Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,630,146 G→A 11.4% E180E (GAG→GAA)  pdxJ → Pyridoxine 5'‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,630,1460GA11.4% 97.6 / 4.6 35E180E (GAG→GAA) pdxJPyridoxine 5'‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (9/22);  new base A (2/2);  total (11/24)
Fisher's exact test for biased strand distribution p-value = 5.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01

ACGCCGACGAACGGCAGATCGAGGCCTCGCGTCGGGTTGGCGCACCGGCCATCGAGCTTCACACCGGCCGTTATGCCGATGCCGAAACCCCCACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTACCACAACGTCGAGGCGGTGGCGGCGATCAAGGGTATCAATGAGTTGAACATTGGCCATGCGCTGGTGGCGCATG  >  ACB122/1630019‑1630279
                                                                                                                               |                                                                                                                                     
aCGCCGACGAACGGCAGATCGAGGCCTCGCGTCGGGTTGGCGCACCGGCCATCGAGCTTCACACCGGCCGTTATGCCGATGCCGAAACCCCCACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAGGGTGTgg                                                                                                                                <  8:45229/135‑1 (MQ=255)
       cgaACGGCAGATCGAGGCCTCGCGTCGGGTTGGCGCCCCGGCCATCGAGCTTCACACCGGCCGTTATGCCGATGCCGAAACCCCCCCCGCAGGGGCTGAAGAGCTCAAGCGGATCGTCGAGGGGGGGGCGTTTgg                                                                                                                         <  2:206998/135‑1 (MQ=255)
             gCAGATCGAGGCCTCGCGTCGGGTTGGCGCACCGGCCATCGAGCTTCACACCGGCCGTTATGCCGATGCCGAAACCCCCACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAGGGTGTGGCGTTTGGCGTggg                                                                                                                   <  4:84887/135‑1 (MQ=255)
                         cTCGCGTCGGGTTGGCGCACCGGCCATCGAGCTTCACACCGGCCGTTATGCCGATGCCGAAACCCCCACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGAt                                                                                                       >  7:272009/1‑135 (MQ=255)
                               tCGGGTTGGCGCACCGGCCATCGAGCTTCACACCGGCCGTTATGCCGATGCCGAAACCCCCACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCaa                                                                                                 <  4:103445/135‑1 (MQ=255)
                                   gTTGGCGCACCGGCCATCGAGCTTCACACCGGCCGTTATGCCGATGCCGAAACCCCCACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGcc                                                                                             <  7:161910/135‑1 (MQ=255)
                                         gcACCGGCCATCGAGCTTCACACCGGCCGTTATGCCGATGCCGAAACCCCCACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAGGGTGTGGCGTTTGGCGTGGGGCATggg                                                                                                            >  1:222663/1‑114 (MQ=255)
                                         gcACCGGCCATCGAGCTTCACACCGGCCGTTATGCCGATGCCGAAACCCCCACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAGGGGGTGGCGTTTGGCGTGGGGCATggg                                                                                                            <  2:222663/114‑1 (MQ=255)
                                          cACCGGCCATCGAGCTTCACACCGGCCGTTATGCCGATGCCGAAACCCCCACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACg                                                                                      <  8:162735/135‑1 (MQ=255)
                                           aCCGGCCATCGAGCTTCACACCGGCCGTTATGCCGATGCCGAACCCCCCACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAGGGTGTGGCGTTTGGAGTGGGGCATGGGCTGATGGTGAATGCCGGGTACgg                                                                                     >  5:84127/1‑135 (MQ=255)
                                               gCCATCGAGCTTCACACCGGCCGTTATGCCGATGCCGAAACCCCCACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCCCGGGCt                                                                                  >  4:63231/1‑134 (MQ=255)
                                                    cGAGCTTCACTGCGGCCGTTATGCCGATGACGAACCCCCCACCGAAGTGGCTGAAGAGCTCTAGCGGATCGTCGAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTa                                                                            <  4:52630/135‑1 (MQ=255)
                                                    cGAGCTTCACACCGGCCGTTATGCCGATGCCGAAACCCCCACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTa                                                                            <  3:240083/135‑1 (MQ=255)
                                                    cGAGCTTCACACCGGCCGTTATGCCGATGCCGAAACCCCCACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTa                                                                            <  3:244152/135‑1 (MQ=255)
                                                             cacCGGCCGTTAGGCCGATGCCGAAACCCCCACCGAAGTGGGTGAAGAGCTGAAGCGGATGGGAGAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTACCACAACGt                                                                   <  5:228100/135‑1 (MQ=255)
                                                                     gTTATGCCGATGCCGAAACCCCCACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTACCACAACGTCGAGGCgg                                                           >  4:216761/1‑135 (MQ=255)
                                                                     gTTATGCCGATGCCGAAACCCCCACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAAGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTACCACAACGTCGAGGCgg                                                           >  2:29732/1‑135 (MQ=255)
                                                                     gTTATGCCGATGCCGAAACCCCCACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAAGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTACCACAACGTCGAGGCgg                                                           >  2:24468/1‑135 (MQ=255)
                                                                         tgccgatgccgaAACCCCCACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAAGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTACCACAACGTCGAGGCGGTgg                                                        <  1:29732/134‑1 (MQ=255)
                                                                         tgccgatgccgaAACCCCCACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAAGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTACCACAACGTCGAGGCGGTgg                                                        <  1:24468/134‑1 (MQ=255)
                                                                               tgccgaAACCCCCACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTACCACAACGTCGAGGCGGTGGCGGCGAt                                                 <  3:216761/135‑1 (MQ=255)
                                                                                  cgaAACCCCCACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTACCACAACGTCGAGGCGGTGGCGGCGATCaa                                              <  5:103214/135‑1 (MQ=255)
                                                                                  cgaAACCCCCACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTACCACAACGTCGAGGCGGTGGCGGCGATCa                                               <  7:241117/134‑1 (MQ=255)
                                                                                         cccACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAGGGTGTGGCGTTTGGCGTGGGGGATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTACCACAACGTCGAGGCGGTGGCGGCGATCAAGGGTATc                                       <  8:263801/135‑1 (MQ=255)
                                                                                         cccACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTACCACAACGTCGAGGCGGTGGCGGCGATCAAGGGTATc                                       <  8:272009/135‑1 (MQ=255)
                                                                                              cGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTACCACAACGTCGAGGCGGTGGCGGCGATCAAGGGTATCAATGa                                  <  3:63231/135‑1 (MQ=255)
                                                                                                        gAAGAGCTCAAGCGGATCGTCGAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTACCACAACGTCGAGGCGGTGGCGGCGATCAAGGGTATCAATGAGTTGAACAtt                        >  8:232241/1‑135 (MQ=255)
                                                                                                        gAAGAGCTCAAGCGGATCGTCGAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTACCACAACGTCGAGGCGGTGGCGGCGATCAAGGGTATCAATGAGTTGAACAtt                        >  8:1081/1‑135 (MQ=255)
                                                                                                              cTCAAGCGGATCGTCGAGGGTGTGGCGGTTGGCGGGGGGCATGGGCTGATCGTCACTGCCGGGCACGGGCTGCATTACCACCACGTCGAGGCGGTGGCGGCGATCAAGGGTATCAATGAGTTGAACATTGGCCAt                  <  8:47575/135‑1 (MQ=255)
                                                                                                                        tcgtcgAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTACCACAACGTCGAGGCGGGGGCGGCGATCAAGGGTATCAATGAGTTGAACATTGGCCATGCGCtggtgg        <  5:281313/135‑1 (MQ=255)
                                                                                                                        tcggcgAGGGGGTGGCGGTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTACCACAACGTCGAGGCGGTGGCGGCGATCAAGGGTATCAATGAGTTGAACATTGGCCATGCGCtggtgg        <  1:148478/135‑1 (MQ=255)
                                                                                                                         cgtcgAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTACCACAACGTCGAGGCGGTGgcggcg                                                   >  1:284902/1‑91 (MQ=255)
                                                                                                                         cgtcgAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTACCACAACGTCGAGGCGGTGgcggcg                                                   <  2:284902/91‑1 (MQ=255)
                                                                                                                           tcgAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTACCACAACGTCGAGGCGGTGGCGGCGATCAAGGGTATCAATGAGTTGAACATTGGCCATGCGCTGGTGgcgc     >  5:121892/1‑135 (MQ=255)
                                                                                                                              aGGGTGGGGCGTTTGGCGGGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCGGCATTACCACACCGTCGAGGCGGTGGCGGCGATCAAGGGTATCAATGAGTTGAACATTGGCCATGCGCTGGTGGCGCATg  <  4:183039/135‑1 (MQ=255)
                                                                                                                               |                                                                                                                                     
ACGCCGACGAACGGCAGATCGAGGCCTCGCGTCGGGTTGGCGCACCGGCCATCGAGCTTCACACCGGCCGTTATGCCGATGCCGAAACCCCCACCGAAGTGGCTGAAGAGCTCAAGCGGATCGTCGAGGGTGTGGCGTTTGGCGTGGGGCATGGGCTGATCGTCAATGCCGGTCACGGGCTGCATTACCACAACGTCGAGGCGGTGGCGGCGATCAAGGGTATCAATGAGTTGAACATTGGCCATGCGCTGGTGGCGCATG  >  ACB122/1630019‑1630279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑