| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 4,134,647 | T→G | 17.0% | intergenic (+33/‑36) | CJ019_03892 → / → tgnD | hypothetical protein/(E)‑2‑((N‑methylformamido)methylene)succinatehydr olase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 4,134,647 | 0 | T | G | 17.0% | 118.3 / 2.3 | 64 | intergenic (+33/‑36) | CJ019_03892/tgnD | hypothetical protein/(E)‑2‑((N‑methylformamido)methylene)succinatehydr olase |
| Reads supporting (aligned to +/- strand): ref base T (16/34); new base G (11/2); total (28/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.07e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTCCTGGGCGCAGCCGATGGCGGCCGCCTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTG > ACB122/4134515‑4134772 | gggccTGGGCGCAGCCGATGGCGGCCGCCGGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCGCAATAACAACACCCAAGACGGGCCGGGGCGTGGCGTGCTTAc < 8:273828/133‑1 (MQ=255) gcAGCCGCTGGCGGCCGCCTGCACCCGCGCATCGGCCACCGTTACATCGACCGGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAAGAACACCAACCCAGACGGGCCGGGGCGTGGCGGGCTTGCCCGCCGCGcc > 8:313538/1‑136 (MQ=255) gcAGCCGATGGCGGCCGCCTGCACCCGAGCATCGGCAACCGTTAAATCGACCTGGAAGAACATGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCCGGGCGTGGCGTTCTTACCCGCCgcgc > 6:108832/1‑135 (MQ=255) aGCCGATGGCGGCCGCCTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACCACCAAGACGGGCCGGGGCGTGGCGTGGTTTCCCGCCGCGgcc > 1:216062/1‑135 (MQ=255) cGATGGCGGCCGCCTGCACCCGCGCATCGGCAACCGTTACATCGCCCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCCGGGCCGGCGTTCCCCCCCCGccccccc > 7:59958/1‑132 (MQ=255) cGATGGCGGCCGCCTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACCACAACCAAGACGGGCCGGGGCGTGGCGGGCGTACCCGCCGCgcccgccc > 4:74143/1‑136 (MQ=255) cGATGGCGGCCGCCTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGGGGGGGTTCCCCCCGCGCCCgcc > 5:391399/1‑133 (MQ=255) cGATGGCGGCCGCCTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAACCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGGGCGTACCcgccccgccccccc > 8:12472/1‑132 (MQ=255) cGATGGCGGCCGCCTGCACCCGCGCATCGGCAACCGTTACATCGACCGGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCgcccgccc > 4:173732/1‑136 (MQ=255) cGATGGCGGCCGCCTGCACCCGCGCATCGGCAACCGTTACATAGACCTGGCAGAACTGGCTGGCGAAAAAGCCAACGGTCCATAACAACAACCAAGACGGGCCGGGGCGTGGGGTTGGTTCCCCCCGCGccccgcc > 8:72770/1‑132 (MQ=255) aTGGCGGCCGCCTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCGTACCCGCCGCgcccgccc > 2:260998/1‑134 (MQ=255) gcggcCGCCTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCaa < 7:329476/136‑1 (MQ=255) cggcCGCCTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGATCTGGCTGCGGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAg < 7:67505/136‑1 (MQ=255) ggcCGCCTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGGGTGCGTACCCGCCCCGCCCCGCCGTCaaag > 3:68962/1‑134 (MQ=255) gccgccTGCACCCGCGCATCGGCAACCGTTACTTCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGa < 8:373957/136‑1 (MQ=255) cgccTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGGGTGCTTACCCGCCGCGGCCGCCCGTCAAGgcgc > 3:260462/1‑136 (MQ=255) cgccTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAgc < 8:298048/136‑1 (MQ=255) ccTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGCCCTTCAAGGAgcgc > 4:291037/1‑136 (MQ=255) cACCCGCGCATCGGAAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAACCCAACGCTCTATAACAACAACCTCGACGGTCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGTAGCGccc < 4:110763/134‑1 (MQ=255) cATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGAtt > 8:135139/1‑135 (MQ=255) aTCGGCAACCGTTACATCGCCCTGGACGCCCGGGCGGCCGAAAAAGCCAACGCTCAATAACACCAACCACGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTc < 1:41479/135‑1 (MQ=255) cGGCAACCGTTACATCGCCCTGGAAGAACTGGCGGCCGAAAACGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGc < 8:314403/136‑1 (MQ=255) gCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGCCCGTCAAGGCGCGCCCGCCCTGCTTCCGCCt > 3:100143/1‑136 (MQ=255) cAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGGGCTTACCCCCCGCGCCCCCCCGTCAAGGACCGGCCACCATGGTTCAGccag > 4:257455/1‑134 (MQ=255) ccGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTc < 2:274251/135‑1 (MQ=255) ccGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTc < 2:274249/135‑1 (MQ=255) tCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGc < 4:245657/136‑1 (MQ=255) gcccTGCAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCCACACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGca < 6:338743/133‑1 (MQ=255) gACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGcac > 4:140796/1‑136 (MQ=255) ccTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCacac < 3:140796/136‑1 (MQ=255) tcgaagaaCTGGCTGCCCAAAAAGCCAACGCTCAATAACAACAACCACGACGGGCCGCGGCCTGCCGTTGATACCCCCCGCGCCCGACCATACATGAACGCACACCATGATTAAGACTATCCCAGAACCCACAcca > 4:155717/3‑135 (MQ=255) tGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTTGCGAGCTTACCCGCCCCGCCCGACCTTAAAGCAGCCCCCCACAAGAATCAACCAGTCACAGAACGccca > 2:204934/1‑133 (MQ=255) ctgcctgcCCAAAACCCCAACGCTCAAAAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCAc < 3:234543/136‑1 (MQ=255) tGGCTCCCTAAAAAGCCAACGCTCAAACACAACAACCACGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCAc < 1:147918/135‑1 (MQ=255) gCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGCCCTTCAAGGCGCGCCCTCCCTGATTAAGCCTGTCGCTGGACGCCCCCCGGCCGGCACCac > 7:385332/1‑135 (MQ=255) gCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGGGTTCCCCCCCCGCCCCCCCGTCAACGCGCGGCCGCCCAGATTAAGCCTGTCGCGGCAAGCCCCCCAGCCGGCACCCGcga > 3:348211/1‑134 (MQ=255) ccGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGGGCGTAACCCCCCCCCCCCCCCGTCAACGACCCCCCACCAAGATAAAGCCGGTCGCAGAACCCACACCAGCCCGCACCAGcca > 4:114674/1‑133 (MQ=255) aaaaGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTgg < 8:260329/136‑1 (MQ=255) aaaGCCAACGCTCAATAACAACAACCACGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTgg < 2:38930/135‑1 (MQ=255) ccAACGCTCAATAACAACAACCAAGACTGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTc < 8:118342/136‑1 (MQ=255) cAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTc < 6:311016/135‑1 (MQ=255) cAATACCAACACCCACGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAggg < 3:226031/136‑1 (MQ=255) ccaacaccCAAGACGGGCCGGGGCGTGGCGTGCTGACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAAc < 8:74516/135‑1 (MQ=255) ccaacaacCCCGCCGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCGGGATGTCGGCCAGGGCCAAc < 7:12472/135‑1 (MQ=255) caacaacCACGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAAc < 2:159137/135‑1 (MQ=255) aaccacccACGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCCTGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAAc < 6:24414/129‑1 (MQ=255) aacCACGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCgtg < 4:348237/136‑1 (MQ=255) ccAAGACGGGCCGGGGCGTGGCGTGCGTACCCGCCCCGCCCGACCTTCAAGGAGCGCCCTACAAGATTCATCCAGTCGCTGTACCCCCCCCACCCCGCCCCCGCTATCTGGATTTCGGCCAGGGCCAACCCctgg > 2:332387/1‑135 (MQ=255) aaGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCGATATGGATGTCGGCCAGGGCCAACCCCTGGTGc > 7:17956/1‑136 (MQ=255) cGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTGCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGAt < 3:257455/136‑1 (MQ=255) cGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGAt < 7:186612/136‑1 (MQ=255) cGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGAt < 7:87817/136‑1 (MQ=255) gggCCGGGGCGTGGCGGGAGCACCCCCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGAt < 5:232113/135‑1 (MQ=255) ggCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCCCCCCTGCCGGCACCAGCGATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATc > 3:94609/1‑135 (MQ=255) ggCCGGGGCGTGGCGTGCTTACCCGCCCCGCCCGCCCATCAACGAGCCCCCTCCCTGATTCAGCCAGTCGCTGCACGCCCACCAGCCGGCCCCCGCGATCTGGATGTCGGCCAGGGCCCACCCGTGGTGCTGCTcc > 8:52212/1‑136 (MQ=255) cGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACg < 7:46189/136‑1 (MQ=255) cGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACg < 7:135139/136‑1 (MQ=255) cGGGGCGTGGCGTGCGTACCCGCCGCGCCCGCCCTTCAAGGCGCGCCCGCCAAGATTAAGCCGGTAGCGGGACGCACACCAGCCGGCACCAGCTATCTGGTTGTCGGCCACGGCCCACCCGTGGGGCGGGTCCAc > 2:318986/1‑135 (MQ=255) ggggCGTGGCGTGCTTCCCCGCCGCGCCCGACCTTCCAGGAGCGCCCTCCATGAATCAGCCTCGCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACg < 5:252394/135‑1 (MQ=255) ggggCGTGGCGTGCGTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCAGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACg < 1:396861/135‑1 (MQ=255) ggcgtggcgtGCTTACCCGCCGCGCCCGGCCGTCAAGGCGCGCCCGCCCTGATTCAGCCTGTCGCGGAACGCCCCCCTGCCGGCACCCGCGATCTGGGTGTCGGCCAGGGCCAACCCCTGGTGCTGCTCCCCGGCg > 8:186612/1‑136 (MQ=255) cgtggcgtgCTTACCCGCCGCGCCCGACCTGCACGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTg < 3:56307/136‑1 (MQ=255) gtggcgtgCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTg < 1:214117/135‑1 (MQ=255) gtggcgggCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACGAGCTATCTGGATGTCGGCCAGGGCCACCCCGTGGTGCTGATCCACGGCGTg < 6:264623/135‑1 (MQ=255) | GGTCCTGGGCGCAGCCGATGGCGGCCGCCTGCACCCGCGCATCGGCAACCGTTACATCGACCTGGAAGAACTGGCTGCCGAAAAAGCCAACGCTCAATAACAACAACCAAGACGGGCCGGGGCGTGGCGTGCTTACCCGCCGCGCCCGACCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTG > ACB122/4134515‑4134772 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |