Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 907,674 G→A 8.0% A445A (GCG→GCA)  fruA → PTS system fructose‑specific EIIB'BC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122907,6740GA 8.0% 152.5 / 4.0 50A445A (GCG→GCA) fruAPTS system fructose‑specific EIIB'BC component
Reads supporting (aligned to +/- strand):  ref base G (24/21);  new base A (2/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

ACCAATGCCATCCTGCTGGGGCTGTTGCTGGGCGGCATGATGTGCGTCGACCTTGGCGGGCCGATCAACAAGGCCGCCTATGCGTTCTCGGTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAGAGCGAGCGCGAGGCGGGCAAGGCGGCGCTGGCGCTGGGGCTGTGCTTCATCTC  >  ACB122/907543‑907802
                                                                                                                                   |                                                                                                                                
accaATGCCATCCTGCTGGGGCTGTGGCTGGGCGGCATGATGTGCGGCGACCTTGGCGGGCCGATCAACAAGGCCGCCTATGCGTTCTCGGGGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGAcc                                                                                                                               <  4:76971/135‑1 (MQ=255)
   aaTGCCATCCTGCTGGGGCTGTTGCTGGGCGGCATGATGTGCGTCGACCTTGGCGGGCCGATCAACAAGGCCGCCTATGCGTTCTCGGTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATg                                                                                                                            >  4:129797/1‑135 (MQ=255)
   aaTGCCATCCTGCTGGGGCTGTTGCTGGGCGGCATGATGTGCGTCGACCTTGGCGGGCCGATCAACAAGGCCGCCTATGCGTTCTCGGTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATg                                                                                                                            >  7:286491/1‑135 (MQ=255)
   aaTCCCATACAGCTGGGGCAGTTGCTGGGCGGCATGATCTCCTACGACCATGGCGGGCCGATCAACAAGGCCGCCTATCCGTTCTCGGTGGGGCTGCTGGCCTCCTCTAGCTACGCGACAATGGCGCCAACCAtt                                                                                                                            >  4:77050/1‑134 (MQ=255)
     tGCCAACCTGCGGGGGCTGGTGCTGGGCGGCATGATGTCCGTCGACCGTGGCGGGCCGATCAACAAGGCCGCCTATGCGTTCTCGGGGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGgc                                                                                                                          <  1:234940/135‑1 (MQ=255)
        cATCCTGCTGGGGCTGTTGCTGGGCGGCATGATGTGCGTCGACCTTGGCGGGCCGATCAACAAGGCCGCCTATGCGTTCTCGGTGGGGCTGCTGGACTCGTCGAGCTACGCGCCAATGGCGGCGACCATGgccgc                                                                                                                       >  8:152453/1‑135 (MQ=255)
            ctgctgGGGCTGTTGCTGGGCGGCATGATGTGCGTCGACCTTGGCGGGCCGATCAACAAGGCCGCCTATGCGTTCTCGGTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGc                                                                                                                   >  3:292848/1‑135 (MQ=255)
            ctgctgGGGCTGTTGCTGGGCGGCATGATGTGCGTCGACCTTGGCGGGCCGATCAACAAGGCCGCCTATGCGTTCTCGGTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGc                                                                                                                   >  7:158602/1‑135 (MQ=255)
            ctgctgGGGCTGTTGCTGGGCGGCATGATGTGCGTCGACCTTGGCGGGCCGATCAACAAGGCCGCCTATGCGTTCTCGGTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGc                                                                                                                   >  3:209933/1‑135 (MQ=255)
             tgctgGGGCTGTTGCTGGGCGGCATGATGTGCGTCGACCTTGGCGGGCCGATCAACAAGGCCGCCTATGCGTTCTCGGTGGGGCGGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCa                                                                                                                  >  8:238158/1‑135 (MQ=255)
               ctgGGGCTGTTGCTGGGCGGCATGATGTGCGTCGACCTTGGCGGGCCGATCAACAAGGCCGCCTATGCGTTCTCGGTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATg                                                                                                                >  4:55772/1‑135 (MQ=255)
                  gggCTGTTGCTGGGCGGCATGATGTGCGTCGACCTAGGCGGGCCGATCAACAAGGCCGCCTATGCGTTCTCGGTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCAACCATGGCCGCCGGCATCGTg                                                                                                             >  2:58398/1‑135 (MQ=255)
                      tGTTGCTGGGCGGCATGATGTGCGTCGACCTTGGCGGGCCGATCAACAAGGCCGCCTATGCGTGCTCGGTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTgccgc                                                                                                         <  4:149139/135‑1 (MQ=255)
                      tGTTGCTGGGCGGCATGATGTGCGGCGACCTTGGCGGGCCGATCAACAAGGCCGCCTATGCGTTCTCGGTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTgccgc                                                                                                         <  3:242062/135‑1 (MQ=255)
                      tGTTGCTGGGCGGCATGATGTGCGGCGACCTGGGCGGGCCGATCAACAAGGCCGCCTATGCGTTCTCGGGGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTgccgc                                                                                                         <  8:231081/135‑1 (MQ=255)
                                   cATGATGTGCGTCGACCTTGGCGGGCCGATCAACAAGGCCGCCTATGCGTTCTCGGTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTgg                                                                                             >  1:6159/1‑134 (MQ=255)
                                     tgatgTGCGTCGACCTTGGCGGGCCGATCAACAAGGCCGCCTATGCGTTCTCGGTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTa                                                                                          >  3:235931/1‑135 (MQ=255)
                                     tgatgTGCGTCGACCTTGGCGGGCCGATCAACAAGGCCGCCTATGCGTTCTCGGTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTa                                                                                          >  4:285934/1‑135 (MQ=255)
                                        tgtgCGTCGACCTTGGCGGGCCGATCAACAAGGCCGCCTATGCGTTCTCGGTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTg                                                                                       <  4:209933/135‑1 (MQ=255)
                                                gACCTTGGCGGGCCGATCAACAAGGCCGCCTATGCGTTCTCGGTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTc                                                                               >  6:239845/1‑135 (MQ=255)
                                                   cTTGGCGGGCCGATCAACAAGGCCGCCTATGCGTTCTCGGTGGGGCTGCTGGCCTCGGCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGTATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTg                                                                            <  5:101230/135‑1 (MQ=255)
                                                       gCGGGCCGATCAACAAGGCCGCCTATGCGTTCTCGGTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGccc                                                                        >  8:119671/1‑135 (MQ=255)
                                                            ccGTTCAACAAGGCCGCCTATGCGTTCTCGGGGGGGCTGCTGGCCGCGTCGAGCTACGCGCCAATGGCGGCATCCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGgcgc                                                                   <  3:77050/135‑1 (MQ=255)
                                                                   acaaGGCCGCCTATGCGTTCTCGGTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTc                                                             <  2:184460/134‑1 (MQ=255)
                                                                             cTATGCGTTCTCGGTGGGGCTGCTGGCCTCGTCGATCTACGCGCCAATTGCGGCTACCATGGACGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCAACCTTCATGGCCCGGCGCAAGTTCGCCCAgagaga                                                  >  8:145647/1‑135 (MQ=255)
                                                                             cTATGCGTTCTCGGTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAgagcga                                                  >  1:131522/1‑135 (MQ=255)
                                                                               aTGCGTTCTCGGTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAgagcgagc                                                >  5:186322/1‑135 (MQ=255)
                                                                               aTGCGTTCTCGGTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAgagcgagc                                                >  1:163292/1‑135 (MQ=255)
                                                                                       tcGGTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAGAGCGAGCGCGAGGCg                                        <  8:158602/135‑1 (MQ=255)
                                                                                         ggTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGTCGACCATGGCCGCCGGCAAGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTACGCCCAGAGCGAGCGCGAGGCgg                                       >  7:19456/1‑134 (MQ=255)
                                                                                         ggTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAGAGCGAGCGCGAGGCgg                                       >  8:280020/1‑134 (MQ=255)
                                                                                          gTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAGAGCGAGCGCGAGGCggg                                      >  7:256495/1‑134 (MQ=255)
                                                                                            ggggCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAGAGCGAGCGCGAGGCGGGCaa                                   >  1:7454/1‑135 (MQ=255)
                                                                                                ctgctgGCCGCGGCGAGCAACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAGAGCGAGCGCGAGGCGGGCAAggcg                               <  5:49948/135‑1 (MQ=255)
                                                                                                  gctgGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTGGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAGAGCGAGCGCGAGGCGGGCAAggcggc                             <  8:99229/135‑1 (MQ=255)
                                                                                                    tgGCCTGGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGATTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAGAGCGAGCGCGAGGCGGGAAAggcggc                             <  8:213679/133‑1 (MQ=255)
                                                                                                      gCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAGAGCGAGCGCGAGGCGGGCAAGGCggcgctg                         >  7:284629/1‑135 (MQ=255)
                                                                                                       ccTCGGCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAGAGCGAGCGCGAGGCGGGCAAGGCggcgctgg                        <  8:166185/135‑1 (MQ=255)
                                                                                                            tcgAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAGAGCGAGCGCGAGGCGGGCAAGGCGgcgctggcgctg                   <  4:268382/135‑1 (MQ=255)
                                                                                                            tcgAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAGAGCGAGCGCGAGGCGGGCAAGGCGgcgctggcgctg                   <  8:284629/135‑1 (MQ=255)
                                                                                                            tcgAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAGAGCGAGCGCGAGGCGGGCAAGGCGgcgctggcgctg                   <  2:241970/135‑1 (MQ=255)
                                                                                                            tcgAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAGAGCGAGCGCGAGGCGGGCAAGGCGgcgctggcgctg                   >  3:80145/1‑135 (MQ=255)
                                                                                                            tcgAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAGAGCGAGCGCGAGGCGGGCAAGGCGgcgctggcgctg                   <  4:273053/135‑1 (MQ=255)
                                                                                                              gAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAGAGCGAGCGCGAGGCGGGCAAGGCGGCGCTGGCGCTggg                 >  2:197222/1‑135 (MQ=255)
                                                                                                               cgcaccgcgCCAAGGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAGAGCGAGCGCGAGGCGGGCAAGGCGGCGCTGGCGCTgggg                <  2:131522/130‑1 (MQ=255)
                                                                                                                 cTACGCGCCAAGGCCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTGCGCCCAGAGCGAGCGCGAGGCGGGCAAGGCGGCGCTGGCGCTGGGGCt              <  3:22294/135‑1 (MQ=255)
                                                                                                                    cgcgCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAGAGCGAGCGCGAGGCGGGCAAGGCGGCGCTGGCGCTGGGGCtgtg           >  1:190997/1‑135 (MQ=255)
                                                                                                                     gcgcGATTGGCGGCGACCAGGGCCGCCGGCTTGGTGCCGGCGATTGGCGTGGGTATTGCCACCTTCCTGGCCCGGCGCTAGTTCGCCCAGAGGGAGCGGGAGGCGGGCAAGGCGGCGCTGGCGCTGGGGCTGTGc          <  7:152453/135‑1 (MQ=255)
                                                                                                                     gcgcCACTGGCGGCAACCATGGCCGCCGGCATGGGGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAGAGCGAGCGCGAGGCGGGCAAGGCGGCGCTGGCGCTGGGGCtgt            <  1:58398/133‑1 (MQ=255)
                                                                                                                     gcgcCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTGGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAGAGCGAGCGCGAGGCGGGCAAGGCGGCGCTGGCGCTGGGGCtgtg           <  8:37418/134‑1 (MQ=255)
                                                                                                                               cggcgACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAGAGCGAGCGCGAGGCGGGCAAGGCGGCGCTGGCGCTGGGGCTGTGCTTCAtctc  >  2:53678/1‑133 (MQ=255)
                                                                                                                                   |                                                                                                                                
ACCAATGCCATCCTGCTGGGGCTGTTGCTGGGCGGCATGATGTGCGTCGACCTTGGCGGGCCGATCAACAAGGCCGCCTATGCGTTCTCGGTGGGGCTGCTGGCCTCGTCGAGCTACGCGCCAATGGCGGCGACCATGGCCGCCGGCATGGTGCCGCCGATTGGCTTGGGTATTGCCACCTTCCTGGCCCGGCGCAAGTTCGCCCAGAGCGAGCGCGAGGCGGGCAAGGCGGCGCTGGCGCTGGGGCTGTGCTTCATCTC  >  ACB122/907543‑907802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑