Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,228,172 A→G 5.1% G70G (GGA→GGG)  garD_1 → Galactarate dehydratase (L‑threo‑forming)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,228,1720AG 5.1% 251.5 / 2.2 77G70G (GGA→GGG) garD_1Galactarate dehydratase (L‑threo‑forming)
Reads supporting (aligned to +/- strand):  ref base A (25/48);  new base G (2/2);  total (27/50)
Fisher's exact test for biased strand distribution p-value = 6.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

CCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAGCGCCGCGCAGTTCCAGG  >  ACB122/3228043‑3228302
                                                                                                                                 |                                                                                                                                  
ccTGCTGCCAGGCGTGCGCCTGGATGACGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACGGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCg                                                                                                                              <  3:297596/136‑1 (MQ=255)
     tgcCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTcc                                                                                                                           >  4:115349/1‑134 (MQ=255)
      gcCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCCGCCCCTCCCTTCAGGCCACAAGCTCGCCCCCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGAGCATCGGATTTGCGTCCCa                                                                                                                         >  1:377066/1‑135 (MQ=255)
          ggCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGa                                                                                                                    <  3:175255/136‑1 (MQ=255)
               gcgcCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGAc                                                                                                               >  4:21633/1‑136 (MQ=255)
                 gcCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGc                                                                                                             >  3:31046/1‑136 (MQ=255)
                 gcCTGGAAGCCGAGGCGATTACCGTGCGCCAGCCCCTCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGc                                                                                                             <  7:108805/136‑1 (MQ=255)
                  ccTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCt                                                                                                            <  7:326461/136‑1 (MQ=255)
                   cTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTGAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCt                                                                                                            <  5:76099/135‑1 (MQ=255)
                   cTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCt                                                                                                            >  1:220958/1‑135 (MQ=255)
                       aTGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTgg                                                                                                          <  1:153249/133‑1 (MQ=255)
                        tGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGGTTTGCGTCCCAGGCGATCGACGCTGGCGAc                                                                                                      >  3:83547/1‑136 (MQ=255)
                        tGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGa                                                                                                       >  5:60025/1‑135 (MQ=255)
                        tGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGa                                                                                                       >  5:300566/1‑135 (MQ=255)
                        tGCCGAGGCGATTACCGTGAGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGAGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGGTTTGCGTCCCAGGCGATCGACGCTGGCGAc                                                                                                      >  3:83545/1‑136 (MQ=255)
                          ccGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGAcc                                                                                                     <  8:63659/135‑1 (MQ=255)
                          ccGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTAGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGAcc                                                                                                     <  7:373093/135‑1 (MQ=255)
                           cGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACcat                                                                                                   <  3:251301/136‑1 (MQ=255)
                            gAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACcat                                                                                                   <  5:244543/135‑1 (MQ=255)
                             aGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACcatgt                                                                                                 <  4:63876/136‑1 (MQ=255)
                             aGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACcatg                                                                                                  <  5:373714/135‑1 (MQ=255)
                             aGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCCGGCGACcatgt                                                                                                 <  4:311755/136‑1 (MQ=255)
                              ggCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACcatgt                                                                                                 <  1:290978/135‑1 (MQ=255)
                              ggCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACcatgt                                                                                                 >  1:144438/1‑135 (MQ=255)
                                cGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACcatgtgc                                                                                               >  2:343865/1‑135 (MQ=255)
                                  aTTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACcatgtgcatg                                                                                            <  4:120375/136‑1 (MQ=255)
                                    tACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACcatgtgcatgt                                                                                           <  5:76805/135‑1 (MQ=255)
                                       cGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCatgtgcatgtgc                                                                                         <  6:64519/134‑1 (MQ=255)
                                            gcCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCg                                                                                                        >  3:103626/1‑114 (MQ=255)
                                            gcCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCg                                                                                                        <  4:103626/114‑1 (MQ=255)
                                              cAGCCCATCCCTTCGGGCCACAAGCTCGCCACCGAGCCTGTCGAGCAAGGCCAGCCGTTGCGCCGATACGGGAAGATGATCGGATTTGCGTCCCAGGCGATCGCCGCGGGCGCCCATGTGCATGTGCACACCGTg                                                                                 >  5:114457/1‑135 (MQ=255)
                                              cAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGt                                                                                  >  6:163741/1‑134 (MQ=255)
                                              cAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATAGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGt                                                                                  >  1:137954/1‑134 (MQ=255)
                                                   cATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGAt                                                                            <  1:364450/135‑1 (MQ=255)
                                                        cTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGAcc                                                                                                     >  1:84520/1‑105 (MQ=255)
                                                        cTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGAcc                                                                                                     <  2:84520/105‑1 (MQ=255)
                                                        cTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCg                                                                       >  6:330974/1‑135 (MQ=255)
                                                         ttCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATCTGCACAACGTGCAGATGGGCGa                                                                      >  2:70792/1‑135 (MQ=255)
                                                             ggCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTg                                                                 <  4:352115/136‑1 (MQ=255)
                                                               ccACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGc                                                                <  2:84743/135‑1 (MQ=255)
                                                                 acaAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCatgtgcatgtgc                                                                                         <  1:163006/108‑1 (MQ=255)
                                                                 acaAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCatgtgcatgtgc                                                                                         >  2:163006/1‑108 (MQ=255)
                                                                   aaGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCcgc                                                            <  5:354785/135‑1 (MQ=255)
                                                                           cACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGc                                                    >  1:88182/1‑135 (MQ=255)
                                                                           cACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGc                                                    <  1:343865/135‑1 (MQ=255)
                                                                           cACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCCTATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGc                                                    >  1:234824/1‑135 (MQ=255)
                                                                            aCCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGt                                                  <  4:306769/136‑1 (MQ=255)
                                                                             ccGAGCCTGTGGCGCAAGGCCTGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGt                                                  <  2:197021/135‑1 (MQ=255)
                                                                             ccGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGCCTATGCGtt                                                 >  8:323665/1‑136 (MQ=255)
                                                                             ccGAGCCGGTGGCGCAAGGCCCGCCGGTGCGCCGTTACGGGCAGATCATCGGATTTGCGGCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGAGTTTGCCCGCGACTATGCGt                                                  <  6:371784/135‑1 (MQ=255)
                                                                                   cTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGgtg                                            <  2:220958/135‑1 (MQ=255)
                                                                                   cTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTATGCCCGCGACTATGCGTTTGgtg                                            >  1:386573/1‑135 (MQ=255)
                                                                                    tgtgGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGgtgt                                           <  1:273152/135‑1 (MQ=255)
                                                                                        gcgcAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGAt                                       >  6:254699/1‑135 (MQ=255)
                                                                                            aaGTCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATAccc                                   <  2:179642/135‑1 (MQ=255)
                                                                                                             cGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAgcg                 <  3:21633/136‑1 (MQ=255)
                                                                                                               ttACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAGcgcc               <  4:343959/136‑1 (MQ=255)
                                                                                                               ttACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAGcgcc               <  3:286837/136‑1 (MQ=255)
                                                                                                               ttACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACAATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAGcgcc               <  7:154540/136‑1 (MQ=255)
                                                                                                               ttACGGGCAGAGCATCGGAGTTGCGGCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACGATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAGcgcc               <  3:316386/136‑1 (MQ=255)
                                                                                                                tACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAGcgcc               <  2:88182/135‑1 (MQ=255)
                                                                                                                tACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAGcgcc               <  6:116432/135‑1 (MQ=255)
                                                                                                                tACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATCTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAGcgcc               <  1:123894/135‑1 (MQ=255)
                                                                                                                tACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATCTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAGcgcc               <  1:70792/135‑1 (MQ=255)
                                                                                                                tACGGGCAGATCATCGGAGTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAGcgcc               <  2:285769/135‑1 (MQ=255)
                                                                                                                tACGGGCAGAGCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAGcgcc               <  5:201463/135‑1 (MQ=255)
                                                                                                                  cGGGCAGATCATCGGGTTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAGCGCcgcg            <  4:83547/136‑1 (MQ=255)
                                                                                                                  cGGGCAGATCATCGGGTTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAGCGCcgcg            <  4:83545/136‑1 (MQ=255)
                                                                                                                  cGGGCAGATCATCGCATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAGcgccg              <  5:23659/134‑1 (MQ=255)
                                                                                                                   gggatgATCATCGGATTTGCGGCCCCGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGAGGGGCGATGTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAGCGCcgcg            <  1:379525/130‑1 (MQ=255)
                                                                                                                   gggCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCAGGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAGCGCcgcg            <  6:60025/135‑1 (MQ=255)
                                                                                                                       aGATCTTCGGATTTGCGTCCCTGGCGATCGACGCTGGCGACCATGTGCATGTGCTCAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAGCGCCGCGCAGtt       <  3:263288/136‑1 (MQ=255)
                                                                                                                       aGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAGCGCCGCGCAGt        <  6:156291/135‑1 (MQ=255)
                                                                                                                       aGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCAGGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGAGTCGATACCCGCAGCCTGCCAGGCAGCGCCGCGCAGt        <  5:229943/135‑1 (MQ=255)
                                                                                                                            atcGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCTTGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAGCGCCGCGCAGTTCCAgg  >  7:384977/1‑136 (MQ=255)
                                                                                                                            atcGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAGCGCCGCGCAGTTCCAgg  >  8:211366/1‑136 (MQ=255)
                                                                                                                            atcGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAGCGCCGCGCAGTTCCAg   >  2:242393/1‑135 (MQ=255)
                                                                                                                                 |                                                                                                                                  
CCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAGGCAGCGCCGCGCAGTTCCAGG  >  ACB122/3228043‑3228302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑