Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,451,862 G→A 8.7% P263S (CCG→TCG)  algA_1 ← Alginate biosynthesis protein AlgA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,451,8620GA 8.7% 132.6 / 3.9 46P263S (CCG→TCG) algA_1Alginate biosynthesis protein AlgA
Reads supporting (aligned to +/- strand):  ref base G (18/24);  new base A (2/2);  total (20/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01

GTTGCCGTGGATCATGCAGTTGCGGCTGTCTTGAACCACCACGTCGCCCTTGGTGACGTTGCCGTTGTCGTCTTTCTCGTGCACTTCCCACAGCGACGACCAGCAGCCTACATCGCTCCAGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCATCACCGCATAGTCAATGGAGTTGTCCGGGCAGCAGGCGAAGGTGGCTTCGTCGATGCTCAGTACATCGCCGTCTTCCTGGCTGCGCTCCAG  >  ACB122/1451728‑1451982
                                                                                                                                      |                                                                                                                        
gttgccgtGGATCATGCAGTTGCGGCTGTCTTGAACCACCACGTCGCCCTTGGTGACGTTGCCGTTGTCGTCTTTCTCGTGCACTTCCCACAGCGACGACCAGCAGCCTACATCGCTCCAGCCGGCCGACATCgg                                                                                                                          <  7:136245/135‑1 (MQ=255)
    ccgtGGATCATGCAGTTGCGGCTGTCTTGAACCACCACGTCGCCCTTGGTGACGTTGCCGTTGTCGTCTTTCTCGTGCACTTCCCACAGCGACGACCAGCAGCCTACATCGCTCCAGCCGGCCGACATCGGcacc                                                                                                                      >  2:40720/1‑135 (MQ=255)
            cATGCAGTTGCGGCTGTCTTGAACCACCACGTCGCCCTTGGTGACGTTGCCGTTGTCGTCTTTCTCGTGCACTTCCCACAGCGACGACCAGCAGCCTACATCGCTCCAGCCGGCCGACATCGGCACCACGCAGGc                                                                                                              >  8:103976/1‑135 (MQ=255)
                 aGTTGCGGCTGTCTTGAACCACCACGTCGCCCTTGGTGACGTTGCCGTTGTCGTCTTTCTCGTGCACTTCCCCCAGCGACGACCAGCAGCCTACATCGCTCCAGCCGGCCGACATCGGCACCACGCAGGCACGCt                                                                                                         >  7:35858/1‑135 (MQ=255)
                 aGTTGCGGCTGTCTTGAACCACCACGTCGCCCTTGGTGACGTTGCCGTTGTCGTCTTTCTCGTGCACTTCCCACAGCGACGACCAGCAGCCTACATCGCTCCAGCCGGCCGACCTCGACACCACGCAGGCACGCt                                                                                                         >  8:62759/1‑135 (MQ=255)
                      cGGCTGTCTTGAACCACCACGTCGCCCTTGGTGACGTTGCCGTTGTCGTCTTTCTCGTGCACTTCCCACACCGACCACCAGCAGCCTACATCGCACCAGCCCGCCCCCATCGGCACCACCCAGCCCCCCTGGGtt                                                                                                    >  4:276413/1‑135 (MQ=255)
                       ggCTGTCTTGAACCACCACGTCGCCCTTGGTGACGTTGCCGTTGTCGTCTTTCTCGTGCACTTCCCACAGCGACCCCCACCAGCCAACATCGCTACAGCCGGCCCACAACGGCCCCCCGCCCGCACGCGGGGttt                                                                                                   >  6:40907/1‑135 (MQ=255)
                             cTTGAACCACCACGTCGCCCTTGGTGACGTTGCCGTTGTCGTCTTTCTCGTGCACTTCCCACAGCGACGACCAGCAGCCTACATCGCTCCAGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTtctc                                                                                               <  7:169629/133‑1 (MQ=255)
                              ttGAACCACCACGTCGCCCTTGGTGACGTTGCCGTTGTCGTCTTTCTCGTGCACTTCCCACAGCGACGACCAGCAGCCTACATCGCTCCAGCCGGCCGCCATCGGCACCACGCAGGCCCGCTGGGTTTTCTCCa                                                                                             >  5:136484/1‑134 (MQ=255)
                                  tccaccaCGTCGCCCTTGGTGACGTTGCCGTTGTCGTCTTTCTCGTGGACTTCCGACAGCGACGACCAGCAGCCTACATCGCTCCATCCGGCCGACATCGGCACCACGCAGGCTCTCTGGGTTTTCTCCATCAc                                                                                         <  5:254729/133‑1 (MQ=255)
                                  accaccACGTCGCCCTTGGTGACGTTGCCGTTGTCGTCTTTCTCGTGCACTTCCCACAGCGACGACCAGCAGCCTACATCGCTCCAGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCATCAc                                                                                         <  8:139686/134‑1 (MQ=255)
                                   ccaccaCGTCGCCCTTGGTGACGTTGCCGTTGTCGTCTTTCTCGTGCACTTCCCACAGCGACGACCAGCAGCCTACATCGCTCCAGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCATCACCg                                                                                       <  5:40907/135‑1 (MQ=255)
                                   ccaccaCGTCGCCCTTGGTGACGTTGCCGTGGTCGTCTTTCTCGTGCACTTCCCACAGCGACGACCAGCAGCCTACATCGCTCCAGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCATCACCg                                                                                       <  4:101198/135‑1 (MQ=255)
                                    caccacGTCGCCCTTGGTGACGTTGCCGTTGTCGTCTTTCTCGTGCACTTCCCACAGCGACGACCAGCAGCCTACATCGCTCCAGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCATCACCGc                                                                                      <  1:111507/135‑1 (MQ=255)
                                      ccacGTCGCCCTTGGTGACGTTGCCGTTGTCGTCTTTCTCGTGCACTTCCCACAGCGACGACCAGCAGCCTACATCGCTCCAGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCATCACCGCAt                                                                                    <  3:168413/135‑1 (MQ=255)
                                         cggcGCCCTTGGGGACGTTGCCGTTGTCGGCTTTCTCGTGCACTTCCCACAGCGACGACCAGCAGCCTACATCGCTCCAGCCGGCCGACATCGGCTCCACGCAGGCACACTGGGTTTTCTCCATCACCGCATAGt                                                                                 <  5:44470/132‑1 (MQ=255)
                                         cGTCGCCCTTGGTGACGTTGCCGTTGTCGTCTTTCTCGTGCACTTCCCACAGCGACGCCCAGCAGCCTACATCGCTCCAGCCGGCCGCCATCGGCACCACGCAGGCCCGCTGGGGTTTCTCCATCACCGCATAg                                                                                  >  5:96337/1‑134 (MQ=255)
                                                 ttGGTGACGTTGCCGTTGTCGTGGTTATCGTGGACCTACCAAAGCGACGACCAGCAGCCTACATAGCTACAGCCGGCCGACATCAGCACCCCGCAGGCACGCTGGGTTTTAACCAACACCGGAAAGTCCATGGAg                                                                         >  2:216213/1‑135 (MQ=255)
                                                   ggTGACGTTGCCGTTGTCGTCTTTCTCGTGCACTTCCCACAGCGACGACCAGCAGCCTACATCGCTCCAGCCGGCCGACATAGGCACCCCGCAGGCACGCTGGGTTTTCTCCATCACCGCATAGTCAATGGAGtt                                                                       >  4:153684/1‑135 (MQ=255)
                                                      gACGTTGCCGTTGTCGTCGTTCTCGGGCCCATCCCCCCGCGCCCCCCCCCCGCCGACCACGCTCCCGCCCGCCCCCCTCCGCCCCCCCCCGCCCCCCTGGGGTTTCTCCCTCACCGCATAGTCAATGGACTTGtg                                                                    >  7:118527/1‑134 (MQ=255)
                                                              cGTTGTCGTGTTTCTCGTGCACTTCCCACAGCGACGACCAGCAGCCTACATCGCTCCAGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCATCACCGCATAGTCAATGGAGTTGTCCGGgcag                                                             <  3:276413/134‑1 (MQ=255)
                                                                               tGCACTTCCCACAGCGACGACCAGCAGCCTACATCGCTCCAGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCATCACCGCATAGTCAATGGAGTTGTCCGGGCAGCAGGCGAAGGTGGCTtcg                                           <  6:207033/135‑1 (MQ=255)
                                                                                    ttCCCACAGCGACGACCAGCAGCCTACATCGCTCCAGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCATCACCGCATAGTCAATGGAGTTGTCCGGGCAGCAGGCGAAGGTGGCTTCGTCGAt                                      <  5:15471/135‑1 (MQ=255)
                                                                                         acaGCGACGACCAGCAGCCTACATCGCTCCAGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCATCACCGCATAGTCAATGGAGTTGTCCGGGCAGCAGGCGAAGGTGGCTTCGTCGATGCTCa                                 >  4:273230/1‑135 (MQ=255)
                                                                                         acaGCGACGACCAGCAGCCTACATCGCTCCAGCCGGCCGACATCGACACCACGCAGGCACGCTGGGTTTTCTCCATCACCg                                                                                       <  1:128666/81‑1 (MQ=255)
                                                                                         acaGCGACGACCAGCAGCCTACATCGCTCCAGCCGGCCGACATCGACACCACGCAGGCACGCTGGGTTTTCTCCATCACCg                                                                                       >  2:128666/1‑81 (MQ=255)
                                                                                              gacgacCAGCAGCCTACATCGCTCCAGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCATCACCGCATAGTCAATGGAGTTGTCCGGGCAGCAGGCGAAGGTGGCTTCGTCGATGCTCAGTACa                            <  4:117713/135‑1 (MQ=255)
                                                                                              gaagacCAGAAGCCTACATCGCTCCAGCCGGCCGACATCGGCACCACGCAGGCACTCTGGGTTTTCTCCATCACCGCATAGTCAATGGAGTTGTACGGGCAGAAGGCGAATTTGGCTTCGTCGATGCTAAGTACa                            <  8:297100/132‑1 (MQ=255)
                                                                                                 gacCAGCAGCCTACATCGCTCCAGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCATCACCGCATAGTCAATGGAGTTGTCCGGGCAGCAGGCGAAGGTGGCTTCGTCGATGCTCAGTACATCg                         >  6:141850/1‑135 (MQ=255)
                                                                                                     agcagcCTACATCGCACCAGCCGCCCGACATCCGCACCACGCAGGCACGCACGGATTACTCCATCACCGCATACACAAAGGAGTTGTCCGCGCAGCAGGCCAACGTCGCTACGTCGATCCACACAACATCGCCga                     >  8:273220/1‑134 (MQ=255)
                                                                                                        agcCTACATCGCTCCAGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCATCACCGCATAGTCAATGGAGTTGTCCGGGCAGCAGGCGAAGGTGGCTTCGTCGATGCTCAGTACATCGCCGTCtt                  <  5:143046/135‑1 (MQ=255)
                                                                                                        agcCTACATCGCTCCAGCCGGCCGACATCGGCACCACGCAGGCACGCGGGGGTTTCTCCATCACCGCATAGTCAATGGAGTTGTCCGGGCAGCAGGCGAAGGTGGCTTCGTCGATGCTCAGTACATCGCCGTCtt                  <  3:284194/135‑1 (MQ=255)
                                                                                                          ccTACATCGCTCCAGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTtctc                                                                                               <  2:285554/56‑1 (MQ=255)
                                                                                                          ccTACATCGCTCCAGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTtctc                                                                                               >  1:285554/1‑56 (MQ=255)
                                                                                                            tACATCGCTCCAGCCGGCCGACATCGACACCACGCAGGCACGCTGGGTTTTCTCCATCACCGCATAGTCAATGGAGTTGTCCGGGCAGCAGGCGAAGGTGGCTTCGTCGATGCTCAGTACATCGCCGTCTTCCTg              <  7:62759/135‑1 (MQ=255)
                                                                                                               aTCGCTCCATCCGGCCGACATCGGCACCACGCAGGCACGCTGGTTTTTCTCCATCACCGCATAGTCAATGGAGTTGTCCGGGCAGCAGGCGAAGGGGGCTTCGTCGATGCTCAGTACATCGCCGTCTTCCTgg             <  8:206786/133‑1 (MQ=255)
                                                                                                               aTCGCTCCAGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCATCACCGCATAGTCAATGGAGTTGTCCGGGCAGCAGGCGAAGGTGGCTTCGTCGATGCTCAGTACATCGCCGTCTTCCTgg             <  3:298889/133‑1 (MQ=255)
                                                                                                               aTCGCTCCAGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCATCACCGCATAGTCAATGGAGTTGTCCGGGCAGCAGGCGAAGGTGGCTTCGTCGATGCTCAGTACATCGCCGTCTTCCTGGc            <  6:59394/134‑1 (MQ=255)
                                                                                                                   cTCCAGCCGGCCGACATCGGCACCACGCAGGCACGCTTGGTTTTCTCCATCACCTCATAGTCAATGGAGTTGTCCGGGCAGCAGGCGAAGGTGGCTTCGTCGATGCTCAGTACATCGCCGTCTTCCTGGCTgcg        <  2:77537/134‑1 (MQ=255)
                                                                                                                       aGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCATCACCGCATAGTCAATGGAGTTGTCCGGGCAGCAGGCGAAGGTGGCTTCGTCGATGCTCAGTACATCGCCGTCTTCCTGGCTGCGCTCCa   >  6:71701/1‑135 (MQ=255)
                                                                                                                       aGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCATCACCGCATAGTCAATGGAGTTGTCCGGGCAGCAGGCGAAGGTGGCTTCGTCGATGCTCAGTACATCGCCGTCTTCCTGGCTGCGCTCCa   >  4:159209/1‑135 (MQ=255)
                                                                                                                       aGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCATCACCGCATAGTCAATGGAGTTGTCCGGGCAGCAGGCGAAGGTGGCTTCGTCGATGCTCAGTACATCGCCGTCTTCCTGGCTGCGCTCCa   >  3:6377/1‑135 (MQ=255)
                                                                                                                       aGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCAGCACCGCATAGTCAATGGAGTTGTCCGGGCAGCAGGCGAAGGTGGCTTCGTCGATGCTCAGTACATCGCCGTCTTCCTTGCTGCGCTCCa   >  6:274147/1‑135 (MQ=255)
                                                                                                                        gccggccgACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCATCACCGCATAGTCAATGGAGTTGTCCGGGCAGCAGGCGAAGGTGGCTTCGTCGATGCTCAGTACATCGCCGTCTTCCTGGCTGCGCTCCAg  <  6:96337/135‑1 (MQ=255)
                                                                                                                        gccggccgACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCATCACCGCATAGTCAATGGAGTTGTCCGGGCAGCAGGCGAAGGTGGCTTCGTCGATGCTCAGTACATCGCCGTCTTCCTGGCTGCGCTCCAg  <  1:40720/135‑1 (MQ=255)
                                                                                                                        gccggccgACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCATCACCGCATAGTCAATGGAGTTGTCCGGGCAGCAGGCGAAGGTGGCTTCGTCGATGCTCAGTACATCGCCGTCTTCCTGGCTGCGCTCCAg  <  8:35858/135‑1 (MQ=255)
                                                                                                                        gccggccgACATCGGCACCACGCAGGCACGCAGGGTTTTCTCCCTCACCGCATAGTCAATGGAGTGGTCCGGGCAGCAGGCGAACGTGGCTTCGTCGATGCTCAGTACATCGCCGTCTTCCTGGCTGCGCTCCAg  <  2:141209/135‑1 (MQ=255)
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GTTGCCGTGGATCATGCAGTTGCGGCTGTCTTGAACCACCACGTCGCCCTTGGTGACGTTGCCGTTGTCGTCTTTCTCGTGCACTTCCCACAGCGACGACCAGCAGCCTACATCGCTCCAGCCGGCCGACATCGGCACCACGCAGGCACGCTGGGTTTTCTCCATCACCGCATAGTCAATGGAGTTGTCCGGGCAGCAGGCGAAGGTGGCTTCGTCGATGCTCAGTACATCGCCGTCTTCCTGGCTGCGCTCCAG  >  ACB122/1451728‑1451982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑