Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 2,947,303 C→T 7.7% M84I (ATG→ATA)  puuP_2 ← Putrescine importer PuuP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,947,3030CT 7.7% 163.0 / 3.9 52M84I (ATG→ATA) puuP_2Putrescine importer PuuP
Reads supporting (aligned to +/- strand):  ref base C (24/24);  new base T (2/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

TCAGCAGATAGCTGAGCAGCGGCAGAAAGATGTAGTCGAGCAGCAGTGTCCAACCGGTGATGAAGCCGATATGGCTGCCGAAGGCCTTGCGCGTGTAGGTGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGAATACGGCCAGGGGCACCATGTAGGCCAG  >  ACB122/2947170‑2947437
                                                                                                                                     |                                                                                                                                      
tCAGCAGATAGCTGAGCAGCGGCAGAAAGATGTAGTCGAGCAGCAGTGTCCAACCGGTGATGAAGCCGATATGGCTGCCGAAGGCCTTGCGCGTGTAGGTGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACCa                                                                                                                                       <  6:152179/135‑1 (MQ=255)
tCAGCAGATAGCTGAGCAGCGGCAGAAAGAGGTAGTCGAGCAGCAGTGTCCAACCGGTGATGAAGCCGATATGGCTGCCGAAGGCCTTGCGCGGGTAGGGGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACCa                                                                                                                                       <  5:44779/135‑1 (MQ=255)
       aTAGCTGAGCAGCGGCAGAAATCTGTAGTCGAGCAGCAGTGTCCAACCGGTGATGAAGCCGATATGGCTGCCGAAGGCCTTGCGCGTGTAGGTGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACCATGCGGcc                                                                                                                                <  2:381322/135‑1 (MQ=255)
                 agcGGCAGAAAGATGTAGTCGAGCAGCAGTGTCCAACCGGTGATGAAGCCGATATGGCTGCCGAAGGCCTTGCGCGTGTAGGTGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTACCTGTAgg                                                                                                                     >  8:230148/1‑136 (MQ=255)
                      cAGAAAGATGTAGTCGAGCAGCAGTGTCCAACCGGTGATGAAGCCGATATGGCTGCCGAAGGCCTTGCGCGTGTAGGTGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCgg                                                                                                                  >  2:255261/1‑134 (MQ=255)
                        gAAAGATGTAGTCGAGCAGCAGTGTCCAACCGGTGATGAAGCCGATATGGCTGCCGAAGGCCTTGCGCGTGTAGGTGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTca                                                                                                               >  1:171567/1‑135 (MQ=255)
                                tAGTCGAGCAGCAGTGTCCAACCGGTGATGAAGCCGATATGGCTGCCGAAGGCCTTGCGCGTGTAGGTGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACTATGCGGCCGTAACTGTAGGCGGTCAGCAGCATgg                                                                                                      >  7:109372/1‑136 (MQ=255)
                                tAGTCGAGCAGCAGTGTCCAACCGGTGATGAAGCCGATATGGCTGCCGAAGGCCTTGCGCGTGTAGGTGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACTATGCGGCCGTAACTGTAGGCGGTCAGCAGCATgg                                                                                                      >  7:109371/1‑136 (MQ=255)
                                           ccgtggCCAAGCGGGGATGAAGCCGATATGGCTGCCGAAGGCCTTGCGCGTGTAGGTGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGt                                                                                            <  5:38026/133‑1 (MQ=255)
                                           cagTGTCCAACCGGTGATGAAGCCGATATGGCTGCCGAAGGCCTTGCGCGTGTCGGTGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGt                                                                                            <  5:33950/135‑1 (MQ=255)
                                                   aaCCTGTGATGAAGCCGATATGGCTGCCGAAGTCCTTGCGCGTGTAGGTGTAGACCGAGCCGTAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTGGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGt                                                                                    <  6:182451/135‑1 (MQ=255)
                                                   aaCCGGTGATGAAGCCGATATGGCTGCCGAAGGCCTTGCGCGTGTAGGTGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAg                                                                                      >  2:240649/1‑133 (MQ=255)
                                                        gTGATGAAGCCGATATGGCTGCCGAAGGCCTTGCGCGTGTAGGTGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGt                                                                                                                        >  3:222925/1‑94 (MQ=255)
                                                        gTGATGAAGCCGATATGGCTGCCGAAGGCCTTGCGCGTGTAGGTGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGt                                                                                                                        <  4:222925/94‑1 (MQ=255)
                                                              aaGCCTATATGGCTGCCGAAGGCCTTGCGCGTGTAGGTGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGc                                                                         <  4:30271/135‑1 (MQ=255)
                                                                       tGGCTGCCGAAGGCCTTGCGCGTGTAGGTGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCtt                                                               <  4:359331/136‑1 (MQ=255)
                                                                          atgacGAAGGCCTTGAGCGGGTTGGTGTAGACCGAGCCGGTGTAGGGGTGGGCCTGGACCATGAGGCAGTATCTGTAGGCGGTCAGCAGCATGGGGGCGAGCGTGAGCTGGTAGGCGGTGGGAAGGTGCCCTTTgg                                                            <  7:313221/132‑1 (MQ=255)
                                                                                aaGGCCTTGCGCGTGTAGGTGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCt                                                      >  7:185617/1‑136 (MQ=255)
                                                                                   gCCTTGCGCGTGTAGGTGTAGACCGAGCCGGAGTCGGGGTGGGCCTGGACTATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTggg                                                   <  8:109371/136‑1 (MQ=255)
                                                                                   gCCTTGCGCGTGTAGGTGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACTATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTggg                                                   <  8:109372/136‑1 (MQ=255)
                                                                                    ccTTGCGCGTGTAGGTGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGt                                                  <  3:221379/136‑1 (MQ=255)
                                                                                     cgtGCGCGTGTAGGTGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGGATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGt                                                  <  1:83409/133‑1 (MQ=255)
                                                                                     cTTGCGCGTGTAGGTGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGt                                                  <  2:171567/135‑1 (MQ=255)
                                                                                          gcgTGTAGGTGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACCATACGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCa                                             >  5:250354/1‑135 (MQ=255)
                                                                                             tgtaggtgtagCCCGAGCCGGAGTAGGGGTGGGCCGGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCGCTTTGGTCATCTGGGTGACCAGcc                                          <  1:254201/135‑1 (MQ=255)
                                                                                                         ccGAGCCGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGa                              <  1:255261/135‑1 (MQ=255)
                                                                                                          cGAGCCGGAGTAGGGGTGGGCCTGGACCATGCGGCGGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGa                              >  5:372015/1‑134 (MQ=255)
                                                                                                              ccGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGAATACgg                        >  3:257108/1‑136 (MQ=255)
                                                                                                               cGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGt                                                                                            >  3:368439/1‑67 (MQ=255)
                                                                                                               cGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGt                                                                                            <  4:368439/67‑1 (MQ=255)
                                                                                                               cGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAggt                                   <  6:243713/124‑1 (MQ=255)
                                                                                                               cGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAggt                                   >  5:243713/1‑124 (MQ=255)
                                                                                                               cGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGa                              <  4:227512/129‑1 (MQ=255)
                                                                                                               cGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGa                              >  3:227512/1‑129 (MQ=255)
                                                                                                               cGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGAATACgg                        >  6:252702/1‑135 (MQ=255)
                                                                                                               cGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGAATACgg                        >  5:190303/1‑135 (MQ=255)
                                                                                                               cGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGAATACgg                        >  5:395245/1‑135 (MQ=255)
                                                                                                               cGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGAATACgg                        >  1:177305/1‑135 (MQ=255)
                                                                                                               cGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGAATACGGc                       >  3:159584/1‑136 (MQ=255)
                                                                                                               cGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGAATACGGc                       >  3:189868/1‑136 (MQ=255)
                                                                                                               cGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGAATACGGc                       >  4:335074/1‑136 (MQ=255)
                                                                                                               cGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGAATACGGc                       >  3:235322/1‑136 (MQ=255)
                                                                                                               cGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGAGTGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGAATCCgt                        >  2:356842/1‑134 (MQ=255)
                                                                                                                ggAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGAATACGGc                       <  5:55530/135‑1 (MQ=255)
                                                                                                                     cggggTGGGCCGGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCTGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGAATACGGCCAggg                  <  1:150989/134‑1 (MQ=255)
                                                                                                                     aGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGAATACGGCCAggg                  <  1:61662/135‑1 (MQ=255)
                                                                                                                     aGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGGCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGAATACGGCCAggg                  <  5:259768/135‑1 (MQ=255)
                                                                                                                          tGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGAATACGGCCAGGGGCAcc             <  6:190303/135‑1 (MQ=255)
                                                                                                                               cTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGAATACGGCCAggg                  >  7:306647/1‑125 (MQ=255)
                                                                                                                               cTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGAATACGGCCAggg                  <  8:306647/125‑1 (MQ=255)
                                                                                                                               cTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGAATACGGCCAGGGGCACCATGTa        >  5:264720/1‑135 (MQ=255)
                                                                                                                               cTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGGAGGTGGTGAAGACGGCCAGGGGCACCATGTa        >  6:288013/1‑135 (MQ=255)
                                                                                                                                    ccATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGAATACGGCCAGGGGCACCATGTaggccag  >  8:204915/1‑136 (MQ=255)
                                                                                                                                     cATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGAGAGCGTGAGCAGGTAGGAGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGAAGACGGCACGGGGCACCATGTaggccag  >  1:287269/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                                      
TCAGCAGATAGCTGAGCAGCGGCAGAAAGATGTAGTCGAGCAGCAGTGTCCAACCGGTGATGAAGCCGATATGGCTGCCGAAGGCCTTGCGCGTGTAGGTGTAGACCGAGCCGGAGTAGGGGTGGGCCTGGACCATGCGGCCGTAACTGTAGGCGGTCAGCAGCATGGCGGCGAGCGTGAGCAGGTAGGCGGTGGGCAGGTGCCCTTTGGTCATCTGGGTGACCAGCCCGTAGGTGGTGAATACGGCCAGGGGCACCATGTAGGCCAG  >  ACB122/2947170‑2947437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑