Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB122 1,806,928 G→T G76V (GGG→GTG)  yeiG → S‑formylglutathione hydrolase YeiG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,806,9280GT89.0% 182.3 / 13.1 73G76V (GGG→GTG) yeiGS‑formylglutathione hydrolase YeiG
Reads supporting (aligned to +/- strand):  ref base G (5/3);  new base T (34/31);  total (39/34)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.88e-01

CGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTGGGCCCAGCACT  >  ACB122/1806797‑1807059
                                                                                                                                   |                                                                                                                                   
cGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGGGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCt                                                                                                                                  <  4:7551/135‑1 (MQ=255)
 gCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGcgg                                                                                                                                 >  3:8441/1‑133 (MQ=255)
 gCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTg                                                                                                                                 >  2:99256/1‑135 (MQ=255)
 gCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTg                                                                                                                                 >  3:133291/1‑135 (MQ=255)
 gCTGTGTACCTGCCGCCGCAGGACGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACAGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGcgg                                                                                                                                 >  5:26534/1‑133 (MQ=255)
        aCCTGCCGCGGCTGGCGTAGCAGGTCGAAAAGCTGCCGTTTCTTTTCTGGCTCAGCGGCCTAACCTCCACCGACGAGAACTTCATGCCGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCAtcg                                                                                                                          <  4:295437/135‑1 (MQ=255)
        aCCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGatcatc                                                                                                                           <  3:168190/134‑1 (MQ=255)
        aCCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCAtcg                                                                                                                          <  6:383323/135‑1 (MQ=255)
              cgccgcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGc                                                                                                                      <  1:310561/133‑1 (MQ=255)
              cgccgcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGc                                                                                                                      <  7:191859/133‑1 (MQ=255)
              cgccgcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGc                                                                                                                      <  7:94209/133‑1 (MQ=255)
               gccgcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGCCGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGcccc                                                                                                                   >  6:313/1‑135 (MQ=255)
                ccgcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGccccc                                                                                                                  >  4:232327/1‑135 (MQ=255)
                ccgcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGcccc                                                                                                                   >  8:198607/1‑134 (MQ=255)
                ccgcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGcccc                                                                                                                   >  1:298186/1‑134 (MQ=255)
                  gcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCg                                                                                                                 >  3:18025/1‑134 (MQ=255)
                    cggCCGAGCAGGGCGAAAAGCTGCCGGTGCTCTACTCGCTCAGCGGCATCACCTGCACCGACGAGAAATTCATTCAGAATGCCGGCGCCCAGCGTCTGTCCGCCGAGCTGGTGCTCCTCATCGTCGCCCCCGATa                                                                                                              >  4:321928/2‑135 (MQ=255)
                                  gAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGc                                                                                                >  3:23957/1‑135 (MQ=255)
                                   aaaaGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCg                                                                                               <  7:277304/135‑1 (MQ=255)
                                   aaaaGCTGCCGGGGCTGTACTGGCGCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCg                                                                                               <  6:332131/135‑1 (MQ=255)
                                       gCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCCGCCCGCGTGGCGAGCa                                                                                           >  2:249706/1‑135 (MQ=255)
                                            cGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGatcatc                                                                                                                           >  8:179958/1‑98 (MQ=255)
                                            cGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGatcatc                                                                                                                           <  7:179958/98‑1 (MQ=255)
                                              gTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCg                                                                                    >  1:85499/1‑135 (MQ=255)
                                                       tGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCg                                                                           <  3:175097/135‑1 (MQ=255)
                                                          cTCAGCGGCCTCACCTCCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCgg                                                                          <  4:240738/133‑1 (MQ=255)
                                                            cAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGCGCAGGTGCCGGGCGCTCCGGACgg                                                                      >  1:337256/1‑135 (MQ=255)
                                                            cAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACgg                                                                      >  7:107103/1‑135 (MQ=255)
                                                            cAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACgg                                                                      >  1:28511/1‑135 (MQ=255)
                                                            cAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACgg                                                                      >  1:200770/1‑135 (MQ=255)
                                                            cAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACgg                                                                      >  4:220240/1‑135 (MQ=255)
                                                                 gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCAtcgtc                                                                                                                        <  2:294205/80‑1 (MQ=255)
                                                                 gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCAtcgtc                                                                                                                        >  1:294205/1‑80 (MQ=255)
                                                                 gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGc                                                                                                                      <  3:251034/82‑1 (MQ=255)
                                                                 gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGc                                                                                                                      >  4:251034/1‑82 (MQ=255)
                                                                 gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGCCGGCGCCt                                                                 >  5:100547/1‑135 (MQ=255)
                                                                 gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCt                                                                 >  4:6414/1‑135 (MQ=255)
                                                                 gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCt                                                                 >  4:25078/1‑135 (MQ=255)
                                                                 gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCCTGGCGAGCAGGTGCCGGGCGCTCCGGACGGCGccg                                                                 >  7:164397/1‑134 (MQ=255)
                                                                 gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGAGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCt                                                                 >  8:257519/1‑135 (MQ=255)
                                                                       ccTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACt                                                           >  3:271910/1‑135 (MQ=255)
                                                                              cgacgaGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCt                                                    >  6:32156/1‑135 (MQ=255)
                                                                               gacgaGAACTTCATGCAGAAGGACGGCGCCCAGCGTCTGGCCGCCGAACAGGTGCAGATAATCGTCGCCCCCGATACACGCCCGCGTCGCGCGCAGGTGCCGGGCGATCCGGACGGCCCCTGGGACGTCGGCCTg                                                   >  6:243676/1‑135 (MQ=255)
                                                                                acgaGAACTTCATGCAGACGGCCGGCGCCCAGGGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTgg                                                  <  6:36424/135‑1 (MQ=255)
                                                                                 cgaGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTggg                                                 >  1:110904/1‑135 (MQ=255)
                                                                                   agaACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGgcg                                               >  8:34916/1‑135 (MQ=255)
                                                                                   agaACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGgcg                                               >  1:22260/1‑135 (MQ=255)
                                                                                   agaACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGGGGCGCGCAGGTGCCGGGCGCTCCGGACGGCGCCGGGGCCTTCGGCCAGGgcg                                               >  2:198148/1‑135 (MQ=255)
                                                                                       cTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGGCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTgg                                           <  6:313100/135‑1 (MQ=255)
                                                                                        ttCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGc                                          <  5:32156/135‑1 (MQ=255)
                                                                                        ttCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGc                                          <  7:125605/135‑1 (MQ=255)
                                                                                        ttCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGc                                          >  8:6152/1‑135 (MQ=255)
                                                                                                  aGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCa                                >  6:2711/1‑135 (MQ=255)
                                                                                                     ccGGCGCCCAGCGTCTGGCCGGCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGGAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACg                             <  2:270643/135‑1 (MQ=255)
                                                                                                     ccGGCGCCCAGCGGCTGGCCGCCGATCGGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACg                             <  6:50905/135‑1 (MQ=255)
                                                                                                          gcCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCAcc                        <  2:22260/135‑1 (MQ=255)
                                                                                                          gcCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCAcc                        <  7:34916/135‑1 (MQ=255)
                                                                                                            ccAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCa                      >  5:357832/1‑135 (MQ=255)
                                                                                                                 gTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAAc                 >  8:195961/1‑135 (MQ=255)
                                                                                                                 gTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAAc                 <  2:85499/135‑1 (MQ=255)
                                                                                                                 gTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAAc                 <  6:357832/135‑1 (MQ=255)
                                                                                                                    tGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCt              <  1:99256/135‑1 (MQ=255)
                                                                                                                    tGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCt              <  3:110640/135‑1 (MQ=255)
                                                                                                                      gccgccGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTgg            <  4:18025/135‑1 (MQ=255)
                                                                                                                      gccgccGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTgg            <  3:25078/135‑1 (MQ=255)
                                                                                                                      gccgccGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTgg            <  8:107103/135‑1 (MQ=255)
                                                                                                                       ccgccgAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTgg            <  7:257519/134‑1 (MQ=255)
                                                                                                                        cgccgAGCTGGTGCTGATCATCGTCGCCCCCGATACGAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTGGGcc         <  2:298186/135‑1 (MQ=255)
                                                                                                                            gAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTGGGCCCAg      <  3:6414/135‑1 (MQ=255)
                                                                                                                             aGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGgcgc                                                                   <  5:265835/73‑1 (MQ=255)
                                                                                                                             aGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGgcgc                                                                   >  6:265835/1‑73 (MQ=255)
                                                                                                                             aGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACATCAACGCCACCCCGCCACCCGGGGCCCAGc     >  1:111092/1‑135 (MQ=255)
                                                                                                                                tGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTGGGCCCAGCACt  <  7:195961/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                   
CGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTGGGCCCAGCACT  >  ACB122/1806797‑1807059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: