Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 2,503,428 C→T 7.3% G454S (GGT→AGT)  copA_2 ← Copper resistance protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,503,4280CT 7.3% 175.2 / 4.1 55G454S (GGT→AGT) copA_2Copper resistance protein A
Reads supporting (aligned to +/- strand):  ref base C (23/28);  new base T (2/2);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

GGTGATGCGCACGCGCTCACCGTAGGTCAGCCGCAGCGGTTCGGCGTCGCTGAACTTGATGCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCAGGATCGGCCAGGTTGGGGCGCGGTGCCATGGTCTGCATGTCCA  >  ACB122/2503294‑2503547
                                                                                                                                      |                                                                                                                       
ggTGATGCGCACGCGCTCACCGTAGGTCAGCCGCAGCGGTGCGGCGTCGCTGAACTTGATGCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGAcc                                                                                                                         <  4:200866/135‑1 (MQ=255)
  tGATGCGCACGCGCTCACCGTAGGTCAGCCGCAGCGGTTCGGCGTCGCTGAACTTGCTGCCGTCGAACGACCAGGCGAACCGCTCCATATGGCCGGGCAGGTGGAGCTCGATGTCTCGCGCTGGCGGGCGCCCGt                                                                                                                       >  5:81899/1‑135 (MQ=255)
  tGATGCGCACGCGCTCACCGTAGGTCAGCCGCAGCGGTTCGGCGTCGCTGAACTTGATGCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGt                                                                                                                       >  7:84723/1‑135 (MQ=255)
   gATGCGCACGCGCTCACCGTAGGTCAGCCGCAGCGGTTCGGCGTCGCTGAACTTGCTGCCGTCGAACGCCCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCCGCTCGATGTCACGCGATGGCGGGCGGCCggc                                                                                                                      >  5:178239/1‑133 (MQ=255)
   gATGCGCACGCGCTCACCGTAGGTCAGCCGCAGCGGTTCGGCGTCGCTGAACTTGATGCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTc                                                                                                                      >  8:281077/1‑135 (MQ=255)
      gcgcACGCGCTCACCGTAGGTCAGCGGCAGCGGTTCGGCGTCGCTGAACTTGATGCCGGCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCgg                                                                                                                   <  2:110130/135‑1 (MQ=255)
      gcgcACGCGCTCACCGTAGGTCAGCCGCAGCGGTTCGGCGTCGCTGAACTTGATGCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCgg                                                                                                                   <  7:281077/135‑1 (MQ=255)
      gcgcACGCGCTCACCGTAGGTCAGCCGCAGCGGTTCGGCGTCGCTGAACTTGATGCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCgg                                                                                                                   <  4:44391/135‑1 (MQ=255)
       cgcACGCGCTCACCGTAGGTCAGCCGCAGCGGTTCGGCGTCGCTGAACTTGATGCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCgg                                                                                                                   <  8:84723/134‑1 (MQ=255)
        gcACGCGCTCCCCGTAGGTCAGCCGCAGCGGTTCGGCGTCGCTGAACTTGATGCCGTAGAACGACCAGGCGAAGCGCGCCATATGGCAGGTCAGGTGCAGCTCGATGTGACGCGAAGGCGGGCGACCCTCCGGGt                                                                                                                 >  6:38084/1‑135 (MQ=255)
        gcACGCGCTCACCGTAGGTCAGCCGCAGCGGTTCGGCGTCGCTGCACGTGATGCCGTCGAACGACCAGGCGAAGCGCGCCATATGGCCGGTCAGGTGCAGCTCGCTGTCACGCGATGGCGGGCGCCCGTCCgggg                                                                                                                 >  6:178904/1‑134 (MQ=255)
        gcACGCGCTCACCGTAGGGCAGCCGCAGCGGGTCGGCGTCGCTGAACTTGATGCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCgg                                                                                                                   <  5:226809/133‑1 (MQ=255)
             cgcTCACCGTAGGTCAGCCGCAGCGGTTCGGCGTCGCTGAACTTGTTGCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCggg                                                                                                             >  3:184263/1‑134 (MQ=255)
                tCACCGTAGGTCAGCCGCAGCGGTTCGGCGTCGCTGAACTTGATGCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAg                                                                                                         <  3:250577/135‑1 (MQ=255)
                     gTAGGTCAGCCGCAGCGGTTCGGCGTCGCTGAACTTGATGCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGGCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCt                                                                                                    <  8:200230/135‑1 (MQ=255)
                       aGGTCAGCCGCAGCGGTTCGGCGTCGCTGAACTTGATGCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTgc                                                                                                  >  4:1174/1‑135 (MQ=255)
                           cAGCCGCAGCGGTTCGGCGTCGCTGAACTTGATGCCGTCGAACGACCACGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATTGCGGGCGACCGGCCGGGTACGGGTAGGGGCTGCGCAg                                                                                              >  4:62251/1‑135 (MQ=255)
                                 cAGCGGTTCGGCGTCGCTGAACTTGATGCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCTATGTCACGCGATGGCGGGCGACTGTCCgg                                                                                                                   >  5:152147/1‑108 (MQ=255)
                                 cAGCGGTTCGGCGTCGCTGAACTTGATGCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACTGTCCgg                                                                                                                   <  6:156806/108‑1 (MQ=255)
                                 cAGCGGTTCGGCGTCGCTGAACTTGATGCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACTGTCCgg                                                                                                                   >  5:156806/1‑108 (MQ=255)
                                 cAGCGGTTCGGCGTCGCTGAACTTGATGCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACTGTCCgg                                                                                                                   <  6:152147/108‑1 (MQ=255)
                                 cAGCGGTTCGGCGTCGCTGAACTTGATGCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAg                                                                                                         >  4:170987/1‑118 (MQ=255)
                                 cAGCGGTTCGGCGTCGCTGAACTTGATGCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAg                                                                                                         <  3:170987/118‑1 (MQ=255)
                                 cAGCGGTTCGGCGTCGCTGAACTTGATGCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGc                                                                                        >  7:169666/1‑135 (MQ=255)
                                                       ttGATGCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCa                                                                  <  1:223426/135‑1 (MQ=255)
                                                       ttGATGCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGGCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGGACGCGGCGACCa                                                                  <  6:228017/135‑1 (MQ=255)
                                                            gCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTc                                                             <  8:169666/135‑1 (MQ=255)
                                                            gCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTc                                                             <  3:219719/135‑1 (MQ=255)
                                                            gCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGCAGGTCAGCACGCGGCGACCATTGTc                                                             >  5:155447/1‑135 (MQ=255)
                                                              cGTCGCACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCa                                                            <  5:235029/134‑1 (MQ=255)
                                                              cGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTc                                                             <  3:1174/133‑1 (MQ=255)
                                                              cGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGGCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTc                                                             <  2:100389/133‑1 (MQ=255)
                                                                 cGAACGACCAGGCGACGCGCTCCATATGGCCGGTCAGGGGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCa                                                        <  6:178239/135‑1 (MQ=255)
                                                                  gAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGGCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCAg                                                       <  5:286206/135‑1 (MQ=255)
                                                                   aaCGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCAg                                                       <  3:250799/134‑1 (MQ=255)
                                                                                   gcTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCAGGATCgg                                      >  8:219084/1‑135 (MQ=255)
                                                                                     tCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCAGGATCgg                                      >  2:276591/1‑133 (MQ=255)
                                                                                        atatGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCAGGATCGGCCAgg                                 >  6:134614/1‑135 (MQ=255)
                                                                                             gCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCAGGATCGGCCAGGTTggg                            <  1:276591/135‑1 (MQ=255)
                                                                                              ccGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGAGCGGCCGGGTCTGGGGAGGGGCTGCGCAGTTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCAGGATCGGACAGTTTggg                            <  2:257460/134‑1 (MQ=255)
                                                                                               cGGGCAGGTGCAGCTCGAGGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCAGGATCGGCCAGGTTGGGgc                          <  6:97320/135‑1 (MQ=255)
                                                                                                    aGGTGCAGCTCGATGTCACGCGATGGCGGGCGATCGTCCGGGTCGGGGTAGTGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCAGGATCGGCCAGGTTGGGGCGCGGt                     >  8:6333/1‑135 (MQ=255)
                                                                                                    aGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCAGGATCGGCCAGGTTggg                            >  3:1316/1‑128 (MQ=255)
                                                                                                    aGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCAGGATCGGCCAGGTTggg                            <  4:1316/128‑1 (MQ=255)
                                                                                                    aGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCAGGATCGGCCAGGTTGGGGCGCGGt                     >  2:10775/1‑135 (MQ=255)
                                                                                                    aGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCAGGATCGGCCAGGTTGGGGCGCGGt                     <  8:230700/135‑1 (MQ=255)
                                                                                                    aGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCAGGATCGGCCAGGTTGGGGCGCGGt                     >  7:69631/1‑135 (MQ=255)
                                                                                                    aGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCAGGATCGGCCAGGTTGGGGCGCGGt                     >  7:80737/1‑135 (MQ=255)
                                                                                                     ggTGCAGCTCGATGTCAGGCGAGGGCGGGCGACCGTCCGGGGCGGGGGAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCAGGATCGGCCAGGGTGGGGCGCGGTg                    <  5:178904/135‑1 (MQ=255)
                                                                                                     ggTGCAGCTCGATGTCACGCGATGGCGGGCGACCGGCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGGCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCAGGATCGGCCAGGGTGGGGCGCGGTg                    <  3:281246/135‑1 (MQ=255)
                                                                                                           gCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGGCGGGGTAGGGGCTGCGCAGGTCGGCGCAGGTCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCAGGATCGGCCAGGTTGGGGCGCGGTGCCATgg              <  6:155447/135‑1 (MQ=255)
                                                                                                             tCGATGTCACGCGATGGCGGGCGACCGTGCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCAGGATCGGCCAGGTTGGGGCGCGGTGCCATGGTc            <  6:81899/135‑1 (MQ=255)
                                                                                                                aTGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCAGGATCGGCCAGGTTGGGGCGCGGTGCCATGGTCTGc         <  5:134614/135‑1 (MQ=255)
                                                                                                                   tCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCAGGATCGGCCAGGTTGGGGCGCGGTGCCATGGTCTGCATg      >  1:246222/1‑135 (MQ=255)
                                                                                                                         gATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCAGGATCGGCCAGGTTGGGGCGCGGTGCCATGGTCTGCATGTcca  >  2:33727/1‑133 (MQ=255)
                                                                                                                         gATGGCGGGCGACCCTCCGGGTCGGGGTAGGGGCTGCCCAGGTCGCCGTAGGTCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCACGATCGGCCAGCTTGGGGCGCGGAGCCATGGTCTGCATGTcaa  >  7:175691/1‑131 (MQ=255)
                                                                                                                                      |                                                                                                                       
GGTGATGCGCACGCGCTCACCGTAGGTCAGCCGCAGCGGTTCGGCGTCGCTGAACTTGATGCCGTCGAACGACCAGGCGAAGCGCTCCATATGGCCGGTCAGGTGCAGCTCGATGTCACGCGATGGCGGGCGACCGTCCGGGTCGGGGTAGGGGCTGCGCAGGTCGGCGTAGGTCAGCACGCGGCGACCATTGTCACGCAGGCCTATGCCAGGATCGGCCAGGTTGGGGCGCGGTGCCATGGTCTGCATGTCCA  >  ACB122/2503294‑2503547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑