGCTCCAACTGGGTTATCTGAATACCGACGCCTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGCCAGGTACCCGTGCa                                                     >  4:234030/1‑136 (MQ=255)
                                                                  cgctgcgcGAAGGTTTCAGGGATGACAACCTGGCCGTGACCGTGCTCCAACTGGGTTATCTGAATACCGACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGCGCGGCGCCAGGTACCGGGGc                                                      >  5:222157/1‑135 (MQ=255)
                                                                   gctgcgcGAAGGTTTCAGGGATGACAACCTGGCCGTGACCGTGCTCCAACTGGGTTATCTGAATACCGACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGCCAGGTACCGGTGCat                                                    <  4:122098/136‑1 (MQ=255)
                                                                   gctgcgcGAAGGTGCCCGGTTAGACAACATGGCCGGCAGCGTGCCCAAAGGGGGTTATCTTAAAAGCGACGCCTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGCCAGGTACCGGTGCat                                                    <  7:260378/136‑1 (MQ=255)
                                                                   gcggcgcTATGGTTTCTGGGACGACAACCGGGCCGGGACCGTGCTCCAAGGGGTTTATCTTAAACCCGACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGCCAGGTACCGGTGCat                                                    <  3:292056/133‑1 (MQ=255)
                                                                   gcggcgcGATGGTTGCAGGGAGGACAACGTGGCCGTGACGGTGCTCCAAGTGGGTTATCTTAATACCCACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGCCAGGTACCGGTGCat                                                    <  8:134449/133‑1 (MQ=255)
                                                                        gcgAAGGTTTCAGGGATGACAACCTGGCCGTGCCCGTGCTCCAACTGGGTTATCTGAATACCGACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGCCAGGTACCGGTGCatgatgc                                               >  7:104529/1‑135 (MQ=255)
                                                                          gAAGGTTTCAGGGATGACAACCTGGCCGTGCCCGTGCTCCAACTGGGTTATCTGAATACCGACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGCCAGGTACCGGTGCATGAtgtg                                              >  6:75111/1‑135 (MQ=255)
                                                                           aaGGTTTCCGGGATGACAACCGGGCCGGGACCGGGCTCCACCTGGG‑TAGCTGAATCCCGACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGCCAGGTACCGGTGCATGATGTGGTc                                           <  3:273280/136‑1 (MQ=255)
                                                                                            aaCCTGGCCGTGACCGTGCTCCAACTGGGTTATCTGAATACCGACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGCCAGGTACCGGTGCATGATGTGGTCACCATGGTCGAcgcg                            >  6:209254/1‑135 (MQ=255)
                                                                                            aaCCTGGCCGTGACCGTGCTCCAACTGGGTTATCTGAAAACCGACGACTCCCTGAACCCGCCATTGGCACAACCCGCAGCAAGTGGCGAGCGGCGCCACGTACCGGTGCATGATGTGGTCACCATGGTCGAccc                             >  2:31304/1‑132 (MQ=255)
                                                                                                           gTGCTCCAACTGGGTTATCTGAATACCGACGACTCCCTGAACACGCCATTGGCCCACGCCGCAGCAAGTGGCGAGCGGCGCCAGGTACCGGTGCATGATGTGGTCACCCTGGTCGACGCGCTGTTGCGCTTGTCCg            >  8:260937/1‑136 (MQ=255)
                                                                                                           gTGCTCCAACTGGGTTATCTGAATACCGACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGCCAGGTACCGGTGCATGATGTGGTCACCATGGTCGACGCGCTGTTGCGCTTGTCCg            >  4:234350/1‑136 (MQ=255)
                                                                                                           gTGCTCCAACTGGGTTATCTGAATACCGACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGCCAGGTACCGGTGCATGATGTGGTCACCATGGTCGACGCGCTGTTGCGCTTGTCCg            >  4:16206/1‑136 (MQ=255)
                                                                                                                ccAACTGGGTTATCTGAATACCGACGCCTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGCCAGGTACCGGTGCATGATGTGGTCACCATGGTCGACGCGCGGTTTCGCTTGTCCGGGGc        >  2:154831/1‑135 (MQ=255)
                                                                                                                      gggTTATCTGAATACCGACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGCCAGGTACCGGTGCATGATGTGGTCACCATGGTCGACGCGCTGTTGCGCTTGTCCGGGGCttcgtt  >  6:84849/1‑135 (MQ=255)
                                                                                                                                  |                                                                                                                          
GCCAGAGTGGGCGCCCGGAGGTAGCGTTCGGCGCCTCAAAGTTCGCCCTCAACGGCATGGCTGATGCGCTGCGCGAAGGTTTCAGGGATGACAACCTGGCCGTGACCGTGCTCCAACTGGGTTATCTGAATACCGACGACTCCCTGAACACGCCATTGGCACAAGCCGCAGCAAGTGGCGAGCGGCGCCAGGTACCGGTGCATGATGTGGTCACCATGGTCGACGCGCTGTTGCGCTTGTCCGGGGCTTCGTT  >  ACB122/2847709‑2847961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑