| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 2,391,010 | T→G | 48.9% | E566D (GAA→GAC) | bvgS ← | Virulence sensor protein BvgS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 2,391,010 | 0 | T | G | 48.9% | ‑1.8 / 2.7 | 16 | E566D (GAA→GAC) | bvgS | Virulence sensor protein BvgS |
| Reads supporting (aligned to +/- strand): ref base T (2/6); new base G (6/2); total (8/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCACGGCTTCAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATCTGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGCCAGGCGGCTGTAGCCT > ACB122/2390885‑2391124 | tGCACGGCTTCAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCATCGCGCGGTGGgcgcgc > 4:58233/1‑135 (MQ=255) cAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTGAGCCCGCCTTCCGCCCcctggcgcgggc > 6:16314/1‑135 (MQ=255) cAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGGTCGGCGCgctggcgctggc > 4:272494/1‑135 (MQ=255) aGCTGTAGTTGCAACTTTTCAGGCACCCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTGCAGCGCGCGGTCGGCGCGCGGGCGCTGGCGAATc < 2:6971/134‑1 (MQ=255) tAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAACGCGCGGTTGGCGCGCTGGGGGAGGGGCATCTggagg > 6:68956/1‑135 (MQ=255) aaCTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTGCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATCTgcaggcgcaggc < 7:233690/135‑1 (MQ=255) gccaCCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATCTGCAGGCGCAGGCGGGCATTCCa < 2:46394/132‑1 (MQ=255) gcgGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATCTGCAGGCGCAGGCGGGCATTCCAGAGCAGGGc < 6:116990/135‑1 (MQ=255) tCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGGGTGCGGCGCGCTGGCGCTGGCGAATCTGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGc < 7:281403/135‑1 (MQ=255) acatacatGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTGACCGCCCGTTAGGGGCCCTGGCGCGGGCGCAACTGCACGCCGCGGCGGGCCTGACACACCACGGGCCGCAACaaa > 8:100144/1‑135 (MQ=255) gggggggCGTACCGGTGAGCAGGGCGCGCAGGAATGCCAACTGGGCGTTCCGCGCGCGTGCGGCGCGCTGGCGCTGGCGAAGCTGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCg < 4:195021/132‑1 (MQ=255) ggCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTAAGCGCGCGGTCGGCGCGCTGGCGCTGGCGAATCTGCAGGCGCAGGCGGGCCTTCCAGAGCCGGGCCAGCAACAAAAGCACGGCCGTGGcc > 1:209709/1‑135 (MQ=255) gTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTGAGCCCGCGGTCGGCGCGCGGGCGGGGGCGAATCTGCAGGGGGCGGCGGGGCGTCCAGCGCAGGGCCACGCACAAAATCACGGCCGGGGCCCGcacccc > 7:296993/1‑133 (MQ=255) gcgcATGAATGCCAACTGGTCGTTAAGCGCGCGGTCGGCGCGCGGGCGCTGGCGAATCTGCAGGCGCAGGCGGGGCTTGCAGAGCCGGGGCCGCAAAAAAAGCACGGCCGTGGGCACCCCCCCCAGCAGGGCCAg > 4:255071/1‑135 (MQ=255) cttGACTGCCAACGGGGCGTCCCGCGCGGGGGCGGCGCGCGGGCGCGGGCGAATCTGCCGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGAAACAAACCCACGGCCGTGGCCAGCACCACCTGCAGGGCCAggcg < 1:85745/133‑1 (MQ=255) aaCTGGTCGTTCAGCGCGCGTGCGGCGCGCTGGCGCTGGCGAATCTGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGCCAGGCGGCTGTAGCCt < 4:71834/135‑1 (MQ=255) | TGCACGGCTTCAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATCTGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGCCAGGCGGCTGTAGCCT > ACB122/2390885‑2391124 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |