Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB122 1,179,510 C→T S554S (AGC→AGT)  purL → Phosphoribosylformylglycinamidine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,179,5100CT88.4% 112.7 / 6.1 43S554S (AGC→AGT) purLPhosphoribosylformylglycinamidine synthase
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base T (19/19);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

CCTGAGCTGGTCAACGACGGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGCGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGCACCTGACCGTCAGCGACAGCCACTTCGGCAACACCCCGGTGG  >  ACB122/1179379‑1179637
                                                                                                                                   |                                                                                                                               
ccTGAGCTGGTCAACGACGGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGtgcg                                                                                                                              <  5:142845/135‑1 (MQ=255)
 cTGAGCTGGTCAACGACAGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCCGGCATGGCCCCGCACGAAAACTGGAGCAACCAATCGCAAGAGCGTTACCTGCTGGACGGCAGTGCgg                                                                                                                             >  2:185526/1‑135 (MQ=255)
  tGAGATGGTCAACGACGGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATAGCAAGCTCGATACGTGATGGCCGTCAGTGCGGt                                                                                                                            >  6:252499/1‑135 (MQ=255)
       tGGTCAACGACGGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGCAATCTGGAGCAACGCATCGCAAGAGCGTTACTTGCTGGCCGTCAGTGCGGTCGAcg                                                                                                                       >  8:307745/1‑134 (MQ=255)
         ggccacgacgGGGGCAGCGGGGGTCGCATCTAGCGGCGCAACGTGACCAAAGACGAGCCGGGCATGGACCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTc                                                                                                                     <  1:378233/131‑1 (MQ=255)
         gTCAACGACGGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACtt                                                                                                                      >  3:355255/1‑134 (MQ=255)
         gTCAACGACGGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTc                                                                                                                     <  3:110897/135‑1 (MQ=255)
         ctCAACGACGGTTGCCGCGGTGGTCTCTTAGAGCTGCGCAACGTGCCCATCGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTc                                                                                                                     <  1:221248/134‑1 (MQ=255)
          tCAACGACGGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTc                                                                                                                     <  1:347619/134‑1 (MQ=255)
          tCAACGACGGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAAACGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTc                                                                                                                     <  8:9537/134‑1 (MQ=255)
            aaCGACGGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTc                                                                                                                     >  4:98040/1‑132 (MQ=255)
            aaCGACGGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTc                                                                                                                     <  3:98040/132‑1 (MQ=255)
            aaCGACGGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAg                                                                                                                  >  2:91643/1‑135 (MQ=255)
            aaCGACGGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAg                                                                                                                  >  6:316787/1‑135 (MQ=255)
            aaCGACGGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGCGCGGTCGACTTCGAg                                                                                                                  >  8:286259/1‑135 (MQ=255)
                     ggCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGCGCGGTCGACTTCGAGCGCTTCAAg                                                                                                         >  5:364322/1‑135 (MQ=255)
                      gCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACtt                                                                                                                      <  3:171088/121‑1 (MQ=255)
                      gCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACtt                                                                                                                      >  4:171088/1‑121 (MQ=255)
                      gCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCATCAAgg                                                                                                        >  5:317589/1‑135 (MQ=255)
                         gcgGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCGTCAAGGccc                                                                                                     >  5:38621/1‑134 (MQ=255)
                                              gcAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTg                                                                                  <  5:93256/133‑1 (MQ=255)
                                                aaCGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGCGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCg                                                                              >  3:67886/1‑135 (MQ=255)
                                                     gCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGc                                                                         >  1:292491/1‑135 (MQ=255)
                                                        cAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGCACGTTGCCCGTTTGCCgt                                                                      >  5:290378/1‑135 (MQ=255)
                                                                   cGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAg                                                           >  4:348735/1‑135 (MQ=255)
                                                                        aTGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGAc                                                       >  8:220756/1‑134 (MQ=255)
                                                                            ccccGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTgaag                                                  <  1:91643/135‑1 (MQ=255)
                                                                            ccccGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTgaa                                                   <  8:176180/134‑1 (MQ=255)
                                                                             cccGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGCGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTgaaga                                                 <  4:67886/135‑1 (MQ=255)
                                                                               cGCACGAACTCTGGCGCAACGAATCTCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTAAAGGCCATCTGCGAGCGAGAACTTTTCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAAc                                               <  3:53325/135‑1 (MQ=255)
                                                                                 cACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAg                                             <  4:277974/135‑1 (MQ=255)
                                                                                         cTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGCACCTGAc                                     >  7:288810/1‑135 (MQ=255)
                                                                                              gCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGCACCTGACCGTCa                                <  7:367067/135‑1 (MQ=255)
                                                                                               cAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGCACCTGACCGTCAg                               >  2:84918/1‑135 (MQ=255)
                                                                                                    aaTCGCAAGCCCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTGGCCCGTTTGCCGTGGGGGGTGAAGCGAGTGAAGAACAGCACCTGACCGTCAGCGACa                          <  2:195881/135‑1 (MQ=255)
                                                                                                           agagCGTTACGTGCTGGCCGTCAGTGCGGGCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTGGCCGTGGTGGGTGAAGCGACTGAAGAACAGCACCTGACCGTCAGCGACAGCCACtt                   <  3:182161/135‑1 (MQ=255)
                                                                                                           agagCGTTACGTGCTGGCCGTCAGCGCGGTCGACTTCGAGGGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGCACCTGACCGTCAGCGACAGCCACtt                   <  7:286259/135‑1 (MQ=255)
                                                                                                              gCGTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGCACCTGACCGTCAGCGACAGCCACTTCgg                <  7:248042/135‑1 (MQ=255)
                                                                                                                gTTACGTGCTGGCCGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGCACCTGACCGTCAGCGACAGCCACTTCGGCa              <  1:84918/135‑1 (MQ=255)
                                                                                                                      tGCTGGCCGTCAGTGCGGTCGACTTCGCGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGCACCTGCCCGTCAGCGACCGCCACTTCGGCAACAccc        >  3:208369/1‑135 (MQ=255)
                                                                                                                            ccGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGCGCGCGAACGTTGCCCGTTTGCCGTGGGGGGGGAAGCGACTGAAGAACAGCACCTGACCGTCAGCGACAGCCACTTCGGCa              <  2:64286/123‑1 (MQ=255)
                                                                                                                            ccGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGCCTGAAGAACAGCACCTGACCGTCAGCGCCAGCCACTTCGGCAACACCCCGGTgg  >  3:14009/1‑135 (MQ=255)
                                                                                                                            ccGTCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGCACCTGACCGTCAGCGACAGCCACTTCGGCa              >  1:64286/1‑123 (MQ=255)
                                                                                                                            ccGGCAGTGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGCACCTGACCGTCAGCGACAGCCACTTCGGCAACACCCCGGTgg  <  5:252499/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                               
CCTGAGCTGGTCAACGACGGTGGCCGCGGTGGTCGCTTCGAGCTGCGCAACGTGCCCAACGACGAGCCGGGCATGGCCCCGCACGAAATCTGGAGCAACGAATCGCAAGAGCGTTACGTGCTGGCCGTCAGCGCGGTCGACTTCGAGCGCTTCAAGGCCATCTGCGAGCGCGAACGTTGCCCGTTTGCCGTGGTGGGTGAAGCGACTGAAGAACAGCACCTGACCGTCAGCGACAGCCACTTCGGCAACACCCCGGTGG  >  ACB122/1179379‑1179637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑