Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,841,593 G→A 7.5% P346P (CCG→CCA)  CJ019_03638 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,841,5930GA 7.5% 172.8 / 2.6 53P346P (CCG→CCA) CJ019_03638hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (20/29);  new base A (2/2);  total (22/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCAGACCCCCAACCTGAGCCTCACTGAGGCCAACGGCACCGGCGCCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCACGCAGGCCCAGTACCGCCTGTCCACCGGCGAGCTGACCCTGGTGGCCAGCACCAGTGACGAAACAACACCTCCAGTAC  >  ACB122/3841468‑3841723
                                                                                                                             |                                                                                                                                  
agccagACCCCCAACCTGAGCCTCACTGAGGCCAACGGCACCGGCGCCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACTCTGTCCGCTGACAACAGCGTGGCAATCCCGACCagcagc                                                                                                                           >  8:19859/1‑135 (MQ=255)
agccagACCCCCAACCTGAGCCTCACTGAGGCCAACGGCACCGGCGCCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACCCTGTCCGCTGACAACAGCGTGGCAATCCCGACCagcagc                                                                                                                           >  7:227682/1‑135 (MQ=255)
agccagACCCCCAACCTGAGCCTCACTGAGGCCAACGGCACCGGCGCCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGCCCagcagc                                                                                                                           >  3:188595/1‑135 (MQ=255)
     gACCCCCAACCTGAGCCTCACTGAGGCCAACGGCACCGGCGCCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGac                                                                                                                      >  4:275747/1‑135 (MQ=255)
     gACCCCCAACCTGAGCCTCACTGAGGCCAACGGCACCGGCGCCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGac                                                                                                                      <  2:56881/135‑1 (MQ=255)
        ccccAACCTGAGCCTCACTGAGGCCAACGGCACCGGCGCCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGaccac                                                                                                                   <  4:169058/135‑1 (MQ=255)
          ccAACCTGAGCCTCACTGAGGCCAACGGCACCGGCGCCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTAACAACAGCGTGGCAATCCCGACCAGCAGCCTGac                                                                                                                      <  4:47264/130‑1 (MQ=255)
          ccAACCTGAGCCTCACTGAGGCCAACGGCACCGGCGCCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTAACAACAGCGTGGCAATCCCGACCAGCAGCCTGac                                                                                                                      >  3:47264/1‑130 (MQ=255)
            aaCCTGAGCCTCACTGAGGCCAACGGCACCGGCGCCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGc                                                                                                               <  8:63828/135‑1 (MQ=255)
            aaCCTGAGCCTCACTGAGGCCAACGGCACCGGCGCCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGc                                                                                                               <  6:28297/135‑1 (MQ=255)
                     cTCACAGAGGCCAACGGCACCGGCGCCTGGTACGCCCAGTCAGCCCTCAACACGTCCGCAAGAACCATCGTGACACTGTCCTCTCACAACAGAGAGTCAATCCCGACCAGCAGCCAGACCACCAGCAACCTTccc                                                                                                      >  5:214127/1‑135 (MQ=255)
                           gAGGCCAACGGCACCGGCGCCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGCCCAGCAGCCTGACCAACAGCACCGTTCCCCTgacc                                                                                                >  1:286832/1‑135 (MQ=255)
                             tgCCAACGGCACCGGCGCCTGGTACGCACAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACGAGCAGCCTGACCACCAGCAACGTTCCCCTgacc                                                                                                <  6:145786/132‑1 (MQ=255)
                                gaaCGGGAGCGGCTCGTGGTACGCGCAGTCAGCCGTCAACCCGGCGTCCGTTACCATGGTGACATTGTCGTCTTACAACAGGTTGGCTATCCCGACCTGCAGCCTGACCAGCAGCAACGTTCCCCTGACGGACCt                                                                                           <  8:198263/134‑1 (MQ=255)
                                cAACGGCACCGGCGCCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCt                                                                                           <  6:246948/135‑1 (MQ=255)
                                cAACGGCACCGGCGCCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCt                                                                                           <  1:128469/135‑1 (MQ=255)
                                    ggCACCGGCGCCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCCCCAGCAACGTTCCCCTGACCGACCTGGTc                                                                                       >  2:174841/1‑135 (MQ=255)
                                    ggCACCGGCGCCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTc                                                                                       >  7:217542/1‑135 (MQ=255)
                                    ggCACCGGCGCCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTc                                                                                       <  4:263344/135‑1 (MQ=255)
                                           gcgcCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCa                                                                                <  4:261041/135‑1 (MQ=255)
                                           gcgcCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCa                                                                                <  1:18545/135‑1 (MQ=255)
                                           gcgcCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCa                                                                                <  6:13489/135‑1 (MQ=255)
                                           gcgcCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCa                                                                                <  3:130577/135‑1 (MQ=255)
                                           gcgcCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCa                                                                                <  6:26495/135‑1 (MQ=255)
                                           gcgcCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCAACCAGCAGCCTGACCACCAGCAACGTTCCCCTGCCCGACCTGGTCACCATCa                                                                                >  6:185741/1‑135 (MQ=255)
                                           gcgcCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCAACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCa                                                                                <  5:185741/135‑1 (MQ=255)
                                           gcgcCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCAACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCa                                                                                <  5:189356/135‑1 (MQ=255)
                                           gcgcCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCAACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCa                                                                                >  6:189356/1‑135 (MQ=255)
                                              ccTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCACGc                                                                             <  4:204211/135‑1 (MQ=255)
                                                               gCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCACGCAGGCCCAGTACCGCCTg                                                            >  5:198299/1‑135 (MQ=255)
                                                                      aCCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCACGCAGGCCCAGTACCGCCTGTCCAc                                                       <  8:251235/133‑1 (MQ=255)
                                                                        ccGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCACGCAGGCCCAGTACCGCCTGTCCACCgg                                                    >  7:83566/1‑134 (MQ=255)
                                                                         cggcggCCGGAACCATCGTGACACTGTCCGATGCCAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGCCCTGGTCACCATCACGCAGGCCCAGTACCGCCTGTCCACCgg                                                    >  4:208107/1‑133 (MQ=255)
                                                                            cggcCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCACGCAGGCCCAGTACCGCCTGTCCACCGGCGAGc                                               <  8:10182/135‑1 (MQ=255)
                                                                             ggcCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCACGCAGGCCCAGTACCGCCTGTCCACCGGCGAGc                                               >  4:268612/1‑134 (MQ=255)
                                                                                 ggAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCACGCAGGCCCAGTACCGCCTGTCCACCGGCGAGCTGAcc                                          <  6:198299/135‑1 (MQ=255)
                                                                                      cATCGGGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCACGCAGGCCCAGTACCGCCTGTCCACCGGCGAGCTGACCCtggt                                     <  5:47368/135‑1 (MQ=255)
                                                                                            gACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCACGCAGGCCCAGTACCGCCTGTCCACCGGCGAGCTGACCCTGGTGGCCAg                               >  8:10343/1‑135 (MQ=255)
                                                                                                     cGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCACGCAGGCCCAGAACCGCCTGTCCACCGGCGAGCTGACCCTGGTGGCCAGCACCAgagc                      >  5:20988/1‑132 (MQ=255)
                                                                                                      gCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGCCCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCACGCAGGCCCAGTACCGCCTGTCCACCGGCGAGCTGCCCCTGGTGGCCAGCACCAGTGa                      >  8:74310/1‑134 (MQ=255)
                                                                                                      gCTCACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCa                                                                                >  2:157504/1‑76 (MQ=255)
                                                                                                      gCTCACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCa                                                                                <  1:157504/76‑1 (MQ=255)
                                                                                                        tGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCa                                                                                                              <  2:36888/44‑1 (MQ=255)
                                                                                                        tGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCa                                                                                                              >  1:36888/1‑44 (MQ=255)
                                                                                                        tGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCACGCAGGCCCAGTACCGCCTGTCCACCGGCGAGCTGACCCTGGTGGCCAGCACCAGTGACGa                   <  8:3131/135‑1 (MQ=255)
                                                                                                           caacaTCGTGTCAATCCCGACCAGCAGCATTACCACCAGCAACGTTCCCATGAGCGACCTGGTCACCATCACGCAGGCCCAGTACCGCCTGTCCACCGGCGAGCTGACCCTGTTGGCCAGCACCAGTGACGAaac                <  5:197757/135‑1 (MQ=255)
                                                                                                           caacaGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCACGCAGGCCCAGTACCGCCTGTCCACCGGCGAGCTGACCCTGGTGGCCAGCACCAGTGACGAaac                <  7:10343/135‑1 (MQ=255)
                                                                                                           caacaGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCACGCAGGCCCAGTACCGCCTGTCCACCGGCGAGCTGACCCTGGTGGCCAGCACCAGTGACGAaac                <  3:171091/135‑1 (MQ=255)
                                                                                                                gCGTGGCAATCCCGACCAGCATCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCACGCAGGCTCAGTACCGACTGTCCACCGGCGAGCTGACCCTGGTGGAAATCACCATTGACGAAACAacac           <  6:214127/135‑1 (MQ=255)
                                                                                                                   tGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCACGCa                                                                            <  4:55451/67‑1 (MQ=255)
                                                                                                                   tGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCACGCa                                                                            >  3:55451/1‑67 (MQ=255)
                                                                                                                    ggCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCACGCAGGCCCAGTACCGCCTGTCCACCGGCGAGCTGACCCTGGTGGCCAGCACCAGTGACGAAACAACACCTcc       <  2:14009/135‑1 (MQ=255)
                                                                                                                         tCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCACGCAGGCCCAGTACCGCCTGTCCACCGGCGAGCTGACCCTGGTGGCCAGCCCCAGTGACGAACCAACACCTCCAGtcc  >  2:137988/1‑133 (MQ=255)
                                                                                                                             |                                                                                                                                  
AGCCAGACCCCCAACCTGAGCCTCACTGAGGCCAACGGCACCGGCGCCTGGTACGCCCAGTCAGCCCTCAACCCGGCGGCCGGAACCATCGTGACACTGTCCGCTGACAACAGCGTGGCAATCCCGACCAGCAGCCTGACCACCAGCAACGTTCCCCTGACCGACCTGGTCACCATCACGCAGGCCCAGTACCGCCTGTCCACCGGCGAGCTGACCCTGGTGGCCAGCACCAGTGACGAAACAACACCTCCAGTAC  >  ACB122/3841468‑3841723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑