Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB122 3,065,799 T→G F150V (TTT→GTT)  rcsC_6 → Sensor histidine kinase RcsC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,065,7990TG96.0% 167.7 / ‑1.2 50F150V (TTT→GTT) rcsC_6Sensor histidine kinase RcsC
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base G (35/13);  total (37/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

TACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGTTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGTACCGATGAATTGCTGGAGGCGCGTGATGCCGCCGAAGCCGCC  >  ACB122/3065669‑3065927
                                                                                                                                  |                                                                                                                                
tACCGCGGGGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTCCGGCGCAAGGACGGCAGCCATCTCGATGTGATGATGAACCTGCTGCGCAAACCCGGCCATGCAGGGCTGGTGGAGGGGGTTGt                                                                                                                              <  8:143863/135‑1 (MQ=255)
  ccGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGTTTGTCg                                                                                                                            >  5:163007/1‑135 (MQ=255)
  ccGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCg                                                                                                                            >  6:7002/1‑135 (MQ=255)
  ccGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCAGGAAGGGCTGGTGGAGGGGGTTGTCg                                                                                                                            >  2:188640/1‑135 (MQ=255)
          cTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGTTTGTCGCCGACAtt                                                                                                                    >  2:16891/1‑135 (MQ=255)
          cTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACAtt                                                                                                                    >  2:146883/1‑135 (MQ=255)
          cTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACAtt                                                                                                                    >  7:72321/1‑135 (MQ=255)
          cTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACAtt                                                                                                                    >  7:77105/1‑135 (MQ=255)
          cTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACAtt                                                                                                                    >  2:95625/1‑135 (MQ=255)
             gCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTAcc                                                                                                                 >  3:278249/1‑135 (MQ=255)
                    aGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCa                                                                                                          <  4:156367/135‑1 (MQ=255)
                     gCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCaa                                                                                                         >  4:379096/1‑135 (MQ=255)
                        caGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCt                                                                                                      >  3:174801/1‑135 (MQ=255)
                            cTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGCCATTACCGAACGCAAGCTGGcc                                                                                                  >  5:357808/1‑135 (MQ=255)
                            cTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGcc                                                                                                  >  2:338866/1‑135 (MQ=255)
                            cTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGc                                                                                                   >  6:258510/1‑134 (MQ=255)
                              gCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCca                                                                                                >  8:287776/1‑135 (MQ=255)
                                aCGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGGGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCca                                                                                                <  2:90134/133‑1 (MQ=255)
                                      aCGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGACCCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCCAGCTGGCCCAGCAGCGcc                                                                                        >  5:19639/1‑135 (MQ=255)
                                        gAAACCCGTCTGCGGCGCAAGGCCGGCAGCCATCTCGATGTGCTGATGAACCTGCTCCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGGTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCt                                                                                       <  3:346311/134‑1 (MQ=255)
                                        gAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCt                                                                                       >  2:12036/1‑134 (MQ=255)
                                        gAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTg                                                                                      >  2:309592/1‑135 (MQ=255)
                                        gAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTg                                                                                      >  5:338606/1‑135 (MQ=255)
                                        gAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTg                                                                                      <  6:297591/135‑1 (MQ=255)
                                          aaCCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGca                                                                                    >  1:217428/1‑135 (MQ=255)
                                                 cTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACt                                                                              >  2:56274/1‑134 (MQ=255)
                                                        gcAAGGACGGCAGCCATCTCGATGTGCTTATGAACCTGCTGCTCAAACCCGGCCATGTAGGGCTTGGGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGAct                                                                      <  1:5868/135‑2 (MQ=255)
                                                          aaGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGaa                                                                    <  7:343094/135‑1 (MQ=255)
                                                            ggACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGACCGCAAGCTGGCCCAGCAGCGCCTGCCACAACTCAATGACGCACt                                                                  >  6:60338/1‑135 (MQ=255)
                                                            ggACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACt                                                                  >  5:8159/1‑135 (MQ=255)
                                                               cGGCAGCCTTGTCTATGTGGTGATGAACCTGCGGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGGGGGCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGa                                                               <  6:274579/135‑1 (MQ=255)
                                                                    gCCATCTCGAGGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGACCGCAAGCTGGCCAAGCCTCGCCTGCAACAACTCAATTAAGAACTGGAGAAAc                                                          >  8:368623/1‑135 (MQ=255)
                                                                          tcGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTtg                                                    >  1:224749/1‑135 (MQ=255)
                                                                           cGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTtgc                                                   >  3:167768/1‑135 (MQ=255)
                                                                                   tgatgaACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGACGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGTa                                           <  1:338866/135‑1 (MQ=255)
                                                                                     atgaACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGTAc                                          >  4:15338/1‑134 (MQ=255)
                                                                                       gaACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGTACCGa                                       >  3:371558/1‑135 (MQ=255)
                                                                                           ctgctcCCCAAACAGGGCCATGAAGGGCTGGGGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGTACCGATGaa                                   <  2:370294/135‑1 (MQ=255)
                                                                                             gctgctCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGATCGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGTACCGATGAAtt                                 >  6:71134/1‑135 (MQ=255)
                                                                                               tgctCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGTACCGATGAATTGc                               >  7:43025/1‑135 (MQ=255)
                                                                                                  tCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACACCTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGTACAGATGAATTGCt                              >  6:250838/1‑133 (MQ=255)
                                                                                                      aCCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGTACCGATGAATTGCTGGAGgc                        <  4:278249/135‑1 (MQ=255)
                                                                                                        ccGGCCATGAAGGGCTGGTGGAGGGGGTAGTCGCCGACAATACCGAACGCAAGCTGGCACAGCAGCGCCTGCAACACCTCAATGACCAACTGCAGCAACGCGTTGCAGCGCGGACCGATGAATTGCTGGAGgcgc                      >  2:137863/1‑135 (MQ=255)
                                                                                                         cGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACAGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGCGTTGCTGCGCGTACCGATGAATTGCTGGAGGcgcg                     >  4:276929/1‑135 (MQ=255)
                                                                                                          ggCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGTACCGATGAATTGCTGGAGGCGCGt                    <  3:379096/135‑1 (MQ=255)
                                                                                                                         gtggAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACCACTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGTACCGATGAATTGCTGGAGGCGCGTGATGCCGCCGAAgcc     >  2:176327/1‑135 (MQ=255)
                                                                                                                         gtggAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGTACCGATGAATTGCTGGAGGCGCGTGATGCCGCCGAAgcc     >  4:8442/1‑135 (MQ=255)
                                                                                                                         gtggAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGTACCGATGAATTGCTGGAGGCGCGTGATGCCGCCGAAgcc     >  7:186448/1‑135 (MQ=255)
                                                                                                                            gAGGGGGTTGTCGCCGACATTACCGAACGCAATCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGCACTGGAGCAACGGGTTGCTGCGCGTACCGATGAATTGATGGAGGCGCGTGATGCCGCCGAAgccgc   >  6:20494/1‑134 (MQ=255)
                                                                                                                            gAGGGGGTGGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGTACCGATGAATTGCTGGAGGCGCGTGATGCCGCCGAAgccgcc  <  3:378029/135‑1 (MQ=255)
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TACCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGTTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGTACCGATGAATTGCTGGAGGCGCGTGATGCCGCCGAAGCCGCC  >  ACB122/3065669‑3065927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑