Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	3,047,043	T→A	5.9%	A180A (GCA→GCT)	CJ019_02862 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	3,047,043	0	T	A	5.9%	216.1 / 4.1	68	A180A (GCA→GCT)	CJ019_02862	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (35/29);  new base A (2/2);  total (37/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01

GGATGGCTTCGCGAAACAGGGGGTGATCGTCGGCAATCAGAATTTTGTACATGGCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGT  >  ACB122/3046916‑3047173                                                                                                                                |                                                                                                                                   ggATGGCTTCGCGAAACAGGGGGTGATCGTCGGCAATCAGAATTTTGTACATGGCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCt                                                                                                                             >  5:79692/1‑135 (MQ=255) ggATGGCTTCGCGAAACAGGGGGTGATCGTCGGCAATCAGAATTTTGTACATGGCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTg                                                                                                                            >  3:134238/1‑136 (MQ=255)         tCGCGAAACAGGGGGTGATCGTCGGCAATCAGAATTTTGTACATGGACTTTTCACCTTGTTTTTATTGGAAGGAGCGGGGCACGCATACACAGGCAATTCAGGGAAACGGCACCGGTTCTGCCGTCTGCAATg                                                                                                                       >  2:75196/1‑133 (MQ=255)             gAAACAGGGGGTGATCGTCGGCAATCAGAATTTTGTACATGGCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGtt                                                                                                                >  7:284778/1‑136 (MQ=255)             gAAACAGGGGGTGATCGTCGGCAATCAGAATTTTGTACATGGCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGt                                                                                                                 >  1:149694/1‑135 (MQ=255)             gAAACAGGGGGTGATCGTCGGCAATCAGAATTTTGTACATGGCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGt                                                                                                                 >  2:100161/1‑135 (MQ=255)             gAAACAGGGGGTGATCGTCGGCAATCAGAATTTTGTACATGGCATTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTACAGGGAAACGTCACCGGTTGTGCCGGCTGCAATGGCAGGtt                                                                                                                >  3:255888/1‑136 (MQ=255)                   gggggTGATCGTCGGCAATCAGAATTTTGTACATGGCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCtt                                                                                                           <  5:138796/135‑1 (MQ=255)                       gggATCGGCGGCAATCAGAATTTTGTACATGGCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTtgctg                                                                                                       <  2:163265/133‑1 (MQ=255)                       gTGATCGTCGGCAATCAGAATTTTGTACATGGCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTtgctgc                                                                                                      >  8:258885/1‑136 (MQ=255)                       gTGATCGTCGGCAATCAGAATTTTGTACATGGCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTtgctg                                                                                                       <  1:361888/135‑1 (MQ=255)                            cgtcgGCAATCAGAATTTTGTACATGGCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGc                                                                                                 <  7:146244/136‑1 (MQ=255)                                       aGAATTTTTTACTTGGCGTTTTCACGTTGTTTTTATTGGATGGAGCGGGGGACGCATAGACAGGCACTTCAGGGAAACGTCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATGCAGGCCGt                                                                                      <  7:18174/136‑1 (MQ=255)                                        gAATTTTGTACATGGCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGTCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGt                                                                                      <  6:3267/135‑1 (MQ=255)                                           ttttGTACATGGCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTcgc                                                                                   <  5:362304/135‑1 (MQ=255)                                              tGTACATGGCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCgg                                                                                 <  6:240381/134‑1 (MQ=255)                                                  cATGGCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCa                                                                            <  7:367762/135‑1 (MQ=255)                                                  cATGGCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCa                                                                            <  3:34667/135‑1 (MQ=255)                                                     ggCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTAc                                                                        <  4:3067/136‑1 (MQ=255)                                                      gCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGt                                                                          >  5:326834/1‑133 (MQ=255)                                                      gCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTAc                                                                        <  2:190716/135‑1 (MQ=255)                                                      gCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTAc                                                                        <  4:256428/135‑1 (MQ=255)                                                      gCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTAc                                                                        <  4:11806/135‑1 (MQ=255)                                                      gCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTAc                                                                        <  7:65400/135‑1 (MQ=255)                                                      gCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACa                                                                       <  4:203187/136‑1 (MQ=255)                                                       ccTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTAc                                                                        <  1:100161/134‑1 (MQ=255)                                                       ccTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTAc                                                                        <  1:28626/134‑1 (MQ=255)                                                        cTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGcc                                                                                                     >  1:344068/1‑104 (MQ=255)                                                        cTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGcc                                                                                                     <  2:344068/104‑1 (MQ=255)                                                            tCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGgttg                                                                  <  8:313810/135‑1 (MQ=255)                                                             cACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGgttgt                                                                 >  3:285791/1‑135 (MQ=255)                                                                    tttttATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGcc                                                          >  3:274648/1‑135 (MQ=255)                                                                     ttttATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCa                                                                            >  7:299483/1‑116 (MQ=255)                                                                     ttttATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCa                                                                            <  8:299483/116‑1 (MQ=255)                                                                              atggaGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCttt                                                 <  1:64503/134‑1 (MQ=255)                                                                                 gaGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTcgcgc                                            >  4:13855/1‑136 (MQ=255)                                                                                          aCGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGt                                                                          <  6:83800/97‑1 (MQ=255)                                                                                          aCGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGt                                                                          >  5:83800/1‑97 (MQ=255)                                                                                          aCGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACcg                                   >  7:11337/1‑136 (MQ=255)                                                                                            gCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACcgc                                  <  1:304256/135‑1 (MQ=255)                                                                                              aTACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGt                                >  5:234770/1‑135 (MQ=255)                                                                                               tACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCa                                                                            <  1:228467/90‑1 (MQ=255)                                                                                               tACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCa                                                                            >  2:228467/1‑90 (MQ=255)                                                                                               tACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTc                              >  8:206168/1‑136 (MQ=255)                                                                                               tACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTc                              >  3:156153/1‑136 (MQ=255)                                                                                                  acaGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAg                           <  3:13855/136‑1 (MQ=255)                                                                                                    aGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGc                          >  6:42551/1‑135 (MQ=255)                                                                                                    aGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCt                         >  4:222807/1‑136 (MQ=255)                                                                                                    aGGCACTTCAGGGAAACGGCACCGGTTGAGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGt                                                                          >  5:44856/1‑87 (MQ=255)                                                                                                    aGGCACTTCAGGGAAACGGCACCGGTTGAGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGt                                                                          >  5:39614/1‑87 (MQ=255)                                                                                                    aGGCACTTCAGGGAAACGGCACCGGTTGAGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGt                                                                          <  6:39614/87‑1 (MQ=255)                                                                                                    aGGCACTTCAGGGAAACGGCACCGGTTGAGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGt                                                                          <  6:44856/87‑1 (MQ=255)                                                                                                         cTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTaa                      >  5:170945/1‑134 (MQ=255)                                                                                                         cTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTaa                      >  2:237047/1‑134 (MQ=255)                                                                                                         cTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTaa                      >  6:263560/1‑134 (MQ=255)                                                                                                         cTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAAc                     >  8:118241/1‑135 (MQ=255)                                                                                                         cTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAAc                     >  6:18199/1‑135 (MQ=255)                                                                                                         cTTCAGGGAAACGCCACCGGTTGTGCCGCCTGCAATGCCACGTTGGCTAGCTCCCACGCATCCACGCCGTCGCGGTACCAGAACAGGATGTAATACCCCAAGCCTTTCGCGCGATATACCGCGTTCCAGCTTaa                      >  1:89826/1‑134 (MQ=255)                                                                                                           tCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAg                  >  4:162236/1‑136 (MQ=255)                                                                                                             aGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATGGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGc                 >  3:24422/1‑135 (MQ=255)                                                                                                             aGGGAAACGGCACAGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCACGGCTTTCGCGCGTTATACCGCGATCCAGCTTAACCCGc                 >  7:387222/1‑135 (MQ=255)                                                                                                                     ggCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAg         <  5:384614/135‑1 (MQ=255)                                                                                                                      gCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGc        <  2:287695/135‑1 (MQ=255)                                                                                                                       cACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCg       >  6:176284/1‑135 (MQ=255)                                                                                                                       cACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGATGAACCGCGTTCCAGCTTAACCAGCAATCGGAGCgg      >  7:367313/1‑136 (MQ=255)                                                                                                                       cACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCAGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCgg      >  4:157763/1‑136 (MQ=255)                                                                                                                          cGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAg   <  4:134238/136‑1 (MQ=255)                                                                                                                            gTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGt  >  3:272675/1‑135 (MQ=255)                                                                                                                                |                                                                                                                                   GGATGGCTTCGCGAAACAGGGGGTGATCGTCGGCAATCAGAATTTTGTACATGGCCTTTTCACCTTGTTTTTATTGGATGGAGCGGGGCACGCATACACAGGCACTTCAGGGAAACGGCACCGGTTGTGCCGGCTGCAATGGCAGGTTGGCTTGCTGCCAGGCATCCAGGCCGTCGCGGTACCAGTACAGGTTGTTATAGCCCAAGGCTTTCGCGCGTTTTACCGCGTTCCAGCTTAACCAGCAATCGGAGCGGCAGT  >  ACB122/3046916‑3047173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑