| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 4,789,585 | A→C | 26.5% | V230G (GTG→GGG) | dat ← | Diaminobutyrate‑‑2‑oxoglutarateaminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 4,789,585 | 0 | A | C | 26.5% | 48.8 / 2.0 | 50 | V230G (GTG→GGG) | dat | Diaminobutyrate‑‑2‑oxoglutarateaminotransferase |
| Reads supporting (aligned to +/- strand): ref base A (24/11); new base C (13/2); total (37/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCG‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGAGCA > ACB122/4789451‑4789717 | ggATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCG‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCCGCGGCAc > 3:36240/1‑136 (MQ=255) cgccgaGCAACGCCACGCCCGCCGGCTCGGTAATGCGCCGCACGCCCATGAGCCATTCG‑ATGTCGGCCGGACCCCCCCCGCCCTCACCCTGCACCACTTCGAGGATCACGCCGGCGGGCAGCGGCACCCCGCgtt > 5:141059/4‑135 (MQ=255) cgacgaTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCG‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCtgt > 1:205286/1‑133 (MQ=255) gccacgccCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCG‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCCGCGGCCCGCCGCGTTCCggctgga > 1:101915/1‑128 (MQ=255) ccgccTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCG‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCTTTCCGGATCGAGCAGCAggg > 1:44771/1‑134 (MQ=255) gCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCG‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCCGCGGCCCGCCGCGTTCCGGATCGGGCAGCAGGTTTTCGAg > 3:234015/1‑136 (MQ=255) cGGTAATGCGCCGCCCGCCTTTG‑TCCATTGGTTTGTCGGCGGTAACCCCCCCCCCTCCACCCTGCACCCCTGCGCGACCCCGGGGGGGGGGCTGCGGACCGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTa < 4:65214/136‑1 (MQ=255) ctGCGCCGCACGCCCTTGAGCCATTCG‑ATGGCGGCCGTAACCACCCCGCCCTCCCCCTCCCCCACTTCGTGGACCCCGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCa < 4:270801/135‑1 (MQ=255) cgcACGCCCTTGAGCCATTCG‑ATGTCGGCCGGAACCACCCCGCCCTCGCCCTGCACCACTTCGAGGATCACGGCGCCGGGCCCCGGCACCCCGCCGTCCGGAACGAGCCGCAGGGTTTCCAGGTAATGCCGGTTgg > 3:180349/1‑136 (MQ=255) cgcACGCCCTTGAGCCATTCG‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCCCCCGCCCCCCCCGGTGCGGGTAGCTCAGCCGGGGTTGCAGGTAATGCAGGTTg > 5:61365/1‑135 (MQ=255) gCCCTTGAGCCATTCG‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCGTTCCGGATCGAGCAGCAGGTTTTCCAGGGAATGCAGGTTGGCCTTg > 3:270801/1‑136 (MQ=255) gCCATTCG‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGCCCAGCAGGTTTTCCAGGGAATGCAGGTTGGCCTTGACCCCGGc > 8:24175/1‑136 (MQ=255) g‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTTGAGGATCACGGCGGCGGGCAGCGGCACGCCCCGTTCCGGATAGAGCAGCCGCTTTTACAGGTAATGCAGGTTGGCCTTGACCCCGGCCTAAcc > 5:342676/1‑135 (MQ=255) g‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGGAGCGGCCCGCCGCTTTCCGGAGCGCGCAGCAGGGTTTGCAGGTAATGCAGGTTGGCCTTGCCCCCGGCCTCCCCg > 3:239598/1‑136 (MQ=255) g‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCCGCGGCCCGCCGCTTTCCGGATCGAGCAGCAGGGTTTGCAGGTAATGCAGGTTGTCCGTGCCCCCGGCCGCAcc > 2:289983/1‑135 (MQ=255) g‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCCGCGGCCCCCCGCGTTCCGGGTCGAGCCGCCGGGTTTGCAGGGTATGCAGGTTTGCCTTGGCCCCCGCCTCCCCg > 7:153337/1‑136 (MQ=255) g‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCCGCCGCCCGCCCCGTTGCCGCTCGAGCACCCGGTTTTACAGGTAATTCAGGTTGGCCGTGACCCCCGCCACAcc > 5:382792/1‑135 (MQ=255) g‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCGTTCCGGATCGCGCAGCAGGTTTTCCAGGTAATGCAGGTTGGGCTTGACCCCGGCCTCAcc > 2:17004/1‑135 (MQ=255) g‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTTCAGGTAATGCAGGTTGGCCTTTAACCCGGCCTCAcc > 6:139190/1‑135 (MQ=255) g‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGCCCCCGGCCTCACCg > 8:118408/1‑136 (MQ=255) g‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCg > 8:333636/1‑136 (MQ=255) g‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCGTTCCGGCTCGCGCAGCAGGTTTTGCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCAccc > 3:291251/1‑135 (MQ=255) g‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCGTTCCGGCTCGAGCCGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCAcc > 6:225127/1‑135 (MQ=255) g‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCGTTCCGGATAGTGCAGCAGGTTTTGCAGGGAATTGAGGTTGGCCTTGACCCCGGCCGCACCg > 3:139590/1‑136 (MQ=255) g‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACCCCGCGTTCCGGATCCAGCCGCAGGTTTTCCAGGTAATGGAGGTTGGCCTTGCCCCCGGCCTCAcc > 2:10151/1‑135 (MQ=255) g‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCACCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCGTGACCCCGGCCTCACCg > 7:281354/1‑136 (MQ=255) ggAACCACCCCGCCCTCACCCTGCACAACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTGTCCGGAGCGCGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGa > 7:263573/1‑136 (MQ=255) ccccccGGCACCACTCCGAGGCCCAGGGCGGCGGGCCGCGGCACGCCGCGTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAg < 2:274801/132‑1 (MQ=255) aaCCCTGCACCACTTCGAGTATAAGGGGGGGGGGAGCCGGCCCGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTc < 7:24175/135‑1 (MQ=255) aCCCTGCCCCACTGCGCGGAGCCCGGCGGCGGGCCGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTc < 2:305818/135‑1 (MQ=255) aCCCTGCACCACTTGGTGGAGCCCGGGGGCGGGCTGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTc < 6:382792/135‑1 (MQ=255) aCCCTGCACCACTTCGAGGATAACGGCGGGGGGCTGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTc < 6:324427/135‑1 (MQ=255) cTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCGTTCCGGCTCGAGCAGCAGGTTTTGCAGGTAATGCAGGTTGGCCTTGACCCCGGCCGCCCCGCCCAGGCCGCACGGGCAGCGGTAGTCGTa > 6:278031/1‑135 (MQ=255) ggCACCCCTTCGAGCAGCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACg < 8:230497/135‑1 (MQ=255) ccccagTTCGTGTAGCAGGGGGGGGGGCAGCGGCAGGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACg < 3:91340/132‑1 (MQ=255) caccacTTCGAGCATCCCGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACg < 3:100616/134‑1 (MQ=255) accacTTCGTGGATCAGGGGGGCGGGCAGGGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACg < 6:378988/133‑1 (MQ=255) cacTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCTTTCCGGATCGAGCAGCAGGTTTTACAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCACGCCGAACGGGCCGCGGTAGTCGTACGGGt > 7:316549/1‑134 (MQ=255) cacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACggg > 5:322100/1‑133 (MQ=255) cacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTAAggg > 2:227051/1‑133 (MQ=255) cacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCACGCCGAACGGGCAGCGGTAGTCGGACGGGt > 7:84570/1‑134 (MQ=255) cacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGGAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGt > 7:134226/1‑134 (MQ=255) cGAGGATCACGGCGGCGGGCAGCGGCCCCCCGCGTTACGGCTAGAGCAGCAGGGTTTGCAGGTAATTCATGTTTGCCTTTACCCCCGCCGCACCGCCCCGGCCCCACCGGCACCGGTAGTAGTACGGGTAAGGCa > 6:32705/1‑135 (MQ=255) cGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCAt > 4:331431/1‑136 (MQ=255) gAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTGCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTTGCCTTGACCCCGGCCTCCCCGCCCAGGCCGCACGGGCAGCGGTAGTCGGACGGGTAAGGCa > 5:249645/1‑134 (MQ=255) gAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCCCCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCAt > 3:84779/1‑135 (MQ=255) ggATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGCGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGaa > 7:38162/1‑136 (MQ=255) ggATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGa > 6:86399/1‑135 (MQ=255) tcccggcggcggGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACt < 2:44771/132‑1 (MQ=255) ggCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGAGCa < 8:219719/136‑1 (MQ=255) | GGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCG‑ATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGAGCA > ACB122/4789451‑4789717 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |