Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,146,945 T→A 7.8% F31Y (TTC→TAC)  CJ019_02964 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,146,9450TA 7.8% 157.8 / 4.2 51F31Y (TTC→TAC) CJ019_02964hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (27/20);  new base A (2/2);  total (29/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

CTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTACCTCAAGTACGTCAACGCCCAGGGCGGCGTGAATGGCGTCAAGCTGACCTGGAGCGAGT  >  ACB122/3146815‑3147076
                                                                                                                                  |                                                                                                                                   
cTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCTACGAACAGTATTTcccc                                                                                                                                 <  4:210672/135‑1 (MQ=255)
      ccACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGCACAGTATTTCCCCCTGCag                                                                                                                           >  8:56070/1‑135 (MQ=255)
       cACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCag                                                                                                                           <  5:122650/134‑1 (MQ=255)
            cccACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTAc                                                                                                                     >  1:221713/1‑135 (MQ=255)
            cccACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTAc                                                                                                                     >  5:248874/1‑135 (MQ=255)
            cccACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTAc                                                                                                                     >  2:183036/1‑135 (MQ=255)
             ccACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCCACGAACAGTATTTCCCCCTGCAGAGCTAcc                                                                                                                    >  8:89827/1‑135 (MQ=255)
             ccACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTAcc                                                                                                                    >  1:121013/1‑135 (MQ=255)
             ccACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTAcc                                                                                                                    >  1:108998/1‑135 (MQ=255)
             ccACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGAATTTCCCCCTGCAGAGCTAcc                                                                                                                    >  6:87219/1‑135 (MQ=255)
             ccACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCAAGCGGCCAACGAAAAGTATTTACCCCTGCAGAGCTAcc                                                                                                                    >  2:223565/1‑135 (MQ=255)
             ccACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCCGCCGCCTGGCGCTGGCCGTAAGCATAGCAGCCGGCCCAAGCAACGTGCACGCCGCCCACCCACAATAAATACCCCTGCAGAGCGAcc                                                                                                                    >  5:198776/1‑135 (MQ=255)
               aCTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACcgc                                                                                                                  <  1:96360/135‑1 (MQ=255)
                         aaaGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCt                                                                                                        >  6:292207/1‑135 (MQ=255)
                           aggaataCCACGATGTCGACCCGATCCGTCCTCGCCCGCCTGGCGCTGGACGTAAGCGTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTAt                                                                                                      <  5:70208/129‑1 (MQ=255)
                           aGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTAt                                                                                                      <  4:21612/135‑1 (MQ=255)
                                aaCCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTTCACGCGGCCAACGAACAGTATTACCCCCTGCAGAGCTACCGCGTTGGCCCCTATGcc                                                                                                   >  3:13833/1‑133 (MQ=255)
                                aaCCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTACCCCCTGCAGAGCTACCGCGGTGGCCCCTATGcc                                                                                                   >  3:10053/1‑133 (MQ=255)
                                                cGATCCGTCCTCGGCAGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCCACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCGATGCCGCAGGTGGTACAGGTttc                                                                                 >  7:300128/1‑135 (MQ=255)
                                                  aTCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTttctt                                                                               <  2:108998/135‑1 (MQ=255)
                                                  aTCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTttctt                                                                               <  3:216421/135‑1 (MQ=255)
                                                  aTCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTttctt                                                                               <  2:252219/135‑1 (MQ=255)
                                                          tCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACCTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGCGCTACCGCGTTGGCCCCGATGCCGCAGGTGGTACCGGTTTCTTCggtcggt                                                                       >  5:97496/1‑131 (MQ=255)
                                                               cgccTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTAt                                                                   >  5:247469/1‑134 (MQ=255)
                                                                   tGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGAtt                                                              <  4:227115/135‑1 (MQ=255)
                                                                    ggCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTa                                                             >  3:134403/1‑135 (MQ=255)
                                                                    ggCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTAAGCCGCAGGTGGTACCGGTTTCTTCGGGGGTTTTATCGATTa                                                             >  6:280403/1‑135 (MQ=255)
                                                                      cgcTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTAc                                                            <  5:279267/134‑1 (MQ=255)
                                                                        cTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCATGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTACCTc                                                         <  3:193664/135‑1 (MQ=255)
                                                                        cTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTACCTc                                                         <  2:8426/135‑1 (MQ=255)
                                                                              gTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTACCTCAAGTAc                                                   >  3:89368/1‑135 (MQ=255)
                                                                              gTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTGGTATCGAGTACCTCAAGtcc                                                   >  1:36604/1‑133 (MQ=255)
                                                                                   cTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTACCTCAAGTACGTCaa                                              >  2:36391/1‑135 (MQ=255)
                                                                                         tGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTACCTCAAGTACGTCAACGCCCa                                        >  1:303202/1‑135 (MQ=255)
                                                                                         tGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTACCTCAAGTACGTCAACGCCCa                                        >  5:76/1‑135 (MQ=255)
                                                                                                gcACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCagag                                                                                                                         <  5:184378/47‑1 (MQ=255)
                                                                                                gcACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCagag                                                                                                                         >  6:184378/1‑47 (MQ=255)
                                                                                                  aCTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTACCTCAAGTACGTCAACGCCCAGGGCGGCGt                               <  5:292207/135‑1 (MQ=255)
                                                                                                     cAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTACCTCAAGTACGTCAACGCCCAGGGCGGCGTGaa                            >  3:19057/1‑135 (MQ=255)
                                                                                                       aCGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTACCTCAAGTACGTCAACGCCCAGGGCGGCGTGAATg                          <  4:22101/135‑1 (MQ=255)
                                                                                                           gCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTACCTCAAGTACGTCAACGCCCAGGGCGGCGTGACTGGCGt                      >  6:165944/1‑135 (MQ=255)
                                                                                                             aCGCGGCCAACGAACAGTATTACCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTACCTCAAGTACGTCAACGCCCAGGGCGGCGTGAATGGCGTc                     <  4:13833/134‑1 (MQ=255)
                                                                                                             aCGCGGCCAACGAACAGTATTACCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTACCTCAAGTACGTCAACGCCCAGGGCGGCGTGAATGGCGTc                     <  4:10053/134‑1 (MQ=255)
                                                                                                              cgcgGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTACCTCAAGTACGTCAACGCCCAGGGCGGCGTGAATGGCGTCaa                   >  1:34699/1‑135 (MQ=255)
                                                                                                              cgcgGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGGTTCTTCGGTGGTTTTATCGATTACCTCAAGTACGTCAACGCCCAGGGCGGCGTGAATGGCGTCaa                   <  8:48641/135‑1 (MQ=255)
                                                                                                                 ggCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTACCTCAAGTACGTCAACGCCCAGGGCGGCGTGAATGGCGTCAAGc                 >  6:249942/1‑134 (MQ=255)
                                                                                                                   ccAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTACCTCAAGTACGTCAACGCCCAGGGCGGCGTGAATGGCGTCAAGCTGa              >  3:267326/1‑135 (MQ=255)
                                                                                                                             gTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTACCTCAAGTACGTCAACGCCCAGGGCGGCGTGAATGGCGTCAAGCTGACCTGGAGCGa    <  4:233733/135‑1 (MQ=255)
                                                                                                                               aTTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTACCTCAAGTACGTCAACGCCCAGGGCGGCGTGAATGGCGTCAAGCTGACCTGGAGCGAGt  <  2:121013/135‑1 (MQ=255)
                                                                                                                               aTTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTACCTCAAGTACGTCAACGCCCAGGGCGGCGTGAATGGCGTCAAGCTGACCTGGAGCGAGt  <  5:249942/135‑1 (MQ=255)
                                                                                                                               aTTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTACCTCAAGTACGTCAACGCCCAGGGCGGCGTGAATGGCGTCAAGCTGACCTGGAGCGAGt  <  4:243457/135‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                   
CTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACGTGCACGCGGCCAACGAACAGTATTTCCCCCTGCAGAGCTACCGCGTTGGCCCCTATGCCGCAGGTGGTACCGGTTTCTTCGGTGGTTTTATCGATTACCTCAAGTACGTCAACGCCCAGGGCGGCGTGAATGGCGTCAAGCTGACCTGGAGCGAGT  >  ACB122/3146815‑3147076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑