| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 3,895,319 | C→T | 9.5% | R118C (CGC→TGC) | CJ019_03685 → | putative protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,895,319 | 0 | C | T | 9.5% | 119.9 / 4.0 | 42 | R118C (CGC→TGC) | CJ019_03685 | putative protein |
| Reads supporting (aligned to +/- strand): ref base C (25/13); new base T (2/2); total (27/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.97e-01 | |||||||||||
CAGAAGCTCAACGGTGCGGGTGTTGGCCTGCACTTGTATGTTGAGGACTGCGACAAGGTCTACGCCCAGGCCCTGGCCGCCGGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGTCCACCCACAAAGAGGACCTGACCCCCGACGAGATTCGCGCCCGTGCCGCGAAGATGTTCGGCGGTAGCCAAGCGA > ACB122/3895193‑3895439 | cagAAGCTCAACGGTGCGGGTGTTGGCCTGCACTTGTATGTTGAGGACTGCGACAAGGTCTACGCCCAGGCCCTGGCCGCCGGGGGCATCCAGTTACATCCCTTGCCCGACCAGTTTTACGGTGACCGCAGCGGc > 1:103051/1‑135 (MQ=255) cGGTGCGGGTGTTGGCCTGCACTTGTATGTTGAGGACTGCGACAAGGTCTACGCCCAGGCCCTGGCCGCCGGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGCCCGCAGCGGCCCCCTg > 2:148759/1‑130 (MQ=255) tGGCCTGCACTTGTATGTTGAGGACTGCGACAAGGTCTACGCCCAGGCCCTGGCCGCCGGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCaa < 5:229225/135‑1 (MQ=255) ttGTATGTTGAGGACTGCGACAAGGTCTACGCCCAGGCCCTGGCCGCCGGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCCCCCTGAAAGATCCGTTTGGCAACATCTGGTTc > 5:144590/1‑135 (MQ=255) ttGTATGTTGAGGACTGCGACAAGGTCTACGCCCAGGCCCTGGCCGCCGGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTc > 8:86093/1‑135 (MQ=255) gTTGAGGACTGCGACAAGGTCTACGCCCAGGCCCTGGCCGCCGGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGTcc > 1:158563/1‑135 (MQ=255) tGAGTACTGCGACAAGGTCTACGCCCAGGCCCTGGCCGCCGGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGTcca < 5:165809/134‑1 (MQ=255) ggACTGCGACAAGGTCTACGCCCAGGCCCTGGCCGCCGGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGTccaccc > 8:87025/1‑134 (MQ=255) gACTGCGACAAGGGCTACGCCCAGGCCCTGGCCGCCGGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGTccacccac < 8:207969/135‑1 (MQ=255) aCTGCGACAAGGTCTACGCCCAGGCCCTGGCCGCCGGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGttt > 8:20076/1‑107 (MQ=255) aCTGCGACAAGGTCTACGCCCAGGCCCTGGCCGCCGGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGttt < 7:20076/107‑1 (MQ=255) gACAAGGTCTACGCCCAGGCCCTGGCCGCCGGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCgt < 3:297527/119‑1 (MQ=255) gACAAGGTCTACGCCCAGGCCCTGGCCGCCGGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCgt > 4:297527/1‑119 (MQ=255) cTACGCCCAGGCCCTGGCCGCCGGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGCCCGCAGCGGCACCCTGAAAGATCCGATTGGCAACATCTGGTTCGTCTCAACCCACAAAGAGGACCTGAc > 3:268866/1‑135 (MQ=255) gCCCAGGCCCTGGCCGCCGGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGTCCACCCACAAAGAGGACCTGAccccc > 1:55518/1‑135 (MQ=255) gCCCAGGCCCTGGCCGCCGGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGCTGCCCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGACCACCCACAAAGAGGACCTGAcgcc > 7:60586/1‑134 (MQ=255) cccAGGCCCTGGCCGCCGGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGTCCACCCACAAAGAGGACCTGACCCCcg < 4:161311/135‑1 (MQ=255) ccAGGCCCTGGCCGCCGGGGGCATCCAGTTACATCCCTTGCCCGACCAGTTTTACGGTGCCCGCAGCGGCACCCTGAAAGATCCGTTTGGCACCATCTGGTTCGTGTCCACCCACAAAGAGGACCTGACCCCcga > 8:281020/1‑135 (MQ=255) cccTGGCCGCCGGGGGCATCCAGTTACATCCCTTGCCCGCCCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGAGCCGTTTGGCGACATCTGGTTCGTGTCCACCCACCAAGAGGACCTGCCCCCCGAAgaga > 5:167846/1‑135 (MQ=255) ccTGGCCGCCGGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGTCCACCCACAAAGAGGACCTGACCCCCGACGAGAt > 8:202147/1‑135 (MQ=255) ccTGGCCGCCGGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGTCCACCCACAAAGAGGACCTGACCCCCGACGAGAt > 2:21989/1‑135 (MQ=255) ccTGGCCGCCGGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGTCCACACACAAAGAGGACCTGAACCCCGACGAGAt > 3:201550/1‑135 (MQ=255) cgccgGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAATATCCGTTTGGCAACATCTGGTTCGGGTCCACCCACAAAGAGGACCTGACCCCCGACGAGATTCgcgc < 5:151835/135‑1 (MQ=255) cgccgGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGGCCACCCACAAAGAGGACCTGACCCCCGACGAGATTCgcgc < 8:252534/135‑1 (MQ=255) gccgGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGTCCACCCACCAAGAGGACCTGCCCCCCGACGAGATTCGCGcc > 7:248895/1‑135 (MQ=255) gccgGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGTCCACCCACAAAGAGGCCCTGACCCCCGACGAGATTCGCGcc > 4:165883/1‑135 (MQ=255) gggCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGTCCACCCACAAAGAGGACCTGACCCCCGACGAGATTCGCGCCCGTGc > 3:119222/1‑135 (MQ=255) aTCCAGTTACATCCCCTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGTCCACCCACAAAGAGGACCTGACCCCCGACGAGATTCGCGCCCGTGCcgcg < 8:208363/135‑1 (MQ=255) gTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGGCCACCCACAAAGAGGACCTGACCCCCGACGAGATTCGCGCCCGTGCCGCGAAGAt < 7:86093/135‑1 (MQ=255) ccgaccAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGTCCACCCACAAAGAGGACCTGACCCCCGACGAGATTCGCGCCCGTGCCGCGAAGATGTTCGGCGGTAGc > 5:242423/1‑134 (MQ=255) ccgaccAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGTCCACCCACAAAGAGGACCTGACCCCCGACGAGATTCGCGCCCGTGCCGCGAAGATGTTCGGCGGTAGc > 8:49037/1‑134 (MQ=255) ccAGTTTTCCGGTGACCGCAGCGGCACCCTGAACGAGCCGTTTGGCAACATCTGGTGCGTGCCCACCCACAACGAGGACCTGACCCCCGCCGAGATGCGCGCCCGTGCCGCGAAGATGTTCGGCGGTAGCCAAGc < 3:165883/135‑1 (MQ=255) ccAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGTCCACCCACAAAGAGGACCTGACCCCCGACGAGATTCGCGCCCGTGCCGCGAAGATGTTCGGCGGTAGCCAAGc < 7:202147/135‑1 (MQ=255) ccAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCCTTTGGCAACAACTGGTTCGTGTCCACCCCCAAAGACGCCCTGACCCCCCACGAGATTCGCGCCCGTGCCGCGAAGATCTTCGGCGGTAGCCAAGc > 2:187774/1‑135 (MQ=255) aGTTTTACGGTGACTGCAGCGGCACCCTGAAAGATc < 2:132545/36‑1 (MQ=255) aGTTTTACGGTGACTGCAGCGGCACCCTGAAAGATc < 2:128680/36‑1 (MQ=255) aGTTTTACGGTGACTGCAGCGGCACCCTGAAAGATc > 1:132545/1‑36 (MQ=255) aGTTTTACGGTGACTGCAGCGGCACCCTGAAAGATc > 1:128680/1‑36 (MQ=255) aGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCgt < 3:73009/58‑1 (MQ=255) aGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCgt > 4:73009/1‑58 (MQ=255) aGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGTCCACCCACAAAGAGGACCTGACCCCCGACGAGATTCGCGCCCGTGCCGCGAAGATGTTCGGCGGTAGCCAAGcga > 7:161401/1‑135 (MQ=255) aGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGTCCACCCACAAAGAGGACCTGACCCCCGACGAGATTCGCGCCCGTGCCGCGAAGATGTTCGGCGGTAGCCAAGcga > 8:293203/1‑135 (MQ=255) | CAGAAGCTCAACGGTGCGGGTGTTGGCCTGCACTTGTATGTTGAGGACTGCGACAAGGTCTACGCCCAGGCCCTGGCCGCCGGGGGCATCCAGTTACATCCCTTGACCGACCAGTTTTACGGTGACCGCAGCGGCACCCTGAAAGATCCGTTTGGCAACATCTGGTTCGTGTCCACCCACAAAGAGGACCTGACCCCCGACGAGATTCGCGCCCGTGCCGCGAAGATGTTCGGCGGTAGCCAAGCGA > ACB122/3895193‑3895439 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |