Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,613,064 C→T 6.3% P13P (CCG→CCA)  mshA_6 ← D‑inositol‑3‑phosphate glycosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,613,0640CT 6.3% 247.8 / 4.8 79P13P (CCG→CCA) mshA_6D‑inositol‑3‑phosphate glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (38/36);  new base T (2/3);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

TGAGGGCATATGCCATTCCCGGGTCGAACCGAGCCCACAGCAGAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCATTGAACAAAAATGGTGATTCCCATGCCGATCAAGGCCCCCGCAACCAGCGTCAAT  >  ACB122/5612929‑5613197
                                                                                                                                       |                                                                                                                                     
tGAGGGCATATGCCATTCCCGGGTCGAACCGAGCCCACAGCAGAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTAc                                                                                                                                       >  7:137923/1‑136 (MQ=255)
  aGGGCATATGCCATTCCCGGGTCGAACCGAGCCCACAGCAGAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACg                                                                                                                                      <  4:17615/135‑1 (MQ=255)
       atatGCCATTCCCGGGTCGAACCGAGCCCACAGCAGAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTc                                                                                                                                <  7:233506/136‑1 (MQ=255)
       atatGCCATTCCCGGGTCGAACCGAGCCCACAGCAGAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTc                                                                                                                                <  7:377386/136‑1 (MQ=255)
          tGCCATTCCCGGGTCGAACCGCGCCCACAGCAGAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCAc                                                                                                                             >  4:333044/1‑136 (MQ=255)
            ccATTCCCGGGTCGAACCGAGCCCACAGCAGAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCcccc                                                                                                                            >  5:6141/1‑135 (MQ=255)
            ccATTCCCGGGTCGAACCGACCCCACAGCAGAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCAGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCAccc                                                                                                                           >  4:205123/1‑136 (MQ=255)
                 cccGGGTCGAACCGAGCCCACAGCAGAGCCCGCTCACCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCca                                                                                                                      >  7:211677/1‑136 (MQ=255)
                  ccGGGTCGAACCGAGCCCACAGCAGAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCCCCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCca                                                                                                                      >  7:115575/1‑135 (MQ=255)
                  ccGGGTCGAACCGAGCCCACAGCAGAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTATGGCGTTCCACCCGccc                                                                                                                       >  1:171411/1‑134 (MQ=255)
                  ccGGGTCGAACCGAGCCCACAGCAGAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTATGGCGTTCCACCCGccc                                                                                                                       >  1:176166/1‑134 (MQ=255)
                  ccGGGTCGAACCGAGCCCACAGCAGAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCca                                                                                                                      <  7:179581/135‑1 (MQ=255)
                  ccGGGTCGAACCGAGCCCACAGCAGAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCca                                                                                                                      >  7:98998/1‑135 (MQ=255)
                  ccGGGTCGAACCGAGCCCACAGCAGAGCCCGCTCCCCAGCAGCAGCCACTTACTGCTCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTAGTGGATGGACATGTACGGCGTTCCACCCGCCca                                                                                                                      >  8:17480/1‑135 (MQ=255)
                   cGGGTCGAACCGAGCCCACAGCAGAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTATGGCGTTCCACCCGCCca                                                                                                                      <  5:104348/134‑1 (MQ=255)
                   cGGGTCGAACCGAGCCCACAGCAGAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAACACCTGCCGGTGCCCCTTGTGGATGGCCATGTCCGGCGTTCCACCCGcccc                                                                                                                      >  2:177338/1‑133 (MQ=255)
                          aaCCGAGCCCACAGCAGAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCatat                                                                                                              <  5:139719/135‑1 (MQ=255)
                           aCCGAGCCCACAGCAGAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTg                                                                                                            <  8:137067/136‑1 (MQ=255)
                            ccGAGCCCACAGCAGAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTATGGCGTTCCACCCGCCCACATCATATTg                                                                                                            <  2:176166/135‑1 (MQ=255)
                            ccGAGCCCACAGCAGAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTATGGCGTTCCACCCGCCCACATCATATTg                                                                                                            <  2:171411/135‑1 (MQ=255)
                            ccGAGCCCACAGCAGAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGa                                                                                                           <  8:137923/136‑1 (MQ=255)
                                  ccACAGCAGAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATg                                                                                                      >  6:75575/1‑135 (MQ=255)
                                   cacaGCAGAGCGCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGGATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGtt                                                                                                    <  8:85901/136‑1 (MQ=255)
                                        cagAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTg                                                                                                            >  5:29894/1‑123 (MQ=255)
                                        cagAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTg                                                                                                            <  6:29894/123‑1 (MQ=255)
                                        cagAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATgg                                                                                               >  4:151385/1‑136 (MQ=255)
                                        cagAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATgg                                                                                               <  7:55784/136‑1 (MQ=255)
                                        cagAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCAGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTg                                                                                                            <  5:325959/123‑1 (MQ=255)
                                        cagAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCAGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTg                                                                                                            >  6:325959/1‑123 (MQ=255)
                                             cccGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCct                                                                                          <  7:340463/136‑1 (MQ=255)
                                              ccGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCAccc                                                                                                                           >  8:193191/1‑102 (MQ=255)
                                              ccGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCAccc                                                                                                                           <  7:193191/102‑1 (MQ=255)
                                              ccGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCct                                                                                          >  5:173080/1‑135 (MQ=255)
                                              ccGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGGTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCct                                                                                          <  5:56548/135‑1 (MQ=255)
                                                   ccccAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTccc                                                                                     >  6:353883/1‑135 (MQ=255)
                                                   ccccAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCATGTGGATGGACATGTACGGCGTTCCACCCCCCCACATAATATTGCCGATGTTCATGGGCCCTCTccc                                                                                     >  6:250123/1‑135 (MQ=255)
                                                     ccAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCTCCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCGCTCTccc                                                                                     <  5:250123/133‑1 (MQ=255)
                                                     ccAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTccc                                                                                     <  6:173080/133‑1 (MQ=255)
                                                     ccAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTccc                                                                                     <  2:365846/133‑1 (MQ=255)
                                                        gcagcagcCAGTTACTGCTCCGGGCATCGGCACCCTCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGAAATGTACGGCGTTCCACCCGCCAACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCa                                                                                <  2:229448/135‑1 (MQ=255)
                                                                 aGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGAACATTGTGGATGGACATGTACGGCGTTCCACCAGCCCACATAATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTgg                                                                      >  3:386890/1‑136 (MQ=255)
                                                                      cTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACa                                                                  >  6:363063/1‑135 (MQ=255)
                                                                            cGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCAcc                                                                                                                            <  1:87815/71‑1 (MQ=255)
                                                                            cGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCAcc                                                                                                                            >  2:87815/1‑71 (MQ=255)
                                                                            cGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGCCt                                                            >  1:345117/1‑135 (MQ=255)
                                                                            cGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACt                                                            <  8:98998/135‑1 (MQ=255)
                                                                            cGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACt                                                            <  3:333044/135‑1 (MQ=255)
                                                                              ggCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACtt                                                           >  1:186253/1‑134 (MQ=255)
                                                                              ggCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTc                                                          >  1:37031/1‑135 (MQ=255)
                                                                              ggCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATAATGACGATGTTCATGGGCCCTCTCCCGATCAAGTCAGGTGGGACAGCGACTTc                                                          <  1:177338/135‑1 (MQ=255)
                                                                                cATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCAtt                                                       <  7:17480/136‑1 (MQ=255)
                                                                                      cACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTc                                                                                 <  7:92653/104‑1 (MQ=255)
                                                                                      cACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTc                                                                                 >  8:92653/1‑104 (MQ=255)
                                                                                      cACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCATTGAACa                                                  >  8:3034/1‑135 (MQ=255)
                                                                                          cgcgTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCATTGAACAAAAAt                                             <  8:360720/136‑1 (MQ=255)
                                                                                           gcgTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCATTGAACAAAAAt                                             <  6:110803/135‑1 (MQ=255)
                                                                                           gcgTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCATTGAACAAAAAt                                             <  2:238486/135‑1 (MQ=255)
                                                                                           gcgTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGACCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCATTGAACAAAAAt                                             <  5:241041/135‑1 (MQ=255)
                                                                                                   cAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGccc                                                                                                                       <  8:83748/53‑1 (MQ=255)
                                                                                                   cAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGccc                                                                                                                       >  7:83748/1‑53 (MQ=255)
                                                                                                    aaGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCATTGAACAAAAATGGTGATTccc                                   <  7:3034/136‑1 (MQ=255)
                                                                                                     aGACCAGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCATTGAACAAAAATGGTGATTccc                                   <  5:240771/135‑1 (MQ=255)
                                                                                                         cTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCTCCCGCCAACATCATATTGACGATGTTCATGGGACCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCATTGAACAAAAATGGTGATTCCCATGc                               >  2:21340/1‑135 (MQ=255)
                                                                                                         cTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCATTGAACAAAAATGGTGATTCCCATGcc                              <  3:205123/136‑1 (MQ=255)
                                                                                                              ggTGCACCTTGTGGATTGACATGTACGGAGTTCCACCAGCCCACATCATAATCACGATGTTCATTGGCCCTCTCCCGCTCAAGTCAGGTGGGCCAGCGCCTTCAATCACAAAAAATCGAGCTTCCCTTGCAGATc                          >  6:239872/1‑135 (MQ=255)
                                                                                                                   aCCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCATTGAACAAAAATGGTGATTCCCATGCCGATCAAGGc                     >  5:222088/1‑135 (MQ=255)
                                                                                                                    ccTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGACAGGAGGGACAGCCACTACATTGAACAAAAAATGAGATACCCATGCCGATCAAGGcc                    >  5:211537/1‑135 (MQ=255)
                                                                                                                          ggatggaCATGTACGGCGTTCCACCCGCCGACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCATTGAACAAAAATGGTGATTCCCATGCCGATCAAGGCCCCCGc               <  6:209072/134‑1 (MQ=255)
                                                                                                                          ggatggaCATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCATTGAACAAAAATGGTGATTCCCATGCCGATCAAGGCCCCCGCaa             >  8:51487/1‑136 (MQ=255)
                                                                                                                          ggatggaCATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCATTGAACAAAAATGGTGATTCCCATGCCGATCAAGGCCCCCGCa              >  1:121511/1‑135 (MQ=255)
                                                                                                                          ggatggaCATGTACGGCGTTCCACCCGCCCACATCATATAGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCATTGAACAAAAATGCTGATTCCCATGCCGATCAAGGCCCCCGCa              >  5:164147/1‑135 (MQ=255)
                                                                                                                           gatggaCATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCATTGAACAAAAATGGTGATTCCCATGCCGATCAAGGCCCCCGCaa             >  5:66698/1‑135 (MQ=255)
                                                                                                                                aCATGTACGGCGTTCCACCCGCCCACATCAGATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGCCTTCATTGAACAAAAATGGTGATTCCCATGCCGATCAAGGCCCCCGCAACCAGc        >  2:307382/1‑135 (MQ=255)
                                                                                                                                  aTGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCAtt                                                       <  3:315563/86‑1 (MQ=255)
                                                                                                                                  aTGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCAtt                                                       >  4:315563/1‑86 (MQ=255)
                                                                                                                                  aTGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCATTGAACAAAAATGGTGATTCCCATGCCGATCAAGGCCCCCGCACCCAGCg       >  6:295770/1‑134 (MQ=255)
                                                                                                                                  aTGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCATTGAACAAAAATGGTGATTCCCATGCCGATCAAGGCCCCCGCAACCAGCGt      >  7:356279/1‑135 (MQ=255)
                                                                                                                                      aCGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCATTGAACAAAAATGGTGATTCCCATGCCGATCAAGGCCCCCGCAACCAGCGTCaa   <  6:334919/134‑1 (MQ=255)
                                                                                                                                      aCGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCATTGAACAAAAATGGTGATTCCCATGCCGATCAAGGCCCCCGCAACCAGCGTCAAt  >  5:172948/1‑135 (MQ=255)
                                                                                                                                      aCGGCGGTCCACCCGCCCACATCATATTGACGATGTTCATGGGCACTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCATTGAACAAAAATGGTGATTCCCATGCCGATCAAGGCCCCCGCAACCAGCGTCa    <  6:6141/133‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
TGAGGGCATATGCCATTCCCGGGTCGAACCGAGCCCACAGCAGAGCCCGCTCCCCAGCAGCAGCCAGTTACTGATCCGGGCATCGGCACCCGCGTTCGACAAGACCTGCCGGTGCACCTTGTGGATGGACATGTACGGCGTTCCACCCGCCCACATCATATTGACGATGTTCATGGGCCCTCTCCCGCTCAAGTCAGGTGGGACAGCGACTTCATTGAACAAAAATGGTGATTCCCATGCCGATCAAGGCCCCCGCAACCAGCGTCAAT  >  ACB122/5612929‑5613197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑