Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	ACB122	4,257,352	+GCGGCCGC	intergenic (‑281/+7)	CJ019_03991 ← / ← CJ019_03994	putative ABC transporter ATP‑binding protein/hypothetical protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	ACB122	= 4257351	0 (0.000)	48 (0.860) +CGCGGCCG	32/234	0.4	100%	intergenic (‑280/+8)	CJ019_03991/CJ019_03994	putative ABC transporter ATP‑binding protein/hypothetical protein
	?	ACB122	4257352 =	0 (0.000)					intergenic (‑281/+7)	CJ019_03991/CJ019_03994	putative ABC transporter ATP‑binding protein/hypothetical protein

CGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB122/4257218‑4257351 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGCGGGCACCCTGGCGGTT  >  ACB122/4257352‑4257484                                                                                                                                       ||||||||                                                                                                                                      CGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCC                                                                                                                                              <  7:58885/135‑1            TGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTCCCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCC                                                                                                                                   >  1:155849/1‑135              GATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGT                                                                                                                                 <  8:164749/135‑1                 CCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCcgctt                                                                                                                              >  2:341584/1‑130                 CCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCT                                                                                                                               >  5:83663/1‑134                   ACTAAAAACCGATAATCGCACAGAATCCGCACCAACCCAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGCATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTAC                                                                                                                            <  4:238530/135‑1                    CTAAAACCCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCACCCCGTCTTACG                                                                                                                           <  7:46798/135‑1                       AAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCCTCTTACGA                                                                                                                          >  2:15154/1‑133                        AAACCGAAACTCCCACAGAATCCGCACCTACCGAACAAAATGCATTCACGTACCCCGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGA                                                                                                                       <  7:15762/135‑1                             GATACTCGCACCTAATCCGCACCAACCGAACAAAATGCATTCACCAACCCCGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAACCCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCA                                                                                                                  <  3:288060/135‑1                                        AGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGGCTTACGACGAGGGCAGGATGACTTTC                                                                                                       >  8:352322/1‑135                                                   CTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACATTCATGCCCGCGTC                                                                                            >  5:90308/1‑135                                                   CTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGACTTACGCCGAGGGCAGGATGACTTTCATGCCCGCGTC                                                                                            >  1:93206/1‑135                                                       CGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCC                                                                                        >  6:272148/1‑135                                                       CGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCCGCGTTCGACGAGGGCCGGATGCATTTAAAGCCCGCGTCTGCC                                                                                        >  1:200080/1‑135                                                       CGAACAAAATGCATTGACCTACCACCCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTG                                                                                          <  2:259589/133‑1                                                                   ATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAACCCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGCCGAGGGCAGGATGACTTTAATGCCCGCCTCTGCCGCAGCCCCCCC                                                                             >  1:94148/1‑134                                                                   ATTGACCAACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCC                                                                             <  2:334966/134‑1                                                                      GACCTACCACGCCGACCGCAGCCTTAATGGAGCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCGTACGACGAGGGCAGGATGACTTTAATGACCGCGTCTGCCGCCGCCCCCCCAAAa                                                                         >  1:288426/1‑134                                                                       ACCAACCACGCCGACCGCAGCATTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGG                                                                        <  5:11061/135‑1                                                                        CCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGG                                                                        <  7:338360/134‑1                                                                            CCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAGGGTTTCG                                                                   <  7:122446/135‑1                                                                             CACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGGCGTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGGTTCGG                                                                  >  7:380065/1‑135                                                                               CGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCA                                                                <  1:59520/135‑1                                                                               CGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGC                                                                 >  2:135975/1‑134                                                                               CGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGGCTTACGACGAGGGCAGGATGACTTTAATGCCCGCGTCTGCCGCCGCCCCGCCAAAGGGTTAGGC                                                                 >  1:244639/1‑134                                                                                    ACCGCAGCCTTAATGGATCCATTAAATAAAAACAAACCCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCG                                                           <  4:79812/135‑1                                                                                    ACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGC                                                            >  2:185914/1‑134                                                                                    ACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGC                                                            >  8:213694/1‑134                                                                                      cgtcGCCTTAATGGATCATTTAAAAAAAAACAAACCCCGGAGCGCCTCGCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCA                                                         <  4:9078/131‑1                                                                                      ccaAGCCTTAATGCAAACATTAAAAAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCA                                                         <  1:341584/132‑1                                                                                         AGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGG                                                      >  5:308184/1‑135                                                                                              TAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCA                                                 >  4:82944/1‑135                                                                                                 TGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTG                                              >  2:6102/1‑135                                                                                                     TCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGT                                          <  1:135975/135‑1                                                                                                        ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGCCGAGGGCAGGATGCCTTTCATTCCAGCGTCTGCCCCCCCCCCGCCAAACGTTTCGGCATAGCGCCAGGTTGGATTGGCGTGCT                                       >  5:206522/1‑135                                                                                                        ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGCCGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCCGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCg                                       >  1:63195/1‑134                                                                                                        ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCT                                       >  7:217694/1‑135                                                                                                        ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCT                                       >  7:58202/1‑135                                                                                                        ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCCTCTTACGACGAGGGCAGGATGACTATCATGCCCGCGTCTGCCGCAGCCCCCCCAAAGGTTTCGGCATATCGCAAGGTGGCATTGGCGTGCT                                       >  3:101624/1‑135                                                                                                        ATTAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCCTCGTACGCCCAGGGCAGGATGACTTTAATGCCCCCGTCTGCCGCCCCCCCGCCAAACGGTTCGGCAAAGCGCAAGGTGGCATTGGCGTGCT                                       >  5:344573/1‑135                                                                                                         taAAATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCACGTCTTACGAGCATGGCAGGATGACTTTCATGCCCGCGTCTGCCGCCGCCCCGCCAAAGCTTTCGCCATAGCGCAAGGTGGCGATTGCGTGCTC                                      >  7:231197/3‑135                                                                                                            AATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCG                                                                               <  5:302202/91‑1                                                                                                            AATAAAAACAAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCG                                                                               >  6:302202/1‑91                                                                                                                     AAAACCCGGAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGTGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGC                          <  1:316751/135‑1                                                                                                                             GAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGC                  <  5:272148/135‑1                                                                                                                             GAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGC                  <  1:7746/135‑1                                                                                                                             GAGAGCCTCCCGCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGC                  <  8:342288/135‑1                                                                                                                             GAGAGCCTCCCGCGGCCGCCCCGTCGTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGC                  <  8:217694/135‑1                                                                                                                                        GCGGCCGCCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGCGGGCACCCTGG       <  4:238922/135‑1                                                                                                                                           GCCGCCCCGTCTTACGCCGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCGTCGGCGCGGGCCCCCTGGCGG    >  2:16628/1‑135                                                                                                                                             CGCCACGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGCGGGCACCCTGGCGGTT  <  1:83471/135‑1                                                                                                                                       ||||||||                                                                                                                                      CGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB122/4257218‑4257351 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCCGTCTTACGACGAGGGCAGGATGACTTTCATGCCCGCGTCTGCCGCAGCCCCGCCAAAGGTTTCGGCATAGCGCAAGGTGGCATTGGCGTGCTCGCGCATGATCGCTTCGGCGCGGGCACCCTGGCGGTT  >  ACB122/4257352‑4257484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.