Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,267,810 T→G 27.7% L171V (TTG→GTG)  eamA → putative amino‑acid metabolite efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,267,8100TG27.7% 47.0 / 3.5 43L171V (TTG→GTG) eamAputative amino‑acid metabolite efflux pump
Reads supporting (aligned to +/- strand):  ref base T (4/26);  new base G (11/2);  total (15/28)
Fisher's exact test for biased strand distribution p-value = 1.45e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCACTGATCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGCCC  >  ACB122/3267680‑3267930
                                                                                                                                  |                                                                                                                        
ggaagtGATCGGCAGCGTAGAGGGCGGCGATGGGCCCATGGTGGCGTTGGTGCGGACGCTGTGTGGCGGGGGGATGTGGGGCGTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGt                                                                                                                      <  5:308809/130‑1 (MQ=255)
 gCACTGATCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGt                                                                                                                      <  3:307817/134‑1 (MQ=255)
       aTCGGCAGCGATGACGGCGGCCATGTGCCCTTGCGGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGGGGGGCGTGGGCAACATAATTACCCGACGGTTCGGCTCGGGCGACCTGGTCGCGTTTGTGATCTg                                                                                                                <  8:160317/134‑1 (MQ=255)
        tCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTg                                                                                                                <  1:337560/133‑1 (MQ=255)
          ggCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGCCCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGTTCTGGGGt                                                                                                            >  6:135073/1‑135 (MQ=255)
          ggCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGGTGTGGGGt                                                                                                            >  1:2967/1‑135 (MQ=255)
          ggCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGGGGTGGTGTggggg                                                                                                            >  6:358100/1‑134 (MQ=255)
               cGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGCCCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCACCATCATTACCCGACGGTTCGGCTCGGTCGACCGGGTCGCGGGGGGGGGGTGGGGTGGGcg                                                                                                       >  1:347684/1‑134 (MQ=255)
               cGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGTTCGGGGGTGGGCTg                                                                                                      >  4:305101/1‑136 (MQ=255)
                   gACGGCGGCCATGGGCCCTTGCTGGCGTTGGTGCTGACCCGGGGTGGCGGGGCGAGGGGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATAc                                                                                                  <  8:167566/136‑1 (MQ=255)
                         ggcCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGGGATGTGGGGTGGGGTGGTAACGccc                                                                                             >  7:94727/1‑134 (MQ=255)
                          gcCATGTGCCCATGCTGGCGTGGGTGCTGACCCTGTGTGGCGGGGGGATGTGGGGCCTGGGCAACATCATAACCCGACGGTGCGGCTCGGTCGACGTGGTCGCGTTGGTGAGCTGGGGGGGGCTGATACCGCCACt                                                                                           <  4:352145/136‑1 (MQ=255)
                                 gCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGGGGTCTGGGGGGGGCGGCTACCCCCCCTTCCAtac                                                                                    >  3:180269/1‑134 (MQ=255)
                                   ccATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGCTCGCGGTGGTGGGCTGGGGTGGGGGGATACCCCCCCATCCATTccg                                                                                  >  4:132256/1‑135 (MQ=255)
                                    cATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTg                                                                                 <  3:183215/136‑1 (MQ=255)
                                     aTGCTGGGGTGGGTGCTGACCCTGTGTGGCGGTGCGAGGGGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTg                                                                                 <  2:200560/135‑1 (MQ=255)
                                     aTGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGTGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTg                                                                                 <  6:317046/135‑1 (MQ=255)
                                               tGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTg                                                                                                                <  7:28502/94‑1 (MQ=255)
                                               tGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTg                                                                                                                >  8:28502/1‑94 (MQ=255)
                                                 gtgaggACCCTGTGTGGCGGGGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCtgg                                                                     <  5:21621/130‑1 (MQ=255)
                                                          cTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGCTTTGGGGTGGGGGGGTTCCGGCACTTCCATTACGGGCCATGTCCTGGTTGCgggta                                                            >  6:312930/1‑133 (MQ=255)
                                                              gtgGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGCTCTTGGGTGGGGTGATACCGCCACTTCCATTCCTGGGACTGGACTGGTGGCTGGAAGGccc                                                       >  8:194340/1‑136 (MQ=255)
                                                               tgGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGCTCTGGGGTGGGCTGATACCGCCACTTACCTTACAGGCACGGGCCGGGTGGCGGGAAGGccc                                                       >  2:269832/1‑135 (MQ=255)
                                                                   ggTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAgc                                                  <  3:352179/136‑1 (MQ=255)
                                                                         aTGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTg                                            <  4:171310/136‑1 (MQ=255)
                                                                          tgtgGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATAc                                                                                                  <  8:42316/81‑1 (MQ=255)
                                                                          tgtgGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTg                                            <  5:312930/135‑1 (MQ=255)
                                                                          tgtgGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGATCTGGGGTGGGCTGATAc                                                                                                  >  7:42316/1‑81 (MQ=255)
                                                                               ggCCTGGGCAACATCATTACCCGACGGGTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCAt                                       <  5:358100/135‑1 (MQ=255)
                                                                                    gggCACAATCATTACCCGACGGTTCGGCTCGGTCGTCGTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCtt                                  <  5:34686/135‑1 (MQ=255)
                                                                                             catTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGGTGTGGGGTGGGCGGGTTCCGCCCCCTACAATACAGGGACAGTCCTGGTGGCGGGAAGGCCCCCCGCCCAATTGCCCAGCCGTGGCCaaaaa                         >  8:367442/1‑132 (MQ=255)
                                                                                                     gACGGGTCGGCGCGGGCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGa                 <  6:155610/135‑1 (MQ=255)
                                                                                                         gTTCGGCTCGGTCGACCTGGTCGCGGTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCg            <  7:367442/136‑1 (MQ=255)
                                                                                                          ttCGGCTCGGTCGACCTGGTCGCGTTGGTGTTCTGGGGTGGGGGGATACCGCCCCTTCCATTCCTGGCCCTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCCTGCCGTGGCCAATATAAGTTGGAGTTCgg           >  3:250471/1‑136 (MQ=255)
                                                                                                          ttCGGCGCGGGCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCgg           <  7:282895/136‑1 (MQ=255)
                                                                                                            cGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGt          <  3:305101/135‑1 (MQ=255)
                                                                                                               cTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTg      <  7:310597/136‑1 (MQ=255)
                                                                                                               cTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTg      <  8:94727/136‑1 (MQ=255)
                                                                                                                tCGGTCGACCTGGTCGCGGTGGTTATCTGGGGTGTGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCTCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTg      <  6:388474/135‑1 (MQ=255)
                                                                                                                  ggTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTgg     <  3:353782/134‑1 (MQ=255)
                                                                                                                   gTCGAGCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGccc  <  8:147905/136‑1 (MQ=255)
                                                                                                                   gTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGccc  <  3:304938/136‑1 (MQ=255)
                                                                                                                   gTCGACCTGGTCGCGTTGGTGAGCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGccc  <  7:218884/136‑1 (MQ=255)
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GGCACTGATCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGCCC  >  ACB122/3267680‑3267930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑