| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 3,119,656 | A→C | 18.3% | F276V (TTC→GTC) | prs2 ← | Putative ribose‑phosphate pyrophosphokinase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,119,656 | 0 | A | C | 18.3% | 102.3 / 2.1 | 60 | F276V (TTC→GTC) | prs2 | Putative ribose‑phosphate pyrophosphokinase 2 |
| Reads supporting (aligned to +/- strand): ref base A (19/28); new base C (2/11); total (21/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCATGCGCCAGGGCCCGAACGGTTGGCAAGCAGCTCGGCGACCAGGGCGCTGGTATCGACGATATCCAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCTGGGCAGCGCGTAGCAGCGTCTGCCCCGTGCTGATCAGGTCGT > ACB122/3119522‑3119790 | tCATGCGCCAGGGCCCGAACGGTTGGCAAGCAGCTCGGCGACCAGGGCGCTGGTATCGACGATGTCCAGTTGCTCCAGGCATCGCACAGGAAGGCGAAAGGGGGCGATGCTGTCGGGCCGCAGTACCCGTTCGaa > 1:161274/1‑135 (MQ=255)tCATGCGCCAGGGCCCGAACGGTTGGCAAGCAGCTCGGCGACCAGGGCGCTGGTATCGACGATATCCAGTTGCTCCAGGCATCGCCCAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAAc > 8:80839/1‑136 (MQ=255)tCATGCGCCAGGGCCCGAACGGTTGGCAAGCAGCTCGGCGACCAGGGCGCTGGTATCGACGATATCCAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAAc > 4:336798/1‑136 (MQ=255) ccggggcCAGGCCACGACCGGGTGGCAAGCAGCGCGGCGACCCGGGCGCTGGGACCGACGATATCCAGTTGCGCCAGGCTTCGCACAGGCAGGCGAAAGGTGGCGATGCTGTCGGCCACCAGTACCCGTTCGAAcg < 7:168517/131‑1 (MQ=255) tGCGCCAGGGCCCGAACGGTTGGCAAGCAGCTCGGCGACCAGGGCGCTGGTATCGACGATATCCAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAAcgcg < 3:188162/136‑1 (MQ=255) tGCGCCAGGGCCCGAACGGTTGGCAAGCAGCTCGGCGACCAGGGCGCTGGTAGCGACGATATCCAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAAcgcg < 3:157232/136‑1 (MQ=255) gcgcCAGGGCCCGAACGGTTGGCAAGCAGCTCGGCGACCAGGGCGCTGGTATCGACGATATCCAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAAcgcg < 6:269206/135‑1 (MQ=255) cgcCAGGGCCCGAACGGTTGGCAAGCAGCTCGGCGACCAGGGCGCTGGTATCGACGATATCCAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAACGcgcc > 3:149195/1‑136 (MQ=255) ccAGGGCCCGAACGGTTGGCAAGCAGCTCGGCGACCAGGGCGCTGGTATCGACGATATCCAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGACCGcgccgc > 4:15969/1‑136 (MQ=255) ggCCCGAACGGTTGGCAAGCAGCTCGGCGACCAGGGCGCTGGTATCGACGATATCCAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGt > 6:115780/1‑135 (MQ=255) gCCCGAACGGTGGGCAAGCAGCTCGGCGACCAGGGCGCTGGTATCGACGATATCCAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTc < 6:152995/135‑1 (MQ=255) ccGAACGGTTGGCAAGCAGCTCGGCGACCAGGGCGCTGGTATCGACGATATCCAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGa > 5:102700/1‑135 (MQ=255) cGGTTGGCAAGCAGCTCGGCGACCAGGGCGCTGGTATCGACGATATCCAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGc > 5:269540/1‑135 (MQ=255) gCATGCAGCGCGGCGACCTGGGCGGGGGTATCGACGATATCCAGTTGCTCCAGGCTGCGCACAGGCAGGGGAACGGGGGCGATGCGTTCGGCCCCCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCa < 6:102700/135‑1 (MQ=255) gCAAGCAGCTCGGCGACCAGGGCGCTGGTATCGACGATATCCAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCa > 1:382260/1‑135 (MQ=255) gcTCGGCGACCAGGGCGCTGGTATCGACGATATCCAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCggtggt < 8:322666/136‑1 (MQ=255) cTCGGCGACCAGGGCGCTGGTATCGACGATATCCAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCTATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCggtggt < 6:120408/135‑1 (MQ=255) cTCGGCGACCAGGGCGCTGGTATCGACGATATCCAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCggtggt < 6:114866/135‑1 (MQ=255) atacgCGACGGGGGCGCTGGTAGCTACTATATCCAGTTGCTCCCGGCAGCCCACCGGCAGGCGAACGGGGGCGATGCTGGGGGCCACCTGTACCCGTTCGACCGCGCCGCTGTCGAACAGCTCGCCACCggtggt < 4:15701/131‑1 (MQ=255) cGGCGACCAGGGCGCTGGTATCGACGATATCCAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCGgtggtgc > 2:164638/1‑134 (MQ=255) cGGCGACCAGGGCGCTGGTATCGACGATATCCAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGa > 5:244091/1‑135 (MQ=255) gggCGCTGGTATCGACGATATCCAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCgt > 6:383608/1‑135 (MQ=255) aTCGACAATATCCCGTTGCGCCAGGCATCGCACAGGCAGGCGAACGGGGGCGAGGCTGGCGGCCACCAGTACCCGTGCGACCGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCcgc < 5:308710/135‑1 (MQ=255) gaTATCCAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAg < 8:94518/114‑1 (MQ=255) gaTATCCAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAg > 7:94518/1‑114 (MQ=255) ccAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGCACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCCGCATGCg > 7:80605/1‑136 (MQ=255) ccAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGACCGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACCGGCCGTGGGTGGCCGCGGCCCGCATGCg > 7:340056/1‑136 (MQ=255) ccAGTTGCGCCAGGCATCGCACAGGCAGGCCAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCGGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGGGGCCGCGGCCAGCATGc < 1:206160/135‑1 (MQ=255) cTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTCGCCGCGGCCAGCATGCggctggc > 5:257152/1‑135 (MQ=255) cTCCAGCCAGCGCACAGGCCGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTCCGAACCCGCCGCTGGCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGGGGCCGCGGCCAGCATGCggctggct < 3:138479/136‑1 (MQ=255) aGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGGCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCTcc < 2:97869/134‑1 (MQ=255) aGGCAGCGCACCGGCAGGCGAACGGGGGCGATGCTGTCGGCCACCAGTACCCGTGCGACCGCGCCGCTGGCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGGGGCCGCGGCCAGCATGCGGCTGGCTCCgg < 3:97920/136‑1 (MQ=255) cgccccggccggcGAATGGGGGGGTGGCTGGCGGCCACCAGTCCCCGTAGCACCGCGCCGCGGTCGAACAGCGCGCCACCGGTGGTGAACAGGCCGTGGGGGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGc < 5:207117/129‑1 (MQ=255) cGCACAGGCAGGCGAAAGGGGGCGATGCTGGCGGCCACCAGTCCCCGTTCGACCGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGc < 6:319649/135‑1 (MQ=255) ggcGAACGGGGGCGAGGCGGTCGCCCACCAGAACCCGGACGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCt < 5:93152/135‑1 (MQ=255) ggcGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTCGCCGCGGCCAGCATGCGGCTGGCGCCGGCCTGCCGGCAGGCCt < 6:257152/135‑1 (MQ=255) gcGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCTgg < 7:126027/136‑1 (MQ=255) cGAACGGGGGCGATGCTGTCGGCCCCCCGACCCCGTGCGACCGCGCCGCGGGCGAACAGCTCGCCACCGGTGGGGAACAGGCCGTGGGGGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCTgg < 2:90891/135‑1 (MQ=255) gAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCACGCCTggg > 2:215336/1‑135 (MQ=255) ggCGATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCTGGGCAGcgcg > 8:286707/1‑135 (MQ=255) ggCAATGCTGGCGGCCACCAGTACCCGTGCGACCGCGCCGCTGGCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGGGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCTGGGCAGCGCGt < 8:90452/136‑1 (MQ=255) gCGATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCTGGGCAGCGCGt < 2:339161/135‑1 (MQ=255) gCGAGGCTGTCGGCCACCAGTACCCGTGCGACCGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGGGGGTGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCTGGGCAGCGCGt < 6:348035/135‑1 (MQ=255) aTGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCGCCGGCCAGCCCGCACGCCTGGGGAGCGCGTagca > 3:100775/1‑136 (MQ=255) tGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCTGGGCAGCGCGTagcag < 4:294653/136‑1 (MQ=255) gCTGTCGGCCCCCAGTACCCGTGCGAACGCGCCGCTGTCGAACAGCGCGCCACCGGTGGTGAACAGGCCGTGGGGGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCTGGGCAGCGCGTagcag < 1:283495/135‑1 (MQ=255) gCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCTGGGCAGCGCGTagcag < 4:149195/135‑1 (MQ=255) cTGTCGGCCACCAGTCCCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGACCAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCTCCGTCCTGCCGGCAGGCCTGGGCAGCTCGTagcag < 8:195985/134‑1 (MQ=255) tCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCTGGGCAGCGCATAGCAGCGTCt < 8:308321/136‑1 (MQ=255) acacagaccaGTACCCGTTCGACCGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCTGGGCAGCGCGTAGCAGCGTCt < 7:147493/130‑1 (MQ=255) cgggccccaGTACCCGTTCGACCGCGCCCGTGGCGAACAGCGCGGCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGGATGGCGCTGGCGCCGGCCAGCCGGCATGCCTGGGCAGCGCGTAGCAGCGTCt < 5:161912/129‑1 (MQ=255) cGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCTGGGCAGCGCGTAGCAGCGTCt < 6:269540/135‑1 (MQ=255) cGGCCACAAGAACCCGTTCCACCGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCTGGGCAGCGCGTAGCAGCGTCt < 6:370571/135‑1 (MQ=255) gCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGACGTGGGTGGCCGCGGCCAGCAAGCGGCTGGCTCCGGCCTGCAGGCAGGCCGTGGCAGCGCGGAGCAGCCGCTGc > 5:58989/1‑135 (MQ=255) aaccaGTACCCGTGCGAACGCGCCGCTGTCGAACTGCGCGCCTCCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCTGGGCAGCGCGTAGCAGCGTCTGcccc < 3:15969/135‑1 (MQ=255) accaGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCTGGGCAGCGCGTAGCAGCGTCTGcccc < 6:244091/135‑1 (MQ=255) accaGTACCCGTCCGAACGCGCCGCTGGCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGGGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCTGGGCAGCGCGTAGCAGCGTCTGcccc < 1:153855/135‑1 (MQ=255) gTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCCGCATGCGGCTGGCTCCGGCCGGCCCGCAGGCCAGGGCCCGCCCTAGCAGCGGGTGGCCCGTggg > 7:205977/1‑134 (MQ=255) tCGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCTGGGCAGCGCGTAGCAGCGTCTGCCCCGTGCTGATCAgg < 2:129365/135‑1 (MQ=255) cGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCTGGGCAGCGCGTAGCAGCGTCTGCCCCGTGCTGATCAGgt < 7:285338/135‑1 (MQ=255) cGAACGCGCCGCTGTCGAACAGCACGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCTGGGCAGCGCGTACCCGCGTCTGCCCCGTGCTGATCAGctc > 8:289524/1‑136 (MQ=255) aaCGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCTGGGCAGCGCGTAGCAGCGTCTGCCCCGTGCTGATCAGgtcgt > 7:332869/1‑136 (MQ=255) | TCATGCGCCAGGGCCCGAACGGTTGGCAAGCAGCTCGGCGACCAGGGCGCTGGTATCGACGATATCCAGTTGCTCCAGGCATCGCACAGGCAGGCGAAAGGGGGCGATGCTGTCGGCCACCAGTACCCGTTCGAACGCGCCGCTGTCGAACAGCTCGCCACCGGTGGTGAACAGGCCGTGGGTGGCCGCGGCCAGCATGCGGCTGGCTCCGGCCTGCCGGCAGGCCTGGGCAGCGCGTAGCAGCGTCTGCCCCGTGCTGATCAGGTCGT > ACB122/3119522‑3119790 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |