| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 1,892,414 | A→C | 26.7% | intergenic (+428/‑92) | PP_RS08755 → / → napA | tRNA‑Asp/Periplasmic nitrate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,892,414 | 0 | A | C | 26.7% | 28.3 / 2.7 | 34 | intergenic (+428/‑92) | PP_RS08755/napA | tRNA‑Asp/Periplasmic nitrate reductase |
| Reads supporting (aligned to +/- strand): ref base A (15/9); new base C (7/3); total (22/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGCCGCCCTCAGGCCTTGCGATATCCCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGTCGACAGCCCTCACCACTTCACGACAAAGGCGCCGTGTTGCCCCGCTTGCATCAGCATGCCGGGGCAGCCGGCGCC‑TTTTTGCGTTTTCCACAGGAGCCCGCCCATGGCCAACAGCGAAGTACGCAGCGTCTGCCCCTACTGCG > ACB122/1892293‑1892545 | gcgcCGCCCTCAGGCCTTGCGATATCCCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCAGAAATTGCTGATCTACCTCATCCCGGGCTGGCAACGTCGCCAGCCCTCACCCCGTCACGAc > 1:96840/1‑134 (MQ=39) cgccCTCAGGCCTTGCGATATCCCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCGGGGAACGGGGCCCGCCCTCCCCCCGTCACGACAAAgg > 7:54806/1‑135 (MQ=37) cccTCAGGCCTTGCGATATCCCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGCTCTACCTCATCCCGGGGTGTCCACGTCGCCAGCCCGCACCACTTCACGACAAAAgcc > 1:23367/1‑134 (MQ=39) tCAGGCCTTGCGATATCCCTGTGGGAGCGGGCCTGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCGGATCTACCTCCTACCGGGCGGTCCACGTCGACAGCCCGCCCCACTGCACGACAAAGGCGccc > 8:170251/1‑134 (MQ=255) gATATCCCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACATCATCCCGGGGTGTCAACCTCGAACGCCCTCACCACGTCAAGACAAAAGCGCCGTGTTTCCCCGc > 8:24161/1‑135 (MQ=38) ccTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTGAACGTCGCCAGCCCTCACCCCTTCACCACAAAGGCGCCGGGTTGCCCCCCTTGCa > 4:299332/1‑134 (MQ=39) ccTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCGGGCAACGTCGACCGCCCTCACCACTTCACGACAAAAGCCCCGGGTTTGCCCGCTTgga > 7:178496/1‑132 (MQ=38) gAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTCGACGTCGACAGCCCACCCCACATCACCACAAAGGCGCCCTGTTGCCCCGCTTGGATCCGCCTg > 7:169766/1‑135 (MQ=255) gAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGGGAACGTCGCCCGCCCGCACCCCTTCACGACAAAGGCGCCGTGTTGCCCCGCGTGGCTGAGCAtt > 6:99419/1‑134 (MQ=255) ggCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCGGTGAACGTCGACAGCCCTCACCACTTCACGACAAAGGCGCCGTGTTGCCCCGCTTGGATCAGCATGCCggg > 5:120424/1‑135 (MQ=255) gCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGTCGACAGCCCTCACCACTTCACGACAAAGGCGCCGTGTTGCCCCGCTTGCATCAGCATGCCgggg > 7:143551/1‑135 (MQ=255) gCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACATCATCCCGGGCTGTCAACGTCGACAGCCCGCCCCACTAAAAGACAAAGGCGCCGTGTTTGCCCGCTTGGTTAAGCATGCCcggg > 6:88600/1‑135 (MQ=255) aTGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGGTGTGAACGTCGCCCGCCCGCACCCCTTCACGACAAAGGCGCCGGGTTGCCCCCGGTGGATCACCATGCCGGGGcc > 2:284461/1‑134 (MQ=255) aTGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGTCGACAGCCCTCACCACATCACGACAAAGGCGCCGTGTTTCCCCCCGTGCATCAGCAAGCCGGGGCa > 2:33071/1‑135 (MQ=255) aaGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTAAACCTCGACAGCCCGCCCCACATCACGACAAACGCGCCGTGTTGCCCCGCTTGCATCAGCAAGCCGGGGCAGCCGGGGccg > 3:20599/1‑134 (MQ=255) gCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGGCAACGTCGACAGCCCTCACCACTTCACGACAAAGGCGCCGTGTTGCCCCGCTGGCATCAGCATGCCGGGGCAGCCGGCGCC‑ttttt < 8:220395/135‑1 (MQ=255) taGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGTCGCCAGCCCTCCCCCCTTCACGCCAAAGCCGCCGTGTTTCCCCGCTTGGCTCCTCATGCCGGGGCCGCCGGCGCC‑GTTTTTCGgtt > 6:8021/1‑134 (MQ=255) gCCCATAACTTGCCGAGCTCCCTCACCCCGGGCGGGCAACGTCGACCGCCCTCCCCACTTCACGACAAAGGCGCCGTGGTGCCCCGCTGGCATCAGCATGCGGGGGCAGCCGGCGCC‑TTTTTGCGTTTTCCACAg < 7:292585/135‑1 (MQ=255) gCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGTCGCCAGCCCTCACCACTTCACGACAAAGGCGCCGTGTTTCCCCGCGTGCATCAGCATGCCGGGGCAGCCGGCGCC‑GTTTTTCGGTTTGCACAg > 1:172512/1‑135 (MQ=255) aTAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGTCGCCAGCCCTCACCACTTCACGACAAAGGCGCCGTGTTGCCCCGCTTGCATCAGCATGCCGGGGCCGCCCGCGCCGTTTTTGGGTTTT‑CACAGgcgc > 6:86012/1‑135 (MQ=255) aTAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGTCGACAGCCCTCACCACTTCACGACAAAGGCGCCGTGTTGCCCCGCTTGCATAAGCATGCCGGGGCAGCCGGCGCC‑GTTTTGCGGTTTGCCCAGgcgc > 8:89189/1‑135 (MQ=255) aTAAATTGCTGATCTACATCATCCCGGGCTGTAAACGTCGACAGCCCTGACCACTTCACCACAAAGGCGCAGGGTTGCCACCCATGGATCAACATGCCGGGGCACCCGGCCGC‑ATTTTTCCTTTTACACCGGAgc > 2:158343/1‑135 (MQ=255) tAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGTCGACAGCCCTCCCCACTTCACGACAAACGCGCCGTGTTGCCCCGCTTGCATCAGCATGCCGGGGCCGCCGGCGCC‑CTTTTGCGTTTTACACACGCgcc > 8:150911/1‑135 (MQ=255) tGATCTACCTCACCCCGGGCTGTCAACGTCGACCGCCCTCACCACTTCACCACAAAGGCGCGGTGTTGCCCCGCTTGCATCAGCATGCCGGGGCAGCCGGCGCC‑TTTTTGCGTTTTCCACAGGAGCCCGCCCAt < 7:150911/134‑1 (MQ=255) tCTACCTCATCCCGGGCTGTCAACGTCGACAGCCCTCCCCCCTTCACGAAAACGGCGCGGTGTGGCCCCGCTTGCATCTGCATGCGGGGGCAGCGGGCGCC‑TTTTTGCGTTTTCCACAGGAGCCCGCCCATGGc < 7:24161/134‑1 (MQ=255) ccGGGCTGTCAACGTCGACAGCCCTCACCACTTCACGACAACGGCGCCGTGTTGCCCCGCTTGCATCAGCAGGCCGGGGCAGCCGGCGCC‑TTTTTGCGTTTTCCACAGGAGCCCGCCCATGGCCAACAGCGAAGt < 7:20102/135‑1 (MQ=255) gacaccGTCGAAAGCCCTCACCACTTAACGAAAATGGCGGCTTGTTGCCCCGCTTGCATCAGCTTGGGGGGGCAGCGGGGGCT‑TTTTTGCGTTTTCCACTGGAGCCCGCCCATGGCAAACAGCTAAGTACGCAGc < 6:96447/130‑1 (MQ=255) cAACGGCGACAGCCCTCACCACTTCACGACAAAGGCGCCGTGTTGCCCCGCTTGCAGCAGCATGCCGGGGCAGCCGGCGCC‑TTTTTGCGTTTTCCACAGGAGCCCGCCCATGGCCAACAGCGAAGTACGCAGc < 8:159898/133‑1 (MQ=255) aaCGTCGACAGCCCTCACCACTTCACGACAACGGCGCCGTGTTGCCCCGCTGGCATCAGCATGCCGGGGCAGCCGGCGCC‑TTTTTGCGTTTTCCACAGGAGCCCGCCCATGGCCAACAGCGAAGTACGCAGCGTc < 1:3873/135‑1 (MQ=255) aCGTCGACAGCCCTCACCACTTCACGACAAAGGCGCCGTGTTGCCCCGc > 7:271381/1‑49 (MQ=255) aCGTCGACAGCCCTCACCACTTCACGACAAAGGCGCCGTGTTGCCCCGc < 8:271381/49‑1 (MQ=255) ggcGACAGCCCTCACCACTTCACGACAAAGGCGCCGTGTGGCCCCGCTTGCATCAGCATGCCGGGGCAGCCGGCGCC‑TTTTTGCGTTTTCCACAGGAGCCCGCCCATGGCCAACAGCGAAGTACGCAGCGTCTGc < 5:86012/133‑1 (MQ=255) ggACCGCCCTCCCCACTTCACCCCAAGGGCCCCGGGTTGCCCCGCTTGCATCAGCATGGGGGGGCAGCGGGCGCC‑TTTTTGCGAGTTCCACAGGAGCCCGCCCATGGCCAACAGCGAAGTACGCAGCGTCTGccc < 3:117793/134‑1 (MQ=255) cGACCGCCCCCACCAATCCCCGACAACGGCGCCGTGTTGCCCCGCTGGCTGACGCAGGCGGGGTCAGCCGGCGCC‑TTTTTGCGTTTTCCACAGGAGCCCGCCCATGGCCAACAGCGAAGTACGCAGCGTCTGccc < 3:202474/135‑1 (MQ=255) cgcaccccTTCCCGACAACGGCGCCGGGTTCCCCCGATTGCAGCAGCATGCCGGGGCAGCGGGGGCC‑TTTTTGCGTTTTCCACAGGAGCCCGCCCATGGCCAACAGCGAAGTACGCAGCGTCTGCCCCTACTgcg < 7:72615/133‑1 (MQ=255) | GCGCCGCCCTCAGGCCTTGCGATATCCCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGTATAGCTGGCCCATAAATTGCTGATCTACCTCATCCCGGGCTGTCAACGTCGACAGCCCTCACCACTTCACGACAAAGGCGCCGTGTTGCCCCGCTTGCATCAGCATGCCGGGGCAGCCGGCGCC‑TTTTTGCGTTTTCCACAGGAGCCCGCCCATGGCCAACAGCGAAGTACGCAGCGTCTGCCCCTACTGCG > ACB122/1892293‑1892545 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |