| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 189,506 | T→C | 15.7% | T1455T (ACT→ACC) | CJ019_00185 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 189,506 | 0 | T | C | 15.7% | 45.8 / 3.9 | 27 | T1455T (ACT→ACC) | CJ019_00185 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (15/6); new base C (2/2); total (19/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
AGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGT > ACB122/189374‑189640 | agcgACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCTGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCTTCACCATCGAGAACGCCACTgg < 1:304743/132‑1 (MQ=12) aaCGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACAGTCAGCGTCACCATCGAGATCGCCACGGGCGGCACATTCGag > 4:136657/1‑134 (MQ=11) ccAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGc > 5:147917/1‑134 (MQ=255) ccAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTg > 7:128262/1‑136 (MQ=255) ccAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGCAACtt < 4:207581/126‑1 (MQ=25) ccAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGCAACtt > 3:207581/1‑126 (MQ=25) ccATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGAccc > 1:146637/1‑135 (MQ=255) ccATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGCAACTTCGAACa < 2:126574/127‑1 (MQ=38) ccATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGCAACTTCGAACa > 1:126574/1‑127 (MQ=38) tACCGTTGAAGCCGGCAAAACCCAGGGCTGCGTCGATTCCCAGACCCCGGCGAAGGACTTCTACAACAACGGTTCGACGGTCAGGGTCACCATCGACAACGCCACTGGCGGAAACTTCGAACACCTGACCCCGaa < 2:41929/135‑1 (MQ=11) gTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCTAGAACGCCACTTGCGTCAACTTCGAACAGCTGACCCCGAACCCGa < 8:113370/136‑1 (MQ=255) aaGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGcc > 4:379542/1‑136 (MQ=37) gCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCg > 2:316848/1‑135 (MQ=37) gCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGCAAATTCGAACAGCTGACCCCGAAGCCGACCCCgg > 8:180579/1‑136 (MQ=255) ggCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTc < 8:30103/136‑1 (MQ=37) cAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCATCt < 5:105819/89‑1 (MQ=37) cGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAAGTTCGAGCAGCTGACCCCGAACCCGACCCCGGCTCAGACCACGAGCAACGACTCg > 8:331248/1‑136 (MQ=255) cGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCg > 3:15475/1‑136 (MQ=34) gaccggACCCCGGCGAATGACGTCTACAACACCGGTTCGACCGGCAGCGGCACCATCGAGAGCGCCACTGGGGGAAAATTCGAACAGCGGCCCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGt < 8:290129/129‑1 (MQ=11) tCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGCCCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGa > 7:285887/1‑136 (MQ=21) tCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGa > 7:291648/1‑136 (MQ=34) gAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCCCCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACcgcg > 7:387878/1‑136 (MQ=18) gTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGCCCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTg > 1:336582/1‑135 (MQ=17) cGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGCCCCTGACGGCGAGccc > 5:48488/1‑135 (MQ=17) ttCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTc > 6:386194/1‑135 (MQ=32) ccGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCa > 5:296357/1‑135 (MQ=32) cACTGGCGGCAACTTCGAACAGCTGCCCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCTCCGAAGGCGGCGTGATCCCCTACAcc > 8:23256/1‑136 (MQ=12) tGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCCCCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGt > 1:148825/1‑135 (MQ=11) | AGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGT > ACB122/189374‑189640 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |