Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,302,084 G→A 9.8% G48S (GGC→AGC)  lhgD → L‑2‑hydroxyglutarate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,302,0840GA 9.8% 110.0 / 4.1 41G48S (GGC→AGC) lhgDL‑2‑hydroxyglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (20/17);  new base A (2/2);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-01

ATTTCATCATCATTGGCGGCGGGATTGTGGGCATGTCCACGGCCATGCACCTGATCAAGGTCTACCCGGACGCGAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCAGCCTCAAGGCGCGCTTCTGCCTGGAAGGCAACAAGGCGACCAAAGCCTTCTGCACCCAGCACGGCATTCGCTTCGATGAGTGCGGCAAGC  >  ACB122/3301950‑3302210
                                                                                                                                      |                                                                                                                              
aTTTCATCATCATTGGCGGCGGGATTGTGGGCATGTCCACGGCCATGCACCTGATCAAGGTCTACCCGGACGCGAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAgcg                                                                                                                                <  7:160781/135‑1 (MQ=255)
    catcatcatTGGCGGCGGGATTGTGGGCATGTCCACGGCCATGCACCTGATCAAGGTCTACCCGGACGCGAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAgcggcg                                                                                                                             >  8:40253/1‑134 (MQ=255)
    catcatcatTGGCGGCGGGATTGTGGGCATGTCCACGGCCATGCACCTGATCAAGGTCTACCCGGACGCGAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGt                                                                                                                            >  3:216647/1‑135 (MQ=255)
            ttGGCGGCGGGATTGTGGGCATGTCCACGGCCATGCACCTGATCAAGGTCTACCCGGACGCGAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCCCCATCAGACCGGCCATAACAGCGGCGTGATCCACg                                                                                                                    <  1:88256/135‑1 (MQ=255)
               ggggcggGATTGGGGGCCTGTCCACGGCCATGCACCTGATCATGGTCTACCCGGACGCGAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATCCACGcc                                                                                                                  <  6:99316/132‑1 (MQ=255)
                       tttGGGGGCAGGTCCACGGCCATGCACCTGATCAAGGTCTACCCGGACGCGAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATCCACGCCGGCGTGTAt                                                                                                         <  6:230082/134‑1 (MQ=255)
                               cATGTCCACGGCCATGCACCTGATCAAGGTCTACCCGGACGCGAAGATGATCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATCCACGCCGGCGTGTATTACAcccc                                                                                                 <  4:74543/135‑1 (MQ=255)
                                     cACGGCCATGCACCTGATCAAGGTCTACCCGGACGCGAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCAg                                                                                           >  4:46609/1‑135 (MQ=255)
                                        ggCCATGCACCTGATCAAGGTCTACCCGGACGCGAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCCGCCt                                                                                        >  6:269470/1‑135 (MQ=255)
                                            aTGCACCTGATCAAGGTCTACCCGGACGCGAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCAGCCTCAAg                                                                                    <  8:268067/135‑1 (MQ=255)
                                            aTGCACCGGATCAAGGTCTACCCGGACGCGAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCAGCCTCAAg                                                                                    <  4:30832/135‑1 (MQ=255)
                                                aCCTGATCAAGGTCTACCCGGACGCGAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATcc                                                                                                                       <  8:130483/96‑1 (MQ=255)
                                                aCCTGATCAAGGTCTACCCGGACGCGAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATcc                                                                                                                       >  7:130483/1‑96 (MQ=255)
                                                aCCTGATCAAGGTCTACCCGGACGCGAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCAACAGACCGGCCATAACACCGGCCTGATCCCCGCCCGCGTGTATTACACCCCCCGCCCCCCCAACgcgc                                                                                >  3:26948/1‑135 (MQ=255)
                                                       cAAGGTCTACCCGGACGCGAAGATGCTCCTGCTGTAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAgcg                                                                                                                                <  7:70042/80‑1 (MQ=255)
                                                       cAAGGTCTACCCGGACGCGAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCTGCCATAACAgcg                                                                                                                                >  8:70042/1‑80 (MQ=255)
                                                       cAAGGTCTACCCGGACGCGAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATCCACGcc                                                                                                                  <  1:251590/94‑1 (MQ=255)
                                                       cAAGGTCTACCCGGACGCGAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATCCACGcc                                                                                                                  >  2:251590/1‑94 (MQ=255)
                                                       cAAGGTCTACCCGGACGCGAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATAAGACCGGCCATAACAGCGGCGTGATCCACGCCGGCGTGTATTACACCCCCCGCCGCCTCAAGGCGCGCTTCTg                                                                         >  4:9780/1‑135 (MQ=255)
                                                        aaGGTCTACCCGGACGCGAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATCCACGCCGGCGTGTATTACAcccc                                                                                                 <  4:243291/110‑1 (MQ=255)
                                                        aaGGTCTACCCGGACGCGAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATCCACGCCGGCGTGTATTACAcccc                                                                                                 >  3:243291/1‑110 (MQ=255)
                                                             cTACCCGGACGCGAATATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGCCAGGCCATAACCGCGGCGTGATCCACGCCGGCGTGTAATACACCCCCCGCACCCTCCAGGCGCGCTTCTGCCTg                                                                     >  3:144079/1‑133 (MQ=255)
                                                                 ccGGACGCGAAGATGCTCCTGCGGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCAGCCTCAAGGCGCGCTTCTGCCTGGAAGGc                                                               >  4:69143/1‑135 (MQ=255)
                                                                  cGGACGCGAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCAGCCTCAAGGCGCGCGTCTGCCTGGAAGGca                                                              >  5:109641/1‑135 (MQ=255)
                                                                        cgAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCAGCAGACCGGCCATAACAGCGGCGGGATCCACGCCGGCGTGTATTACACCCCCCGCAGCCTCCAGGCCCGCTTCGTCCTGGACGGCAACCAgg                                                        >  4:232806/1‑135 (MQ=255)
                                                                           aGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCAGCCTCAAGGCGCGCTTCTGCCTGGAAGGCAACAAGGCGa                                                     <  7:62481/135‑1 (MQ=255)
                                                                           aGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCAGCCTCAAGGCGCGCTTCTGCCTGGAAGGCAACAAGGCGa                                                     <  8:3742/135‑1 (MQ=255)
                                                                           aGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCAGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCAGCCTCAAGGCGCGCTTCTGCCTGGAAGGCAACAAGGCGa                                                     >  2:38768/1‑135 (MQ=255)
                                                                           aGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCAGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCAGCCTCAAGGCGCGCTTCTGCCTGGAAGGCAACAAGGCGa                                                     >  2:33762/1‑135 (MQ=255)
                                                                             aTGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCAGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCAGCCTCAAGGCGCGCTTCTGCCTGGAAGGCAACAAGGCGAcc                                                   <  1:38768/135‑1 (MQ=255)
                                                                             aTGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCAGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCAGCCTCAAGGCGCGCTTCTGCCTGGAAGGCAACAAGGCGAcc                                                   <  1:33762/135‑1 (MQ=255)
                                                                             aTGCTCCTGCTGGAGAAAATGGCTGGCCCGGCTCGCCATCAGACCGTCATTAACAGCGGCTTGATCCAGGCGGGCGTGTATTAAACCCCCGGCAGCCTCATGGGGCGCTTCTGCCTGGAAGGCAACAAGGCGAcc                                                   <  2:257244/135‑1 (MQ=255)
                                                                                  ccTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCACCCTCAAGGCGCGCTTCTGCCAGGAAGGCAACAAGGCGACCAAAGc                                              >  7:15128/1‑135 (MQ=255)
                                                                                    tgctgGAGCAAGCGGCGGGCCCGGCGCGCCATCAGACCGGCCATAACAGGGGCGTGAGCCACGCCGGCGTGTATTACACCCCCGGCAGCCTCAAGGCGCGCTTCTGCCTGGAAGGCAACAAGGCGACCAAAGCCt                                            <  5:181387/135‑1 (MQ=255)
                                                                                       tgGAGAAAGAGGCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCAGCCTCAAGGCGCGCTTCTGCCTGGAAGGCAACAAGGCGACCAAAGCCTTCt                                         <  3:270177/135‑1 (MQ=255)
                                                                                                     ggCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCAGCCTCAAGGCGCGCTTCTGCGTGGAAGGCAACAAGGCGACCAAAGCCTTCTGCACCCAGCACgg                            <  2:217972/134‑1 (MQ=255)
                                                                                                             tCGCCATCAGACCGGCCATAACAGCGGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCAGCCTCAAGGCGCGCTTCTGCCTGGAAGGCAACAAGGGGACCAAAGCCTTCTGCACCCAGCACGGCATTCGCtt                   <  3:52677/135‑1 (MQ=255)
                                                                                                               gCCATCAGACCGGCCATAACAGCGGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCCGCCTCAAGGCGCGCTTCTGCCTGGAAGGCAACAAGGCGCCCAACGCCTTCTGCACCCCGCACGGCATGCGCTTCg                 >  6:268827/1‑135 (MQ=255)
                                                                                                               gCCATCAGACCGGCCATAACAGCGGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCAGCCTCAAGGCGCGCTTCTGCCTGGAAGGCAACCAGGCGACCAAAGCCTTCTGCACCCAGCACGGCATTCGCTTCg                 >  4:80852/1‑135 (MQ=255)
                                                                                                               gCCATCAGACCGGCCATAACAGCGGCGTGATCCACGCCCGCGTGTAATTCACCACCGGCAGCCTCAAGGCGCGCTTCTGCCTGTAAGGCAACAAGGGGACCAAAGCCATCTGCACCCACCACGGCATTCGCTTc                  >  1:217972/1‑134 (MQ=255)
                                                                                                                ccATCAGACCGGCCATAACAGCGGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCAGCCTCAAGGCGCGCTTCTGCCTGGAAGGCAACAAGGCGACCAAAGCCTTCTGCACCCAGCACGGCATTCGCTTc                  <  2:162633/133‑1 (MQ=255)
                                                                                                                              aTAACAGCGGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCAGCCTCAAGGCGCGCTTCTGCCTGGAAGGCAACAAGGCGACCAAAGCCTTCTGCACCCAGCACGGCATTCGCTTCGATGAGTGCGGCAAgc  >  7:39131/1‑135 (MQ=255)
                                                                                                                              aTAACAGCGGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCAGCCTCAAGGCGCGCTTCTGCCTGGAAGGCAACAAGGCGACCAAAGCCTTCTGCACCCACCACGGCATTCGCGTCGATGAGTGCGGCAAgc  >  3:238619/1‑135 (MQ=255)
                                                                                                                              aTAACAGCGGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCAGCCTCAAGGCGCGCTTCTGCCTGGAAGGCAACAAGGCGACCAAAGCCGTCTGCACCCAGCACGGCATTCGCTTCGATGAGTGCGGCAAgc  >  4:32484/1‑135 (MQ=255)
                                                                                                                              aTAACAGCGGCGTGATCCACGCCGGCGTGTATTAAACCACCGCCACCCTCAAGGCGCGCTTCAGCCTGGAATGCAACAAGGCGACCAAAGCCTTATGCACCCAGCACGGCATACGCTTCGATGACTGCGGCaacc  >  3:238318/1‑133 (MQ=255)
                                                                                                                                      |                                                                                                                              
ATTTCATCATCATTGGCGGCGGGATTGTGGGCATGTCCACGGCCATGCACCTGATCAAGGTCTACCCGGACGCGAAGATGCTCCTGCTGGAGAAAGAGTCTGGCCCGGCTCGCCATCAGACCGGCCATAACAGCGGCGTGATCCACGCCGGCGTGTATTACACCCCCGGCAGCCTCAAGGCGCGCTTCTGCCTGGAAGGCAACAAGGCGACCAAAGCCTTCTGCACCCAGCACGGCATTCGCTTCGATGAGTGCGGCAAGC  >  ACB122/3301950‑3302210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑