| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 3,671,624 | C→A | 14.3% | intergenic (+1/+42) | CJ019_03482 → / ← CJ019_03483 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,671,624 | 0 | C | A | 14.3% | 58.0 / 5.7 | 28 | intergenic (+1/+42) | CJ019_03482/CJ019_03483 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (12/12); new base A (2/2); total (14/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.34e-01 | |||||||||||
GACCTGGCGCGGGAGGGGGGCACTATCGAGGCGCTGGTCGCGGCGGCTGATGGGGCGTTGTACCAGGCCAAACAGCAGGGGCGAGATGGGTATTGCGTGGCCGGAAGTTTGGCGGCAGGGCAGAATTGCTGACCTGGCTGGAGATCGCGGGGGCGCCATGCGGCGCGATGAAAACTAGAGCCCCACGTTCAGACGCATTGCACAACGCCCGAGAAGACCGTGAGCATGCTCGCTGGCCAGGATGCCACGGAGTCGTTCGCAAAGC > ACB122/3671492‑3671756 | gACCTGGCGCGGGAGGGGGGCACTATCGAGGCGCTGGTCGCGGCGGCTGATGGGGCGTTGTACCAGGCCAAACAGCAGGGGCGAGATGGGTATTGCGTGGCCGGAAGTTTGGCGGCAGGGCAGAATTGCTGACct > 2:94851/1‑135 (MQ=255) ggggggCACTATCGAGGCGCTGGTCGCGGCGGCTGATGGGGCGTTGTACCAGGCCAAACAGCAGGGGCGAGATGGGTATTGCGTGGCCGGAAGTTTGGCGGCAGGGCAGAATTGCTGACCTGGCTGGAGATcgcg < 7:255757/135‑1 (MQ=255) cACTATCGAGGCGCTGGTCGCGGCGGCTGATGGGGCGTTGTACCAGGCCAAACAGCAGGGGCGAGATGGGTATTGCGTGGCCGGAAGTTTGGCGGCAGGGCAGAATTGCTGACCTGGCTGGAGATCGCGGGGgcg > 8:201782/1‑135 (MQ=255) cgcTGGTCGCGGCGGCTGATGGGGCGTTGTACCAGGCCAAACAGCAGGGGCGCGATGGGTATTGCGTGGCCGGAAGTTTGGCGGCAGGGCAGAATTGCTGACCTGGCTGGAGATCGCGGGGGCGCCATggggcgg > 5:279287/1‑134 (MQ=255) gTCGCGGCGGCTGATGGGGCGTTTTAACAGGCCAAACAGCAGGTGAGAGATGGGTATTGCGTGGCCGCAAGTTTGGCGGCAGGGCAGAATTGCTGACCTTGCTGGAGATCGCGGGGGCGCCCTGCGGCGCGATg > 6:73299/1‑134 (MQ=255) gcggcgGCTGATGGGGCGTTGTACCAGGCCAAACAGCAGGGGCGAGATGGGTATTGCGTGGCCGGAAGTTTGGCGGCAGGGCAGAATTGCTGACCTGGCTGGAGATCGCGGGGGCGCCATGCGGCGCGATGaaaa < 8:182975/135‑1 (MQ=255) gCGTTGTACCAGGCCAAACAGCATGGGCGAGATGGGTATTGCGTGGCCGGAAGTTTGGCGGCAGGGCAGAATTGCTGACCTGGCTGGAGATCGCGGGGGCGCCATGCGGCGCGATGAAAACTAGAGCCCCACGtt < 5:136512/135‑1 (MQ=255) gCGTTGTACCAGGCCAAACAGCAGGGGCGAGATGGGTATTGCGTGGCCGGAAGTTTGGCGGCAGGGCAGAATTGCTGACCTGGCTGGAGATCGCGGGGGCGCCATGCGGCGCGATGAAAACTAGAGCCCCACGtt < 1:94851/135‑1 (MQ=255) ttGTACCAGGCCAAACAGCAGGGGCGAGATGGGTATTGCGTGGCCGGAAGTTTGGCGGCAGGGCAGAATTGCTGACCTGGCTGGAGATCGCGGGGGCGCCATGCGGCGCGATGAAAACTAGAGCCCCACGTTCAg < 7:20410/135‑1 (MQ=255) ccAAACAGCAGGGGCGAGATGGGTATTGCGTGGCCGGAAGTTTGGCGGCAGGGCAGAATTGCTGACCTGGCTGGAGATCGCGGGGGCGCCATGCGGCGCGATGAAAACTAGAGCCCCACGTTCAGACGCATTGca < 7:201782/135‑1 (MQ=255) cAAACAGCAGGGGCGAGATGGGTATTGCGTGGCCGGAAGTTTGGCGGCAGGGCAGAATTGCTGACCTGGCTGGAGATCGCGGGGGCGCCATGCGGCGCGATGAAAACTAGAGcc < 1:191622/114‑1 (MQ=255) cAAACAGCAGGGGCGAGATGGGTATTGCGTGGCCGGAAGTTTGGCGGCAGGGCAGAATTGCTGACCTGGCTGGAGATCGCGGGGGCGCCATGCGGCGCGATGAAAACTAGAGcc > 2:191622/1‑114 (MQ=255) aCAGCAGGGGCGAGATGGGTATTGCGTGGCCGGAAGTTTGGCGGCAGGGCAGAATTGCTGACCTGGCTGGAGATCGCGGGGGCGCCATGCGGCGCGATGAAAACTAGAGCCCCACGTTCAGACGCATTGCACAAc < 4:162174/135‑1 (MQ=255) agcagGGGCGAGATGGGTATTGCGTGGCCGGAAGTTTGGCGGCAGGGCAGAATTGCTGACCTGGCTGGAGATCGCGGGGGCGCCATGCGGCGCGATGAAAACTAGAGCCCCACGTTCAGACGCATTGca > 2:253620/1‑129 (MQ=255) agcagGGGCGAGATGGGTATTGCGTGGCCGGAAGTTTGGCGGCAGGGCAGAATTGCTGACCTGGCTGGAGATCGCGGGGGCGCCATGCGGCGCGATGAAAACTAGAGCCCCACGTTCAGACGCATTGca < 1:253620/129‑1 (MQ=255) agcagGGGCGAGATGGGTATTGCGTGGCCGGAAGTTTGGCGGCAGGGCAGAATTCCTGACCTGGCTGGAGATCGCGGGGGCGCCATGCGGCGCGATGAAAACTAGAGCCCCACGTTCAGACGCATTGCACACCGc > 8:214291/1‑135 (MQ=255) ggggCGATATGGGTTTTGCGTGGCCGGAAGTTTGGCGGCAGTGCTGAATTGCTGACCTGTCTGGAGATCGCGGGGGCGCCTTGCGGCTCGATTAAAACTAGAGACCCACGTTCAGACGCATTGCACAACGccc < 7:214291/133‑1 (MQ=255) cGAGATGGGTATTGCGTGGCCGGAAGTTTGGCGGCAGGGCAGAATTGCTGACCTGGCTGGAGATCGCGGGGGCGCCATGCGGCGCGGTGAAAACATTAGGCCCCCGTTCAGCCGCCATGCCCCACGccccgggag > 5:164917/1‑129 (MQ=255) ggTATTGCGTGGCCGGAAGTTTGGCGGCAGGGCAGAATTGCTGACCTGGCTGGAGATCGCGGGGGCGCCATGCGGGGCGATGAAAACTAGAGCCCCACGTTCAGCCGCATTGCACAACGCCCGAGAAGACCGTGa > 3:205569/1‑135 (MQ=255) tGCGTGGCCGGAAGTTTGGCGGCAGGGCAGAATTGCTGACCTGGCTGGAGATCGCGGGGGCGCCATGCGGCGCGATGAAAACTAGAGCACCCCGTTCAGACGCATTGCCCAACGCCCGAGAAGCCCGTGAGCATg > 4:263444/1‑135 (MQ=255) gCCGGAAGTTTGGCGGCAGGGCAGAATTGCTGACCTGGCTGGAGATCGCGGGGGCGCCATGCGGCGCGATGAAAACTAGAGCCCCACGTTCAGACGCATTGCACAACGCCCGAGAAGACCGTGAGCATGCTCGCt < 3:134091/135‑1 (MQ=255) ccGGAAGTTTGGCGGCAGGGCAGAATTGCTGACCTGGCTGGAGATCGCGGGGGCGCCATGCGGCGCGATGAAAACTAGAGCCCCACGTTCAGACGCATTGCACAACGCCCGAGAAGACCGTGAGCATGCTCGCTg > 8:292869/1‑135 (MQ=255) cGGAAGTTTGGCGGCAGGGCAGAATTGCTGAACTGGCTGGAGATCGCGGGGGCGCCATGCGGCGCGATGAAAACTAGAGCCCCACGtt > 8:117345/1‑88 (MQ=255) cGGAAGTTTGGCGGCAGGGCAGAATTGCTGAACTGGCTGGAGATCGCGGGGGCGCCATGCGGCGCGATGAAAACTAGAGCCCCACGtt > 8:120949/1‑88 (MQ=255) cGGAAGTTTGGCGGCAGGGCAGAATTGCTGAACTGGCTGGAGATCGCGGGGGCGCCATGCGGCGCGATGAAAACTAGAGCCCCACGtt < 7:120949/88‑1 (MQ=255) cGGAAGTTTGGCGGCAGGGCAGAATTGCTGAACTGGCTGGAGATCGCGGGGGCGCCATGCGGCGCGATGAAAACTAGAGCCCCACGtt < 7:117345/88‑1 (MQ=255) ggcagggcagAATTGCTGACCTGGCTGGAGATCGCGGGGGCGCCATGCGGCGCGATGAAAACTAGAGCCCCACGTTAAGACGCATTGCACAACGCCCGAGAAGACCGTGAGCATGCTCGCTGGCCAGGATGCCAc > 3:150794/1‑135 (MQ=255) gACCTGGCGGGAGATCGCGGGGGCGCCATGCGGCGCGATGAAAACTAGAGCCCCACGTTCAGACGCATTGCACAACGCCCGAGTAGACCGTGAGCATGCTCGCTGGCGAGGATGCCACGGAGTCGTTCGCAAAGc < 6:300831/135‑1 (MQ=255) | GACCTGGCGCGGGAGGGGGGCACTATCGAGGCGCTGGTCGCGGCGGCTGATGGGGCGTTGTACCAGGCCAAACAGCAGGGGCGAGATGGGTATTGCGTGGCCGGAAGTTTGGCGGCAGGGCAGAATTGCTGACCTGGCTGGAGATCGCGGGGGCGCCATGCGGCGCGATGAAAACTAGAGCCCCACGTTCAGACGCATTGCACAACGCCCGAGAAGACCGTGAGCATGCTCGCTGGCCAGGATGCCACGGAGTCGTTCGCAAAGC > ACB122/3671492‑3671756 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |