Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,728,717 A→G 8.7% Q186R (CAG→CGG)  cnrA → Nickel and cobalt resistance protein CnrA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,728,7170AG 8.7% 138.1 / 4.2 46Q186R (CAG→CGG) cnrANickel and cobalt resistance protein CnrA
Reads supporting (aligned to +/- strand):  ref base A (18/24);  new base G (2/2);  total (20/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

CGTGACATTCGCGGTCTACGCGCTCAAAGCCAAAGGCGAACCGCAGCGCCAGTTGGTACGCGACGCCGAAACACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCTTGACAACCAGAACGCCCTGTCACCGTCGGGCTCGGTCGAAACGCAGGGGCCA  >  ACB122/3728586‑3728850
                                                                                                                                   |                                                                                                                                     
cgggACATCCGCGGAGTACGGGCTCAAAGCAAACGGCGAACCGCAGCGCGAGTTGGAACGCGACGCCGAAACACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCACCGGGGAACAGGc                                                                                                                                    <  1:127144/132‑1 (MQ=255)
cGTGTCATTCGGGGTCAACGCGCTCAAAGCAAACGGCGAAGCGCAGCGCAAGTTGGTACGCGACGCCGAAACACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGc                                                                                                                                    <  2:300691/135‑1 (MQ=255)
cGTGACATTCGCGGTCTACGCGCTCAAAGCCAAAGGCGAACCGCAGCGCCAGTTGGTACGCGACGCCGAAACACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGc                                                                                                                                    <  2:186670/135‑1 (MQ=255)
cGTGACATTCGCGGTCTACGCGCTCAAAGCCAAAGGCGAACCGCAGCGCCAGTTGGTACGCGACGCCGAAACACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATAATCGGGGAACAGGc                                                                                                                                    >  7:293035/1‑135 (MQ=255)
     cATTCGCGGTCTACGCGCTCAAAGCCAAAGGCGAACCGCAGCGCCAGTTGGTACGCGACGCCGAAACACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGCAc                                                                                                                               >  1:300691/1‑135 (MQ=255)
     cATTCGCGGTCTACGCGCTCAAAGCCAAAGGCGAACCGCAGCGCCAGTTGGTACGCGACGCCGAAACACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAAc                                                                                                                               <  1:198364/135‑1 (MQ=255)
     cATTCGCGGTCTACGCGCTCAAAGCCAAAGGCGAACCGCAGCGCCAGTTGGTACGCGACGCCGAAACACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAAc                                                                                                                               <  8:54346/135‑1 (MQ=255)
            ggTCTACGCGCTCAAAGCCAAAGGCGAACCGCAGCGCCAGTTGGTACGCGACGCCGAAACACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATAtt                                                                                                                        <  1:216757/135‑1 (MQ=255)
                           aGCCAAAGGCGAACCGCAGCGCCAGTTGGTACTCGACGCCGAAACACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAAAGGCTATTAGTGTCCTTTTCCCa                                                                                                         >  7:161858/1‑135 (MQ=255)
                           aGCCAAAGGCGAACCGCAGCGCCAGTTGGTACGCGACGCCGAAACACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTcc                                                                                                                 >  2:70128/1‑127 (MQ=255)
                           aGCCAAAGGCGAACCGCAGCGCCAGTTGGTACGCGACGCCGAAACACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTcc                                                                                                                 <  1:70128/127‑1 (MQ=255)
                                    cGAACCGCAGCGCCAGTTGGTACGCGACGCCGAAACACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCt                                                                                                >  7:136849/1‑135 (MQ=255)
                                     gAACCGCAGCGCCAGTTGGTACGCGACGCCGAAACACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTg                                                                                               <  2:167692/135‑1 (MQ=255)
                                      aaCCGCAGCGCCAGTTGGTACGCGACGCCGAAACACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTgg                                                                                              <  5:226022/135‑1 (MQ=255)
                                         cGCAGCGCCAGTTGGTACGCGACGCCGAAACACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCa                                                                                           >  1:260169/1‑135 (MQ=255)
                                         cGCAGCGCCAGTTGGTACGCGACGCCGAAACACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCa                                                                                           >  6:152561/1‑135 (MQ=255)
                                          gCAGCGCCAGTTGGTACGCGACGCCGAAACACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCACCATCATCGGGGCACAGTCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCat                                                                                          >  1:62827/1‑134 (MQ=255)
                                                ccAGTTGGTACGCGACGCCGAAACACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCg                                                                                     >  1:38576/1‑134 (MQ=255)
                                                             gACGCCGAAACACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGCATCACGCCACAg                                                                       >  1:202826/1‑135 (MQ=255)
                                                                    aaaCACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACAttt                                                                <  1:139682/135‑1 (MQ=255)
                                                                    aaaCACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACAttt                                                                <  2:130355/135‑1 (MQ=255)
                                                                        acTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTtctc                                                            <  2:237602/135‑1 (MQ=255)
                                                                             gcCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGccc                                                       >  5:194921/1‑135 (MQ=255)
                                                                             gcCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGcc                                                        <  7:256192/134‑1 (MQ=255)
                                                                              ccAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACGGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCt                                                      >  3:7752/1‑135 (MQ=255)
                                                                              ccAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACGGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCt                                                      >  5:71069/1‑135 (MQ=255)
                                                                               cagcagTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCtt                                                     >  5:184023/1‑135 (MQ=255)
                                                                               cagcagTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCt                                                      >  8:23946/1‑134 (MQ=255)
                                                                                        cttcACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGATCAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCTTGACAACCa                                             <  6:156970/131‑1 (MQ=255)
                                                                                        ctgcACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCTTGACAACCa                                             <  1:255185/134‑1 (MQ=255)
                                                                                        ctgcACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCTTGACAACCa                                             <  5:124114/134‑1 (MQ=255)
                                                                                        ctgcACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCTTGACAACCa                                             <  6:294869/134‑1 (MQ=255)
                                                                                            aCGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCTTGACAACCAGAAc                                         <  7:18389/134‑1 (MQ=255)
                                                                                                   gggCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCTTGACAACCAGAAc                                         >  1:176331/1‑127 (MQ=255)
                                                                                                   gggCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCTTGACAACCAGAAc                                         <  2:176331/127‑1 (MQ=255)
                                                                                                        tCAAGAAGGTCAACATCATCGGGGAACACGCTGAACCGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGCACATTTTCTCGGCCCTTGACAACCAGAACGCCCTGTCACCGt                            >  4:47417/1‑135 (MQ=255)
                                                                                                                      atcatcGGGGAACGGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCTTGACAACCAGAACGCCCTGTCACCGTCGGGCTCGGTCGaa              <  6:71069/135‑1 (MQ=255)
                                                                                                                      atcatcGGGGAACGGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCTTGACAACCAGAACGCCCTGTCACCGTCGGGCTCGGTCGaa              <  4:7752/135‑1 (MQ=255)
                                                                                                                        catcGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCTTGACAACCAGAACGCCCTGTCACCGTCGGGCTCGGTCGAAAc            <  6:184023/135‑1 (MQ=255)
                                                                                                                        catcGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCTTGACAACCAGAACGCCCTGTCACCGTCGGGCTCGGTCGAAAc            <  7:212909/135‑1 (MQ=255)
                                                                                                                        catcGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCTTGACAACCAGAACGCCCTGTCACCGTCGGGCTCGGTCGAAAc            <  7:23946/135‑1 (MQ=255)
                                                                                                                        catcGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCGGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCTTGACAACCAGAACGCCCTGTCACCGTCGGGCTCGGTCGAAAc            <  6:194921/135‑1 (MQ=255)
                                                                                                                             gggAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCTTGACAACCAGAACGCCCTGTCACCGTCGGGCTCGGTCGa               <  4:91761/127‑1 (MQ=255)
                                                                                                                             gggAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCTTGACAACCAGAACGCCCTGTCACCGTCGGGCTCGGTCGa               >  3:91761/1‑127 (MQ=255)
                                                                                                                             gggAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCTTGACAACCAGAACGCCCTGTCACCGTCGGGCTCGGTCGAAACGCAgg       >  1:261397/1‑135 (MQ=255)
                                                                                                                                aaCAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCTTGACAACCAGAACGCCCTGTCACCGTCGGGCTCGGTCGAAACGCAGGGGc    >  2:245025/1‑135 (MQ=255)
                                                                                                                                   aGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCTTGACAACCAGAACGCCCTGTCACCGTCGGGCTCGGTCGAAACGCAGGGGCca  >  2:112156/1‑134 (MQ=255)
                                                                                                                                   aGGCTGAACGGATATTCGTGTCATTTTACCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCTGCCCTTGACAACCAGAACGCCCTGTCACCGTCGGGCTCGGTCGAAACGCAGGGGCca  >  7:99517/1‑134 (MQ=255)
                                                                                                                                   |                                                                                                                                     
CGTGACATTCGCGGTCTACGCGCTCAAAGCCAAAGGCGAACCGCAGCGCCAGTTGGTACGCGACGCCGAAACACTGCGCCAGCAGTTGCTGCACGTACCGGGCGTCAAGAAGGTCAACATCATCGGGGAACAGGCTGAACGGATATTCGTGTCCTTTTCCCATGACCGCCTGGCCACGCTCGGAATCACGCCACAGGACATTTTCTCGGCCCTTGACAACCAGAACGCCCTGTCACCGTCGGGCTCGGTCGAAACGCAGGGGCCA  >  ACB122/3728586‑3728850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑