Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,879,284 A→G 6.9% E508E (GAA→GAG)  rcsC_8 → Sensor histidine kinase RcsC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,879,2840AG 6.9% 176.9 / 3.6 58E508E (GAA→GAG) rcsC_8Sensor histidine kinase RcsC
Reads supporting (aligned to +/- strand):  ref base A (24/30);  new base G (2/2);  total (26/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

GATTTGCTCATCACCGATGTGGGCTTGCCCGGCATGAACGGCCGACAACTGGCAGAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTAGGTAAGCCTTGAAGCGCCTTACACGTCAAGCAGGCGTAGCGCCTCATCGG  >  ACB122/3879153‑3879415
                                                                                                                                   |                                                                                                                                   
gATTTGCTCATCACCGATGTGGGCTTGCCCGGCATGAACGGCCGACAACTGGCTGAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGc                                                                                                                                  >  1:274343/1‑135 (MQ=255)
gATTTGCTCATCACCGATGTGGGCTTGCCCGGCATGAACGGCCGACAACTGGCAGAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCt                                                                                                                                 >  8:39838/1‑136 (MQ=255)
  tttGCTCATCACCGATGTGGGCTTGCCCGGCATGAACGGCCGACAACTGGCAGAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCtt                                                                                                                                <  1:186453/135‑1 (MQ=255)
  tttGCTCATCACCGATGTGGGCTTGCCCGGCATGAACGGCCGACAACTGGCAGAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCtt                                                                                                                                <  2:58874/135‑1 (MQ=255)
      cTCATCACCGATGTGGGCTTGCCCGGCATGAACGGCCGACAACTGGCAGAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTgg                                                                                                                           <  8:191947/136‑1 (MQ=255)
       tcatcaCCGATGTGGGCTTGCCCGGCATGAACGGCCGACAACTGGCAGAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAGGGCTTCCTggg                                                                                                                          >  4:241604/1‑136 (MQ=255)
         atcaCCGATGTGGGCTTGCCCGGCATGAACGGCCGACAACTGGCAGAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTggg                                                                                                                          <  8:197148/134‑1 (MQ=255)
         atcaCCGATGTGGGCTTGCCCGGCATGAACGGCCGACAACTGGCAGAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTggg                                                                                                                          <  3:267213/134‑1 (MQ=255)
             cggATGGGGGCTGGCCCGGCATGAACGGCCGCCCACTGGCAGAAATTCCCCGCAGCCTGCGCCCACGATTAGCGGGGCTGTTCATCACCGGTTACGCGGACACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTg                                                                                                                     <  2:190397/133‑1 (MQ=255)
              cGATGGGGGCTTGCCCGGCATGAACGGCCGACAACTGGCAGAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGAc                                                                                                                   <  7:172105/136‑1 (MQ=255)
                   tgGGCTTGCCCGGCATGAACGGCCGACAACTGGCAGAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGc                                                                                                               >  5:186520/1‑135 (MQ=255)
                        ttGCCCGGCATGAACGGCCGACAACTGGCAGAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGa                                                                                                         >  3:380856/1‑136 (MQ=255)
                             cGGCATGAACGGCCGACAACTGGCAGAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCt                                                                                                      >  6:72644/1‑134 (MQ=255)
                             cGGCATGAACGGCCGACAACTGGCAGAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCt                                                                                                      >  6:325310/1‑134 (MQ=255)
                                           gACAACTGGCAGAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGtt                                                                                      >  4:350116/1‑136 (MQ=255)
                                            aCAACTGGCAGAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTg                                                                                     >  4:216425/1‑136 (MQ=255)
                                            aCAACTGGCAGAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTg                                                                                     <  7:248123/136‑1 (MQ=255)
                                             caccTGGCAGAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGACACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTg                                                                                     <  2:374675/132‑1 (MQ=255)
                                             cAACTGGCAGAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTg                                                                                     <  1:264136/135‑1 (MQ=255)
                                              aaCTTGCAGAAATTCCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACGGGTTACGCGGTAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTgc                                                                                    <  5:350551/135‑1 (MQ=255)
                                              aaCTGGCAGAAATTGCCCTCAGCCTGCGACCACGATTACCGGTGCTGTTCATCAACGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTgc                                                                                    <  6:93135/135‑1 (MQ=255)
                                              aaCTGGCAGAAATTGACAGCAGCCTGCGACCAAGATTCTGGGTGCTGTTCATCACCGGTTAAGAGTAAACGGCCATGGGCCGCGAAGTCTTCCTGGGCGCTGACATGCACCTGATCTTCAAGCCGTTCGAGTTgc                                                                                    <  6:87485/135‑1 (MQ=255)
                                                   gCAGAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGcagcag                                                                               <  2:229515/135‑1 (MQ=255)
                                                      gAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTg                                                                            >  1:315851/1‑135 (MQ=255)
                                                      gAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTg                                                                            >  1:302474/1‑135 (MQ=255)
                                                             cccGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCg                                                                    <  3:382160/136‑1 (MQ=255)
                                                              ccGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCg                                                                    <  5:16871/135‑1 (MQ=255)
                                                                   gCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGac                                                              <  7:43488/136‑1 (MQ=255)
                                                                    ccTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCTGAAACGGCCATGGACCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCAGTTCGAGTTGCAGAAGTTGCAGGCTCGAGTGac                                                              <  6:209146/135‑1 (MQ=255)
                                                                          cccACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGATTTGCAGCAGTTGCAGGCTCGAGTGCCACACAt                                                        >  5:12401/1‑135 (MQ=255)
                                                                          cccACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACAt                                                        >  1:334761/1‑135 (MQ=255)
                                                                           ccACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAACCGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTc                                                      >  3:373729/1‑136 (MQ=255)
                                                                             aCGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAGGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTa                                                    <  3:241604/136‑1 (MQ=255)
                                                                             aCGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTa                                                    <  8:176252/136‑1 (MQ=255)
                                                                              cGATTACCGGTGCTGTTCATCACCGGTTACGCGGACACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTa                                                    <  5:325967/135‑1 (MQ=255)
                                                                                 ttACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTAGGt                                                 >  3:389787/1‑135 (MQ=255)
                                                                                       gTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTAGGTAAGCCtt                                          <  3:90990/136‑1 (MQ=255)
                                                                                        tGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTAGGTAAGCCtt                                          <  2:274343/135‑1 (MQ=255)
                                                                                            gTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGCCATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACAGTCTAGGTAAGCCTTGAAgc                                     >  4:252355/1‑136 (MQ=255)
                                                                                            gTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTAGGTAAGCCtt                                          <  7:132071/131‑1 (MQ=255)
                                                                                            gTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTAGGTAAGCCtt                                          >  8:132071/1‑131 (MQ=255)
                                                                                            gTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTAGGTAAGCCTTGAAg                                      >  1:171328/1‑135 (MQ=255)
                                                                                                atcaCCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTAGGTAAGCCTTGAAGCGcc                                  >  6:179593/1‑135 (MQ=255)
                                                                                                    ccGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTAGGTAAGCCTTGAAGCGCCTTaca                             <  3:43616/136‑1 (MQ=255)
                                                                                                       gTTACGCGGAAACGGCCATGGCCCGCGAGGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTAGGTAAGCCtt                                          <  1:201890/120‑1 (MQ=255)
                                                                                                       gTTACGCGGAAACGGCCATGGCCCGCGAGGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTAGGTAAGCCtt                                          >  2:201890/1‑120 (MQ=255)
                                                                                                        ttACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTAGGTAAGCCTTGAAGCGCCTTACACGTc                         <  4:182087/136‑1 (MQ=255)
                                                                                                         tACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTAGGTAAGCCTTGAAGCGCCTTACACGTc                         <  2:293792/135‑1 (MQ=255)
                                                                                                         tACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTAGGTAAGCCTTGAAGCGCCTTACACGTc                         <  2:176868/135‑1 (MQ=255)
                                                                                                          aCGCGGCTACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCACGCTCGAGTGCCACACATTCGAGGTCAGCCTTGCAGCGCCTTACACGTcc                        >  6:56947/1‑134 (MQ=255)
                                                                                                          aCGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGTCTCGAGTGACACACATTCTAGGTAAGCCTTGAAGCGCCTTACACGTCa                        <  4:161617/135‑1 (MQ=255)
                                                                                                          aCGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTAGGTAAGCCTTGAAGCGcc                                  <  4:304142/125‑1 (MQ=255)
                                                                                                          aCGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTAGGTAAGCCTTGAAGCGcc                                  >  3:304142/1‑125 (MQ=255)
                                                                                                          aCGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTAGGTAAGCCTTGAAGCGCCTTACACGt                          >  1:30145/1‑133 (MQ=255)
                                                                                                          aCGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTAGGTAAGCCTTGAAGCGCCTTACACGTc                         >  7:272765/1‑134 (MQ=255)
                                                                                                           cgcgGAAACCGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACAAGCACCTGATCTGCAATCCGATCGAGTTGCAGCAGTTGCAGGCTCGAGTGACAAACATTCTATGTAAGCCTTGAAGCGCATTACACGTCa                        <  5:363566/134‑1 (MQ=255)
                                                                                                            gcgGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTAGGTAAGCCTTGAAGCGCCTTACACGTCAAg                      >  8:119458/1‑135 (MQ=255)
                                                                                                                        aTGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGCCACACATTCTAGGTAAGCCTTGAAGCGCCTTACACGTCAAGCAGGCGTAGCGcc         >  8:181865/1‑136 (MQ=255)
                                                                                                                                   aGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTAGGTAAGCCTTGAAGCGCCTTACACGTCAAGCAGGCGTAGCGCCTCATCgg  >  5:118420/1‑132 (MQ=255)
                                                                                                                                   aGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACAGTCTAGGTAAGCCTTGAAGCGCCTTACACGTCAAGCAGGCGTAGCGCCTCATCgg  >  1:225423/1‑132 (MQ=255)
                                                                                                                                   |                                                                                                                                   
GATTTGCTCATCACCGATGTGGGCTTGCCCGGCATGAACGGCCGACAACTGGCAGAAATTGCCCGCAGCCTGCGCCCACGATTACCGGTGCTGTTCATCACCGGTTACGCGGAAACGGCCATGGCCCGCGAAGGCTTCCTGGGCGCTGACATGCACCTGATCTGCAAGCCGTTCGAGTTGCAGCAGTTGCAGGCTCGAGTGACACACATTCTAGGTAAGCCTTGAAGCGCCTTACACGTCAAGCAGGCGTAGCGCCTCATCGG  >  ACB122/3879153‑3879415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑