Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 65,165 G→A 6.0% N31N (AAC→AAT)  CJ019_00068 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB12265,1650GA 6.0% 225.2 / 2.1 68N31N (AAC→AAT) CJ019_00068hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (36/27);  new base A (2/2);  total (39/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTGCAGCTGATAGGCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTAGAAGAAACTCAAGCCGACGTGGAACAGC  >  ACB122/65031‑65299
                                                                                                                                      |                                                                                                                                      
ccGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTGCAGCTGATAGGCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCg                                                                                                                                        >  2:121482/1‑135 (MQ=255)
             cTTTGCGCGCCGCCGCCAGCTCGCCCTGCAGCTGATAGGCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATcca                                                                                                                           <  8:90606/135‑1 (MQ=255)
             cTTTGCGCGCCGCCGCCAGCTCGCCCTGCAGCTGATAGGCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATcca                                                                                                                           <  4:261423/135‑1 (MQ=255)
                       cgccgccAGCTCGCCCTGCAGCTGATAGGCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGTTCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCtt                                                                                                                 <  1:87903/135‑1 (MQ=255)
                              aGCTCGCCCTGCAGCTGATAGGCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAgg                                                                                                          <  7:280680/135‑1 (MQ=255)
                              aGCTCGCCCTGCAGCTGATAGGCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAgg                                                                                                          <  6:64609/135‑1 (MQ=255)
                                 tCGCCCTGCAGCTGATAGGCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGTACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCa                                                                                                       <  5:254927/135‑1 (MQ=255)
                                          aGCTGATAGGCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTca                                                                                                <  3:274656/133‑1 (MQ=255)
                                          aGCTGATAGGCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTca                                                                                                <  8:203984/133‑1 (MQ=255)
                                          aGCTGATAGGCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTca                                                                                                <  6:244384/133‑1 (MQ=255)
                                             tGATAGGCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCagcagc                                                                                           <  3:14819/135‑1 (MQ=255)
                                                  ggCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCCGCCAGGCCTTGCCCAGGCCAAACCTCCGCAGCGGGtt                                                                                      >  5:144531/1‑135 (MQ=255)
                                                  ggCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCTAACCTca                                                                                                <  5:179954/125‑1 (MQ=255)
                                                  ggCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTca                                                                                                >  6:179954/1‑125 (MQ=255)
                                                       tACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCca                                                                                 >  2:51008/1‑135 (MQ=255)
                                                         cacagcagCTTCCATGCTGCCGACTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCAGTTGGCTTCATCAAGACAGTCCTTGCCCATGCAATACCTCAGCAGAGGGTTATGcaaa                                                                               >  5:19023/3‑133 (MQ=255)
                                                          ccAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTCCCTTCCTCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCcacaa                                                                              >  2:18811/1‑134 (MQ=255)
                                                           cagcagCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCAcccc                                                                             >  7:255445/1‑135 (MQ=255)
                                                           cagcagCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACAcc                                                                             >  8:3767/1‑135 (MQ=255)
                                                                gcTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTgg                                                                        <  1:121482/135‑1 (MQ=255)
                                                                 cTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTgg                                                                        >  7:250189/1‑134 (MQ=255)
                                                                 cTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGc                                                                       >  1:115957/1‑135 (MQ=255)
                                                                  ttCCATGCTGCCGAGTATTTCGGATCCTGCTATACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGcc                                                                      <  3:277323/135‑1 (MQ=255)
                                                                      aTGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCGTCATCCAGCCAGGCCTTGCCCAGGCCACACCTCAGCAGCGGGTTATCCACCCCTTTGGCCAGCa                                                                  >  5:133215/1‑135 (MQ=255)
                                                                              gAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCAtcttct                                                            >  4:43679/1‑133 (MQ=255)
                                                                                    ttCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGAt                                                    >  5:72495/1‑135 (MQ=255)
                                                                                         aTCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTcgc                                                >  8:36599/1‑134 (MQ=255)
                                                                                          tCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCgcgc                                              <  2:31020/135‑1 (MQ=255)
                                                                                               agctACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGct                                         <  1:292500/133‑1 (MQ=255)
                                                                                                tctACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGctc                                        >  4:37092/1‑135 (MQ=255)
                                                                                                       aGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTtctc                                 >  1:199287/1‑135 (MQ=255)
                                                                                                           tGCCAGGTGCACCGCTGCTTCAGCGCCATTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTag                             <  1:192488/135‑1 (MQ=255)
                                                                                                           tGCCAGGTGCACCGCTGCTTCAGCGCCATTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTag                             <  1:188190/135‑1 (MQ=255)
                                                                                                            gCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATcca                                                                                                                           <  6:195794/40‑1 (MQ=255)
                                                                                                            gCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATcca                                                                                                                           >  5:195794/1‑40 (MQ=255)
                                                                                                            gCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCa                                                                                                            >  6:131439/1‑55 (MQ=255)
                                                                                                            gCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCa                                                                                                            >  6:255737/1‑55 (MQ=255)
                                                                                                            gCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCa                                                                                                            <  5:255737/55‑1 (MQ=255)
                                                                                                            gCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCa                                                                                                            <  5:131439/55‑1 (MQ=255)
                                                                                                            gCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAgg                                                                                                          >  2:74079/1‑57 (MQ=255)
                                                                                                            gCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAgg                                                                                                          <  1:74079/57‑1 (MQ=255)
                                                                                                            gCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTca                                                                                                <  8:177170/67‑1 (MQ=255)
                                                                                                            gCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTca                                                                                                >  7:177170/1‑67 (MQ=255)
                                                                                                            gCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGtt                                                                                      >  3:38318/1‑77 (MQ=255)
                                                                                                            gCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGtt                                                                                      <  4:38318/77‑1 (MQ=255)
                                                                                                            gCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTa                              >  3:165786/1‑133 (MQ=255)
                                                                                                            gCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTa                              >  3:275411/1‑133 (MQ=255)
                                                                                                            gCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTa                              >  7:2161/1‑133 (MQ=255)
                                                                                                            gCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTa                              >  2:168017/1‑133 (MQ=255)
                                                                                                            gCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTa                              >  5:128057/1‑133 (MQ=255)
                                                                                                            gCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTa                              >  5:61687/1‑133 (MQ=255)
                                                                                                            gCCAGGTGCACCGCTGCTTCAGCGCCCTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTATGCCCAGCATCTTCTCCAGCGATTCGCGCATTCTCTTTTCTCCTa                              >  8:80673/1‑133 (MQ=255)
                                                                                                            gCCAGGTGCACCGCTGCTTCAGCGCCATTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTa                              >  2:192488/1‑133 (MQ=255)
                                                                                                            gCCAGGTGCACCGCTGCTTCAGCGCCATTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTa                              >  2:188190/1‑133 (MQ=255)
                                                                                                               aGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCCGGCCAAACCTCAGCAGCGGGTGATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTagaag                          >  4:26522/1‑134 (MQ=255)
                                                                                                               aGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTagaat                          >  8:103230/1‑133 (MQ=255)
                                                                                                               aGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTagaag                          >  8:191897/1‑134 (MQ=255)
                                                                                                               aGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTagaag                          >  7:104920/1‑134 (MQ=255)
                                                                                                               aGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCGTCTCCCGCGATTCGCGCCTGCTGGTTTGTCCTagaact                         >  2:292500/1‑133 (MQ=255)
                                                                                                                      ccGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCCCACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTAGAAGAAACTCaa                  >  2:33821/1‑135 (MQ=255)
                                                                                                                            ctTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTAgaagaa                        <  7:193854/123‑1 (MQ=255)
                                                                                                                            ctTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTAgaagaa                        >  8:193854/1‑123 (MQ=255)
                                                                                                                             ttCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTAGAAGAAACTCAAGCCGa             <  3:212643/133‑1 (MQ=255)
                                                                                                                              tCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTAGAAGAAACTCAAGCCGACGt          <  2:115957/135‑1 (MQ=255)
                                                                                                                               cAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTAGAAGAAACTCAAGCCGACGt          >  3:91249/1‑134 (MQ=255)
                                                                                                                                 gcgcCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTAGAAGAAACTCAAGCCGACGTgg        <  6:288764/134‑1 (MQ=255)
                                                                                                                                 gcgcCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTAGAAGAAACTCAAGCCGACGTgg        <  2:199287/134‑1 (MQ=255)
                                                                                                                                  cgcCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTAGAAGAAACTCAAGCCGACGTGGaa      <  7:3767/135‑1 (MQ=255)
                                                                                                                                    ccGTTGCCGTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACCCCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCGTTTCTCCGAGAAGAAACTCAAGCCGACGAGGAAcc    >  6:91247/1‑134 (MQ=255)
                                                                                                                                      gTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTAGAAGAAACTCAAGCCGACGTGGAACAGc  <  3:202837/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
CCGTCTTCCCACGCTTTGCGCGCCGCCGCCAGCTCGCCCTGCAGCTGATAGGCCTTACCCAGCAGCTTCCATGCTGCCGAGTATTTCGGATCCTGCTCTACACAGCGTGCCAGGTGCACCGCTGCTTCAGCGCCGTTGCCTTCATCCAGCCAGGCCTTGCCCAGGCCAAACCTCAGCAGCGGGTTATCCACACCTTTGGCCAGCATCTTCTCCAGCGATTCGCGCATGCTCTTTTCTCCTAGAAGAAACTCAAGCCGACGTGGAACAGC  >  ACB122/65031‑65299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑