Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	3,427,284	G→A	7.7%	V127I (GTC→ATC)	CJ019_03250 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	3,427,284	0	G	A	7.7%	160.3 / 3.0	52	V127I (GTC→ATC)	CJ019_03250	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/20);  new base A (2/2);  total (30/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGGCTGTACGGCAATGCCGGCCCGCAGCCGGACAACCGCGACCATGTCGAGGTGTGGCGATTCGATGATGGCGGCTCACTGGTCTACGACTGGGAAGCCAATAGCTACACCATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAGGCGCCGTCGATCACCCTGGAGGGTAAC  >  ACB122/3427150‑3427412                                                                                                                                       |                                                                                                                                  cGGGGCTGTACGGCAATGCCGGCCCGCAGCCGGACAACCGCGTCCATGTCGAGGTGTGGCGATTCGATGATGGCGGCTCACTGGTCTACGACTGGGAAGCCAATAGCTACACCATCAAGCTGCCCACGGGCACgg                                                                                                                                   >  4:181710/1‑135 (MQ=255) cGGGGCTGTACGGCAATGCCGGCCCGCAGCCGGACAACCGCGACCATGTCGAGGTGTGGCGATTCGATGATGGCGGCTCACTGGTCTACGACTGGGAAGCCAATAGCTACACCATCAAGCTGCCCACGGGCACgg                                                                                                                                   >  3:186546/1‑135 (MQ=255)      cTGTACGGCAATGCCGGCCCGCAGCCGGACAACCGCGACCATGTCGAGGTGTGGCGATTCGATGATGGCGGCTCACTGGTCTACGACTGGGAAGCCAATAGCTACACCATCAAGCTGCCCACGGGCACGGTCAcc                                                                                                                              <  4:168945/135‑1 (MQ=255)      cTGTACGGCAATGCCGGCCCGCAGCCGGACAACCGCGACCATGTCGAGGTGTGGCGATTCGATGATGGCGGCTCACTGGTCTACGACTGGGAAGCCAATAGCTACACCATCAAGCTGCCCACGGGCACGGTCAcc                                                                                                                              >  8:127397/1‑135 (MQ=255)           cGGCAATGCCGGCCCGCAGCCGGACAACCGCGACCATGTCGAGGTGTGGCGATTCGATGATGGCGGCTCACTGGTCTACGACTGGGAAGCCAATAGCTACACCATCAAGCTGCCCACGGGCACGGTCACCATTGa                                                                                                                         >  1:209984/1‑135 (MQ=255)                   ccGGCCCGCAGCCGGACAACCGCGACCATGTCGAGGTGTGGCGATTCGATGATGGCGGCTCACTGGTCTACGACTGGGAAGCCAATAGCTACACCATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCggtg                                                                                                                 >  8:106866/1‑135 (MQ=255)                    cGGCCCGCAGCCGGACAACCGCGACCATGTCGAGGTGTGGCGATTCGATGATGGCGGCTCACTGGTCTACGACTGGGAAGCCAATAGCTACACCATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCggtg                                                                                                                 >  4:56885/1‑134 (MQ=255)                      gCCCGCAGCCGGACAACCGCGACCATGTCGAGGTGTGGCGATTCGATGATGGCGGCTCACTGGTCTACGACTGNGAAGCCAATAGCTACACCATCAAGCTGCCCACGGGCACGATCACCATTGAGGTCGGTGGTa                                                                                                              >  5:214357/1‑135 (MQ=255)                      gCCCGCAGCCGGACAACCGCGACCATGTCGAGGTGTGGCGATTCGATGATGGCGGCTCACTGGTCTACGACTGGGAAGCCAATAGCTACACCATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTa                                                                                                              >  2:121232/1‑135 (MQ=255)                      gCCCGCAGCCGGACAACCGCGACCATGTCGAGGTGTGGCGATTCGATGATGGCGGCTCACTGGTCTACGACTGGGAAGCCAATAGCTACACCATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTa                                                                                                              >  2:210243/1‑135 (MQ=255)                      gCCCGCAGCCGGACAACCGCGACCATGTCGAGGTGTGGCGATTCGATGATGGCGGCTCACTGGTCTACGACTGGGAAGCCAATAGCTACACCATCAAGCTGCCCACGGGCACGATCACCATTGAGGTCGGTGGTa                                                                                                              >  5:210390/1‑135 (MQ=255)                             gCCGGACAACCGCGACCATGTCGAGGTGTGGCGATTCGATGATGGCGGCTCACTGGTCTACGACTGGGAAGCCAATAGCTACACCATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑c                                                                                                      <  7:1118/135‑1 (MQ=255)                                         cgACCATGTCGAGGTGTGGCGATTCGATGATGGCGGCTCACTGGTCTACGACTGGGAAGCCAATAGCTACACCATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTga                                                                                          >  2:192573/1‑135 (MQ=255)                                               tGTCGAGGTGTGGCGATTCGATGATGGCGGCTCACTGGTCTACGACTGGGAAGCCAATAGCTACACCATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGacac                                                                                    <  1:145694/135‑1 (MQ=255)                                                             gATTCGATGATGGCGGCTCACTGGTCTACGACTGGGAAGCCAATAGCTACACCATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGa                                                                      >  5:217832/1‑135 (MQ=255)                                                               ttCGTTGATGGCGGCTCACTGGTCTACTACTGGGAACCCAATAGCTACACCATCACGCTGCCCACGGGCACGGTCACAATTGAGGTGGGTGGTAGCAAGG‑CTGTCATCACTGACGACACTATCAGCGCCAAGAcc                                                                    <  1:210243/135‑1 (MQ=255)                                                               tgcGATGATGGCGGCTCACTGGTCTACGACTGGGAAGCCAATAGCAACACCATCAAGCGGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGAcc                                                                    <  7:290593/133‑1 (MQ=255)                                                                  gaggatgGCGGCGCACTGGTCTACGACTGGGACGCCAATAGCTACACCATCAAGCTGCCCCCGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCac                                                                  <  5:54123/131‑1 (MQ=255)                                                                               cACTGGTCTACGACTGGGAAGCCAATAGCTACACCATCAAGCTCCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAAc                                                      >  8:177188/1‑133 (MQ=255)                                                                                           aCTGGGAAGCCAATAGCTACACCATCACGCGGCCCCCGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGgccg                                        <  3:56885/135‑1 (MQ=255)                                                                                            cggggAAGCCAATAGCTACACCATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAACACCACGACGATGACAACCGAGGCGCAGgccgc                                       <  4:130330/133‑1 (MQ=255)                                                                                                 aaGCCAATAGCTACACCATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACgg                                  >  2:5390/1‑135 (MQ=255)                                                                                                 aaGCCAATAGCTACACCATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACgg                                  >  8:297201/1‑135 (MQ=255)                                                                                                  aGCCAATAGCTACACCATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGCCGATGACAACCGAGGCGCAGGCCGCCACgg                                  >  8:84366/1‑134 (MQ=255)                                                                                                  aGCCAATAGCTACACCATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACgg                                  >  8:293222/1‑134 (MQ=255)                                                                                                  aGCCAATAGCTACACCATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACgg                                  >  5:191940/1‑134 (MQ=255)                                                                                                  aGCCAATAGCTACACCATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGt                                 >  6:153752/1‑135 (MQ=255)                                                                                                      aatacatACACCATCAAGCTCCCCCCGGGCAGGGGCACCATGGCGGGCGGTGGTAGAAAGG‑CAGCCAGCACTGACTACCCGAGCAGCCCCACGCCCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAg                             <  4:186546/129‑1 (MQ=255)                                                                                                      aaTAGCTACACCATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAg                             <  7:164232/135‑1 (MQ=255)                                                                                                      aaTAGCTACACCATCAAGCTGCCCACGGGCACGGTCACCATTGAGGGCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAg                             <  7:38159/135‑1 (MQ=255)                                                                                                         aGCTACACCATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAGgcg                          >  4:83740/1‑135 (MQ=255)                                                                                                              cacCATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAGGCGCCGTc                     <  6:79165/135‑1 (MQ=255)                                                                                                               acCATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTGGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAGGCGCCGTc                     <  6:191940/134‑1 (MQ=255)                                                                                                                ccATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGGCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAGGCGCCGTCGa                   <  5:104383/135‑1 (MQ=255)                                                                                                                 cATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAGGCGCCGTCGAt                  >  1:94690/1‑135 (MQ=255)                                                                                                                 cATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGAAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAACGCGCCGTCGa                   >  1:277184/1‑134 (MQ=255)                                                                                                                  aTCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAGGCGCCGTCGATc                 >  8:225940/1‑135 (MQ=255)                                                                                                                      aGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGCCCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAGGCGCCGTCGATCAccc             >  6:282783/1‑135 (MQ=255)                                                                                                                      aGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAGGCGCCGTCGATCAccc             >  1:209169/1‑135 (MQ=255)                                                                                                                      aGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAGGCGCCGTCGATCAccc             >  1:7799/1‑135 (MQ=255)                                                                                                                      aGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAGGCGCCGTCGATCAcc              <  3:52511/134‑1 (MQ=255)                                                                                                                      aGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGAGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAGGCGCCGTCGATCAccc             >  8:71167/1‑135 (MQ=255)                                                                                                                       gcggCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAGGCGCCGTCGATCACCCt            <  4:158919/132‑1 (MQ=255)                                                                                                                       gCTGCCCCCGGGCACGATCACCATTGCGGGCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAGGCGCCGTCGATCACCCt            <  6:214357/135‑1 (MQ=255)                                                                                                                       gCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAGGCGCCGTCGATCACCCt            >  1:28558/1‑135 (MQ=255)                                                                                                                       gCTGCCCACGGGCACGATCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAGGCGCCGTCGATCACCCt            <  6:210390/135‑1 (MQ=255)                                                                                                                       ccgcccccCGGGACCGGCCACCATGGAGGGCGGGGGT‑CCAAGGCCAGTCATCACTGCCCACCCGAGCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAGGCGCCGTCGATCACCCt            <  1:148780/131‑1 (MQ=255)                                                                                                                        cTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAGGCGCCGTCGATCAccat            >  1:211414/1‑132 (MQ=255)                                                                                                                            ccACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAGGCGCCGTCGATCACCCTGGAgg       <  1:192573/135‑1 (MQ=255)                                                                                                                              aCGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTgac                                                                                         >  8:241461/1‑51 (MQ=255)                                                                                                                              aCGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTgac                                                                                         <  7:241461/51‑1 (MQ=255)                                                                                                                              aCGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCACGGCGCCGTCGATCCCCCTGGAggg      >  5:171578/1‑134 (MQ=255)                                                                                                                              aCGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAGGCGCCGTCGATCACCCTGGAggg      >  8:204222/1‑134 (MQ=255)                                                                                                                                 ggCACGGTCACCATTGCGGGCGGTTGTAGCAAGC‑CATTCATCACTGACGACACGATCCGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAGGCGCCGTCGATCACCCTGGAGGGTAAc  <  7:131435/135‑1 (MQ=255)                                                                                                                                       |                                                                                                                                  CGGGGCTGTACGGCAATGCCGGCCCGCAGCCGGACAACCGCGACCATGTCGAGGTGTGGCGATTCGATGATGGCGGCTCACTGGTCTACGACTGGGAAGCCAATAGCTACACCATCAAGCTGCCCACGGGCACGGTCACCATTGAGGTCGGTGGTAGCAAGG‑CAGTCATCACTGACGACACGATCAGCGCCAAGACCACGACGATGACAACCGAGGCGCAGGCCGCCACGGTCAAGGCGCCGTCGATCACCCTGGAGGGTAAC  >  ACB122/3427150‑3427412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑