Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	336,468	T→G	26.2%	K9N (AAA→AAC)	CJ019_00309 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	336,468	0	T	G	26.2%	96.9 / 9.4	63	K9N (AAA→AAC)	CJ019_00309	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (12/33);  new base G (16/2);  total (28/35)
Fisher's exact test for biased strand distribution p-value = 9.04e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAGCTGCCCGACTCCATGGCCTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTGTTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACA  >  ACB122/336336‑336601                                                                                                                                     |                                                                                                                                      gtAGCTGCCCGACTCCATGGCCTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGttt                                                                                                                                     <  3:41113/135‑1 (MQ=255) gtAGCTGCCCGACTCCATGGCCTGGTTGTACACCTGCTCGACGCTGGCGCTGGCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGttt                                                                                                                                     <  5:15463/135‑1 (MQ=255)    gCTGCCCGACTCCATGGCCTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTg                                                                                                                                  <  4:365592/135‑1 (MQ=255)           gACTCCATGGCCTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTGTTGCTTgg                                                                                                                          >  8:126597/1‑136 (MQ=255)             cTCCATGGCCTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTg                                                                                                                                  >  4:306850/1‑126 (MQ=255)             cTCCATGGCCTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTg                                                                                                                                  <  3:306850/126‑1 (MQ=255)              tCCATGGCCTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTGTTGCTTGGTa                                                                                                                        <  1:229504/135‑1 (MQ=255)                cATGGCCTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTGTTGCTTGGTAgg                                                                                                                      <  8:218658/135‑1 (MQ=255)                cATGGCCTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTGTTGCTTGGTAg                                                                                                                       <  3:223587/134‑1 (MQ=255)                 aTGGCCTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGGACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTGTTGCTTGGTAg                                                                                                                       <  6:159236/133‑1 (MQ=255)                   ggCCTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCGTGGCGCGTTTCTGTTTGGTGGTAGGGGTg                                                                                                                  >  3:88753/1‑136 (MQ=255)                   ggCCTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCGTGGCGCGGTTGTGTTGGTTGGTAgggggg                                                                                                                  >  7:78473/1‑134 (MQ=255)                    gCCTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTGTTGCTTGGTAGGGGTgg                                                                                                                 <  3:115866/136‑1 (MQ=255)                     ccTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTGTTGCTTGGTAGGGGTgg                                                                                                                 <  5:44999/135‑1 (MQ=255)                          ttgtACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTGTTGCTTGGTAGGGGTGGCCATg                                                                                                            <  8:61533/135‑1 (MQ=255)                           tgtACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGGGCGGTTGTGGTGCTTGGTAGGGGGGGGCGTggg                                                                                                          >  7:364843/1‑136 (MQ=255)                           tgtACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTGTTGCTTGGTTGGGGTGGCCATggg                                                                                                          >  4:159451/1‑136 (MQ=255)                           tgtACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTGTTGCTTGGTAGGGGTGGCCATg                                                                                                            <  5:370529/134‑1 (MQ=255)                             tACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTGTGTTGCTTGGTAGGGGTGGCCATgggg                                                                                                         >  3:179007/1‑135 (MQ=255)                             tACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCGTGGCGCGGTTGGGGTGGTTGGTTGGGGTGGGCATggg                                                                                                          >  1:298382/1‑134 (MQ=255)                                acCTGCTCGACGCTGGCGCTGGCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTGTTGCTTGGTAGGGGTGGCCATGGGGaaa                                                                                                      <  2:39537/135‑1 (MQ=255)                                  cTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTg                                                                                                                                  <  4:239462/105‑1 (MQ=255)                                  cTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGGTTCTg                                                                                                                                  >  3:239462/1‑105 (MQ=255)                                  cTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCGTGGCGCGGTTGTGGTGGGTGGTAGGGGGGGCGCTGGGGAAAtg                                                                                                    >  6:370529/1‑134 (MQ=255)                                     cTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTGTTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTg                                                                                                <  4:267055/136‑1 (MQ=255)                                     cTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTGTGTGTTGGTTGGGAGGGGTGGGCATGGGGAAATCCtt                                                                                                 >  6:168577/1‑135 (MQ=255)                                      tCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTGTTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTg                                                                                                <  5:318164/135‑1 (MQ=255)                                       cGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGGGCGGTTCTGTTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTggg                                                                                              >  3:118593/1‑136 (MQ=255)                                       cGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCGTGGCGCGTTTCTGGTGCGTGGGAGGGGTGGCCCT‑GGGAAATCCTTGGGt                                                                                             >  8:223814/1‑136 (MQ=255)                                          cGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGttt                                                                                                                                     <  7:85503/94‑1 (MQ=255)                                          cGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGttt                                                                                                                                     >  8:85503/1‑94 (MQ=255)                                                cgcTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTGTTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTc                                                                                        <  1:210607/133‑1 (MQ=255)                                                  cTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCGTGGCGCGGGTGTGGTGGGTTGGAGGGGGGGGCCTGGGGGAATCCGTGGGGGCGTCTgga                                                                                    >  5:82400/1‑133 (MQ=255)                                                              cTGGCCGTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTGTTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTg                                                                       <  8:303431/136‑1 (MQ=255)                                                                  ccTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGGGGTTAGGTTGGAGTGTATGGGGGGCCCTGTGGCAATACATGGGTTCGTCGTAAAGTTAGCGTATTGAAg                                                                    >  2:236129/1‑135 (MQ=255)                                                                  ccTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTGTGTTGCTTGGTTGGGGTGGGCAGGGGGAAATCCTTGGGTGTGTCTGAAAGTGCGCGTATTGCAgc                                                                   >  4:143327/1‑136 (MQ=255)                                                                    ttGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCGTGGCGCGTTTGGGTTGCTTGGTAGGGGGGGGCCTGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAgcgc                                                                 >  8:18407/1‑136 (MQ=255)                                                                    ttGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCGTGGCGCGTGTGTGTTGGTTGGGAGGGGTGGCCATGGGGAAATGCGTGGGTGAGTGTGAAAGTGCGCGGATTGCAgccc                                                                 >  4:312031/1‑134 (MQ=255)                                                                      gACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCGTGGCCTTGCTGGCAGCGCGCTTGGCGCGTTTCTGTTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCaa                                                               <  8:84205/136‑1 (MQ=255)                                                                         cGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTGTGTTGGTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTg                                                            >  8:14269/1‑136 (MQ=255)                                                                              ggCCGCTGCGTACCCTGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTGTTGGTTGGTAGGGGTGGCCATGGGGAAATCGTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCg                                                        <  2:329541/135‑1 (MQ=255)                                                                              ggCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGGGGGGTTGTGGTGCGTGGTAGGGGGGGGGATGGGGAAATGCTGGGGTGAGGCGGTAAGTTGGCGTTTTGAAGCGCAATTGCCCGa                                                       >  7:15793/1‑136 (MQ=255)                                                                              ggCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCGTGGGGCGGGTCGGGTGGGTGGTTGGGGGGGGCCAGGGGGCATGCGTGGGTGTGTGGGTAAATGCGGCGATTGAAGCGCAATTGccc                                                         >  2:82095/1‑134 (MQ=255)                                                                                      cGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGGTTCGGGTGGTTGGTTGGGGTGGCCATGGGGCAATGCTTGGGTGAGGCTGAAAGTGGGGGTATTGAAGCGCAATTGCCCGATAAAccc                                                >  8:59955/1‑135 (MQ=255)                                                                                      cGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCGTGGCGCGGTTGTGTTGCGTGGGTGGGGTGGCCATGGGGAAATGCTTGGGTGCGTCTGAAAGTGCGCGTATTGAAGCGCAAGTGCCCGCTAAAccc                                                >  3:138155/1‑135 (MQ=255)                                                                                        tACCATGCGGTTCTGCTGGGCCTTGCTGGCGGCGCGCTGGGCGCGTTTCTGTTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAg                                             <  3:144859/136‑1 (MQ=255)                                                                                           cATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCGTGGCGCGGTTCTGTTGCGTGGTTGGGGTGGGCATGGGGGAATGCGTGGGTGAGTTTGAAAGTGCGCGTATTGTAGCGCAATTTCCCGATAAACCCAAgcg                                           >  5:70929/1‑135 (MQ=255)                                                                                                       cgtGGCCTTGCTGGCGGCGCGCTTGGCGCGTGTCTGTTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGgcg                              <  4:269286/134‑1 (MQ=255)                                                                                                       cTTGGCCTTGGGGGCGGGGCGGTTGGCGGGTTTCTGTTGCTGGGCCGGGGGGGCCTTGGGTAAACCCTTGGGTGAGTCTGAAAGTGCGCGTATTCACGCGAAATGGCCCGAAAACCCCAAGCGGCGCGCCTGGgc                               <  6:266084/135‑1 (MQ=255)                                                                                                       cTTGGCCTTGCTGGCGGCGCGCTTGGCGCGGTTCTGTTGCTTGGTAGGGGGGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGgc                               <  6:159565/135‑1 (MQ=255)                                                                                                       cTTGGCCTTGCTGGCGGCGCGCTTGGCGCGGTTCTGTGGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGgc                               <  5:314614/135‑1 (MQ=255)                                                                                                        ttGGCCTTGCTGGCGGCGCGCGTGGCCCGTTTGGGTTGCGTTGTATGGGTGGCCATTGGGAAATCCTTTGGTTAGTCTGTAATTGCCCCGATTGAAGCGCAATTGCCCGATAAACCCAACCGGCGCCCCAGGgcg                              >  6:327694/1‑135 (MQ=255)                                                                                                        ttGGCCTTGCGTGCGGCGCTCGTGGCGCGTTTCTGTTGCTTGGTCGGGGTGGCAATGGGTAAATCCTTGGGGGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGgc                               <  6:20326/134‑1 (MQ=255)                                                                                                          ggCCTTGCTGGCGGCGCGCTTGGCGCGGTTCTGTTGCGTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGGATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTg                           >  3:269286/1‑136 (MQ=255)                                                                                                             cTTGCTGGCGGCGCGCTGGGCGCGTTTCTGTTGCTTGGTAGGGGGGGCCATGGGGAAATCCTTGGGGGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCa                         <  2:246772/135‑1 (MQ=255)                                                                                                               tGCTGGCGGCGCGCTTGGCGCGGTTCTGTTGCGTGGTAGGGGTGGCCATGGGGAAATCCTTGGGGGAGTCTGAAAGTGCGGGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCCCCGGGGCGCTGCAGCt                      >  8:187682/1‑136 (MQ=255)                                                                                                                 cTGGCGGCGCGCTTGGCGCGTTTCGGTTGCTTGGTAGGGGGGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCt                      <  1:223698/134‑1 (MQ=255)                                                                                                                    gcggcgCGCTTGGCGCGTTTCTGTTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTgaga                 <  8:78473/136‑1 (MQ=255)                                                                                                                    gcggcgCGCTTGGCGCGGTTCTGTTGCTTGGTTGGGGTGGCCATGGGGAAATGCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCt                      >  1:212114/1‑131 (MQ=255)                                                                                                                     cggcgCGCTTGGCGCGTTTCTGTTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGGGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTga                   <  1:111460/133‑1 (MQ=255)                                                                                                                     cggcgCGCTTGGCGCGTGTCTGTTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGCGTCTGAAAGTGCTCGTATTTAAGCGCAATTGCGCTATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGAt                <  3:343386/136‑1 (MQ=255)                                                                                                                     cggcgCGCTGGGCGCGTTTCGGTGGCGTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGATTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACACAAGCGGCGCGCCTGGGCGCTGCAGCTTgaga                 <  2:103002/135‑1 (MQ=255)                                                                                                                       gcgcgcTTGGCGCGTTTCTGTTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGt              <  7:81655/136‑1 (MQ=255)                                                                                                                           gcTTGGTGCGTTGCTGTTGCTTGGTAGGGGTGCCCAGGGGTAAAGCCTTGGGTGAGTCTGAAAGTGCGCGTATTGACGCGCAATTGCCCGATAACCCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCtg           <  5:327694/135‑1 (MQ=255)                                                                                                                             gtGGCGCGTTTCTGTGGCTTGGTAGGGGGGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCtgtg         <  6:121641/134‑1 (MQ=255)                                                                                                                                gcgcGTTTCTGTTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTggg       <  1:238439/134‑1 (MQ=255)                                                                                                                                    gTTTCGGTGGCGTGGTCGGGGGGGCCATGGGGAAATCCTTGGGGGAGCCTGAAAGTGCGCGTATTGACGCGAAATTGCCCGATAAACCCACGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACa  <  2:363188/135‑1 (MQ=255)                                                                                                                                     |                                                                                                                                      GTAGCTGCCCGACTCCATGGCCTTGTTGTACACCTGCTCGACGCTGGCGCTGTCGCCGGCACTGGCCTTGACCGCTTGGCCGCTGCGTACCATGCGGTTCTGCTTGGCCTTGCTGGCGGCGCGCTTGGCGCGTTTCTGTTGCTTGGTAGGGGTGGCCATGGGGAAATCCTTGGGTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACA  >  ACB122/336336‑336601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑