Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,312,455 A→C 28.6% S250A (TCG→GCG)  cynR_3 ← HTH‑type transcriptional regulator CynR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,312,4550AC28.6% 64.6 / 3.8 59S250A (TCG→GCG) cynR_3HTH‑type transcriptional regulator CynR
Reads supporting (aligned to +/- strand):  ref base A (15/25);  new base C (16/3);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 8.86e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCATCATGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCTGCTCCCAGTAGCGCATG  >  ACB122/3312321‑3312588
                                                                                                                                      |                                                                                                                                     
cGCATCATGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGAc                                                                                                                                      <  3:233547/136‑1 (MQ=255)
    tcatGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGGCTCGATGCGCGTGCCCTCCAGCGccccc                                                                                                                                   >  2:115708/1‑133 (MQ=255)
     catGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCACACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATTCGCATGCCCTCCAGCGAccccc                                                                                                                                  >  1:231041/1‑132 (MQ=255)
      atGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTGACCGTCTCGAAGCGCTTGCCCACCCGCGACCCCgg                                                                                                                                 >  1:339929/1‑135 (MQ=255)
      atGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGc                                                                                                                                >  7:87805/1‑136 (MQ=255)
                   cgcACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGt                                                                                                                   >  8:114171/1‑136 (MQ=255)
                       ccGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGTTGGTGCCCGTCTCGCTGCGCGTGCCCGCCCCCGCCCCCGGCCCGGGCCCCCGGGCCg                                                                                                                >  1:176965/1‑135 (MQ=255)
                       ccGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGa                                                                                                               <  3:313275/136‑1 (MQ=255)
                        cGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCACCCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGGCTCGCTGCGCTTGCCCTCCAGCGCCCCCGGCCGGTGCACCCGGTCCgc                                                                                                               >  5:313974/1‑134 (MQ=255)
                           cTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTGCCCGTCTCGATGCGCTTGCCCGCCCGCGCCCCCGGCCGGTGCCCCAGGGCCgcccgg                                                                                                           >  7:17434/1‑131 (MQ=255)
                           cTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCGGATGGTCACCGTCTCGCTGCGCGTGCCCGCCAGCGCCCACGGCCGGGGCACCAGGGCCGACAg                                                                                                            >  5:89355/1‑135 (MQ=255)
                             gCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCCCCGTCTCGCTGCGCGTGCCCTCCAGCGCCCACGGCCGGTGCCCCAGGTCCGCCAGGa                                                                                                          >  5:81325/1‑135 (MQ=255)
                                   ccGGGCTGAAGGCCCGTTCCCGGTCCCAGGCCAACCCCCCGCTCATCGGCGGGACCGGGGCGCTGATGGTCACCGTCGCGAGGCGCTTGCCCGCCAGCCACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCAc                                                                                                   <  4:189021/136‑1 (MQ=255)
                                      ggCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGCCCCCGGCCGGTGCGCCAGGTCCGACAGGATCGCCACTcc                                                                                                >  3:11729/1‑136 (MQ=255)
                                       gCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCCCCCTCGCGCTGCGCGTGCCCTCCAGCGCCCCCGGCCCGTGCCCCCGGGCCGCCAGGATCGCCccgccg                                                                                               >  7:314467/1‑136 (MQ=255)
                                           aaTGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGCCCACGGCCGGTGCCCCAGGTCCGCCAGGATCGCCCCTCCGCTGc                                                                                           >  7:388572/1‑136 (MQ=255)
                                             tgcccgtgcccgGGGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGGCGCTGTTGGACACCGGCGCGATGCGCTGGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGcc                                                                                          <  2:274563/135‑1 (MQ=255)
                                                 ggtgcccGGGCCCAGGCCAACCCCCCGCTCATCGGCGTGAGGGGGGCGCTGATGGTCACCGTCTCGCTGCGCTGGCCCTCCCGCGCCCACGGCCGGTGCACCAGGGCCGACAGGATCGCCACTCCGCTGCCATTgg                                                                                     <  4:227232/132‑1 (MQ=255)
                                                 cGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGGCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCCACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTgg                                                                                     <  4:322837/136‑1 (MQ=255)
                                                   ttCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGCCCACGGCCGGGGGACCAGGTCCGACAGGCTCGCCCCTCCGCTGCCCTTGGc                                                                                    >  5:357810/1‑135 (MQ=255)
                                                      ccGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCGCGATGCGCTTGCCCTCCCGCGCCCCCGGCCGGTGCCCCAGGGCCGCCAGGCTCGCCCCTCCCCGGCCCTTggggcc                                                                                 >  2:381033/1‑131 (MQ=255)
                                                        ggTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGGCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATAGCCACTCCGCTGCCATTGGCGCCCa                                                                               >  5:174911/1‑135 (MQ=255)
                                                                 ccACCCCCCCGCCCACCGCGGTGACGGGGGCGGTGAGGGGCCCGGTCTCTAGGCGCTGCCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCAc                                                                     <  3:17720/136‑1 (MQ=255)
                                                                 ccACCCCCACGCCCAGCGGCGTGACCGGGTCGCTGATGGCCCCCGGCTCGAGGCGCTGCCCCCCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCAc                                                                     <  3:139864/136‑1 (MQ=255)
                                                                 ccAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCAc                                                                     <  8:87805/136‑1 (MQ=255)
                                                                       ccACGCTCATCGGCGTGACCGGGTCGCTGATTGTCACCCTCGCGATTCGCTTGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGCTCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTc                                                               >  4:6261/1‑136 (MQ=255)
                                                                       ccACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGCTCGCCACGCCGCTGCCATTGGCGACCATTCTGCGCACGGccgc                                                               >  7:60068/1‑134 (MQ=255)
                                                                       ccACGCTCATCGGCGTGACCGGGTCGCTGAAGGTCACCGTCTCGATGCCCTTGCCCACCAGCGCCCACCGCCGGTGCCCCAGGGCCGACCTGATCGCCACTCCGCTGCCAATGGCGACCAATCTGCGCCCGGCCTc                                                               >  3:91705/1‑136 (MQ=255)
                                                                         aCGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGCCCCCGGCCGGGGCCCCCGGTCCGACAGGATAGCCACACCGCTGCCCATGGCGCCCATGCTGCGCCCGgccgcc                                                              >  6:196016/1‑135 (MQ=255)
                                                                             tCATCGGCGTGACCGGGTCGCTGATGGTGACCGTCTCGCTTGGCTTGGCCGCCCGCGCCCCCCGCCGGTGGGCCAGGTCCGACAGGATCGCCCCTCCGCGGCCCTTGGCGCCCAAGCTGCGCCCGGCCGCccccc                                                          >  6:349912/1‑134 (MQ=255)
                                                                             tCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGCCCCCGGCCGGTGCCCCAGGTCCGACAGGCTCGCCCCTCCGCTGCCATTGGCGCCCCAGCTGCGCACGGCCGCCACCgc                                                         >  3:340889/1‑135 (MQ=255)
                                                                             tCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACCGCCCGTGCACCAGGGCCGACAGGATCCCCACACCGCTGCCATTTGCGACCAAGCTGCGCCCGGCCTCCACCg                                                          >  5:64849/1‑135 (MQ=255)
                                                                             tCATCGGCGTGACCGGGTCGCTGATGGTCACCCTCTCGATGCGCGTGCCCGCCAGCGCCCACGGCCGGGGCCCCAGGTCCGCCAGGAACCCCCCACCGCTGCCATTGGCGACCCAGCAGCGCACCGCCTCccccc                                                          >  2:385014/1‑134 (MQ=255)
                                                                             tCATCGGCGTGACCGGGTCGCGGATGGTCACCGTCGCGATGCGCTTGCCCGCCCGCGACCACGGCCGGTGCACCAGGTCCGCCAGGCTCGCCACTCCGCGGCCATTGGCGCCCCTGCTGGGCACGGCCACcccc                                                           >  4:324265/1‑134 (MQ=255)
                                                                               aTCGGCGGGACCGGGGCGCTGATGGCCCCCGTCTCGATGCGCTGGCCCCCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGa                                                         <  8:388572/134‑1 (MQ=255)
                                                                                   gCGTGACCGGGTCGCGGATGGGCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                                                   <  8:256522/136‑1 (MQ=255)
                                                                                   gCGTGACCGGGGCGCTGATGGCCCCCGGCTCGCTGCGCTGGCCCTCCACCGCCCACGGCCGGTGCACCAGGGCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                                                   <  8:314467/136‑1 (MQ=255)
                                                                                   gCGTGACCGGGGCGCTGAGGGGCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                                                   <  8:26823/136‑1 (MQ=255)
                                                                                   gCGTGACCGGGGCGCGGATGGGCACCGTCTCGAGGCGCTTGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                                                   <  7:53844/136‑1 (MQ=255)
                                                                                    cGTGACCGGGGCGCGGATGGGCACCGTCTCGATGCGCTTGCCCACCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGt                                                   <  1:32526/135‑1 (MQ=255)
                                                                                        ccgggggCGCTGTTGGGCCCGGTCGCGATGCGCTTGCCCGCCCGCCACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCa                                              <  3:291443/133‑1 (MQ=255)
                                                                                         ccGGGGCGCTGAGGGGCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGGCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCAc                                             <  8:29129/136‑1 (MQ=255)
                                                                                               cgcgGATGGTCACCGTCACGATGCGCTGGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTAc                                       <  8:111420/136‑1 (MQ=255)
                                                                                                      ggccACCGGCCCGAGGCGCTTGCCCGCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTgg                                 <  1:56181/132‑1 (MQ=255)
                                                                                                             gTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCTCCACTCCGCTGCCATTTGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGc                          >  1:313288/1‑135 (MQ=255)
                                                                                                               gtcgtggcgcTTGCCCCCCAGCCCCCACGGCCGGTGCACCAGGACCGACAGGAGCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGccc                        <  2:339929/129‑1 (MQ=255)
                                                                                                                   aggcgcTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCt                   <  4:340889/134‑1 (MQ=255)
                                                                                                                   aTGCGCTTGCCCTCCCGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCt                   <  3:362655/136‑1 (MQ=255)
                                                                                                                    tGCGCTTGCCCCCCCCCCACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCt                   <  5:333440/135‑1 (MQ=255)
                                                                                                                        cTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCTGCTcc              <  7:114171/136‑1 (MQ=255)
                                                                                                                           gCCCTCCAGCGCCCACGGCCGGTGCCCCAGGTCCGCCAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCTGCTCCCa            >  1:190874/1‑135 (MQ=255)
                                                                                                                           gCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCTGCTCCCa            >  1:156180/1‑135 (MQ=255)
                                                                                                                             ccTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCCCTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTTCGCTTGGCCGTTGCCCGGCCTGCTCCGAg           >  8:372675/1‑134 (MQ=255)
                                                                                                                                 cAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCTGCTCCCAGTAgc       <  7:185896/134‑1 (MQ=255)
                                                                                                                                    cGACCACGGCCGGTGCACCAGGTCCGCCAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGTCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCTGCTCCCAGTAGCGCAt   >  6:119495/1‑135 (MQ=255)
                                                                                                                                    cGACCACGGCCGGTGCACCAGGTCCGACAGGATCg                                                                                                       <  7:394836/35‑1 (MQ=255)
                                                                                                                                    cGACCACGGCCGGTGCACCAGGTCCGACAGGATCg                                                                                                       >  8:394836/1‑35 (MQ=255)
                                                                                                                                    cGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGccc                        >  6:126913/1‑114 (MQ=255)
                                                                                                                                    cGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGccc                        <  5:126913/114‑1 (MQ=255)
                                                                                                                                     gACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCTGCTCCCAGTAGCGCATg  <  2:231041/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                     
CGCATCATGGAAGTAATTGCGCACCGCCTGCATCGCCGGGCTGAATGCCCGTTCCCGGTGCCAGGCCAACCCCACGCTCATCGGCGTGACCGGGTCGCTGATGGTCACCGTCTCGATGCGCTTGCCCTCCAGCGACCACGGCCGGTGCACCAGGTCCGACAGGATCGCCACTCCGCTGCCATTGGCGACCATGCTGCGCACGGCCTCCACCGAGCTGGTGCGCACCCTTACGCTTGGCCGTTGCCCGGCCTGCTCCCAGTAGCGCATG  >  ACB122/3312321‑3312588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑