Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,832,343 C→T 6.9% D78N (GAC→AAC)  yfgD ← putative protein YfgD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,832,3430CT 6.9% 175.3 / 3.4 58D78N (GAC→AAC) yfgDputative protein YfgD
Reads supporting (aligned to +/- strand):  ref base C (33/21);  new base T (2/2);  total (35/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.28e-01

TGTAGGGCGCGCTCACGGCAGGATCTCCAGCACCTTTTCCGGCGGGCGACCGACCACGGCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAGCAGGGCCTTGAGGGTGTCCGCGTCGGGCGGGGTTTCCAGGTAGC  >  ACB122/1832209‑1832477
                                                                                                                                      |                                                                                                                                      
tGTAGGGCGCGCTCACCGCAGGATCTCCCGCACCGTTTCCGGCGGGCGACCGACCACGGCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTCGGCCATGGCCTCGATCAGTTGCGCGTc                                                                                                                                        >  7:57059/1‑135 (MQ=255)
     ggCGCGCTCACGGCAGGATCTCCAGCACCTTTTCCGGCGGGCGACCGACCACGGCCTTGTCGCCGGCACCCAGGATCGGCCGGTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCa                                                                                                                                   >  3:285420/1‑135 (MQ=255)
     ggCGCGCTCACGGCAGGATCTCCAGCACCTTTTCCGGCGGGCGACCGACCACGGCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCa                                                                                                                                   >  4:77778/1‑135 (MQ=255)
     ggCGCGCTCACGGCAGGATCTCCAGCACCTTTTCCGGCGGGCGACCGACCACGGCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCa                                                                                                                                   >  6:7190/1‑135 (MQ=255)
     ggCGCGCTCACGGCAGGATCTCCAGCACCTTTTCCGGCGGGCGACCGACCACGGCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGGCGGtaa                                                                                                                                   >  1:114373/1‑133 (MQ=255)
        gcgcTCACGGCAGGATCTCCAGCACCTTTTCCGGCGGGCGACCGACCACGGCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGTCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTg                                                                                                                                >  8:231259/1‑135 (MQ=255)
           cTCACGGCAGGATCTCCAGCACCTTTTCCGGCTGGCGACCGACCACGGCCTGGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGGCGGTCAGTGCCg                                                                                                                             <  6:15613/135‑1 (MQ=255)
           cTCACGGCAGGATCTCCAGCACCTTTTCCGGCGGGCGACCGACCACGGCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGGTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCg                                                                                                                             >  4:174468/1‑135 (MQ=255)
           cTCACGGCAGGATCTCCAGCACCTTTTCCGGCGGGCGACCCACCACGGCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCg                                                                                                                             >  8:225524/1‑135 (MQ=255)
            tCACGGCAGGATCTCCAGCACCTTTTCCGGCGGGCGACCGACCACGGCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCgg                                                                                                                            >  5:156721/1‑135 (MQ=255)
            tCACGGCAGGATCTCCAGCACCTTTTCCGGCGGGCGACCGACCACGGCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTGGCGCGTCGGTCAGTGCCgg                                                                                                                            >  4:175774/1‑135 (MQ=255)
            tCACGGCAGGATCTCCAGCACCTTTTCCGGCGGGCGACCGACCACGGCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTATGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCgg                                                                                                                            >  6:86011/1‑135 (MQ=255)
                       tctcCAGCACCTTTTCCGGCGGGCGACCGACCACGGCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGGGCCGGGTCGGCCAGGt                                                                                                                 >  6:43342/1‑135 (MQ=255)
                         tcCAGCACCTTTTCCGGCGGGCGACCGACCACGGCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCg                                                                                                                        <  3:106695/126‑1 (MQ=255)
                         tcCAGCACCTTTTCCGGCGGGCGACCGACCACGGCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCg                                                                                                                        >  4:106695/1‑126 (MQ=255)
                            aGCACCTTTTCCGGCGGGCGACCGACCACGGCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGtt                                                                                                                <  3:86730/131‑1 (MQ=255)
                            aGCACCTTTTCCGGCGGGCGACCGACCACGGCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGtt                                                                                                                <  8:96997/131‑1 (MQ=255)
                            aGCACCTTTTCCGGCGGGCGACCGACCACGGCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGt                                                                                                                 <  4:111707/130‑1 (MQ=255)
                            aGCACCTTTTCCCGCGGGCGACCGACCACGGCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGTGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGtt                                                                                                                <  8:180434/131‑1 (MQ=255)
                                      ccGGCGGGCGACCGACCACGGCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATAtt                                                                                                  >  7:302626/1‑135 (MQ=255)
                                      ccGGCGGGCGACCGACCACGGCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATAtt                                                                                                  >  2:225072/1‑135 (MQ=255)
                                              cgaccgacCACGGCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTcg                                                                                          >  5:115861/1‑135 (MQ=255)
                                                         ggCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGCTCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCgcag                                                                               >  8:187027/1‑135 (MQ=255)
                                                                tCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGGCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATAGTCGTCCTCGCCGCTGCGCAGCAACTg                                                                         <  6:205900/134‑1 (MQ=255)
                                                                 cGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGCTCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCGTTATATTCGTCCTCGCCGCTGCGCAGCCACTGAc                                                                       >  5:20778/1‑135 (MQ=255)
                                                                 cGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGa                                                                        <  3:77778/134‑1 (MQ=255)
                                                                 cGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGGTCTGCGGTTTATATTGGTCCTCGCCGCTGCGCAGCAACTGAc                                                                       >  2:120940/1‑135 (MQ=255)
                                                                 cGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGGGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTg                                                                         <  1:86156/133‑1 (MQ=255)
                                                                 cGCCGGCAACCAGGATAGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTAGATTCGTCCTCGCCGCTGCGCAGCAACTGAc                                                                       >  6:161467/1‑135 (MQ=255)
                                                                   ccGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGAcgc                                                                     >  3:251285/1‑135 (MQ=255)
                                                                        aaCCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCgggg                                                                 >  2:22883/1‑134 (MQ=255)
                                                                                       ttCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGcc                                                 <  3:50086/135‑1 (MQ=255)
                                                                                        tCGATCAGTTTGGGGTGCTTGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCg                                                <  1:22883/135‑1 (MQ=255)
                                                                                         cGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGa                                               <  5:159783/135‑1 (MQ=255)
                                                                                         cGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGa                                               >  3:65895/1‑135 (MQ=255)
                                                                                          gATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGa                                               <  6:156721/134‑1 (MQ=255)
                                                                                          gATCAGGTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAg                                              <  5:177442/135‑1 (MQ=255)
                                                                                           aTCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAg                                              <  6:115861/134‑1 (MQ=255)
                                                                                                        gCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGACTCTGATCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAGCAGGGCCTGGAggg                                >  2:226626/1‑135 (MQ=255)
                                                                                                           gggCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAGCAGGGCCTTGAGGgtgt                             >  5:259642/1‑135 (MQ=255)
                                                                                                            ggCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAGCAGGGCCTTGAGGGTGTc                            >  6:116699/1‑135 (MQ=255)
                                                                                                            ggCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGATTTATATTCGTCATCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAGCAGGGCCTTGAGGGTGTc                            >  2:78696/1‑135 (MQ=255)
                                                                                                             gCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGGCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGCCGCGGGGCGATGCCCAGCTTGCCGAGCAGGGCCTTGAGGGTGTcc                           >  4:101831/1‑135 (MQ=255)
                                                                                                             gCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAGCAGGGCCTTGAGGGTGTcc                           >  8:76820/1‑135 (MQ=255)
                                                                                                                    ccTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAGCAGGGCCTTGAGGGTGTCCGCGTcgg                    >  3:256509/1‑135 (MQ=255)
                                                                                                                    ccTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAGCAGGGCCTTGAGGGTGTCCGCGTcgg                    >  8:215773/1‑135 (MQ=255)
                                                                                                                     cTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAGCAGGGCCTTGAGGGTGTCCGCGTcggg                   <  8:187535/135‑1 (MQ=255)
                                                                                                                     cTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGGTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAGCAGGGCCTTGAGGGTGTCCGCGTcggg                   <  2:263485/135‑1 (MQ=255)
                                                                                                                     cTCGATCAGGTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAGCAGGGCCTTGAGGGTGTCCGCGTcggg                   <  2:240272/135‑1 (MQ=255)
                                                                                                                           cAGTTGCGCGTTGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAg                                              <  5:80350/102‑1 (MQ=255)
                                                                                                                           cAGTTGCGCGTTGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAg                                              >  6:76020/1‑102 (MQ=255)
                                                                                                                           cAGTTGCGCGTTGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAg                                              >  6:80350/1‑102 (MQ=255)
                                                                                                                           cAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAGCAGGGCCTTGAGGGTGTCCGCGTCGGGCGGGGt             >  3:45844/1‑135 (MQ=255)
                                                                                                                           cAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAGCAGGGCCTTGAGGGTGTCCGCGTCGGGCGGGGt             >  1:161087/1‑135 (MQ=255)
                                                                                                                           cAGTTGCGCGGTGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAg                                              <  5:76020/102‑1 (MQ=255)
                                                                                                                             gTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCAATGCCCAGCTTGCCGAGCAGGGCCTTGAGGGTGTCCGCGTCGGGCGGGGtt            <  4:265197/134‑1 (MQ=255)
                                                                                                                               tGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAGCAGGGCCTTGAGGGTGTCCGCGTCGGGCGGGGTTTcc         <  3:219906/135‑1 (MQ=255)
                                                                                                                               tGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAGCAGGGCCTTGAGGGTGTCCGCGTCGGGCGGGGTTTcc         <  8:70318/135‑1 (MQ=255)
                                                                                                                                 cgcgTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAGCAGGGCCTTGAGGGTGTCCGCGTCGGGCGGGGTTTCCAg       >  6:293749/1‑135 (MQ=255)
                                                                                                                                      cGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAGCAGGGCCTTGAGGGTGTCCGCGTCGGGCGGGGTTTCCAGGTAgc  >  2:48915/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                      
TGTAGGGCGCGCTCACGGCAGGATCTCCAGCACCTTTTCCGGCGGGCGACCGACCACGGCCTTGTCGCCGGCAACCAGGATCGGCCGTTCGATCAGTTTGGGGTGCTGGGCCATGGCCTCGATCAGTTGCGCGTCGGTCAGTGCCGGGTCGGCCAGGTTCAGCGCTTTATATTCGTCCTCGCCGCTGCGCAGCAACTGACGCGGGGCGATGCCCAGCTTGCCGAGCAGGGCCTTGAGGGTGTCCGCGTCGGGCGGGGTTTCCAGGTAGC  >  ACB122/1832209‑1832477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑