Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,284,855 T→G 18.8% F162C (TTT→TGT)  cmpR_6 → HTH‑type transcriptional activator CmpR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,284,8550TG18.8% 102.7 / 3.8 55F162C (TTT→TGT) cmpR_6HTH‑type transcriptional activator CmpR
Reads supporting (aligned to +/- strand):  ref base T (10/33);  new base G (10/2);  total (20/35)
Fisher's exact test for biased strand distribution p-value = 2.64e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAACCCTGGAGTCAGCGTGGATGTGCAAGAGCTGCCGAGTTTGCGCATCGTGCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACGCCACAGGCCCACCCCCTGGCCAGTGCGCTGGCGCCAAGCTTCGTCGACAGCCTGGCCCATGGCTATGTCGGCCTGGGGGCCGGCAGTGCACTGGGGTTGTACCT  >  ACB122/5284723‑5284987
                                                                                                                                    |                                                                                                                                    
aaaaCCCTGGAGTCAGCGTGGATGTGCAAGAGCTGCCGAGTTTGCGCATCGTGCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCg                                                                                                                                   <  8:332000/136‑1 (MQ=255)
  aaCCCTGGAGTCAGCGTGGATGTGCAAGAGCTGCCGAGTTTGCGCATCGTGCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTc                                                                                                                                    >  4:168345/1‑133 (MQ=255)
          aGTCAGCGTGGATGTGCAAGAGCTGCCGAGTTTGCGCATCGTGCAGGCAAGTACCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGAcc                                                                                                                           <  2:151838/134‑1 (MQ=255)
          aGTCAGCGTGGATGTGCAAGAGCTGCCGAGTTTGCGCAGCGTGCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATCGTGGCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGAc                                                                                                                            <  2:268768/133‑1 (MQ=255)
           gTCAGCGTGGATGTGCAAGAGCTGCCGAGTTTGCGCATCGTGCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCg                                                                                                                         <  5:90653/135‑1 (MQ=255)
               gCGTGGATGTGCAAGAGCTGCCGAGTTTGCGCATCGTGCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATGGTGTCCACAGCCGCCCCCAGCGAGCACCTGGAGACCCGGCGGTTTCGAGATGACCCgctggt                                                                                                                    <  8:311312/136‑1 (MQ=255)
               gCGTGGATGTGCAAGAGCTGCCGAGTTTGCGCATCGTGCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCgctg                                                                                                                      <  1:61047/134‑1 (MQ=255)
                cGTGGATGTGCATGAGCTGCCGAGTTTGCGCATCGTGCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGAGCCGGCCGTTTCGAGATGACCCgctggt                                                                                                                    <  2:43970/135‑1 (MQ=255)
                    gATGTGCAAGAGCTGCCGAGGTTGCGCATCGTGCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCgctggtgctgg                                                                                                               <  8:197229/136‑1 (MQ=255)
                         gCAAGAGCTGCCGAGTTTGCGCATCGTGCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGAGGACCCGCTGGTGCTGGTGACg                                                                                                          <  8:192689/136‑1 (MQ=255)
                          cAAGAGCTGCCGAGTTTGCGCATCGTGCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACg                                                                                                          <  1:139912/135‑1 (MQ=255)
                          cAAGAGCTGCCGAGTTTGCGCATCGTGCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACg                                                                                                          <  2:74044/135‑1 (MQ=255)
                           aaGAGCTGCCGAGTTTGCGCATCGTGCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACg                                                                                                          <  2:48426/134‑1 (MQ=255)
                             gagCTGCCGAGTTTGCGCATCGTGCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCgaga                                                                                                                                <  8:27744/110‑1 (MQ=255)
                             gagCTGCCGAGTTTGCGCATCGTGCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCgaga                                                                                                                                >  7:27744/1‑110 (MQ=255)
                                 tGCCGAGTTTGCGCATCGTGCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACGCCACAgg                                                                                                   <  1:302969/135‑1 (MQ=255)
                                 tGCCGAGTTTGCGCATCGTGCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTGCGAGAAGACCCGCCGGTGCTGGTGGCGCcacacg                                                                                                   >  6:95032/1‑133 (MQ=255)
                                   ccGAGTTTGCGCATCGTGCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACGCCCCCGGccc                                                                                                >  8:26564/1‑136 (MQ=255)
                                     gAGTTTGCGCATCGTGCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTGTCGAGATGACCCGCTGGTGCTGGGGACGCCCCAGGcccc                                                                                               >  2:293200/1‑134 (MQ=255)
                                      aGTTTGCGCATCGTGCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACGCCACAGGCCCAc                                                                                              <  2:82986/135‑1 (MQ=255)
                                             gcATCGTGCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTGTCGAGATGACCCGCTGGTGCTGGTGACGCCACAGGCCCCCCCCCTGGc                                                                                      >  3:184956/1‑136 (MQ=255)
                                                    gCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACGCCACAGGCCCACCCCCTGGCCAGTgc                                                                                <  5:347990/135‑1 (MQ=255)
                                                    gCAGGCAATTACCCAAGGCATGGCCGACCTGGGAATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTGGAGATGACCCGCTGGTGCTGGTGACGCCACAGGCCCACCCCCGGGcccgggc                                                                                >  6:68414/1‑130 (MQ=255)
                                                              aCCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGCCCCGGCCGTGTGGAGCTGACCCGCTGGGGCTGGTGACGCCCCCCGCCCCCCCCCAGGCCCGTGCGCTGGCGccca                                                                     >  8:387487/1‑134 (MQ=255)
                                                               cccAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACGCCACAGGCCCACCCCCTGGCCAGTGCGCTGGCGCCaa                                                                     <  2:173382/135‑1 (MQ=255)
                                                                ccAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTGTCGAGATGACCCGCGGGTGCTGGTGCCGCCCCACGCCCCCCCCCTGGCCAGTGCGCTGGCGCCaa                                                                     >  6:294203/1‑134 (MQ=255)
                                                                     gCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACGCCACAGGCCCACCCCCTGGCCAGTGCGCTGGCGCCAAGCTtcgt                                                              <  8:136707/136‑1 (MQ=255)
                                                                     gCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTGTCGAGCTGACCCCCTGGTGCAGGTGCCGCCAAAGGCCCCCCCCCTGGCCAGTGCGCAGGCGCCCAGCTtcgt                                                              >  7:306919/1‑136 (MQ=255)
                                                                       aTGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGCTGACCCGCTGGTGCTGGTGACGCCCCACGCCCCCCCCCAGGCCACTGCGCTGGCGCCAAGCTtcgtc                                                             >  6:326573/1‑135 (MQ=255)
                                                                       aTGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTGTGGAGATGACCCCCGGGTGCTGGTGACGCCCCCCGCCCCCCCCCGGGCCAGTGCGCTGGCGCCCAGCTtcgtcg                                                            >  3:225926/1‑136 (MQ=255)
                                                                       aTGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTGTCGAGATGACCCGCGGGTGCTGGTGCCGCCACAGGCCCCCCCCCAGGCCACTGCGCGGGCGCCAAGCAtagtc                                                             >  6:16424/1‑135 (MQ=255)
                                                                          gCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCAGCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACGCCACAGGCCCACCCCCTGGCCAGTGCGCTGGCGCCAAGCTTCGTCGACa                                                         <  7:350130/136‑1 (MQ=255)
                                                                          gCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACGCCACAGGCCCACCCCCTGGCCAGTGCGCTGGCGCCAAGCTTCGTCGACa                                                         <  8:54142/136‑1 (MQ=255)
                                                                          gCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACGCCACAGGCCCACCCCCTGGCCAGTGCGCTGGCGCCAAGCTTCGTCGACa                                                         <  3:188517/136‑1 (MQ=255)
                                                                          gCCGACCTGGGCAGCGTGTCCCCAGCCGCCCCCAGCGAGCCCCTGCAGACCCGGCCGTGTCGAGATGACCCGCTGGTGCTGGTGACGCCACAGGCCCACCCCCTGGCCAGTGCGCTGGCGCCAAGCTTCGTCGACa                                                         <  8:48184/136‑1 (MQ=255)
                                                                           ccGACCTGGGCAGCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACGCCACAGGCCCACCCCCTGGCCAGTGCGCGGGCGCCAAGCTTCGTCGACa                                                         <  1:180466/135‑1 (MQ=255)
                                                                            cGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTGTCGAGATGACCCGCGGGTGCTGGTGACGCCACACGCCCACCCCCCGGCCCGTGCGCTGGCGCCCAGCTTCGTCGCCAg                                                        >  1:10987/1‑135 (MQ=255)
                                                                               ccTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTTGTGCTGGTGACGCGACAGGCCCACCCCCTGGCCAGTGCGCTGGCGCCAAGCTTCGTCGACAGCCTg                                                    <  3:195882/136‑1 (MQ=255)
                                                                               ccTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACGCCACAGGCCCACCCCCTGGCCAGTGCGCTGGCTCCAAGCTTCGTCGACAGCCTg                                                    <  8:198345/136‑1 (MQ=255)
                                                                                cTGGGCATCGTGGCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACGCCACAGGCCCACCCCCTGGCCAGTGCGCTGGCGCCAAGCTTCGTCGACAGCCTg                                                    <  2:178733/135‑1 (MQ=255)
                                                                                 tGGGCATCGTGTCCACAGCCGCCCCCAGCGCGCACCTGCAGCCCCGGCCGTGTCGAGATGACCCGCTGGTGCTGGTGACGCCACAGGCCCCCCCCCGGGCCCCTGCGCGGGCGCCCAGCGTCGGGGACAGCCGGGc                                                  >  7:203062/1‑136 (MQ=255)
                                                                                 tGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCGGGTGGCGCCCCAGGCCCACCCCCAGGCCCGGGCGCTGGCGCCCCGCATCGTCGCCAGCCggg                                                   >  1:193531/1‑135 (MQ=255)
                                                                                  tggCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTGTCGAGATGACCCGCTGGTGCTGGTGCCGCCACACGCCCACCCCCAGGCCAGTGCGCTGGCGCCAAGCTTCGTCGACAGCCGGGc                                                  >  2:14949/2‑135 (MQ=255)
                                                                                    gcctCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCAGTTTCGAGATGACCCGCGGGTGCTGGTGACGCCACAGGCACACCCCCTGGCCAGGGCGCGGGCGCCAAGCTTCGGCGACAGCCTGGCCCa                                               <  3:110994/133‑1 (MQ=255)
                                                                                          tgtCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGGGACGCCACAGGCCCACCCCCTGGCCAGTGCGCTGGCGCCAAGCTTCGTCGACAGCCTGGCCCATGGCTa                                         <  7:100724/136‑1 (MQ=255)
                                                                                             ccACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACGCCCCACGCCCCCCCCCAGGCCAGTGCGCTGGCGCCAAGCGTCGTCGACAGCCTGGCCCATGGGGATGt                                      >  4:151202/1‑136 (MQ=255)
                                                                                                caGCCACCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACGCCACAgg                                                                                                   >  2:46601/1‑72 (MQ=255)
                                                                                                caGCCACCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACGCCACAgg                                                                                                   <  1:46601/72‑1 (MQ=255)
                                                                                                       ccccCGCGCGCACCGGCCGCCCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACGCCACAGGCCCACCCCCTGGCCAGTGCGCTGGCGCCAAGCTTCGTCGACAGCCTGGCCCATGGCTATGTCGGCCTggg                             <  1:191538/135‑1 (MQ=255)
                                                                                                       ccccAGCGAGCACCTGCAGACCCGGCCGGTTCGAGATGACCCGCTGGTGCTGGTGACGCCACAGGCCCACCCCCTGGCCAGTGCGCTGGCGCCAAGCTTCGTCGACAGCCTGGCCCATGGCTATGTCGGCCTggg                             <  1:161549/135‑1 (MQ=255)
                                                                                                            gCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACGCCACAGGCCCACCCCCTGGCCAGTGCGCTGGCGCCAAGCTTCGTCGACAGCCTGGCCCATGGCTATGTCGGCCTGGGGGCCgg                       <  4:295845/136‑1 (MQ=255)
                                                                                                              gAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACGCCACAGGCCCACCCCCTGGCCAGTGCGCTGGCGCCAAGCTTCGTCGACAGCCTGGCCCATGGCTATGTCGGCCTGGGGGCCGGCa                     <  7:94318/136‑1 (MQ=255)
                                                                                                                 cACCTGCAGACCAGGCAGTGTCGAGATGACCCGCTGGTGCTGGTGACGCAACAGGCCCACCCCCTGGCCAGTGCGCGGGCGCCAAGATTCGTCTACATCCTTGCCCATGGCTATGTCGGCCTGGGGGCCGGCAGTg                  <  8:276954/136‑1 (MQ=255)
                                                                                                                       cAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACGCCACAGGCCCACCCCCTGGCCAGTGCGCTGGCGCCAAGCTTCGTCGACAGCCTGGCCCATGGCTATGTCGGCCTGGGGGCCGGCAGTGCACTg             <  4:225926/135‑1 (MQ=255)
                                                                                                                                 cGTTTCGAGATGACCCGCTGGTGCTGGTGACGCCACACGCCCACCCCCAGGCCAGTTCGCTGGCGCCAAGCATCGTCGACACCCTGGCCCATGGCGATTTCGGCCTGGGGGCCGGGAGTGCACTGGGGTTGTACCt  >  8:100629/1‑136 (MQ=255)
                                                                                                                                    |                                                                                                                                    
AAAACCCTGGAGTCAGCGTGGATGTGCAAGAGCTGCCGAGTTTGCGCATCGTGCAGGCAATTACCCAAGGCATGGCCGACCTGGGCATCGTGTCCACAGCCGCCCCCAGCGAGCACCTGCAGACCCGGCCGTTTCGAGATGACCCGCTGGTGCTGGTGACGCCACAGGCCCACCCCCTGGCCAGTGCGCTGGCGCCAAGCTTCGTCGACAGCCTGGCCCATGGCTATGTCGGCCTGGGGGCCGGCAGTGCACTGGGGTTGTACCT  >  ACB122/5284723‑5284987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑