| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,210,881 | 0 | T | G | 71.4% | ‑1.1 / 18.6 | 28 | V204G (GTC→GGC) | oprJ_2 | Outer membrane protein OprJ |
| Reads supporting (aligned to +/- strand): ref base T (8/0); new base G (1/19); total (9/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.90e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.09e-05 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCCTGATCGCCGAGGTGAGCCAGGCCTACCTGGCCTTGGACGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGC‑GCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAGCGCCAGGCCTACAACGCGCTGG > ACB122/3210772‑3211009 | gCCCTGATCGCCGAGGTGAGCCAGGCCTACCTGGCCTTGGACGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGCTTCCCTTGCGTTGGTCGGCCAGCCGCGG‑gccccccccccc > 8:354861/1‑129 (MQ=255)gCCCTGATCGCCGAGGTGAGCCAGGCCTACCTGGCCTTGGACGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGC‑Gccgcccgcccc > 2:235136/1‑132 (MQ=255) cGCCGAGGTGAGCCAGGCCTACCTGGCCTTGGACGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGC‑GCCGCCCGccccgccccggc > 2:18264/1‑135 (MQ=255) ggTGAGCCAGGCCTACCTGGCCTTGGACGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGC‑GCCCCCCGCCCCGCCCCCGCGCgggg > 6:334685/1‑134 (MQ=255) cccggCCTCCCTGGCCTGGGCCGGCGCCGCGCGGCGCCCGGCGCTGCCCCGCCCCCCCGGGGCCCCCCGCGAGGATTCCCGGGCGGGGGGCGGCCAGCGGCGC‑GCCGCCGGCACGGCCACGGCGCTGGAccacca < 5:162705/132‑1 (MQ=255) cccggCCTACCTGGCCTTGGCCGGCGCCGAGGGGCGCCAGGGGCTGACCCGGCAGCCCCTGGCCAGCCGCGAGGATGCCCGGGCGTGGGGCGGCCAGCGGCGC‑GCCGCCGGCACGGCCACGGCGCTGGAccacca < 3:178990/132‑1 (MQ=255) cAGGCCTACCTGGCCTTGGACGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGCTTCCCTGGCGTTGGGCGGCCAGCGGCGC‑GCCCCCCGCCCGGCCCCGGCGCGGGccccccca > 2:366796/1‑133 (MQ=255) tGGACGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGCGGCCCCCGCCCCGGCC‑CCGCGCGGGGCCCCCCACCGGCGCGgggggggg > 5:313518/1‑131 (MQ=255) tGGACGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGC‑GCCCCCCGCCCCGCCCCGGCGCTGGGCCCCCAACACGCGCTGGGGCggg > 7:94348/1‑135 (MQ=255) tGGACGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGC‑GCCCCCCGCACCGCCCCCGCGCGGGGCCACCAAGAGGCGCTGGGGCggg > 7:268199/1‑135 (MQ=255) gccGGCGCCGCGCGGCGCCAGGCGCGCACCCGTCAGACCGGGGCCCGCGGCGAGGCTTCCCGGGGGTTGGGCGGCCAGCGGCGC‑GCCGCGGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTc < 7:109470/133‑1 (MQ=255) gagggCGCCGAGCGGCGCCCGGCGCGGACCCGGCAGCCCCTGCCCAGCCGCGAGGATTCCCGGGCGTTGGGCGGCCAGCGGCGC‑GCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTc < 6:313518/132‑1 (MQ=255) gACGGCGCCGCGCGGCGCCAGGCGCGGACCCGTCACACCCGGGCCCGCCGCGAGGATTCCCTGGCGGTGGGCGGCCAGCGGCGC‑GCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTc < 2:287530/135‑1 (MQ=255) aCGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGATGCCCGGGCGTTGGGCGGCCAGCGGCGC‑GCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTc < 2:88435/134‑1 (MQ=255) gggCGCCGCGGGGCGCCCGGCGCGGCCCCGCCCCCCCCGGCCCCCCCGCGAGGATGCCCGGGGGTGGGGCGGCCCGCGGCGC‑GCCGCCGGCAGGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGa < 3:17676/134‑1 (MQ=255) gcCGCGCGGCGCCAGGCGCTGCCCCGTCAGCCCCTGGCCAGCCGCGTGGATTCCCTGGCGTTGGGCGGCCAGCGGCGC‑GCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAg < 4:110894/135‑1 (MQ=255) gcggcgCCCGGCGCTGCCCCGCCCGCCCCTGGCCCGCCGCGAGGATCCCCGGGCGTGGGGCGCCCCGCGGCGC‑GCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCg < 4:5711/135‑1 (MQ=255) gcggcgCCCGGCGCTGACCCGGCCGACCCTGGCCCGCCGGGAGGATTCCGTGGCGTGGGGCGGCCCGCGGCGC‑GCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCg < 8:49749/135‑1 (MQ=255) cggcgCCAGGCGCTGACCCGTCAGCCCCGGGCCAGCCGCGAGGATGCCCGGGCGTGGGGCGGCCAGCGGCGC‑GCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGt < 7:182342/135‑1 (MQ=255) gCCCGCCGCGAGGATGCCGGGGCGTGGGGCGCCCCGCGGCGC‑GCCGCCGGCCCGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAg < 8:35409/135‑1 (MQ=255) gCCAGCCGCGTGGTTTCGCGGGGGTGGGGCTGCCCGGGGGGC‑GCCGCCGGCAGGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAg < 6:351989/135‑1 (MQ=255) gCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGC‑GCCGCCCGCACCGCCACGGCGCGGGGCCACCAAGAGGCGCTCGGGCTGGTCGCGCACTCCCCTGCCCACCTCGAAGGCACCCTGcccccaaac > 6:163594/1‑130 (MQ=255) ccGGGAGGAGTCCGTGGCGGTGGGCGGCCAGCGGCGC‑GCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAGCGCCa < 8:268199/135‑1 (MQ=255) cgcgAGGATTCCCTGGCGTGGGGCGGCCAGCGGCGC‑GCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAGCGCCAg < 6:376078/135‑1 (MQ=255) atgcccGGGCGGGGGGCGGCCCGCGGCGC‑GCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGGCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAGCGCCAGGCCTACa < 3:363199/132‑1 (MQ=255) aTTCCCGGGGGTGGGGCGGCCCGCGGCGC‑GCCGCCGCCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGGCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAGCGCCAGGCCTACa < 2:74743/135‑1 (MQ=255) cggtGGGCGGCCTGCGGCGC‑GCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCGGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAGCGCCAGGCCTACAACGCGCggg < 3:328611/132‑1 (MQ=255) cGTGGGGCGGCCAGCGGCGC‑GCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAGCGCCAGGCCTACAACGCGCTgg < 4:83031/135‑1 (MQ=255) | GCCCTGATCGCCGAGGTGAGCCAGGCCTACCTGGCCTTGGACGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGC‑GCCGCCGGCACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAGCGCCAGGCCTACAACGCGCTGG > ACB122/3210772‑3211009 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |