Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,938,2390AC46.7% 6.8 / 10.8 30K537N (AAA→AAC) pqiBIntermembrane transport protein PqiB
Reads supporting (aligned to +/- strand):  ref base A (10/6);  new base C (0/14);  total (10/20)
Fisher's exact test for biased strand distribution p-value = 3.00e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CAGCGCGAGCGAATGGGTGAAACGCTGGACGAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCTGATTCGTGGCCGGCCGAAAGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAGACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCGGGGGCTGCAGCAGTACGCCGAACAACTACCACACGTTGGTAC  >  ACB122/2938123‑2938371
                                                                                                                     |                                                                                                                                    
ctgcgcGAGGGATTGGGTGAAAGGCGGGACGAGCTTTAGCTCATGTCGCGTTCGATTCGTGACTTTGCGGATTCCCTGGG‑CCGCCACCCCTAGTCGCTGATTCGGGGCCGGCCTAAAGCGGCCGGGTCCGCAGAc                                                                                                                    <  8:228562/133‑1 (MQ=255)
         ctaaTGGGGCAAACGGTGGACCCGCGGGAGCGCATGTGGCGTGCGCTCCGTCACCTTGCCGATTACGGGGC‑CCGCCACCCCGAGTCGCTTATTCGGGCCGGGCCTAACGCGGCCGGGTCCGCAGACCTTGAAc                                                                                                             <  6:335405/131‑1 (MQ=255)
                gTGAAACGCTGGACGAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGCTTACCTGGG‑CCGCCACCCCGAGTCGCGGATTAGTGGCCCGCCCCAATCCGCCCGGTGCGCCCCCCGTGTACCCAgagggg                                                                                                    >  8:355990/1‑133 (MQ=255)
                gTGAAACGCTGGACGAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCGGCTAAGTTGCCCGCCCAAAGCGGCCCGGGGCGCAGACCGAGAACCCTgagggc                                                                                                    >  6:352859/1‑133 (MQ=255)
                        ggggccGAGGTGGAGCGAATGGCGGGTGCGCTACGTTACGTTGCCGATACCGTGGG‑CCGCCCCCCCGCGGCGGTGTTGCGTGGCGGGCCGAACGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAGACAGCCGa                                                                                            <  6:160353/130‑1 (MQ=255)
                            aCGAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCTGATTCGTGGCCGGCCGCAAGCGGCCCGGTCCGCAGACCTTGCACCCTGAGGAGACAGCCgcagca                                                                                        >  2:29703/1‑130 (MQ=255)
                             cGAGCGGGAGCGAAGGTCGCGTGCGATAGGTGACCTGGCCTAATACGTGGG‑CCGCCCCCCCGCGTCGCTGATGCGGGGCCGCCCCAACGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAGACAGCCGATGCaa                                                                                       <  6:68589/135‑1 (MQ=255)
                                gcgggAGCGAATGGCGCGTGCGCTACGTGACCTGGCCTATTCCGTGGG‑CCGCCACCCCGAGGCGCTGATTCGGGGCCGGCCGAACGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAGACAGCCGATGCAACGa                                                                                    <  2:12134/132‑1 (MQ=255)
                                gCTGGAGCGCATGGCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGGCGCTGATGCGTGGCCGGCCGAAAGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAGACAGCCGATGCAACGa                                                                                    <  8:293783/135‑1 (MQ=255)
                                  gggcgcgcTTGGCGGGTGCGCTACGTGCCCTTGCCGATTCCCGGGC‑CCGCCACCCCGAGGCGCGGATTCGGGGCCGCCCGAACGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAGACAGCCGATGCAACGACt                                                                                  <  6:61967/131‑1 (MQ=255)
                                  gggAGCGCTGGGCGCGTGCGCTACGGGACGTGGCCGATTCCCGGGC‑CCCCCCCCCCGCGGCGGTGATGCGTGGCGGGCCGAAAGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAGACAGCCGATGCAACGACt                                                                                  <  7:33960/134‑1 (MQ=255)
                                  gggAGCGCATGGCGCGTGCGCTCCGGGCCCTTCCCTATTACCGGGC‑CCCCCCCCCCGCGGCGCTGATGCGGGGCCGGCCTAACGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAGACAGCCGATGCAACGACt                                                                                  <  4:268897/134‑1 (MQ=255)
                                            gTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGGCGCTGCTTCGTGGCCGGCCGAAAGCGGCCGGGTCCGCAGCCCGTGAACCCTGAGGAGACAGCCGATGCAACCACTGCGCAAAGCa                                                                        >  8:302492/1‑135 (MQ=255)
                                               gcgTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCTGATTCGTGGCCGGCCGAAAGCGGCCGGGTCCGCAGACCTTGCCCCCTGAGGGGGCCGCCGATGCAACGCCTGCGCAATGGAtgt                                                                     >  7:234166/1‑135 (MQ=255)
                                                 tttggCTAGGTGACCTTGCCTATTACGTGGG‑CCCCCCCCCCGCGGGGGTGATGCGTGGCCGGCCGAAAGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAGACAGCCGATGCAACGACTGCGCAATGCAtgtgt                                                                   <  8:286979/131‑1 (MQ=255)
                                                 tgtCGATACGTCACCTGGCCTATCCCGTGGG‑CCGCCACCCCGTGGCGCTGATGCGGGGCCGGCCTAACGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAGACAGCCGATGCAACGACTGCGCAATGCAtgtgt                                                                   <  3:167977/133‑1 (MQ=255)
                                                    cGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCGGATTCGTGGCCGGCCGCAAGCCGCCCGGGGCGCAGACCGTGCACCCAGGGGAGCCAGCCGATGCAACCACTGCGCAATGCATGGGTggg                                                                >  6:372662/1‑134 (MQ=255)
                                                          gTGCCCTGGCCGA‑TACCGGGGCCCGCCCCCCCGCGGCGCTGTTGCGGGGCCGGCCGAAAGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAGACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGc                                                            <  2:956/133‑1 (MQ=255)
                                                               cTTGACTATTCCCTGGG‑CCCCCACCCGGAGCCGCGTATTGGGGGCCGGCCTAACGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAGACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTgg                                                     <  2:262246/135‑1 (MQ=255)
                                                                          ccTGGG‑CCGCCACCCCGAGTCGCTGATTCGTGGCCGGCCGAAAGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAGACAGCCGCTGCAACGACGGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCggg                                          >  1:34068/1‑135 (MQ=255)
                                                                           cTGGG‑CCGCCACCCCGAGTCGCTGATTCGTGGCCCGCCGAAAGCGGCCCGGGCCGCCGACCTTGAACCCAGAGGACACACCCGAAGCACAGCCAGCGCAATTCATGTGTGGCCAGCCGGCTGGTGTGGCGCgggg                                         >  1:225571/1‑135 (MQ=255)
                                                                                  cgccACCCCGAGTCGCTGATTCGTGGCCGGCCGAAAGCGGCCGGGTCCGCAGACCTTGACCCCTGAGGAGACAGCCGATGCAACGCCTGCGCCATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCGGGGGCTgca                                   >  8:187018/1‑135 (MQ=255)
                                                                                       ccccGAGTCGCTGATTCGTGGCCGGCCGAAAGCGGCCGGGTCCGCAGCCCTTGAACCCTGAGGGGCCCGCCGCTGCAACGCCTGCGCAATGCATGTGTGGCCAGCCGGCTGGTGTGGGTCGGGGGCGGcagcagg                              >  4:122520/1‑134 (MQ=255)
                                                                                          cgtgTCGCTGATTCGGGCCCGGCCGAAAGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAGACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCGGGGGCTGCAGCAGTACg                           <  3:370251/132‑1 (MQ=255)
                                                                                                     ttggTGGCGGGCCGAACGCGGCCGGGTCAGCAGACCTTGAACCCTGAGGAGACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCGGGGGCTGCAGCAGTACGCCGAACAACTa                <  2:225571/132‑1 (MQ=255)
                                                                                                     ttCGTGGCCGGCCCAACGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAGACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCGGGGGCTGCAGCAGTACGCCGAACAACTa                <  1:29703/135‑1 (MQ=255)
                                                                                                     ggcGTGGCCGCCCCAACGCGGCCGGGGCCGCAGACCTTGAACCCTGAGGAGACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCGGGGGCTGCAGCAGTACGCCGAACAACTa                <  1:181679/133‑1 (MQ=255)
                                                                                                          ggccggccTAACGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAGACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCGGGGGCTGCAGCAGTACGCCGAACAACTACcaca           <  4:194323/135‑1 (MQ=255)
                                                                                                          ggccggccGAACGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAGACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCGGGGGCTGCAGCAGTACGCCGAACAACTACcaca           <  7:373763/135‑1 (MQ=255)
                                                                                                          ggccgcccGAACGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAGACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCGGGGGCTGCAGCAGTACGCCGAACAACTACcaca           <  8:160349/135‑1 (MQ=255)
                                                                                                                   aaaGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAGACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCGGGGGCTGCAGCAGTACGCCGAACAACTACCACACGTTGGTAc  >  1:107236/1‑135 (MQ=255)
                                                                                                                     |                                                                                                                                    
CAGCGCGAGCGAATGGGTGAAACGCTGGACGAGCTGGAGCGCATGTCGCGTTCGCTACGTGACCTTGCCGATTACCTGGG‑CCGCCACCCCGAGTCGCTGATTCGTGGCCGGCCGAAAGCGGCCGGGTCCGCAGACCTTGAACCCTGAGGAGACAGCCGATGCAACGACTGCGCAATGCATGTGTGGCCAGCCTGCTGGTGTGGCTCGGGGGCTGCAGCAGTACGCCGAACAACTACCACACGTTGGTAC  >  ACB122/2938123‑2938371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑