| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 5,223,159 | 0 | G | C | 55.6% | ‑4.2 / 16.2 | 27 | intergenic (‑133/+43) | cntO_7/CJ019_04917 | Metal‑pseudopaline receptor CntO/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (3/9); new base C (13/2); total (16/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.95e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGT‑AAACCCGCCCCTA‑C‑GGGGGGTTTACCCGTG‑AAGAGGC‑TGGCACAGGCAACTACCGAACC‑AGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCG > ACB122/5223028‑5223284 | gACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGT‑AAAcccccc > 4:21963/1‑134 (MQ=255) aGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGT‑TAACACCCCCCAA‑C‑g > 6:167930/1‑135 (MQ=255) aCCTGTGAGGGGGTGATTATTGCGGATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGT‑AAACCCCCCCCTA‑C‑cgg > 8:126611/1‑135 (MQ=255) aCCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCACATCGC‑GGGT‑TAAAACCCCCCCA‑C‑gcg > 5:133534/1‑133 (MQ=255) tgtgAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGT‑TAAACCCCCCCCA‑A‑gggggg > 2:48494/1‑135 (MQ=255) gggggTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGT‑TAAACCCCCCCCA‑C‑GGGGGGgttta > 4:150406/1‑133 (MQ=255) gggggTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGG‑TAACCCCCCCCCA‑C‑cgggggggtta > 2:123417/1‑130 (MQ=255) tGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGTAAAACCCCCCCCAA‑C‑GGGGGGTTTAccgt > 8:214462/1‑132 (MQ=255) tGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGT‑TAACCCGCCCCGA‑C‑GGGGGGGTTTCCCg > 4:36743/1‑133 (MQ=255) tGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGT‑AAACCCGCCCCGA‑C‑GGAGGGTTTACCCGt > 4:45214/1‑134 (MQ=255) tGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGT‑AAAACCCCCCCCA‑C‑GGGGGGGTTTCCCgg > 1:49587/1‑133 (MQ=255) attattGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGT‑TAACCCGCCCCAA‑C‑GGGGGGGTTACCCCTgca > 8:304819/1‑133 (MQ=255) attattGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGT‑AAACCCCCCCCTC‑C‑GGGGGGGTTTCCCGTgta > 8:320099/1‑133 (MQ=255) attattGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTCCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGGT‑AAACCCCCCCCAC‑C‑GGGGGGTTTGCCCgggt > 7:53770/1‑132 (MQ=255) ccaatcaagcGCACTTACACTCATAACCCGCAGGCCAGGGGCTGTACCCTCACCGGTGCAGGTAAATATTTTGGCGGTGGCGGCCGCTCCGC‑GGGA‑AAACCCGCCCCTA‑C‑GGGGGGTTAACCGGGT‑AAGTGGC‑Tg < 6:377430/133‑1 (MQ=255) aCTCATTACACGGATGCCCCGGTCTTTTACCGCACCTGTGCCTGGAAATTTTTTTGCGGGGGCGGCCTGTTCGC‑GGGT‑AAACCCGCCCCTA‑C‑GGGGGGTTTACCCGTG‑AAGAGGC‑TGGCACAGGCAACTACCGaa < 8:282776/135‑1 (MQ=38) cACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGT‑TAACCCCCCCCCA‑CAGGGGGGTTTACCCG‑G‑GACAGGC‑CTGGACAGGCAACAACCGCACCAAGGGCCAAACcccc > 6:358868/1‑135 (MQ=255) cACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGT‑TAACACCCCCCTA‑C‑GGGGGGGGTACCCGGG‑AAGAGGC‑TGGCCCCGGCAACTACCCAACC‑AGGGGCACACcccc > 8:51016/1‑134 (MQ=255) cccggggCCTGCAAATATTTTTGCTGGGGCGGCCGCTTCGC‑GGGA‑AAACCCGCCCCTC‑G‑GGGGGGTTAACCCGTG‑AAGTGGC‑TGGCACAGGCAACTACCGAACC‑AGGGTCAAACGCCCCAACACGGTCTGCCGTg < 8:126624/129‑1 (MQ=255) aCCTGTGCCTGGAAATATTTTTGCTGGGGCGGCCTCTTTGC‑GGGA‑AAACCCGCCCCTC‑C‑GGGGGGTTTACCCGTG‑AAGAGGC‑TGGCACAGGCAACTACCGAACC‑AGGGTCAAACGCCCCAACACGGTCTGCCGTg < 7:356796/135‑1 (MQ=255) cgtgtgCCTGCAAATATTTTTGCGGGGGCGGCCTGTTCGC‑GGGA‑ACACCCGCCCCTC‑C‑GGGGGGTTAACCGGTG‑AAGAGGC‑TGGCACAGGCAACTACCGAACC‑AGGGTCAAACGCCCCAACACGGTCTGCCGt < 8:225535/131‑1 (MQ=255) ccctccaaaTATTTTGGGGGGGGGGGCCTC‑TCCC‑GGGA‑AAACCCCCCCCCACC‑GGGGGGTTAACCGGGG‑AAGTGGC‑GGGCACAGGCAACTACCGAACC‑AGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAAc < 5:376399/129‑1 (MQ=255) cTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGT‑‑AACCCCCCCCTA‑C‑CGGGGGTTTAACCGGG‑AAGCGGC‑GGGCACCGGCAACTACCGAACC‑AGGGTCAAACGCCCCAACACGGTCTGCCGGGCAAAACCCa > 8:27627/1‑135 (MQ=255) tatTTTTGCTGGGGGGGCCGCTTCGC‑GGGT‑AACCCCGCCCATA‑C‑GGGGGGTTACCCCGGT‑AAGTGGC‑TGGCACAGGCAACTACCGAACC‑AGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCt < 8:76059/135‑1 (MQ=255) tatTTTTGCTGGGGGGGCCGCTGCGC‑GGGT‑AAACCCGCCCCTA‑G‑GGGGGGTTTACCGGGT‑AAGAGGC‑TGGCACAGGCAACTACCGAACC‑AGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCt < 3:172380/135‑1 (MQ=255) tgggggggcCTCTTCGG‑GGGT‑AACCCCGCCCCTA‑G‑GGGGGGTTAACCCGTG‑AAGAGGC‑TGGCACAGGCAACTACCGAACC‑AGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCg < 2:49587/129‑1 (MQ=255) tgcgc‑gGGT‑ACACCGGCCCCCT‑G‑GGGGGGTGTACCGGGT‑ACGTGGC‑GGGCACAGGCAACTACCGAACC‑AGGGTCAACCGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCca < 7:182369/133‑1 (MQ=255) gccaaCCCCGCCCTA‑C‑GGGGGGTTTACCCGGGCACTCGGCGTGCCAC‑GGCAACTACCGAAGC‑AGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTCCCCAGCGAATCCAGCGCCAGCAGCg < 8:204195/132‑1 (MQ=255) | GACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGT‑AAACCCGCCCCTA‑C‑GGGGGGTTTACCCGTG‑AAGAGGC‑TGGCACAGGCAACTACCGAACC‑AGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCG > ACB122/5223028‑5223284 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |