Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,065,508 G→A 100% V53M (GTG→ATG)  rcsC_6 → Sensor histidine kinase RcsC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,065,5080GA100.0% 126.8 / NA 37V53M (GTG→ATG) rcsC_6Sensor histidine kinase RcsC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/21);  total (16/21)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGGGCGTTGGCCGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCGTGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTT  >  ACB122/3065379‑3065638
                                                                                                                                 |                                                                                                                                  
cGGGCGTTGGCCGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGCGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCAc                                                                                                                               >  1:293991/1‑135 (MQ=255)
  ggCGTTGGCCGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCGGGACGAGCTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTAGAAAACGCCATGCACgg                                                                                                                             >  5:289540/1‑135 (MQ=255)
      ttGGCGGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTAtt                                                                                                                         <  5:176497/135‑1 (MQ=255)
           cGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCa                                                                                                                    <  7:131396/135‑1 (MQ=255)
             ggggggtgggggtgggTGACCACTCGGAGCGTGAAAGGCAATAACCGGCTCTGTCCGGCCGCCTGGTCGTACTGGAAGCCGAACGGCACCGGTACAAATGGCTGTTCGTAAACGGCATGCACGGTATTTTCCAgg                                                                                                                  <  2:287488/129‑1 (MQ=255)
                tgctgGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAACACGCCATGCACGGTATTTTCCAGGCCa                                                                                                               >  7:189788/1‑135 (MQ=255)
                tgctgGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCa                                                                                                               >  7:185139/1‑135 (MQ=255)
                             tGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACgg                                                                                                  >  1:81701/1‑135 (MQ=255)
                               aCCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCa                                                                                                                    <  4:295134/115‑1 (MQ=255)
                               aCCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCa                                                                                                                    >  3:295134/1‑115 (MQ=255)
                               aCCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCAGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCa                                                                                                >  3:4748/1‑135 (MQ=255)
                                ccACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCAt                                                                                               <  2:81701/135‑1 (MQ=255)
                                ccACTCGGCGCGTAAAAGCCAGTCCCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCAt                                                                                               <  5:31084/135‑1 (MQ=255)
                                        cgcgTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGCCGAACTGGAAGCCGCACGCAACAGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTACAGGCCAGCCTTCAGGACGGCATGCGTgccg                                                                                       >  2:59406/1‑135 (MQ=255)
                                           gTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCa                                                                                    <  7:178001/135‑1 (MQ=255)
                                           gTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCa                                                                                    <  8:49304/135‑1 (MQ=255)
                                           gTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGAACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCa                                                                                    <  7:106341/135‑1 (MQ=255)
                                                          cGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGgcgc                                                                     >  7:293106/1‑135 (MQ=255)
                                                            gAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGgcgcgc                                                                   <  6:110512/135‑1 (MQ=255)
                                                              gCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTAGAAATGGCTGATCGATAACGCCATGCAGGGTATTTTCCAGGCCAGCCTTCAGGACGGCAGGCGTGCCGCCAACCCGGCGCTGGCGCGCAt                                                                 <  6:268389/135‑1 (MQ=255)
                                                              gCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCAt                                                                 <  1:287773/135‑1 (MQ=255)
                                                              gCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCAt                                                                 <  1:99070/135‑1 (MQ=255)
                                                              gCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCAt                                                                 <  1:284138/135‑1 (MQ=255)
                                                                     gcccgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCgg                                                           >  4:186720/1‑134 (MQ=255)
                                                                     gcccgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCgg                                                           >  3:253039/1‑134 (MQ=255)
                                                                     gcccgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGc                                                          >  2:205056/1‑135 (MQ=255)
                                                                     gcccgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCAAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCgg                                                           >  5:130032/1‑134 (MQ=255)
                                                                       ccgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTa                                                        <  6:130032/135‑1 (MQ=255)
                                                                          ccTGGAGGAACTGTAAGCCGAACGCAACCGTTACAAATGGATGTTCGAAAAGGCCATGCAGGGTATTTTCCTGGCCAGCGTTGAGGACGGAATGCGTGCGGCAAACCCGGCGCTGGCGCGCATGCTCGGCTATga                                                     <  6:259216/135‑1 (MQ=255)
                                                                                      ggAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGc                                         >  3:194152/1‑135 (MQ=255)
                                                                                        aaGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGAACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGc                                         <  3:288157/133‑1 (MQ=255)
                                                                                               aCGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGTACGGCATGCGTGCCGCCACCCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGtt                                <  4:232827/135‑1 (MQ=255)
                                                                                                 gCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGtt                                <  4:140326/133‑1 (MQ=255)
                                                                                                 gCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGtt                                >  3:140326/1‑133 (MQ=255)
                                                                                                            aaaTGGCTGTTCGAAAAGGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCctgactga                    <  4:4748/134‑1 (MQ=255)
                                                                                                                        gAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAAc       >  3:280746/1‑135 (MQ=255)
                                                                                                                             cGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGtt  <  7:19922/135‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                  
CGGGCGTTGGCCGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCGTGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTT  >  ACB122/3065379‑3065638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑