Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 2,517,936 C→T 8.5% P521L (CCG→CTG)  acsA_1 → Acetyl‑coenzyme A synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,517,9360CT 8.5% 131.5 / 3.4 49P521L (CCG→CTG) acsA_1Acetyl‑coenzyme A synthetase
Reads supporting (aligned to +/- strand):  ref base C (22/22);  new base T (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

GTTCGTGGTATTGGCCAGCGGCTACGCCGGCAGCGTCGAGCTGGAAGAAACCTTGCGCCAGCATGTGCGCCAGCGCCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTGAAAGGAAAGTACTCATGCACATAGCCAATAAACATTTCATCGTCAGCGG  >  ACB122/2517802‑2518069
                                                                                                                                      |                                                                                                                                     
gTTCGTGGTATTGGCCAGCGGCTACGCCGGCAGCGGCGAGCTGGAAGAAACCTTGCGCCAGCATGTGCGCCAGCGCCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGAcccc                                                                                                                                       <  5:110143/135‑1 (MQ=255)
     tGGTATTGGCCAGCGGCTACGCCGGCAGCGTCGAGCTGGAAGAAACCTTGCGCCAGCATGTGCGCCAGCGCCTCTACGCGCATGCCTACCCCAGGGAACTCGAATTCGTCAGCGCGCTGCCCAGGACCCCGAGCg                                                                                                                                  >  2:289903/1‑135 (MQ=255)
     tGGTATTGGCCAGCGGCTACGCCGGCAGCGTCGAGCTGGAAGAAACCTTGCGCCAGCATGTGCGCCAGCGCCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCg                                                                                                                                  >  7:289330/1‑135 (MQ=255)
     tGGTATTGGCCAGCGGCTACGCCGGCAGCGTCGAGCTGGAAGAAACCTTGCGCCAGCATGTGCGCCAGCGCCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCg                                                                                                                                  >  7:149217/1‑135 (MQ=255)
     tGGTATTGGCCAGCGGCTACGCCGGCAGCGTCGAGCTGGAAGAAACCTTGCGCCAGCATGTGCGCCAGCGCCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGAACCCGAGCg                                                                                                                                  >  2:39575/1‑135 (MQ=255)
      ggTATTGGCCAGCGGCTACGCCGGCAGCGTCGAGCTGGAAGAAACCTTGCGCCAGCATGTGCGCCAGCGCCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGCCCCCGAGCg                                                                                                                                  >  4:95747/1‑134 (MQ=255)
            ggCCAGCGGCTACGCCGGCAGCGTCGAGCTGGAAGAAACCTTGCGCCAGCATGTGCGCCAGCGCCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCt                                                                                                                           <  7:70946/135‑1 (MQ=255)
             gCCAGCGGCTACGCCGGCAGCGTCGAGCTGGAAGAAACCTTGCGCCAGCATGTGCGCCAGCGCCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCt                                                                                                                           <  3:6531/134‑1 (MQ=255)
                     cTACGCCGGCAGCGTCGAGCTGGAAGAAACCTTGCGCCAGCATGTGCGCCAGCGCCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGCGCGGCAAGCTGCAACGCt                                                                                                                   >  7:184571/1‑134 (MQ=255)
                     cTACGCCGGCAGCGTCGAGCTGGAAGAAACCTTGCGCCAGCATGTGCGCCAGCGCCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCt                                                                                                                   >  4:119943/1‑134 (MQ=255)
                     cTACGCCGGCAGCGTCGAGCTGGAAGAAACCTTGCGCCAGCATGTGCGCCAGCGCCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCt                                                                                                                   >  4:97993/1‑134 (MQ=255)
                         gCCGGCAGCGTCGAGCTGGAAGAAACCTTGCGCCAGCTTGTGCGCCAGCGCCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCATGACCCCGAGCGGCAAGCTGCAACGCTTCATc                                                                                                              <  6:29195/135‑1 (MQ=255)
                           cGGCAGCGTCGAGCTGGAAGAAACCTTGCGCCAGCATGTGCGCCAGCGCCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCt                                                                                                            <  4:200668/135‑1 (MQ=255)
                           cGGCAGCGTCGAGCTGGAAGAAACCTTGCGCCAGCATGTGCGCCAGCGCCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCt                                                                                                            <  5:171720/135‑1 (MQ=255)
                               aGCGTCGAGCTGGAAGAAACCTTGCGCCAGCATGTGCGCCAGCGCCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCg                                                                                                                                  >  6:152197/1‑109 (MQ=255)
                               aGCGTCGAGCTGGAAGAAACCTTGCGCCAGCATGTGCGCCAGCGCCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCg                                                                                                                                  <  5:152197/109‑1 (MQ=255)
                                    cGAGCTGGAAGAAACCTTGCGCCAGCATGTGCGCCAGCGCCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCa                                                                                                   <  3:97993/135‑1 (MQ=255)
                                        cTGGAAGAAACCTTGCGCCAGCATGTGCGCCAGCGCCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGaa                                                                                               >  8:181789/1‑135 (MQ=255)
                                                       agcCAGCATGTGCGCGAGCGCCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACGCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAaacaac                                                                                  <  8:72371/132‑1 (MQ=255)
                                                        gcCAGCATGTGCGCCAGCGCCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAg                                                                               >  5:211257/1‑135 (MQ=255)
                                                               tgtgCGCCAGCGCCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACa                                                                        >  1:79568/1‑135 (MQ=255)
                                                                         cgcCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCt                                                                                                                           <  2:252998/74‑1 (MQ=255)
                                                                         cgcCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCt                                                                                                                           >  1:252998/1‑74 (MQ=255)
                                                                           ccTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCACGCTGCAACGCTTCCTCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCACCAGGCCACCCCTGc                                                            >  2:79670/1‑135 (MQ=255)
                                                                                  gcgcCTGCCTACCCACGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTc                                                     <  8:208481/135‑1 (MQ=255)
                                                                                  gcgcATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTc                                                     <  8:212821/135‑1 (MQ=255)
                                                                                     cATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTga                                                  <  7:290020/135‑1 (MQ=255)
                                                                                       tGCCTACCCCACGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTgaaa                                                <  3:22026/135‑1 (MQ=255)
                                                                                          gtACACCAGGGAAATCGAATTCGTCAGCGAGCTGCCCACGACCCCGATCGGCAAGCTGCAACGCTTCATCCTGCGCAAACAGGAAGGCGCAAAAAAACAAGCTCAACAGGCCACCCCTGCCATCGTCTgaaatga                                             <  3:157034/134‑1 (MQ=255)
                                                                                          cTACCGCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCGGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAAAAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTgaaagga                                             <  1:57439/135‑1 (MQ=255)
                                                                                          cTACCCCAGGGAATTCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTgaaagga                                             <  5:277419/135‑1 (MQ=255)
                                                                                          cTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTgaaagga                                             <  4:111967/135‑1 (MQ=255)
                                                                                          cTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTgaaagga                                             <  3:119943/135‑1 (MQ=255)
                                                                                            aCCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTgaaaggaaa                                           >  3:50508/1‑135 (MQ=255)
                                                                                              cgaaGGGAATTCGAATTCGTCAGGGAGCGGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTGAAAGGAAAGt                                         <  1:39575/132‑1 (MQ=255)
                                                                                                cAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTGAAAGGAAAGTAc                                       <  6:211257/135‑1 (MQ=255)
                                                                                                     aaatcgaaatcgTCAGCGAGCTGCACAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTGAAAGGAAAGTACTCATg                                  >  4:108588/1‑135 (MQ=255)
                                                                                                     aaaTCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTGAAAGGAAAGTACTCAt                                   >  7:43548/1‑134 (MQ=255)
                                                                                                     aaaTCGAATTCGTAAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGAAACCAGGAAGTCGCAAAACAACAAGCGCAACAGGCCACCCCTGCCATCGTCTGAAAGGAAAGTACTCAt                                   >  4:157034/1‑134 (MQ=255)
                                                                                                      aaTCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTgaaagga                                             >  8:279563/1‑123 (MQ=255)
                                                                                                      aaTCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTgaaagga                                             <  7:279563/123‑1 (MQ=255)
                                                                                                       ttggAATTCGTCAGCGAGGTGGCCAAGACCCCGAGGGGCAAGCTGCATCGCTTCATGCTGCGCTACCAGGAAGTCGCCAATCAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTGAAAGGAAAGTACTCATGca                                <  1:301434/132‑1 (MQ=255)
                                                                                                          gAATTCGTCAGCGAGCTGCCCAAGACCCTGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTGAAAGGAAAGTACTCATGcac                               >  4:200148/1‑133 (MQ=255)
                                                                                                          gAATTCGTCAGCGAGCTGCCCAAGACCCTGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTGAAAGGAAAGTACTCATGcac                               >  4:203916/1‑133 (MQ=255)
                                                                                                           aaTTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTc                                                     >  2:216059/1‑110 (MQ=255)
                                                                                                           aaTTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTc                                                     <  1:216059/110‑1 (MQ=255)
                                                                                                           aaTTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTGAAAGGAAAGTACTCATGCACATa                            >  7:302453/1‑135 (MQ=255)
                                                                                                           aaTTCGTCAGCGAGCTGCCCAAGACCCCGAGCGCCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTGAAAGGAAAGTACTCATGCACATa                            >  1:189375/1‑135 (MQ=255)
                                                                                                              tCGTCAGCGAGCTGCCCAAGACCCTGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTGAAAGGAAAGTACTCATGCACATAGcc                         <  3:203916/135‑1 (MQ=255)
                                                                                                              tCGTCAGCGAGCTGCCCAAGACCCTGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTGAAAGGAAAGTACTCATGCACATAGcc                         <  3:200148/135‑1 (MQ=255)
                                                                                                                                     ccGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTGAAAGGAAAGTACTCATGCACATAGCCAATAAACATTTCATCGTCAgcgg  <  1:31720/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                     
GTTCGTGGTATTGGCCAGCGGCTACGCCGGCAGCGTCGAGCTGGAAGAAACCTTGCGCCAGCATGTGCGCCAGCGCCTCTACGCGCATGCCTACCCCAGGGAAATCGAATTCGTCAGCGAGCTGCCCAAGACCCCGAGCGGCAAGCTGCAACGCTTCATCCTGCGCAACCAGGAAGTCGCCAAACAACAAGCGCAACAGGCCACCCCTGCCAGCGTCTGAAAGGAAAGTACTCATGCACATAGCCAATAAACATTTCATCGTCAGCGG  >  ACB122/2517802‑2518069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑