Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 2,958,255 G→T 7.3% Q477K (CAG→AAG)  irtB ← Iron import ATP‑binding/permease protein IrtB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,958,2550GT 7.3% 173.8 / 3.3 55Q477K (CAG→AAG) irtBIron import ATP‑binding/permease protein IrtB
Reads supporting (aligned to +/- strand):  ref base G (25/26);  new base T (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

GGTGCAACGCCCATGCAGGCTGTGCAGCGTCTGGGCAATCACGGCCTGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGG  >  ACB122/2958121‑2958386
                                                                                                                                      |                                                                                                                                   
ggTGCAACGCCCATGCAGGCTGTGCAGCGTCTGGGCAATCACGGCCTGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTg                                                                                                                                     >  2:205170/1‑135 (MQ=255)
     aaCGCCCATGCAGGCTGTGCAGCGTCTGGGCAATCACGGCCTGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGtt                                                                                                                                <  1:32119/135‑1 (MQ=255)
     aaCGCCCATGCAGGCTGTGCAGCGTCTGGGCAATCACGGCCTGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGtt                                                                                                                                <  1:93221/135‑1 (MQ=255)
       cGCCCATGCAGGCTGTGCAGCGTCTGGGCAATCACGGCCAGGTTCTCGGCAGCAAGGGCGGCGGTGGCTTCGTCCCCCAGCCGTACTGGTGCTTGCCTGATCAGCGCCCGGGCAATGGTCACCCGCTGGCGTTCg                                                                                                                              >  3:262819/1‑135 (MQ=255)
          ccATGCAGGCTGTGCAGCGTCTGGGCAATCACGGCCTGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAAGGGTCACACGCTGGCGTTCGCCa                                                                                                                           >  2:172197/1‑135 (MQ=255)
               cAGGCTGTGCAGCGTCTGGGCAATCACGGCCTGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCCGCAGTACTGGTGCTTGCCGGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCgg                                                                                                                       >  1:119364/1‑134 (MQ=255)
                aGGCTGTGCAGCGTCTGGGCAATCACGGCCTGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCTCGGGCAATGGTCACACGCTGGCGTTCGCCACCGGa                                                                                                                      <  3:118528/134‑1 (MQ=255)
                  gCTGTGCAGCGTCTGGGCAATCACGGCCTGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAg                                                                                                                   <  7:95910/135‑1 (MQ=255)
                      tgCAGCGTCTGGGCAATCACGGCCTGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTgc                                                                                                               <  3:284204/135‑1 (MQ=255)
                      tgCAGCGTCTGGGCAATCACGGCCTGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTgc                                                                                                               <  6:165470/135‑1 (MQ=255)
                       gCAGCGTCTGGGCAATCACGGCCTGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTgc                                                                                                               <  7:194905/134‑1 (MQ=255)
                       gCAGCGTCTGGGCAATCACGGCCTGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTgc                                                                                                               <  4:57639/134‑1 (MQ=255)
                         aGCGTCTGGGCAATCACGGCCTGGTTCTCGTCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGAACGGGCAATGGTCACACGCTTGCGTTCGCCACCGGAAAGCTGCGca                                                                                                            >  4:189729/1‑135 (MQ=255)
                         aGCGTCTGGGCAATCACGGCCTGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTTGCGTTCGCCACCGGAAAGCTGCGca                                                                                                            >  4:189726/1‑135 (MQ=255)
                         aGCGTCTGGGCAATCACGGCCTGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGca                                                                                                            >  8:282170/1‑135 (MQ=255)
                             tCTGGGCAATCACGGCCTGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGcaccac                                                                                                        <  6:89190/135‑1 (MQ=255)
                                 ggCAATCACGGCCTGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTTGCGTTCGCCACCGGAAAGCTGCGCACCACCTTc                                                                                                    <  3:189726/135‑1 (MQ=255)
                                  gCAATCACGGCCTGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTTGCGTTCGCCACCGGAAAGCTGCGCACCACCTTc                                                                                                    <  3:189729/134‑1 (MQ=255)
                                   cAATCACGGCCTGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCCCCTTCGc                                                                                                  >  5:139363/1‑135 (MQ=255)
                                   cAATCACGGCCTGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGc                                                                                                  >  1:96573/1‑135 (MQ=255)
                                              tGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCCCACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCCCGGACGTa                                                                                       >  5:170300/1‑135 (MQ=255)
                                              tGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTa                                                                                       >  2:6449/1‑135 (MQ=255)
                                              tGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTa                                                                                       >  1:220099/1‑135 (MQ=255)
                                                       gCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGGCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAgg                                                                                 <  7:209162/132‑1 (MQ=255)
                                                             aGGGTGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCcgcg                                                                         <  1:162598/134‑1 (MQ=255)
                                                             aGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCcgcg                                                                         <  7:117829/134‑1 (MQ=255)
                                                             aGGGCGGCGGTGGCGTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCcgcg                                                                         <  1:83614/134‑1 (MQ=255)
                                                              gggCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCa                                                                       >  3:247226/1‑135 (MQ=255)
                                                              gcgcggcgGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCa                                                                       <  6:65414/133‑1 (MQ=255)
                                                               ggcggcGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCgcgc                                                                        <  8:183105/133‑1 (MQ=255)
                                                                        ggCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAg                                                             <  3:214043/135‑1 (MQ=255)
                                                                        ggCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAg                                                             <  5:155109/135‑1 (MQ=255)
                                                                                 caccaGCAGTACTGGTGCTTGCCTGATCAGCGCCCGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTc                                                     >  4:221688/1‑134 (MQ=255)
                                                                                  cacagcagTACTGGGGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTccc                                                   <  6:240251/133‑1 (MQ=255)
                                                                                               gTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCAtgt                                      >  7:303118/1‑135 (MQ=255)
                                                                                               gTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCAtgt                                      <  2:220099/135‑1 (MQ=255)
                                                                                                tGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGGTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTCCCAAGCCAGCCAtgt                                      <  3:9015/134‑1 (MQ=255)
                                                                                                 gCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTgc                                    <  5:263786/135‑1 (MQ=255)
                                                                                                 gCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTgc                                    >  8:112555/1‑135 (MQ=255)
                                                                                                 gCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTgc                                    >  7:77804/1‑135 (MQ=255)
                                                                                                 gCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTgc                                    <  2:78055/135‑1 (MQ=255)
                                                                                                       cTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGc                                                                                                  >  7:232424/1‑67 (MQ=255)
                                                                                                       cTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGc                                                                                                  <  8:232424/67‑1 (MQ=255)
                                                                                                       cTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCt                              >  3:270712/1‑135 (MQ=255)
                                                                                                       cTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCt                              >  8:33822/1‑135 (MQ=255)
                                                                                                        tGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCtt                             <  4:146138/135‑1 (MQ=255)
                                                                                                         gATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCtt                             >  7:110074/1‑134 (MQ=255)
                                                                                                             aGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGt                        >  1:26720/1‑135 (MQ=255)
                                                                                                             aGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTCCCAAGACGGCCATGTGCGCCGCTTGCAGt                        >  3:33289/1‑135 (MQ=255)
                                                                                                                      gCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCg               <  4:263170/135‑1 (MQ=255)
                                                                                                                      gCAATGGGCACACGCTGGCGTTCGCCAGCGGAAAGCTGCGCACCACCTTCGCCGACGGACGTATCGAGGCCCCCGCGCAGGCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGATTGCAGTACCTCGGCg               <  4:267542/135‑1 (MQ=255)
                                                                                                                            gTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTg         >  2:41456/1‑135 (MQ=255)
                                                                                                                                   tcaggCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCAGGGACGTATCGCTGCACCCGCGCAGCCGATCTAGGGTGTGTCCCAAGCCGGCCATGAGCGGCGCTTGCCGTCCCTCGGCGTCGCTGGCATCgg  >  8:84290/4‑135 (MQ=255)
                                                                                                                                   gCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGTCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCgg  >  2:30908/1‑135 (MQ=255)
                                                                                                                                   gCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCgg  >  4:55579/1‑135 (MQ=255)
                                                                                                                                   gCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCgg  >  3:231276/1‑135 (MQ=255)
                                                                                                                                   gCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCGTGCCGTACCTCGGCGTCGCGGGCCTCgg  >  2:141687/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                   
GGTGCAACGCCCATGCAGGCTGTGCAGCGTCTGGGCAATCACGGCCTGGTTCTCGGCATCAAGGGCGGCGGTGGCTTCGTCCACCAGCAGTACTGGTGCTTGCCTGATCAGCGCACGGGCAATGGTCACACGCTGGCGTTCGCCACCGGAAAGCTGCGCACCACCTTCGCCCACGGACGTATCGAGGCCCCCGCGCAGCCGTTCGAGGGTGTGTCCCAAGCCGGCCATGTGCGCCGCTTGCAGTACCTCGGCGTCGCTGGCATCGG  >  ACB122/2958121‑2958386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑