Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,308,718 A→C 29.7% R166R (CGT→CGG)  CJ019_03110 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,308,7180AC29.7% 39.3 / 2.4 34R166R (CGT→CGG) CJ019_03110hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (18/5);  new base C (2/9);  total (20/14)
Fisher's exact test for biased strand distribution p-value = 1.99e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCAC‑CAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTGGCCGCCAACAGCGCAGGG  >  ACB122/3308586‑3308847
                                                                                                                                     |                                                                                                                                 
cgcACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCAC‑CAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACg                                                                                                                                 <  2:279236/135‑1 (MQ=255)
        tGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACCTGCCGGCCCAAGGCAC‑CAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCACCGCCCCCGGc                                                                                                                         >  5:167095/1‑135 (MQ=255)
        tGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCAC‑CAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGc                                                                                                                         >  6:75926/1‑135 (MQ=255)
        tGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCAC‑CAACTGCACCCGCCAGCCGTAGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGc                                                                                                                         >  7:295873/1‑135 (MQ=255)
             gCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCACCATGCCGGCCCAAGGCAC‑CAACTGCACCCGCCCGCCGTTGCGCCGCTCGCCCCGCAGCCCCCGCCCCCGGCCGCgg                                                                                                                    >  1:238999/1‑135 (MQ=255)
             gCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCAC‑CAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCCGCAGCCAACGCCCCAGGCCGCgg                                                                                                                    >  3:30112/1‑135 (MQ=255)
             gCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCAC‑CAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCgg                                                                                                                    >  2:139147/1‑135 (MQ=255)
             gCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCAC‑CAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCgg                                                                                                                    >  7:175398/1‑135 (MQ=255)
                aGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCAGAGCGCGGCGCCCAGCAACAAGCCGGCCCAAGGCAC‑CAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCCACGCCCCAGGCCGCGccc                                                                                                                  >  2:66458/1‑134 (MQ=255)
                           gCCCAGGGGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCAC‑CAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAg                                                                                                      <  7:168835/135‑1 (MQ=255)
                                   gttgttGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAGGGCAC‑CAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAACCGCGACAGGGCGCCCg                                                                                              >  2:73772/1‑135 (MQ=255)
                                     gtttgCACCCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCAC‑CAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCAACCGCCCCAGGCCGGGGCCGAAACGCGACAGGGCGCCCGtt                                                                                            <  6:98832/133‑1 (MQ=255)
                                               aGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCAC‑CAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCCGCCAACGCCCCCGGCCGCGGCCGAACCGCGACAGGGCGCCCGTTACGTAAGTCa                                                                                  >  6:97201/1‑135 (MQ=255)
                                               aGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCAC‑CAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAACCGCGACAGGGCGCCCGTTACGTAAGTCa                                                                                  >  2:97787/1‑135 (MQ=255)
                                                   gcCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCAC‑CAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCCGGCCGCGGCCGAACCGCGACAGGGCGCCCGTTACGTAAGTCAGGcc                                                                              >  5:282782/1‑135 (MQ=255)
                                                                 ccAGCAACATGCCGGCCCAAGGCAC‑CAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCAt                                                                >  3:100777/1‑135 (MQ=255)
                                                                   agaaaCAGGCCGGCCCCGGCCAC‑CACCTCCACCCCCCCGCCGTGGCGCCGCCCGCCCAGCCCCCACCGCCCCCGGCCGCGGCCGAACCGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTc                                                              <  1:73772/132‑1 (MQ=255)
                                                                             cGGCCCAAGGCAC‑CAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCa                                                    >  3:144317/1‑135 (MQ=255)
                                                                              ggCCCAAGGCAC‑CAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCa                                                    >  3:106894/1‑134 (MQ=255)
                                                                              cggccAACGCACACAACTCCACCCGCCCGCCGTGGCGCGGCTCCCCCCGCCGCAACCGCCCCCGGGCGCGGCCTAACCGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCa                                                    <  2:223866/132‑1 (MQ=255)
                                                                                  catgtcAC‑CTACTCCACCCGCCCGCGGTTGCGCCGCTGCCCCACAACCCAACGCCCCCGGCGGCGTCAGAACCGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGAcc                                               <  4:95471/130‑1 (MQ=255)
                                                                                         c‑cACCTGCACCCGCCAGCCGGGGCGCCGCTCGCCCCGCAGCCACCGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGa                                        <  8:195179/135‑1 (MQ=255)
                                                                                         c‑cAACTCCACCCGCCCCCCGTGGCGCCGCTCCCCCCCCACCCACCGCCCCCGGCCGCGGCCTAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGa                                        <  1:129979/135‑1 (MQ=255)
                                                                                              cTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCACCCACCGCCCCCGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCAt                                    <  8:175398/135‑1 (MQ=255)
                                                                                                        cAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATgg                            >  6:216379/1‑133 (MQ=255)
                                                                                                         aGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAACCGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCCCGCCCGCAGTGATCATGCCCATGGCCa                         >  5:198078/1‑135 (MQ=255)
                                                                                                         aGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCa                         >  3:278199/1‑135 (MQ=255)
                                                                                                         aGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCa                         >  1:10402/1‑135 (MQ=255)
                                                                                                         aGCCGTTGCCCCGCGCGCCCAGCAGCCAACGCCCCAGCCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCa                         <  4:286335/135‑1 (MQ=255)
                                                                                                              ttGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTg                    >  3:131269/1‑135 (MQ=255)
                                                                                                                   ccgcccgcCCCCCACCCACCCCCCCCGCCCGCGGCCGAACCGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTGgccgc               <  5:75926/135‑1 (MQ=255)
                                                                                                                   ccgcGCGCCCCCCCGCCACCGCCCCAGCCCGCGGCCGAACCGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTGgccgc               <  7:28967/135‑1 (MQ=255)
                                                                                                                          cccAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTGGCCGCCAACa          <  4:144035/133‑1 (MQ=255)
                                                                                                                                ccccACCGCCCCCGCCCGCGCCCCAACCGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTGGCCGCCAACAGCGCAggg  <  5:214523/133‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                 
CGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCAC‑CAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTGGCCGCCAACAGCGCAGGG  >  ACB122/3308586‑3308847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑