| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 790,007 | 0 | T | G | 53.8% | 1.5 / 15.4 | 26 | intergenic (+159/‑31) | CJ019_00751/CJ019_00752 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (7/5); new base G (14/0); total (21/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.20e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.38e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCACAGGTACAGCATCGGCTTGCTGTGGGAGTGTGGCCTTGTGTCGCGATCAGGCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTAGTCACCTGATGAGTCC‑TGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTT > ACB122/789895‑790140 | ccACAGGTACAGCATCGGCTTGCTGTGGGAGTGTGGCCTTGTGTCGCGATCAGGCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTAGTGACCTGATGCGTcc > 2:71439/1‑135 (MQ=255) ggTACAGCATCGGCTTGCTGTGGGAGTGTGGCCTTGTGTCGCGATCAGGCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTAGTCACCTGATGAGTCC‑TGAAc > 2:235130/1‑135 (MQ=255) aTCGGCTTGCTGTGGGAGTGTGGCCTTGTGTCGCGATCAGGCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCGTTTTTAGTCACCTGATGAGTAC‑TGAACGTCaatg > 3:129307/1‑131 (MQ=255) cGGCTTGCTGTGGGAGTGTGGCCTTGTGTCGCGATCAGGCTGCGGAGCAGCACCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCGTTTTTAGTAAACTGATGAGTCC‑TGCACTTGATTGAAc > 7:318974/1‑135 (MQ=255) gCTTGCTGTGGGAGTGTGGCCTTGTGTCGCGATCAGGCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTAGTCACCTGATGAGTCC‑TGATCTTCATGGGACgg > 6:107879/1‑134 (MQ=255) cTTGCTGTGGGAGTGTGGCCTTGTGTCGCGATCAGGCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTAGTGCCCTGATGAGGCC‑TGCACTTGATGGacccga > 2:46334/1‑130 (MQ=255) ccTTGTGTCGCGATCAGGCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTTGTGACCAGATGATTGC‑TGAACATCATTGAAAGTATACTCCAAAACCCGATGt > 8:346294/1‑135 (MQ=255) ccTTGTGTCGCGATCAGGCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCGTTTTTTTTCACCCGATGAGTCC‑TGCACGTCATGGAACGGATACTAGAAAACGCGAttt > 7:265512/1‑133 (MQ=255) ttGTGTCGCGATCAGGCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTATTCACCTGATGTGTCC‑GGAACTTGATTGAACGTTTACTCGAAAACGCGATGttt > 8:208251/1‑133 (MQ=255) ttGTGTCGCGATCAGGCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCGTTTTTAGTCACCGGATGAGTCC‑GGAACATCCAGGCACCTATCCGCCAAAACCCCCTGTAt > 8:213767/1‑135 (MQ=255) tgtCGCGATCAGGCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCGTTTTTTGTCAACGGATGAGTCG‑TGAAAGTCATGGAACGTATCCTAGAAAACCCGATGTATGcc > 4:138323/1‑135 (MQ=255) tCGCGAACAGCCTGCGGAACAGCCACCGGGAGATTGCTGTAACAGGTGGCTCTCTATACTCGCCGTCGCGTTTTTATACACCAAAACAATCA‑TGAACAACATGGAAACTATCCACCAAAACACGATGTaaaccac > 4:91757/1‑129 (MQ=255) cgcgATCAGGCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCGTTTTTTTTGACCAGATTAGTCCAGGAAGTCAATGGAA‑GTTTACACGAAAAAGCCATGTATggccgg > 8:346371/1‑130 (MQ=255) cgttctggCTGGGGAGCCGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTAGTCACCTGATGAGTCC‑TGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTcgcg < 6:54637/129‑1 (MQ=255) tccggCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCGGTTTTAGTGCCCGGTTGAGTCC‑TGGACTTCATGGAACGTATACTCGAAAACCCGATGTATTCCTACCgctg > 8:102111/4‑135 (MQ=255) tCAGGCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCGTTTTTATTCACCGGATGGGTCC‑GGAACTTCATGGAACGTATCCTCGAAAACGCGATGTTTGCCGCGCgctg > 2:332203/1‑135 (MQ=255) gcagcCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTAGTCACCTGATGAGTCC‑TGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCAcc < 1:235130/135‑1 (MQ=255) gcagcCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTAGTCACCTGATGAGTCC‑TGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCAcc < 7:223318/135‑1 (MQ=255) gCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTAGTCACCTGATGAGTCC‑TGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCg < 2:239989/135‑1 (MQ=255) gCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTAGTCACCAGATGAGTCC‑GGAAATTCATGGAACGTATCCTAGAAAACGCGATGTATGCCTCCAGCTGGCTGCTCGCACCGATCTACTTCGGCgtgt > 8:122883/1‑135 (MQ=255) gCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCGTTTTTTGTAAACAGATGAGTCC‑TGAACGTAATGGAAAGTATCCTAGAAAACGCGAGTTATGCCTCCCGCTGGCTGCGCGCCCCCCTCTACTTGGGCgtgt > 6:205566/1‑135 (MQ=255) gCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCGTTTTTAGGCCCCTGATGAGTCC‑TGAACTTCATGGCACGGATACTCGAAAACGCGATGTATGCCTCGCGCTGGCGGCTCGCCCCCATCGACTTAGGCTTGt > 1:261022/1‑135 (MQ=255) ggTGGCTGGCTATACTCGCCGTCGCGTTTTTATTCACCTGATGAGTCC‑TGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGcc > 6:352899/1‑135 (MQ=255) ctggctATACTCGCCGTCGCGTTTTTTGTCCACCGGTGAGTCC‑TGCACATGATGGTACCTATCCGGGCAAACCCGGTTGATGCCGCGCGCGGGCTGCTGGCACCGGTATACATCGGCGTGTCCCACGGGcagctg > 2:87574/1‑135 (MQ=255) gctATACTCGCCGTCGCGTTTTTAGTCACCAGATGAGTCC‑TGGACTTCATGGGACGGTTGCACGAAAACGCGATGTATGCCTCGCGCGGGCTGCGCGCACCGCTATACTTCGGCGTGTCCCAcggccggcgtgcc > 4:125699/1‑130 (MQ=255) cTTTTTTAGTCACCTGATGAGGCC‑TGAACTTCATGGAACGTCTCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAAtttt < 7:334051/135‑1 (MQ=255) | CCACAGGTACAGCATCGGCTTGCTGTGGGAGTGTGGCCTTGTGTCGCGATCAGGCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTAGTCACCTGATGAGTCC‑TGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTT > ACB122/789895‑790140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |