| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 3,731,291 | T→A | 17.1% | intergenic (+38/‑14) | cnrA → / → fabF_3 | Nickel and cobalt resistance protein CnrA/3‑oxoacyl‑[acyl‑carrier‑protein] synthase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,731,291 | 0 | T | A | 17.1% | 73.6 / 3.0 | 33 | intergenic (+38/‑14) | cnrA/fabF_3 | Nickel and cobalt resistance protein CnrA/3‑oxoacyl‑[acyl‑carrier‑protein] synthase 2 |
| Reads supporting (aligned to +/- strand): ref base T (8/19); new base A (4/2); total (12/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTTGGTGTTCCTGCCGGCGATGTATTCCATCTGGTTCAAGATCCGCCCCAATAGCCGCATTGCCAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCGCGCCCGATGCAATTCGAGGCGCGCTCATGATCCAACGAGAGGTCGAAATGACTCACAACGTTAATCAAAAGCGCATCGTGGTGACCGGCACCGGCGTTGTCAGCCCGCTGGGCTGCGGCGTAAAGGAAGTCTGGCGGCGATTGCTG > ACB122/3731162‑3731424 | ccTTGGTGTTCCTGCCGGCGATGTATTCCATCTGGTTCAAGATCCGCCCCAATAGCCGCATTGCCAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCGCGCCCGATGCAATTCGAg < 5:132210/135‑1 (MQ=255) cTTGGTGTTCCTGCCGGCGATGTATTCCATCTGGTTCAAGATCCGCCCCAATAGCCGCATTGCCAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCCCGCCCCATGCAAATCCAGgc > 7:38244/1‑136 (MQ=255) gtTCCTGCCGGCGATGTATTCCATCTGGTTCAAGATCCGCCCCAATAGCCGCATTGCCAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGCCATTGCCTTCAACCCGTGCCCCCCCGATGCAAATAGCGgcgccccc > 4:207152/1‑132 (MQ=255) gtTCCTGCCGGCGATGTATTCCATCTGGTTCAAGATCCGCCCCAATAGCCGCATTGCCAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCGCGCCCCATGGAATTAGAGGCGCGCt > 8:30678/1‑135 (MQ=255) gtTCCTGCCGGCGATGTATTCCATCTGGTTAAAGATACTCAACAATAGCAGCATTGCCAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCAACCCGTGGGCGGCCGCGGGCAATCGAGgcgcgcg > 2:320507/1‑134 (MQ=255) gCGATGTATTCCATCTGGTTCAAGATCCGCCCCAATAGCCGCATTGCCAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCGCGCCCGATGCAATTCGAGGCGCGCTCATGATCCAAc < 7:30678/136‑1 (MQ=255) tGTATTCCATCTGGTTCAAGATCCGCCCCAATAGCCGCATTGCCAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCGCGCCCGATGCAATTCGAGGCGCGCTCATGATCCAACgaga < 8:385076/136‑1 (MQ=255) tATTCCATCTGGTTCAAGATCCGCCCCAATAGCCGCATTGCCAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCGCGCCCGATGCAATTCGAGGCGCGCTCATGATCCAACGAGAgg < 4:333687/136‑1 (MQ=255) ccATCTGGTTCAAGATCCGCCCCAATAGCCGCATTGCCAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCGCGCCCGATGCAATTCGAGGCGCGCTCATGATCCAACGAGAGGTCg < 3:230029/135‑1 (MQ=255) cATCTGGTTCAAGATCCGCCCCAATTGCCGCATTGCCAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCGCGCCCGATGCTATTCGAGGCGCGCTCATGATCCAACGAGAGGTCGaa < 7:77419/136‑1 (MQ=255) ttCAAGATCCGCCCCAATAGCCGCATTGCCAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCGCGCCCGATGCAATTCGAGGCGCGCTCATGATCCAACGAGAGGTCGAAATGAc < 1:29264/134‑1 (MQ=255) ttCAAGATCCGCCCCAATAGCCGCATTGCCAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCGCGCCCGATGCAATTCGAGGCGCGCTCATGATCCAACGAGAGGTCGAAATGAc < 1:47788/134‑1 (MQ=255) aGATCCGCCCCAATAGCCGCATTGCCAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCGCGCCCGATGCAATTCGAGGCGCGCTCATGATCCAACGAGAGGTCGAAATGACTcaca < 5:68777/135‑1 (MQ=255) aGATCCGCCCCAATAGCCGCATTGCCAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCGCGCCCGATGCAATTCGAGGCGCGCTCATGATCCAACGAGAGGTCGAAATGACTcaca < 6:269258/135‑1 (MQ=255) gCCGCATTGCCAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCGCGCCCGATGCAATTCGAGGCGCGCTCATGATCCAACGAGAGGTCGAAATGACTCACAACGTTAATCAAAAgc < 4:355853/135‑1 (MQ=255) ccGCATTGCCAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCGCGCCCGATGCAATTCGAGGCGCGCTCATGATCCAACGAGAGGTCGAAATGACTCACAACGTTAATCAAAAgc < 6:93776/134‑1 (MQ=255) ccGCATTGCCAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCGCGCCCGATGAAATTCGAGGCGCGCTCATGATCCAACGAGAGGTCGAAATGACTCACAACGTTAATCAAAAgc < 1:83642/134‑1 (MQ=255) aTTGCCAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCGCGCCCGCTGCAATTCGAGGCGCGCTAATGATGCAACGAGAGGTCGAAATGACTCACAACCTTAATCAAAATCGCATc > 6:166009/1‑135 (MQ=255) gCCAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCGCGCCCGATGCAATTAGAGGCGCGCGCATGATCCAACGAGACGTCGCAATGACTCACAACGTTAATCAAAAGCGCATCgtg > 2:32848/1‑135 (MQ=255) acAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCGCGCCCGAAGCAATTCGAGTCGCGCTCATGATACAAAGAGAGGGCGAAATGTCACACAACGTTAATCAAAAGCGCATCgtggt > 7:215216/2‑136 (MQ=255) cAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCGCGCCCGATGCAATTCGAGGCGCGCTCATGATCCAACGAGAGGTCGAAATGACTCACAACGTTAATCAAAAGCGCATCgtggt < 1:320507/135‑1 (MQ=255) cAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCACCCCGGGCGCGCCCGATGCAATTCGAGGCGCGCTCATGATCCAACGAGAGGTCGAAATGACTCACAACGTTAATCAAAAGCGCATCGTGGTGaccg < 5:257414/135‑1 (MQ=255) gACCGCGCCTGAGTAGACATTGCCTTCAACCCGGGCGCGCCCGATGCAAATCGAGGCGCGCTCATGATCCAACGAGAGGTCGAAATGACTCTCAACGTTAATCAAAAGCGCATCGTGGTGACCGGCACCGGCGTTg < 8:327353/136‑1 (MQ=255) aCCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCGCTCCCGATGCAATTCGAGGCGCGCTCATGATCCAACGAGAGGTAGAAATGACTAACAACGTTAATCAAAAGCGCATCGTGGTGACCGGCACCGGCTTTg < 2:47777/135‑1 (MQ=255) aCCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCGCGCCCGATGCAATTCGAGGCGCGCTCATGATCCAACGAGAGGTCGAAATGACTCACAACGTTAATCAAAAGCGCATCGTGGTGACCGGCACCGGCGTTg < 5:12893/135‑1 (MQ=255) cgcCTGAGTAGACATTGCCTTCAACCCGTGCGCGCCCGATGCAATTCGAGGCGCGCTCATGATCCAACGAGAGGTCGAAATGACTCACAAAGGTAATCAAAAGCGCATAGTGGTGACCGGCACCGGCGGTGTCAGc > 4:92515/1‑136 (MQ=255) ttGCCTTCAACCCGTGCGCCCCCCCAGCAAAACGAGGCGCGGCCAAGAACCAACCAGGCGGAGCAATGCCTCAAAAAGTTAAACAAAACCCCATAGTTGTGCCCCGCCCCCGCGTTGTCACCCCGCCGggcggccg > 4:350589/1‑134 (MQ=255) gCCTTCAACCCGTGCGCGCCCGATGCAATTAGAGGCGCGCTCATGTTACAACGAGAGGGCGCAATTACTCACAACGGTTATCAAAAGCGCATCGTGGTGGCCGGCCCCGGCGTTGTAACCCCCCTGGGCTggggc > 6:272903/1‑135 (MQ=255) ttCAACCCGTGCGCGCCCGATGCAATTCGAGGCGCGCTCATGATCCAACGAGAGGTCGAAATGACTCACAACGTTAATCAAAAGCGCATCGTGGTGACCGGCCCCGGCGTTTTCAGCCCGCGGGGCGGCGGCGTa > 4:279062/1‑135 (MQ=255) cgcgcCCGATGCAAATCGAGGCGCGCTCATGATCCAACGAGAGGTCGAAATGACTCACAACGTTAATCAAAAGCGCCTCGTGGTGACCGGCACCGGCGTTGTCAGCCCGCTGGGCTGCGGCGTAAAGGAAGTCTgg < 8:283963/136‑1 (MQ=255) gcCCGATGCAATTCGAGGCGCGCTCATGATCCAACGAGAGGTCGAAATGACTCACAACGTTAATCAAAAGCGCATCGTGGTGACCGGCACCGGCGTTGTCAGCCCGCTGGGCTGCGGCGTAAAGGAAGTCTggcg < 6:339338/135‑1 (MQ=255) aTGCAATTCGAGGCGCGCTCATGATCCAACGAGAGGTCGAAATGACTCACAACGTTAATCAAAAGCGCATCGTGGTGACCGGCACCGGCGTTGTCAGCCCGCTGGGCTGCGGCGTAAAGGAAGTCTGGCGGCGAt < 5:272903/135‑1 (MQ=255) aaTTCGAGGCGCGCTCATGATCCAACGAGAGGTCGAAATGACTCACAACGTTAATCAAAAGCGCATCGTGGTGACCGGCACCGGCGTTGTAAGCCCGCTGGGCTGCGGCGTAAAGGAAGTCTGGCGGCGATTgctg > 8:258892/1‑136 (MQ=255) | CCTTGGTGTTCCTGCCGGCGATGTATTCCATCTGGTTCAAGATCCGCCCCAATAGCCGCATTGCCAAGCCAGAAGCTGCAGACCGCGCCTGAGTAGACATTGCCTTCAACCCGTGCGCGCCCGATGCAATTCGAGGCGCGCTCATGATCCAACGAGAGGTCGAAATGACTCACAACGTTAATCAAAAGCGCATCGTGGTGACCGGCACCGGCGTTGTCAGCCCGCTGGGCTGCGGCGTAAAGGAAGTCTGGCGGCGATTGCTG > ACB122/3731162‑3731424 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |