Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,270,972 C→T 6.8% intergenic (‑308/‑36) cydA ← / → entS_1 Cytochrome bd‑I ubiquinol oxidase subunit 1/Enterobactin exporter EntS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,270,9720CT 6.8% 181.2 / 3.5 60intergenic (‑308/‑36)cydA/entS_1Cytochrome bd‑I ubiquinol oxidase subunit 1/Enterobactin exporter EntS
Reads supporting (aligned to +/- strand):  ref base C (30/24);  new base T (2/2);  total (34/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-01

CATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCGGCTTCCAGA  >  ACB122/5270838‑5271098
                                                                                                                                      |                                                                                                                              
cATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTgcg                                                                                                                               <  8:93828/136‑1 (MQ=255)
  tAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGcc                                                                                                                             <  7:314754/136‑1 (MQ=255)
  tAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGcc                                                                                                                             <  7:261670/136‑1 (MQ=255)
   aGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGcc                                                                                                                             >  2:63943/1‑135 (MQ=255)
   aGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGAAGAAACGCTGAGccc                                                                                                                            >  8:208033/1‑136 (MQ=255)
      cccGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGTTTAGCAGTGGAAAAGTGCCGACCGGAGCAAACAGTAACCGCTTGTTACAAACACAAGATACGCTGCGcccc                                                                                                                           >  5:111118/1‑134 (MQ=255)
      cccGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGccccc                                                                                                                          >  8:365562/1‑135 (MQ=255)
             ggCCCGGTGGGCCGTGTGGTGGATTTCCGCGTGACCTGTTTCCTCACCCTCATTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTg                                                                                                                  >  7:11879/1‑136 (MQ=255)
             ggCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGCCCTGTTGCCGCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCCAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTg                                                                                                                  >  3:106249/1‑136 (MQ=255)
             ggCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCAGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTg                                                                                                                  >  3:62419/1‑136 (MQ=255)
                     gggCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAgg                                                                                                          <  3:200651/136‑1 (MQ=255)
                              ggtggATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCAGAGGCCCCTGGtt                                                                                                 >  3:149884/1‑136 (MQ=255)
                                     tGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGTGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGAt                                                                                           >  1:19106/1‑135 (MQ=255)
                                     tGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGTGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGAt                                                                                           >  1:26103/1‑135 (MQ=255)
                                     tGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGAt                                                                                           <  2:293573/135‑1 (MQ=255)
                                     tGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGAt                                                                                           <  5:143977/135‑1 (MQ=255)
                                        cgcgTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTcc                                                                                       >  3:290420/1‑136 (MQ=255)
                                          cgTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCg                                                                                      >  2:72267/1‑135 (MQ=255)
                                          cgTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGAGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCg                                                                                      >  1:249175/1‑135 (MQ=255)
                                             gACCTGTTGCCTCACACTCTTTTATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGAtt                                                                                   <  5:98572/135‑1 (MQ=255)
                                               ccTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTccg                                                                                >  7:104839/1‑136 (MQ=255)
                                                   ttGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTccgca                                                                              <  4:224472/134‑1 (MQ=255)
                                                    tGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAATCAGATGATACGCTGCGCCCCCCTCTACAGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTccgcacc                                                                            >  6:196421/1‑135 (MQ=255)
                                                    tGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTccgca                                                                              <  6:207494/133‑1 (MQ=255)
                                                       cTCACCCTCTTTGATTATCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACAGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCGCCTGGTTTCCGATGTCCGATTccgcagcgc                                                                          <  5:196421/134‑1 (MQ=255)
                                                          aCCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTTCATACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAgt                                                                       <  8:193994/134‑1 (MQ=255)
                                                          aCCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAgt                                                                       <  7:212026/134‑1 (MQ=255)
                                                            ccTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAgttgt                                                                    >  6:332599/1‑135 (MQ=255)
                                                                   gATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGAtt                                                                                   >  1:109951/1‑113 (MQ=255)
                                                                   gATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGAtt                                                                                   <  2:109951/113‑1 (MQ=255)
                                                                   gATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCAc                                                            >  3:157546/1‑136 (MQ=255)
                                                                   gATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCAc                                                            >  8:46867/1‑136 (MQ=255)
                                                                   gATTAGCAGTGGAAAAGAGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCAc                                                            >  7:136781/1‑136 (MQ=255)
                                                                       aGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCAt                                                         <  4:62419/135‑1 (MQ=255)
                                                                        gCAGTGGAATAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCGCCCTCTACTGCGCCGAGGCCCCTTGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATc                                                        <  3:274388/135‑1 (MQ=255)
                                                                         cAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATc                                                        <  1:358403/134‑1 (MQ=255)
                                                                            tGGAAAAGTGCCGACCTGAGCAAACAGTAGCCGCTTGTTACAAACAGATGATCCGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTcc                                                    >  1:284402/1‑135 (MQ=255)
                                                                             ggAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCAGCACCGCAGTTGTTGCGTCACCATCGTCCt                                                   >  1:76947/1‑135 (MQ=255)
                                                                              gAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCttt                                                 <  4:26131/136‑1 (MQ=255)
                                                                               aacaGTGCCGACCTGAGCACACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTCGTTGCGTCACCATCGTCCttt                                                 <  6:127206/132‑1 (MQ=255)
                                                                                        gACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATCATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTc                                       >  7:334602/1‑136 (MQ=255)
                                                                                          ccTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTctg                                     <  4:330110/136‑1 (MQ=255)
                                                                                          ccTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTct                                      <  1:384449/135‑1 (MQ=255)
                                                                                           cTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTgcgc                                                                                                               <  8:48201/61‑1 (MQ=255)
                                                                                           cTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTgcgc                                                                                                               >  7:48201/1‑61 (MQ=255)
                                                                                                 aaaCAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCtggctggc                               <  5:180933/135‑1 (MQ=255)
                                                                                                 aaaCAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGcc                              >  4:116339/1‑136 (MQ=255)
                                                                                                 aaaCAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTGTTCTGGCCTTCTGGCTGGcc                              >  3:152145/1‑136 (MQ=255)
                                                                                                           cGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCGTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCGTTTTCTGGCCTTCTGGCTTGCCCGCGTGTTc                     >  5:68153/1‑135 (MQ=255)
                                                                                                           cGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTc                     >  5:137506/1‑135 (MQ=255)
                                                                                                                gTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGc                >  6:208164/1‑135 (MQ=255)
                                                                                                                  tACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAg             >  3:216815/1‑136 (MQ=255)
                                                                                                                  tACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAg             >  3:302107/1‑136 (MQ=255)
                                                                                                                     aaaCAGATGATACGCTGTGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCg           <  2:19106/135‑1 (MQ=255)
                                                                                                                     aaaCAGATGATACGCTGTGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCg           <  2:26103/135‑1 (MQ=255)
                                                                                                                      aaCAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGcc                                                                                                        <  4:232616/41‑1 (MQ=255)
                                                                                                                      aaCAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGcc                                                                                                        >  3:232616/1‑41 (MQ=255)
                                                                                                                        cAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCGGCtt       <  7:365562/136‑1 (MQ=255)
                                                                                                                              atACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCGGCTTCCAGa  >  1:32299/1‑135 (MQ=255)
                                                                                                                              atACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCGGCTTCCAGa  >  2:241217/1‑135 (MQ=255)
                                                                                                                               tACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCGGCTTCCAGa  <  5:227212/134‑1 (MQ=255)
                                                                                                                                      |                                                                                                                              
CATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGATGATACGCTGCGCCCCCCTCTACTGCGCCGAGGCCCCTGGTTTCCGATGTCCGATTCCGCACCGCAGTTGTTGCGTCACCATCGTCCTTTTCTGGCCTTCTGGCTGGCCCGCGTGTTCACCGCCAGCGGCTTCCAGA  >  ACB122/5270838‑5271098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑