Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	3,117,533	A→T	6.1%	G259G (GGT→GGA)	CJ019_02936 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	3,117,533	0	A	T	6.1%	207.4 / 3.7	66	G259G (GGT→GGA)	CJ019_02936	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (20/42);  new base T (2/2);  total (22/44)
Fisher's exact test for biased strand distribution p-value = 5.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

TTTCAGCGGTCAGCCATTTCAACGTGCCCGGGCGTGCCATGCGCCTTAGCACACGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAGACCTTGTGTAGGGGAACTGAGACAGCACGAAGCCCAGCCACTGGTAGCAGAACAACAACACGACCAACCAG  >  ACB122/3117400‑3117662                                                                                                                                      |                                                                                                                                  tttCAGCGGTCAGCCATTTCAACGTGCCCGGGCGTGCCATGCGCCTTAGCACACGCTTGGAGACGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCAGGCATGGCGCGCAAAATAc                                                                                                                                  <  4:140572/135‑1 (MQ=255) tttCAGCGGTCAGCCATTTCAACGTGCCCGGGCGGGCCATGCGCCTTAGCACACGCTTGGAGACGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATAc                                                                                                                                  <  5:168770/135‑1 (MQ=255)      gCGGTCAGCCATTTCAACGTGCCCGGGCGTGCCATGCGCCTTAGCACACGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATTCCGCTg                                                                                                                             >  7:160729/1‑135 (MQ=255)      gCGGTCAGCCATTTCAACGTGCCCGGGCGTGCCATGCGCCTTAGCACACGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTg                                                                                                                             >  1:180229/1‑135 (MQ=255)         ggcAGCCATTTCAACGTGCCCGGGCGTGCCATGCGCCTTAGCACACGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATc                                                                                                                          <  3:37449/133‑1 (MQ=255)            aGCCATTTCAACGTGCCCGGGCGTGCCATGCGCCTTAGCACACGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAgg                                                                                                                       <  1:175540/135‑1 (MQ=255)            aGCCATTTCAACGTGCCCGGGCGTGCCATGCGCCTTAGCACACGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAgg                                                                                                                       <  4:248743/135‑1 (MQ=255)              ccATTTCAACGTGCCCGGGCGTGCCATGCGCCTTAGCACACGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTa                                                                                                                     >  5:143752/1‑135 (MQ=255)              ccATTTCAACGTGCCCGGGCGTGCCATGCGCCTTAGCACACGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTa                                                                                                                     >  3:26836/1‑135 (MQ=255)                     aaCGTGCCCGGGCGTGCCATGCGCCTTAGCACACGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATTCCGCTGATCAGGTAGTTGag                                                                                                               <  8:160729/134‑1 (MQ=255)                      aCGTGCCCGGGCGTGCCATGCGCCTTAGCACACGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGagca                                                                                                             >  8:211535/1‑135 (MQ=255)                              gggCGTGCCATGCGCCTTAGCACACGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCa                                                                                                       >  5:248025/1‑133 (MQ=255)                               ggCGTGCCATGCGCCTTAGCACACGCTTGGATAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGACAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCa                                                                                                    <  1:40968/135‑1 (MQ=255)                               ggCGTGCCATGCGCCTTAGCACACGCTTGGAGAAGGCGCTGATGCCACGGGCGTTGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCa                                                                                                    <  7:216502/135‑1 (MQ=255)                               ggCGTGCCATGCGCCTTAGCACACGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCa                                                                                                    <  4:70749/135‑1 (MQ=255)                               ggCGTGCCATGCGCCTTAGCACACGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCa                                                                                                    <  7:91359/135‑1 (MQ=255)                                gCGTGCCTTGCGCCTTAGCAAAGGTTGGGAGAAGGCGGGTATGCCACGGGTGATTAAGAAGATGGACAGTGCTACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAg                                                                                                   <  5:17084/135‑1 (MQ=255)                                gCGTGCCATGCGCCTTAGCACACGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAg                                                                                                   <  2:239012/135‑1 (MQ=255)                                        agagcCTTAGCACACGCTTGGAGAAGGCGCAGATGCCACGGGCGATGAAGAAGATCGAAACTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACAGCTGATCAGCTAGTTGAGCAGCGCCATCAGGAGGctaa                                                                                           >  2:128851/4‑134 (MQ=255)                                            ccTTAGCACACGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAg                                                                                       <  2:247514/135‑1 (MQ=255)                                              ttAGCACACGCTTGGCTAAGGCGCTGATGCCACGGGCGTTGATTAAGATCGACAGTGCTACGGCCAGGCCGGGCATGGCGCGCTAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGc                                                                                     <  5:234074/135‑1 (MQ=255)                                              ttAGCACACGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGc                                                                                     <  8:1820/135‑1 (MQ=255)                                              ttAGCACACGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGc                                                                                     <  3:219991/135‑1 (MQ=255)                                              ttAGCACACGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGc                                                                                     <  7:146377/135‑1 (MQ=255)                                                  cacaCGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTc                                                                                 >  4:158109/1‑135 (MQ=255)                                                   acacGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTcc                                                                                >  3:110048/1‑135 (MQ=255)                                                     acGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTcccc                                                                              <  2:180229/135‑1 (MQ=255)                                                     acGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTcccc                                                                              <  5:141956/135‑1 (MQ=255)                                                          tGGTGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAg                                                                         <  7:112398/135‑1 (MQ=255)                                                          tGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAg                                                                         >  8:139852/1‑135 (MQ=255)                                                          tGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGACAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAg                                                                         >  6:197456/1‑135 (MQ=255)                                                            gagaAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAg                                                                         <  8:93714/133‑1 (MQ=255)                                                               aaGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGTCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGCTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAGACCtt                                                                    >  7:13202/1‑135 (MQ=255)                                                               aaGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAGACCtt                                                                    <  3:158109/135‑1 (MQ=255)                                                                 ggCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAGACCTtgt                                                                  <  5:71340/135‑1 (MQ=255)                                                                   cgcTGATGCCCCGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAGACCTTgtgt                                                                <  6:203911/135‑1 (MQ=255)                                                                   cgcTGATGCCACGGGCTATGAATAAGATCGTCAGTGCAACGGCCTGGCCGGGCATGGCGCGCAATATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCGAAGACCTTgtgt                                                                <  2:280111/135‑1 (MQ=255)                                                                   cgcTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAGACCTTgtgt                                                                <  8:15907/135‑1 (MQ=255)                                                                    gcTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATTCCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAg                                                                                       >  4:192475/1‑111 (MQ=255)                                                                    gcTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATTCCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAg                                                                                       <  3:192475/111‑1 (MQ=255)                                                                    gcTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAATACCTTgtgt                                                                <  3:228787/134‑1 (MQ=255)                                                                    gcTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAGACCTTgtgt                                                                >  4:228787/1‑134 (MQ=255)                                                                         tGCCACGGGGGATGAAGAAGAGCGACAGTGCAACGGCCAGGCCGGTCATGGCGCGCAAAATAGCGCTGATCAGGTAGTTGAGCAGCGCGATCAGGTGGATATTGAGGCTCTCCCCCCAAGACCTTGTGTAggg                                                            <  4:34039/133‑1 (MQ=255)                                                                            gaCGGGCGATGAAGAAGATCGACAGTGCAACGGCGAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTTAGGCTTTACCCCCAAGACCTTGTGTAGGGTAAc                                                        <  6:82998/133‑1 (MQ=255)                                                                             aCGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAGACCTTGTGTAGGGGAACTg                                                      <  5:111271/135‑1 (MQ=255)                                                                                   gATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTT‑CCCCCAAGACCTTGTGTAGGGGAACTGAGACAGc                                               <  2:79439/135‑1 (MQ=255)                                                                                       aagaagATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAGACCTTGTGTAGGGGAACTGAGACAGCAc                                             >  8:201458/1‑134 (MQ=255)                                                                                        agaagaTCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAGACCTTgtgt                                                                <  7:178429/114‑1 (MQ=255)                                                                                        agaagaTCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAGACCTTgtgt                                                                >  8:178429/1‑114 (MQ=255)                                                                                         gaagaTGGTCAGTGCAAGGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATTTTGAGGCTTTGCCCCCAAGACCTTGTGTAGGGGAACTGAGACAGCACGaa                                          <  8:13202/135‑1 (MQ=255)                                                                                         gaagaTCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCTGGTGGATATTGAGGCTTTCCCCCCAAGACCTTGTGTAGGGGAACTGAGACAGCACGaa                                          <  5:197646/135‑1 (MQ=255)                                                                                               cGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAGACCTTGTGTAGGGGCACTGAGACAGCACGAAGCCCAg                                    >  7:44868/1‑135 (MQ=255)                                                                                                gACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCaa                                                                          >  2:280873/1‑96 (MQ=255)                                                                                                gACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCaa                                                                          <  1:280873/96‑1 (MQ=255)                                                                                                  cAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGc                                                                                                                               >  1:179821/1‑41 (MQ=255)                                                                                                  cAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGc                                                                                                                               <  2:179821/41‑1 (MQ=255)                                                                                                    gggCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCGGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAGACCTTGTGTAGGGGAACTGAGACAGCACGAAGCCCAGCCACt                               <  5:242281/133‑1 (MQ=255)                                                                                                            gCCAGGCCGGGCATGGCGCGAAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAGACCTTGTGTAGGGGAACTGAGACAGCACGAAGCCCAGCCACTGGTAGCAg                       <  5:294550/135‑1 (MQ=255)                                                                                                                 gCCGGGCATGGCGCGCAAAATACGGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAGACCTTGTGTAGGGGAACTGAGACAGCACGAAGCCCAGCCACTGGTAGCAGaacaa                  <  8:210552/135‑1 (MQ=255)                                                                                                                  ccGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAGACCTTGTGTAGGGGAACTGAGACAGCACGAAGCCCAGCCACTGGTAGCAGaacaa                  >  1:79849/1‑134 (MQ=255)                                                                                                                  ccGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAGACCTTGTGTAGGGGAACTGAGACAGCACGAAGCCCAGCCACTGGTAGCAGaacaa                  >  3:179907/1‑134 (MQ=255)                                                                                                                    gggCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGCGCAGCGCCATCAGGTGGAGATTGAGGCTTTCCCCCCAAGACCTTGTGTAGGGGACCTGAGACAGCACGAAGCCCAGCCACTGGTAGCAGaacaacaa               >  1:110122/1‑135 (MQ=255)                                                                                                                        aTGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCAATCAGGTGGATATTGAGGCTTTCCCCCCAAGACCTTGTGTAGGGGAACTGAGACAGCACGAAGCCCAGCCACTGGTAGCAGAACAACAACACg           <  1:142462/135‑1 (MQ=255)                                                                                                                            cgcgcAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAGACCTTGTGTAGGGGAACTGAGACAGCACGAAGCCCAGCCACTGGTAGCAGAACAACAACACGacc        <  7:201458/134‑1 (MQ=255)                                                                                                                                 aaaaTACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAGACCTTGTGTAGGGGAACTGAGACAGCACGAAGCCCAGCCACTGGTAGCAGAACAACAACACGACCAACCAg  <  4:110048/135‑1 (MQ=255)                                                                                                                                 aaaaTACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAGACCTTGTGTAGGGGAACTGAGACAGCACGAAGCCCAGCCACTGGTAGCAGAACAACAACACGACCAACCAg  <  7:139852/135‑1 (MQ=255)                                                                                                                                      |                                                                                                                                  TTTCAGCGGTCAGCCATTTCAACGTGCCCGGGCGTGCCATGCGCCTTAGCACACGCTTGGAGAAGGCGCTGATGCCACGGGCGATGAAGAAGATCGACAGTGCAACGGCCAGGCCGGGCATGGCGCGCAAAATACCGCTGATCAGGTAGTTGAGCAGCGCCATCAGGTGGATATTGAGGCTTTCCCCCCAAGACCTTGTGTAGGGGAACTGAGACAGCACGAAGCCCAGCCACTGGTAGCAGAACAACAACACGACCAACCAG  >  ACB122/3117400‑3117662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑