Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	3,413,147	T→G	24.4%	D2A (GAC→GCC)	lexA_2 ←	LexA repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	3,413,147	0	T	G	24.4%	59.7 / 2.3	68	D2A (GAC→GCC)	lexA_2	LexA repressor
Reads supporting (aligned to +/- strand):  ref base T (16/30);  new base G (2/13);  total (18/50)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTCGCGGCGTACGTTCAGCCAGGCGCACAGCGCGGCCATGCTGTCAGCTTCCGCAATTGCCTCGCCATTCAGCCATTTACTGATGGCTTGAGTCGTTTTATGAACCCCGACCATCTTCAGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGTTGATGGTTGCACAGAGCTTGCGCAATAGTCAGTT  >  ACB122/3413013‑3413279                                                                                                                                       |                                                                                                                                     aTTCGCGGCGTACGTTCAGCCAGGCGCACAGCGCGGCCATGCTGTCAGCTTCCGCAATTGCCTCGCCATTCAGCCATTTACTGATGGCTTGAGTCGTTTTATGAACCCCGACCCTCTTCAGGCGTTTGTGGACGt                                                                                                                                      >  5:146559/1‑135 (MQ=255)      cggcgTACGTTCACCCAGGCGCACCGCGCGGCCATGCTGTCAGCTTCCGCAATTGCCGCGCCATTCAGCCATTTACTGATGGCTTGAGTCGTTTTATGAACCCCGACCATCTTCCGGCGTTTGGGGACGTCCACg                                                                                                                                 <  5:375336/135‑1 (MQ=255)       ggcgTACGTTCAGCCAGGCGCACCGCGCGGCCTTGCTGTCAGCTTCCGCAATTCCCTCGCCATTCAGCCATTTACTGATGTCTTGAGTCGTTTTATGACCCCCGACCATCTTCAGGCGTTTGTGGCCGTCCACGcc                                                                                                                               <  4:145254/136‑1 (MQ=255)       ggcgTACGTTCAGCCAGGCGCACAGCGCGGCCATGCTGTCAGCTTCCGCAATTGCCTCGCCATTCAGCCATTTACTGATGGCTTGAGTCGTTTTATTAACCCCGACCATCTTCAGGCGTTTGTGGACGTCCAccc                                                                                                                                >  3:295483/1‑133 (MQ=255)       ggcgTACGTTCAGCCAGGCGCACAGCGCGGCCATGCTGTCAGCTTCCGCAATTGCCTCGCCATTCAGCCATTTACTGATGGCTTGAGTCGTGTTATGAAACCCGACCATGTTCAGGCTGTTGTGGACGTCCaacc                                                                                                                                >  6:224833/1‑132 (MQ=255)                    ccAGGCGCCCCGCGCGGCCATGCGGTCAGCTTCCGCAATTGCCGCGCCATACACCCATTTACTGATGGCTTGAGTCGTTTTATGAACCCCCACCATCTTCGGGGGTTTGGGGCCGCCCACGCCTCTGCCTCGGCt                                                                                                                   <  2:364102/135‑1 (MQ=255)                      aGGCGCACAGCGCGGCCATGCTGTCAGCTTCCGCAATTGCCTCGCCATTCAGCCATTTACTGATGGCTTGAGTCGTTTTATGAACCCCGACCATCTTCAGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGCtt                                                                                                                  >  2:272156/1‑134 (MQ=255)                      aGGCGCACAGCGCGGCCATGCTGTCAGCTTCCGCAATTGCCTCGCCATTCAGCCATTTACTGATGGCTTGAGTCGTGTTATGAACCCCGACCATCTTCAGGCGGTTGTGGACGTCCACGCCTCTGCCTCGGCGTc                                                                                                                 >  4:88318/1‑135 (MQ=255)                           cacaGCGCGGCCATGCTGTCAGCTTCCGCAATTGCCTCGCCATTCAGCCAGTTACTGATGGCTTGAGTCGTTTTATGAACCCCGCCCAGCTTGAGGCGTGTGGGGACGGCCACGCCTCGGCCGCGGCTTCTaaaa                                                                                                            >  6:83840/1‑133 (MQ=255)                              aGCGCGGCCATGCTGTCAGCTTCCGCAATTGCCTCGCCATTCAGCCATTTACTGATGGTTTGAGTCTTTTAATGAACCCCGCCCATCTTCAGGCGTTGGTGGACGGCCACGCCTCTGCCTCGGCTTCTAACACCg                                                                                                         <  6:334419/135‑1 (MQ=255)                                    gCCATGCTGTCAGCTTCCGCAATTGCCTCGCCATTCAGCCATTTACTGATGGCTTGAGTCGTTTTTTGCACCCCGCCCATGTTCCGGCGGTTGTGGACGTCCACGCCGCAGCCTCGGCGTCTAACCCCGGCAtcgt                                                                                                  >  8:394603/1‑136 (MQ=255)                                    gCCATGCTGTCAGCTGCCGCAATTGCCTCGCCATACAGCCATTTCCTGAGGGCTTGAGTCGTTTTATCAACCCCGACCATCTTCCGGCGTTTGTGGACGGCCACGCCTCTGCCTCGGCTTCTAACACCGGCAtcgt                                                                                                  <  3:16711/136‑1 (MQ=255)                                     ccATGCTGTCAGCTTCCGCAATTGCCTCGCCATTCAGCCATTTACTGATGGCTTGAGTCGTTTTATGAACCCCGACCATCTTCAGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCAtcgt                                                                                                  <  6:278103/135‑1 (MQ=255)                                        tGCTGTCAGCTTCCGCAATTGCCTCGCCATTCAGCCATTTACTGATGGCTTGAGTCGTTTTATGAACCCCGCCCATCTTCAGGCGTTTGTGGACGACCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGa                                                                                               <  1:272156/135‑1 (MQ=255)                                           tGTCAGCTTCCGAAATTGCCTCGCCATTCAGCCATTTACTTATGGCTTGAGTGGTTTTATGAACCCCGACCATCTTCAGGCGTTTGGGGACGGCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAgg                                                                                             <  5:254970/134‑1 (MQ=255)                                                  ttCCGCAATTGCCTCGCCATTCAGCCATTTACTGATGGCTTGAGTCGTTTTTTGAACCCCGCCCCTCTTCAGGCGGTTGTGGTCGTCCCCGCCTCTGCCGCGGGGTCGAAAACCGGCATCGGCGAGGGCCGCATg                                                                                     >  2:260985/1‑135 (MQ=255)                                                  ttCCGCAATTGCCTCGCCATTCAGCCATTTACTGATGGCTTGAGTCGTTTTATGAACCCCGACCATCTTAACGCGTTTGTGGTCGTCCACTCCTCTGCCTCGGCATCTAACACCGGGATCGTAGAGGGCCTCATg                                                                                     >  4:299545/1‑135 (MQ=255)                                                    cggtaaTTGCCTAGCCATTTTGCATTTTACTGTTGTTTTGATGGGTTTTATTTCGGCCGAACATCTTCAGGGTTTTGTGGAATTCCACGGCTCTGCCTCGGCTTCTTACAAGGGCATCGTCGAGGGCCTAATGTa                                                                                   <  8:126371/131‑1 (MQ=255)                                                     cGCAATTGCCTCGCCATTCAGCCATTTACTGATGGCTTGAGTCGTTTTATGAACCCCGACCATCTTCAGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTa                                                                                   <  6:318243/134‑1 (MQ=255)                                                     cGCAATGGCCTCGCCATTCAGCCATTTACTGATGGCTTGAGTCGTTTTATGAACCCCGACCATCTTCAGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTa                                                                                   <  6:360604/134‑1 (MQ=255)                                                      gaaaTTGCGTCGCCATTCAGCAATTTAGTGATGGATTTAGGCGTTTTATGAACCCCGACCATCTTCAGGCTTTTGTGGACGGCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGt                                                                                 <  1:45514/133‑1 (MQ=255)                                                      gCAATTGCCTCGCCATTCAGCCATTTACTGATGGCTTGAGTCGTTTTATGAACCCCGACCATCTTCAGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGt                                                                                 <  6:71093/135‑1 (MQ=255)                                                        aaTTGCCTCGCCATTCAGCCATTTACTGATGGCTTGAGTCGTTTTATGAACCCCGCCCATCTTCAGGCGTTTGTGGTCGTCCACGCCTCTGCCTCGGGTTGGAAAACCCGCATCGGGGGGGGGCGCATGGAGTCg                                                                               >  2:308203/1‑135 (MQ=255)                                                             ccTCGCCATTCAGCCATTTACTGATGTCTTGAGTCGTTTTATGAACCCCGACCAGCTTCAGGCGTTTTTGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTgcgc                                                                          <  7:58690/135‑1 (MQ=255)                                                                        aGCCATTTACTGATGGCTTGAGTCGTTTTATGAACCCCGACCAGCTTCAGGCGTTTGTGGACGGCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTgc                                                                <  2:25187/134‑1 (MQ=255)                                                                         gCCATTTACTGATGGCTTGAGTCGTTTTATGACCCCCGACCATCTTCCGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGcgcg                                                             <  3:88318/136‑1 (MQ=255)                                                                           cATTTACTGATGGCTTGAGTCGTTTTATGAACCCCGACCATCTTCAGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGt                                                            <  6:325549/135‑1 (MQ=255)                                                                                aCTGATGGCTTGAGTCGTGTTATGCACCCCGCCCATCTTCAGGCGTTTGTGGTCGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGCGGGCCTCATGGAGTCGTGCGCGGCAGGCTGCGCGGAggtg                                                       >  5:68939/1‑132 (MQ=255)                                                                                    aTGGCTTGAGGCGTTTTATGAACCCCGACCATCTTCAGGCGTTTGGGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCAtttt                                                  <  4:352553/136‑1 (MQ=255)                                                                                       gCTTGAGTCGTTTTTTGAACCCCGACCATCTTAAGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGGTTATAACACCGGGATAGTCGAGGGCCTGATGTAGTCGTGAGCTGAAGGCAGCGCGTAGCTCAtttttt                                                >  8:350675/1‑134 (MQ=255)                                                                                       gCTTGAGTCGTTTTATGAACCCCGACCATCTTCAGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCGCATGTAGTCGTGCGCGGAAGGCGTCGCGTAGCTCATTTTTa                                                >  3:153516/1‑135 (MQ=255)                                                                                       gCTTGAGTCGTTTTATGAACCCCGACCATCTTCAGGCGTTTGTGGACGGCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCAGCGCGGAGCGCCATTTTa                                                >  7:326900/1‑135 (MQ=255)                                                                                       gCTTGAGTCGTGTTATGAACCCCGACCATCTTCCGGCGGTTGTGGCCGTCCCCCCCTCGGGCTCGCGGTCTAACACCGGCCTCGTCGAGGGGCTGAGGGATGGGTTGGGTGAAGGCGGCGCCGAGCGCAtttttt                                                >  2:333527/1‑134 (MQ=255)                                                                                         ttGAGTCGTTTTATGAACCCCGACCATCTTCAGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTAta                                              >  8:138453/1‑134 (MQ=255)                                                                                          tGAGTCGTTTTATCACCCCCGACCATCTTCAGGCGTGTGGGGCCGCCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCa                                             <  1:186281/135‑1 (MQ=255)                                                                                           gAGTCGTTTTATGAACCCCGACCATCTTCAGGCGTGTGGGGACGGCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCaa                                            <  7:227904/135‑1 (MQ=255)                                                                                            aGTCTTTTTATGAACCCCCACCATCTGCCGGCGTTTGTGCCCGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCaa                                            <  5:243572/134‑1 (MQ=255)                                                                                            aGTCTTATTATCAACCCCCACCATCTTCAGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAAc                                           <  7:350675/135‑1 (MQ=255)                                                                                               cGTGTTATGAACCCCGCCCATCTTCAGGCGGTTGTGGACGTCCACGCCTCTGCCTCGGCGTCTAACACCGGCATCGGCGCGGGCCTCATGTAGTCGTGCGCGGAAGGCTGCGCGTAGGTCATTTTTTTAAACCaag                                       >  3:104413/1‑134 (MQ=255)                                                                                                tttttATCAACCCCGACAATCTTCAGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATgg                                      <  7:159742/135‑1 (MQ=255)                                                                                                gTTTTATCACCCCCGACCATCTACTGGCGTTTGGGGACGCCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATgg                                      <  7:254207/136‑1 (MQ=255)                                                                                                 ttttATGAACCCCGACCATCTTCAGGCGTTTGTGCACGGCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATgg                                      <  5:67888/135‑1 (MQ=255)                                                                                                  tttATGAACCCCGCCCAGCTTCAGGCGTTTGGGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATgg                                      <  8:147481/134‑1 (MQ=255)                                                                                                  tttATGAACCCCGACCATCTTCAGGCTTTTGTGGAGGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGt                                    <  8:232274/136‑1 (MQ=255)                                                                                                   ttATGCCCCCCGACCAGGTTCCGGCTTTTGGGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCTTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGt                                    <  7:233354/135‑1 (MQ=255)                                                                                                   ttATGAACCCCGACCATCTTCAGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGt                                    <  8:317249/135‑1 (MQ=255)                                                                                                    tATGAACCCCGACCATCTTCCGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGt                                    <  2:207569/134‑1 (MQ=255)                                                                                                    attGAACCCCGACGATCTTCTGGCGTTTGTGTCCGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGt                                    <  5:160678/132‑1 (MQ=255)                                                                                                       gAACCCCGACCATCTTCAGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGTTGa                                 <  6:127046/134‑1 (MQ=255)                                                                                                       gAACCCCGACCATCTTCAGGCGTTTGTGGACGGCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGTTGa                                 <  1:242595/134‑1 (MQ=255)                                                                                                       gAACCCCGACCATCTTCAGGCGGTTGTGGACGGCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGTTGa                                 <  6:311478/134‑1 (MQ=255)                                                                                                          ccccGACCATCTTCCGGCGTTTGGGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGTTGATGGtt                            <  4:256873/136‑1 (MQ=255)                                                                                                          ccccGACCATCTTCAGGCGTTTGTGGACGGCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGTTGATGGtt                            <  3:131273/136‑1 (MQ=255)                                                                                                           cccGACCATCTTCAGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGTTGATGGtt                            <  1:10556/135‑1 (MQ=255)                                                                                                           cccGACCATCTTCAGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGTTGATGGtt                            <  6:138620/135‑1 (MQ=255)                                                                                                           cccGACCATCTTCAGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGTTGATGGTTg                           <  4:272587/136‑1 (MQ=255)                                                                                                            ccGACCTGCTTCCGGCGTTGGGGCCCGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGGCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTCTCAACCATGGGTTGATGGTTg                           <  5:83840/135‑1 (MQ=255)                                                                                                              gACCATCTTCAGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGTTGATGGTTGca                         >  7:89270/1‑135 (MQ=255)                                                                                                              gACCATCTTCAGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGTTGATGGTTGca                         <  1:308203/135‑1 (MQ=255)                                                                                                              gACCATCTGCAGGCGTTTGGGGCCGCCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGTTGATGGTTGcac                        <  4:266651/136‑1 (MQ=255)                                                                                                                 cATCTTCAGGCGTTTGTGGACGGCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGTTGATGGTTGCACag                      <  1:208126/135‑1 (MQ=255)                                                                                                                 cATCTGCCGGCGTTTGGGGACGCCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATTGGTTGATGGTTGCACag                      <  5:127051/135‑1 (MQ=255)                                                                                                                   tCTTCAGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGTTGATGGTTGCACagag                    >  2:158305/1‑135 (MQ=255)                                                                                                                       cAGGCGTTTGTGGAGGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGTTGATGGTTGCACAGAGCtt                 <  8:58619/134‑1 (MQ=255)                                                                                                                       cAGGCGTTTGTGGACGGCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGTTGATGGTTGCACAGAGCtt                 <  4:295483/134‑1 (MQ=255)                                                                                                                        cggCGTTTGTGCACGCCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGTTGATGGTTGCACAGAGCtt                 <  1:161451/132‑1 (MQ=255)                                                                                                                                   gACGGCCACGCCTCTGCCTCGGCTTCTAACTCCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGTTGATGGTTGCACAGAGCTTGCGCAATAGTCAg    <  1:163767/135‑1 (MQ=255)                                                                                                                                    aCGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGACTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGTTGATGGTTGCACAGAGCTTGCGCAATAGTCAg    <  5:224833/134‑1 (MQ=255)                                                                                                                                     cGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGTTGATGGTTGCACAGAGCTTGCGCAATAGTCAGtt  <  6:144233/135‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     ATTCGCGGCGTACGTTCAGCCAGGCGCACAGCGCGGCCATGCTGTCAGCTTCCGCAATTGCCTCGCCATTCAGCCATTTACTGATGGCTTGAGTCGTTTTATGAACCCCGACCATCTTCAGGCGTTTGTGGACGTCCACGCCTCTGCCTCGGCTTCTAACACCGGCATCGTCGAGGGCCTCATGTAGTCGTGCGCTGAAGGCTGCGCGTAGCTCATTTTTATCAACCATGGGTTGATGGTTGCACAGAGCTTGCGCAATAGTCAGTT  >  ACB122/3413013‑3413279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑