Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,264,429 C→T 5.9% intergenic (‑123/‑54) radA ← / → mscL DNA repair protein RadA/Large‑conductance mechanosensitive channel

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,264,4290CT 5.9% 222.6 / 3.1 68intergenic (‑123/‑54)radA/mscLDNA repair protein RadA/Large‑conductance mechanosensitive channel
Reads supporting (aligned to +/- strand):  ref base C (32/32);  new base T (2/2);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.62e-01

GGCCTTGGCCATGGGTGAGGTCTCCTGGAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCAACGAGTTCAAGGCTTTCGCAGTCAAAGGCAATGTCGTCGACATGGCGGTCGGTATCATCATCG  >  ACB122/5264295‑5264558
                                                                                                                                      |                                                                                                                                 
ggccttggccATGGGTGAGGTCTCCTGGAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGc                                                                                                                                   >  1:168266/1‑135 (MQ=255)
  ccttggccATGGGTGAGGTCTCCTGGAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGtt                                                                                                                                <  4:384120/136‑1 (MQ=255)
  ccttggccATGGGTGAGGTCTCCTGGAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGtt                                                                                                                                <  4:379382/136‑1 (MQ=255)
   cttggccATGGGTGAGGTCTCCTGGAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGtt                                                                                                                                <  5:389111/135‑1 (MQ=255)
       gccATGGGTGAGGTCTCCTGGAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGt                                                                                                                           <  8:353279/136‑1 (MQ=255)
       gccATGGGGGAGGTCTCCTGGAAAAAATCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGt                                                                                                                           <  8:154171/136‑1 (MQ=255)
         cATGGGTGAGGTCTCCTGGAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTg                                                                                                                          >  6:251953/1‑135 (MQ=255)
         cATGGGTGAGGTCTCCTGGAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTg                                                                                                                          >  6:142169/1‑135 (MQ=255)
             ggggAGGTCTCCTGGAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCtt                                                                                                                      <  1:389592/132‑1 (MQ=255)
             ggTGAGGTCTCCTGGAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCtt                                                                                                                      <  8:376901/135‑1 (MQ=255)
             ggTGAGGTCTCCTGGAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCtt                                                                                                                      <  7:81639/135‑1 (MQ=255)
             ggTGAGGTCTCCTGGAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCtt                                                                                                                      <  7:11285/135‑1 (MQ=255)
             ggTGAGGTCTCCTGGAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCtt                                                                                                                      <  4:339531/135‑1 (MQ=255)
              gTGAGGTCTCCTGGAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCtt                                                                                                                      <  5:43681/134‑1 (MQ=255)
              gTGAGGTCTCCTGGAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCtt                                                                                                                      <  5:13019/134‑1 (MQ=255)
               tGAGGTCTCCTGGAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGACGCGTTTAAGTGGCtttc                                                                                                                    >  1:382740/1‑133 (MQ=255)
               tGAGGTCTCCTGGAAAAATTAGCCATGATAGCTGAGCAGGACGTGTATAGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGACCTGAACACAACGCATGAAGCGTTTAAGTGGCTTaca                                                                                                                   >  7:327676/1‑136 (MQ=255)
                   gTCTCCTGGAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACGGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTg                                                                                                                <  3:115644/135‑1 (MQ=255)
                    tctcCTGGAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCtt                                                                                                                      <  6:124828/128‑1 (MQ=255)
                    tctcCTGGAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCtt                                                                                                                      >  5:124828/1‑128 (MQ=255)
                    tctcCTGGAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGc                                                                                                               <  6:136269/135‑1 (MQ=255)
                    tctcCTGGAAAAATTCGCCATGATAGCTGAGCAGGACGTGGCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGc                                                                                                               <  2:107885/135‑1 (MQ=255)
                          ggAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAac                                                                                                         >  1:274929/1‑135 (MQ=255)
                           gAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGACGCGTTTAAGTGGCTTACACTGCCTTAacc                                                                                                        >  5:37453/1‑135 (MQ=255)
                           gAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATCCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAacc                                                                                                        >  1:119572/1‑135 (MQ=255)
                              aaaTTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAaccttac                                                                                                    <  3:26817/136‑1 (MQ=255)
                               aaTTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAaccttac                                                                                                    <  5:251953/135‑1 (MQ=255)
                                  tCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAaccttacctt                                                                                                 <  6:296111/135‑1 (MQ=255)
                                   cGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCtt                                                                                                                      <  5:62796/113‑1 (MQ=255)
                                   cGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCtt                                                                                                                      >  6:62796/1‑113 (MQ=255)
                                            aGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAg                                                                                       <  2:226487/135‑1 (MQ=255)
                                                      gACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTATAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAAc                                                                              >  3:80227/1‑134 (MQ=255)
                                                         gtgtCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATgg                                                                          >  1:283967/1‑135 (MQ=255)
                                                              tccaaCGCCTAGGGATGCGACAAAACTATTAGTTCTAACTGCAGTCAGTAGCCCTCAACACAACGCATGAATCGTTTAAGAGGCTTACACAGCCATAACCTTACCTTTTGATACAATGAACCAAAAAAGGGAATg                                                                     >  6:352336/4‑135 (MQ=255)
                                                              tcGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGc                                                                    >  3:17343/1‑136 (MQ=255)
                                                              tcGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGc                                                                    >  7:2571/1‑136 (MQ=255)
                                                              tcGAACGCCTCGGGATGCGACAAAACTATTCGTACTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCtt                                                                                                                      >  4:322646/1‑86 (MQ=255)
                                                              tcGAACGCCTCGGGATGCGACAAAACTATTCGTACTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCtt                                                                                                                      <  3:322646/86‑1 (MQ=255)
                                                              tcGAACGCCTCGGGATGCGACAAAACAAATCGTTCTAACTGCACTCAGTAGCCCTGAACACAACGCATGAAGCCTTTAAGTTGCTTACACTGCCTTAACCATAACTTTTGTTACAAGGAACCAAACATGCGCATg                                                                     >  1:75618/1‑135 (MQ=255)
                                                                 aaCGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTc                                                                  <  5:177765/135‑1 (MQ=255)
                                                                 aaCGCCGCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCa                                                                 >  7:224679/1‑136 (MQ=255)
                                                                   cGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCaa                                                                >  5:187471/1‑135 (MQ=255)
                                                                   cGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCAAc                                                               >  7:9812/1‑136 (MQ=255)
                                                                      cTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCAACGAg                                                            >  7:70969/1‑136 (MQ=255)
                                                                         gggATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCAACGAGtt                                                          >  7:359579/1‑135 (MQ=255)
                                                                         gggATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCAACGAGt                                                           >  5:164075/1‑134 (MQ=255)
                                                                         gggATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGACTGCTCAACGAGtt                                                          >  4:378916/1‑135 (MQ=255)
                                                                                 aCAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCAACGAGTTCAAGGCtt                                                  <  6:164075/135‑1 (MQ=255)
                                                                                  cAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCAACGAGTTCAAGGCttt                                                 >  2:156662/1‑135 (MQ=255)
                                                                                   aaaaCTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCAACGAGTTCAAGGCTTTCg                                               >  8:98360/1‑136 (MQ=255)
                                                                                      aCTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCAACGAGTTCAAGGCTTTCGCa                                             <  5:142169/135‑1 (MQ=255)
                                                                                             gTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCAACGAGTTCAAGGCTTTCGCAGTCAAAgg                                     >  4:318960/1‑136 (MQ=255)
                                                                                             gTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCAACGAGTTCAAGGCTTTCGCAGTCAAAg                                      <  4:290626/135‑1 (MQ=255)
                                                                                             gTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCAACGAGTTCAAGGCTTTCGCAGTCAAAg                                      <  8:67243/135‑1 (MQ=255)
                                                                                              ttCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCAACGAGTTCAAGGCTTTCGCAGTCAAAg                                      <  1:29765/134‑1 (MQ=255)
                                                                                                            agtAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCACCGAGTTCAAGGCTTTCGCAGTCAACGGCAATGTCGTCGCCAt                      >  4:372882/1‑136 (MQ=255)
                                                                                                                 cccTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCAACGAGTTCAAGGCTTTCGCAGTCAAAGGCAATGTCGTCGACATGGcg                  >  5:207562/1‑135 (MQ=255)
                                                                                                                 cccTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCAACGAGTTCAAGGCTTTCGCAGTCAAAGGCAATGTCGTCGACATGGc                   <  2:168266/134‑1 (MQ=255)
                                                                                                                 cccTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCAACGAGTTCAAGGCTTTCGCAGTCAAAGGCAATGTCGTCGACATGGc                   <  2:119572/134‑1 (MQ=255)
                                                                                                                 cccTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCAACGAGTTCAAGGCGTTCGCAGTCAAAGGCAATGTCGTCGACATGGc                   <  6:35847/134‑1 (MQ=255)
                                                                                                                         acaACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCAACGAGTTCAAGGCTTTCGCAGTCAAAGGCAATGTCGTCGACATGGCGGTCGGTat          >  8:39044/1‑135 (MQ=255)
                                                                                                                         acaACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCAACGAGTTCAAGGCTTTCGCAGTCAAAGGCAATGTCGTCGACATGGCGGTCGGTat          >  5:261009/1‑135 (MQ=255)
                                                                                                                              gCATGAAGTGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTc                                                                  >  3:256470/1‑74 (MQ=255)
                                                                                                                              gCATGAAGTGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTc                                                                  >  3:251586/1‑74 (MQ=255)
                                                                                                                              gCATGAAGTGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTc                                                                  <  4:251586/74‑1 (MQ=255)
                                                                                                                              gCATGAAGTGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTc                                                                  <  4:256470/74‑1 (MQ=255)
                                                                                                                              gCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCCTGCTCAACGAGTTCAAGGCTTTCGCAGTCAAAGGCAATGTCGTCGACATGGCGGTCGGTatcatcat    >  8:46378/1‑136 (MQ=255)
                                                                                                                              gCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCAACGAGTTCAAGGCTTTCGCAGTCAAAGGCAATGTCGTCGACATGGCGGTCGGTatcatca     >  1:290893/1‑135 (MQ=255)
                                                                                                                                 tGAAGCGTTTAACTGGCTTACACTGCCTTAACCTTACCTTTTTTTACAAGGAAACAAACATCGGCTTGCTCAACTAGTTCAAGGCTTTCGCAGTCAAAGGCAATGTCGTCGACATGGCGGTCGGTATCATCATCg  <  1:201050/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                 
GGCCTTGGCCATGGGTGAGGTCTCCTGGAAAAATTCGCCATGATAGCTGAGCAGGACGTGTCTCGAACGCCTCGGGATGCGACAAAACTATTCGTTCTAACTGCAGTCAGTAGCCCTGAACACAACGCATGAAGCGTTTAAGTGGCTTACACTGCCTTAACCTTACCTTTTGTTACAAGGAACCAAACATGGGCATGCTCAACGAGTTCAAGGCTTTCGCAGTCAAAGGCAATGTCGTCGACATGGCGGTCGGTATCATCATCG  >  ACB122/5264295‑5264558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑