Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,532,861 A→G 7.4% K164R (AAG→AGG)  CJ019_03352 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,532,8610AG 7.4% 165.7 / 4.3 55K164R (AAG→AGG) CJ019_03352hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (28/22);  new base G (2/2);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-01

ACCCTGACCACCTACGTGCGCGGCCGTGGTGAAGCGACCGTGGCGTTCAATGTCTCGTTCGAGCACAAACAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAGGCCCACGCCAATGCCTTGCCCGAGC  >  ACB122/3532728‑3532995
                                                                                                                                     |                                                                                                                                       
aCCCTGACCACCTACGTGCGCGGCCGTGGTGAAGCGACCGTGGCGTTCAATGTCTCGTTCGAGCACAAACAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAag                                                                                                                                        <  6:90662/135‑1 (MQ=255)
  ccTGACCACCTACGTGCGCGGCCGTGGTGAAGCGACCGTGGCGTTCAATGTCTCGTTCGAGCACAAACAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAagca                                                                                                                                      >  6:140721/1‑135 (MQ=255)
  ccTGACCACCTACGTGCGCGGCCGTGGTGAAGCGACCGTGGCGTTCAATGTCTCGTTCGAGCACAAAAAGCCCCAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGAAGCTGTTCCTCAACGAAAACCTCAagcag                                                                                                                                     >  3:76792/1‑136 (MQ=255)
   cTGACCACCTACGGGGGCGGCCGGGGTGAAGCGACCGTGGCGTTCAATGTCTCGTTCGAGCACAAACAGCCACAAGTAGCCAAGGAAGGCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAagcagc                                                                                                                                    <  8:373311/136‑1 (MQ=255)
              cGTGCGCGGCCGTGGTGAAGCGACCGTGGCGTTCAATGTCTCGTTCGAGCACAAACAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACcgag                                                                                                                            >  5:275985/1‑133 (MQ=255)
                     ggCCGTGGTGAAGCGACCGTGGCGTTCAATGTCTCGTTCGAGCACAAACAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCaa                                                                                                                    >  1:373715/1‑134 (MQ=255)
                       ccGTGGTGAAGCGACCGTGGCGTTCAATGTCTCGTTCGAGCACAAACAGCCCCAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACg                                                                                                                  >  2:123434/1‑134 (MQ=255)
                       ccGTGGTGAAGCGACCGTGGCGTTCAATGTCTCGTTCGAGCACAAACAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACg                                                                                                                  >  2:325618/1‑134 (MQ=255)
                        cGTGGTGAAGCGACCGTGGCGTTCAATGTCTCGTTCGAGCACAAACAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGaaa                                                                                                               >  3:87418/1‑136 (MQ=255)
                                       gTGGCGTTCAATGTCTCGTTCGAGCACAAACAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCa                                                                                                >  3:318362/1‑136 (MQ=255)
                                         ggCGTTCAATGTCTCGTTCGAGCACAAACAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAGGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCAcc                                                                                              >  7:115277/1‑136 (MQ=255)
                                         ggCGTTCAATGTCTCGTTCGAGCACAAACAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAGGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCAcc                                                                                              >  7:120611/1‑136 (MQ=255)
                                         ggCGTTCAATGTCTCGTTCGAGCACAAACAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCaa                                                                                                                    >  4:142858/1‑114 (MQ=255)
                                         ggCGTTCAATGTCTCGTTCGAGCACAAACAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCaa                                                                                                                    <  3:142858/114‑1 (MQ=255)
                                            gTTCAATGTCTCGTTCGAGCACAAACAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCa                                                                                            <  1:257436/135‑1 (MQ=255)
                                            gTTCAATGTCTCGTTCGAGCACAAACAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCa                                                                                            <  5:218168/135‑1 (MQ=255)
                                                 aTGTCTCGTTCGAGCCCAAACAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAACCCTCAAGCAGCGCACCGACCGAGCCCACGAAACCACCGAAATCCTAACCCAGGAAg                                                                                       >  1:11927/1‑135 (MQ=255)
                                                        gTTCGAGCACAAACAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCcaacaa                                                                                <  4:87418/135‑1 (MQ=255)
                                                         ttCGAGCACAAACAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCcaacaa                                                                                <  2:310417/134‑1 (MQ=255)
                                                          tCGAGCACAAACAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCcaacaa                                                                                <  2:31271/133‑1 (MQ=255)
                                                          tCGAGCACAAACAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCcaacaa                                                                                >  1:31271/1‑133 (MQ=255)
                                                          tCGAGCACAAACAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTACCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGc                                                                              >  1:384648/1‑135 (MQ=255)
                                                                 caAACAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGc                                                                       >  1:328357/1‑135 (MQ=255)
                                                                     cAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAg                                                                   >  1:217335/1‑135 (MQ=255)
                                                                       gCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTg                                                                >  8:65021/1‑136 (MQ=255)
                                                                                   cAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACACGCTGGGTGCCGAGCTGGCCAGCC‑Tgg                                                     >  1:196645/1‑134 (MQ=255)
                                                                                         aGTCGCCGATGAACTTGTTAAGCTGTTCCTCAAAGAAAACCTAAATCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTACTCACCCAGGAAGCCAACAAGCTGGGTGCCGACCAGGCCAGCCATGCAAAAcag                                              >  8:340334/1‑134 (MQ=255)
                                                                                         aGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCa                                              >  1:351111/1‑135 (MQ=255)
                                                                                            cGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTg                                          <  7:340334/136‑1 (MQ=255)
                                                                                             gCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGa                                                                                                                 >  8:352372/1‑65 (MQ=255)
                                                                                             gCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGa                                                                                                                 <  7:352372/65‑1 (MQ=255)
                                                                                                      cTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTc                                 <  1:84165/135‑1 (MQ=255)
                                                                                                       tGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCa                                <  2:373715/135‑1 (MQ=255)
                                                                                                            aCGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGCGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAgg                          >  8:310139/1‑136 (MQ=255)
                                                                                                            aCGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCa                                                                                            <  3:155508/71‑1 (MQ=255)
                                                                                                            aCGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCa                                                                                            >  4:155508/1‑71 (MQ=255)
                                                                                                            aCGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAg                           <  1:325618/135‑1 (MQ=255)
                                                                                                            aCGCTGTTCCTCAACG‑AAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAgg                          >  2:69843/1‑135 (MQ=255)
                                                                                                             cGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAg                           <  5:140721/134‑1 (MQ=255)
                                                                                                             cGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAg                           <  6:275985/134‑1 (MQ=255)
                                                                                                                 gTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAGGCCCAc                     >  4:199071/1‑136 (MQ=255)
                                                                                                                 gTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAGGCCCAc                     >  7:5834/1‑136 (MQ=255)
                                                                                                                  ttCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGGGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAGGCCCACg                    <  3:120656/136‑1 (MQ=255)
                                                                                                                     cTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAGGCCCACGc                   >  1:300120/1‑134 (MQ=255)
                                                                                                                      tCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCa                                                                                            >  6:292089/1‑61 (MQ=255)
                                                                                                                      tCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCa                                                                                            <  5:292089/61‑1 (MQ=255)
                                                                                                                      tCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAGGCCCACGCCa                 >  5:40461/1‑135 (MQ=255)
                                                                                                                           gAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAGGCCCACGCCAATGcc            >  2:349493/1‑135 (MQ=255)
                                                                                                                             aaaCCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAGGc                         >  3:210198/1‑120 (MQ=255)
                                                                                                                             aaaCCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAGGc                         <  4:210198/120‑1 (MQ=255)
                                                                                                                             aaaCCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAGGCCCACGCCAATGCCTTg         <  8:5834/136‑1 (MQ=255)
                                                                                                                             aaaCCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAGGCCCACGCCAATGCCTTg         >  4:376773/1‑136 (MQ=255)
                                                                                                                              aaCCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAGGCCCACGCCAATGCCTTg         <  1:69843/135‑1 (MQ=255)
                                                                                                                              aaCCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAGGCCCACGCCAATGCCTTg         <  2:351111/135‑1 (MQ=255)
                                                                                                                               aCCTCAGGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAGGCCCACGCCAATGCCTTGcc       <  8:115277/136‑1 (MQ=255)
                                                                                                                               aCCTCAGGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAGGCCCACGCCAATGCCTTGcc       <  8:120611/136‑1 (MQ=255)
                                                                                                                               aCCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAGGCCCACGCCAATGCCTTGcc       <  3:44615/136‑1 (MQ=255)
                                                                                                                               aCCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAAGTCCTCACCCCGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAGGCCCACGCCAATGCCTTGcc       <  8:179104/136‑1 (MQ=255)
                                                                                                                                catCATGCAGCGCACAGAGCGAGCCAACTAAACCACCTAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAGGCCCACGCCAATGCCTTTcc       <  2:392367/133‑1 (MQ=255)
                                                                                                                                    aaGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGACAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAGGCCCACGCCAATGCCTTGCCCGAGc  <  3:213264/136‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
ACCCTGACCACCTACGTGCGCGGCCGTGGTGAAGCGACCGTGGCGTTCAATGTCTCGTTCGAGCACAAACAGCCACAAGTAGCCAAGGAAGTCGCCGATGAACTGGTGACGCTGTTCCTCAACGAAAACCTCAAGCAGCGCACCGAGCGAGCCAACGAAACCACCGAATTCCTCACCCAGGAAGCCAACAAGCTGGGTGCCGAGCTGGCCAGCC‑TGGAAAACCAGCTGGCCGATTTCAAGCAGGCCCACGCCAATGCCTTGCCCGAGC  >  ACB122/3532728‑3532995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑